RchiOBHm_Chr1g0349161

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
N/A
Physical Location & Seq
Forward (+)
42232103 .. 42234562
2460 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGCTTCTCATTTTCCCGCCAAAACAACTTTCCCAACTTGAAAACGAAAATCGAAGATCGAAACTTTCTGAAGAGAGAGAGAGAGAGAGAGAGAGAGATGGAGGCGGAGAAAGCAGGGGCATCGCTGGACTAGCTGATCGCCGGGTTCAACAGTCGGATCTCGGAGCTCCAAGAGCTCGTTATTGCGCGAAACACACCGTCTCCGATTTGTCGGCCGTCGATGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCGGGTTCGCGACGAAACCCTTGCCATACCCAAAGCAAAAGTAAAACTGATCGATGAATCGCTGAAGCAGCGAAGGAAATTGAAGGATATGTCAATTTATGCCCCCAATCATGTTCCTGAAAGAATGTCGATGTTGAATTTGGATACAAATAGATGTTTATTTCCAGAAAGCTCTCAGCAAAACACTGGTTCTGGGACCTTCAAGCTTGAGGAGGATCCTGCACCACCACCACCACCCAAGGAGAAAAAGGATCGTTCCATGCCACCATTGTGGTTTGTAAGTGCTGAAGAGCTGGATTCCTTGTCATCGTCTGATTTTGGTTATCAATTATCATGCGGGTGGCAGGGGATCGATGGGAAAAAACCTTGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

213

Amino Acids

24.05

Weight (kDa)

8.7

Isoelectric Point (pI)

57.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 64 - 192 1e-20 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 188, 276
AciI CCGC 4 cut(s) 17, 105, 225, 603
AclWI GGATC 5 cut(s) 165, 476, 489, 525, 623
AcoI YGGCCR 1 cut(s) 213
AcsI RAATTY 1 cut(s) 403
AcuI CTGAAG 4 cut(s) 90, 230, 350, 573
AgsI TTSAA 5 cut(s) 41, 149, 349, 403, 469
AjnI CCWGG 1 cut(s) 252
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AleI CACNNNNGTG 1 cut(s) 535
AluBI AGCT 7 cut(s) 134, 167, 176, 244, 438, 472, 559
AluI AGCT 7 cut(s) 134, 167, 176, 244, 438, 472, 559
Alw21I GWGCWC 2 cut(s) 169, 178
Alw26I GTCTC 1 cut(s) 205
AlwI GGATC 5 cut(s) 165, 476, 489, 525, 623
AoxI GGCC 3 cut(s) 213, 234, 255
ApeKI GCWGC 1 cut(s) 334
ApoI RAATTY 1 cut(s) 403
AspLEI GCGC 1 cut(s) 188
AspS9I GGNCC 3 cut(s) 250, 265, 462
AsuC2I CCSGG 2 cut(s) 143, 269
AvaII GGWCC 3 cut(s) 250, 265, 462
BamHI GGATCC 1 cut(s) 481
BanII GRGCYC 2 cut(s) 169, 178
Bbv12I GWGCWC 2 cut(s) 169, 178
BbvI GCAGC 1 cut(s) 346
BccI CCATC 3 cut(s) 92, 266, 614
BceAI ACGGC 2 cut(s) 200, 212
BcgI CGANNNNNNTGC 4 cut(s) 103, 137, 269, 303
BciT130I CCWGG 1 cut(s) 254
BciVI GTATCC 1 cut(s) 403
BcnI CCSGG 2 cut(s) 143, 269
BcoDI GTCTC 1 cut(s) 205
BfaI CTAG 1 cut(s) 131
BfuI GTATCC 1 cut(s) 403
BglI GCCNNNNNGGC 1 cut(s) 233
BisI GCNGC 2 cut(s) 225, 335
BlsI GCNGC 2 cut(s) 226, 336
Bme1390I CCNGG 3 cut(s) 143, 254, 269
Bme18I GGWCC 3 cut(s) 250, 265, 462
BmgT120I GGNCC 3 cut(s) 250, 265, 462
BmiI GGNNCC 2 cut(s) 463, 483
BmrFI CCNGG 3 cut(s) 143, 254, 269
BmsI GCATC 2 cut(s) 129, 211
BpuEI CTTGAG 1 cut(s) 494
BpuMI CCSGG 2 cut(s) 143, 269
Bsa29I ATCGAT 2 cut(s) 318, 618
BsaJI CCNNGG 3 cut(s) 237, 504, 632
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bse1I ACTGG 1 cut(s) 457
BseBI CCWGG 1 cut(s) 254
BseCI ATCGAT 2 cut(s) 318, 618
BseDI CCNNGG 3 cut(s) 237, 504, 632
BseMII CTCAG 1 cut(s) 455
BseNI ACTGG 1 cut(s) 457
BseRI GAGGAG 1 cut(s) 491
BseX3I CGGCCG 1 cut(s) 213
BseXI GCAGC 1 cut(s) 346
BsgI GTGCAG 1 cut(s) 471
Bsh1236I CGCG 2 cut(s) 188, 276
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 3 cut(s) 215, 236, 257
BshVI ATCGAT 2 cut(s) 318, 618
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 2 cut(s) 169, 178
BsiSI CCGG 2 cut(s) 142, 268
BslFI GGGAC 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 205
BsmBI CGTCTC 1 cut(s) 205
BsmFI GGGAC 1 cut(s) 475
BsnI GGCC 3 cut(s) 215, 236, 257
Bsp1286I GDGCHC 2 cut(s) 169, 178
Bsp143I GATC 7 cut(s) 56, 136, 157, 315, 481, 517, 615
Bsp19I CCATGG 1 cut(s) 237
Bsp68I TCGCGA 1 cut(s) 276
BspACI CCGC 4 cut(s) 17, 105, 225, 603
BspANI GGCC 3 cut(s) 215, 236, 257
BspCNI CTCAG 1 cut(s) 454
BspDI ATCGAT 2 cut(s) 318, 618
BspFNI CGCG 2 cut(s) 188, 276
BspLI GGNNCC 2 cut(s) 463, 483
BspPI GGATC 5 cut(s) 165, 476, 489, 525, 623
BspQI GCTCTTC 1 cut(s) 549
BsrI ACTGG 1 cut(s) 457
BssECI CCNNGG 3 cut(s) 237, 504, 632
BssMI GATC 7 cut(s) 56, 136, 157, 315, 481, 517, 615
BssT1I CCWWGG 3 cut(s) 237, 504, 632
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 2 cut(s) 153, 199
Bst6I CTCTTC 2 cut(s) 66, 549
BstDEI CTNAG 1 cut(s) 441
BstDSI CCRYGG 1 cut(s) 237
BstFNI CGCG 2 cut(s) 188, 276
BstHHI GCGC 1 cut(s) 188
BstKTI GATC 7 cut(s) 59, 139, 160, 318, 484, 520, 618
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 7 cut(s) 56, 136, 157, 315, 481, 517, 615
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 6 cut(s) 111, 131, 173, 221, 233, 334
BstNI CCWGG 1 cut(s) 254
BstSCI CCNGG 3 cut(s) 141, 252, 267
BstUI CGCG 2 cut(s) 188, 276
BstV1I GCAGC 1 cut(s) 346
BstX2I RGATCY 2 cut(s) 157, 481
BstXI CCANNNNNNTGG 1 cut(s) 537
BstYI RGATCY 2 cut(s) 157, 481
BstZI CGGCCG 1 cut(s) 213
Bsu15I ATCGAT 2 cut(s) 318, 618
BsuI GTATCC 1 cut(s) 403
BsuRI GGCC 3 cut(s) 215, 236, 257
BsuTUI ATCGAT 2 cut(s) 318, 618
BtgI CCRYGG 1 cut(s) 237
BtgZI GCGATG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 450
BtuMI TCGCGA 1 cut(s) 276
CfoI GCGC 1 cut(s) 188
Cfr13I GGNCC 3 cut(s) 250, 265, 462
ClaI ATCGAT 2 cut(s) 318, 618
CviAII CATG 4 cut(s) 238, 377, 526, 600
DdeI CTNAG 1 cut(s) 441
DpnI GATC 7 cut(s) 58, 138, 159, 317, 483, 519, 617
DpnII GATC 7 cut(s) 56, 136, 157, 315, 481, 517, 615
EaeI YGGCCR 1 cut(s) 213
EagI CGGCCG 1 cut(s) 213
Eam1104I CTCTTC 2 cut(s) 66, 549
EarI CTCTTC 2 cut(s) 66, 549
EciI GGCGGA 1 cut(s) 120
Ecl136II GAGCTC 2 cut(s) 167, 176
EclXI CGGCCG 1 cut(s) 213
Eco130I CCWWGG 3 cut(s) 237, 504, 632
Eco24I GRGCYC 2 cut(s) 169, 178
Eco47I GGWCC 3 cut(s) 250, 265, 462
Eco52I CGGCCG 1 cut(s) 213
Eco53kI GAGCTC 2 cut(s) 167, 176
Eco57I CTGAAG 4 cut(s) 90, 230, 350, 573
EcoICRI GAGCTC 2 cut(s) 167, 176
EcoO109I RGGNCCY 1 cut(s) 462
EcoRII CCWGG 1 cut(s) 252
EcoT14I CCWWGG 3 cut(s) 237, 504, 632
EcoT38I GRGCYC 2 cut(s) 169, 178
ErhI CCWWGG 3 cut(s) 237, 504, 632
Esp3I CGTCTC 1 cut(s) 205
FaeI CATG 4 cut(s) 241, 380, 529, 603
FaiI YATR 7 cut(s) 239, 293, 356, 366, 378, 527, 601
FaqI GGGAC 1 cut(s) 475
FatI CATG 4 cut(s) 237, 376, 525, 599
FauI CCCGC 2 cut(s) 24, 596
Fnu4HI GCNGC 2 cut(s) 225, 335
FriOI GRGCYC 2 cut(s) 169, 178
Fsp4HI GCNGC 2 cut(s) 225, 335
FspBI CTAG 1 cut(s) 131
GlaI GCGC 1 cut(s) 187
GluI GCNGC 2 cut(s) 225, 335
HaeIII GGCC 3 cut(s) 215, 236, 257
HapII CCGG 2 cut(s) 142, 268
HhaI GCGC 1 cut(s) 188
Hin1II CATG 4 cut(s) 241, 380, 529, 603
Hin6I GCGC 1 cut(s) 186
HinP1I GCGC 1 cut(s) 186
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 2 cut(s) 323, 563
HpaII CCGG 2 cut(s) 142, 268
Hpy188I TCNGA 5 cut(s) 70, 157, 164, 205, 580
Hpy188III TCNNGA 3 cut(s) 275, 383, 431
Hpy99I CGWCG 2 cut(s) 221, 281
HpyAV CCTTC 4 cut(s) 226, 333, 343, 475
HpyCH4III ACNGT 2 cut(s) 153, 199
HpyCH4V TGCA 1 cut(s) 488
HpyF10VI GCNNNNNNNGC 6 cut(s) 111, 131, 173, 221, 233, 334
HpyF3I CTNAG 1 cut(s) 441
Hsp92II CATG 4 cut(s) 241, 380, 529, 603
HspAI GCGC 1 cut(s) 186
Kzo9I GATC 7 cut(s) 56, 136, 157, 315, 481, 517, 615
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 3 cut(s) 164, 172, 241
Lsp1109I GCAGC 1 cut(s) 346
LweI GCATC 2 cut(s) 129, 211
MaeI CTAG 1 cut(s) 131
MalI GATC 7 cut(s) 58, 138, 159, 317, 483, 519, 617
MboI GATC 7 cut(s) 56, 136, 157, 315, 481, 517, 615
MboII GAAGA 3 cut(s) 66, 83, 566
MflI RGATCY 2 cut(s) 157, 481
MhlI GDGCHC 2 cut(s) 169, 178
MluCI AATT 4 cut(s) 344, 360, 403, 593
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 3 cut(s) 95, 469, 472
MslI CAYNNNNRTG 2 cut(s) 535, 604
MspI CCGG 2 cut(s) 142, 268
MspR9I CCNGG 3 cut(s) 143, 254, 269
MvaI CCWGG 1 cut(s) 254
MvnI CGCG 2 cut(s) 188, 276
MwoI GCNNNNNNNGC 6 cut(s) 111, 131, 173, 221, 233, 334
NciI CCSGG 2 cut(s) 143, 269
NcoI CCATGG 1 cut(s) 237
NdeII GATC 7 cut(s) 56, 136, 157, 315, 481, 517, 615
NlaIII CATG 4 cut(s) 241, 380, 529, 603
NlaIV GGNNCC 2 cut(s) 463, 483
NruI TCGCGA 1 cut(s) 276
OliI CACNNNNGTG 1 cut(s) 535
PciSI GCTCTTC 1 cut(s) 549
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PfeI GAWTC 2 cut(s) 323, 563
PkrI GCNGC 2 cut(s) 226, 336
PpuMI RGGWCCY 1 cut(s) 462
Psp124BI GAGCTC 2 cut(s) 169, 178
Psp5II RGGWCCY 1 cut(s) 462
Psp6I CCWGG 1 cut(s) 252
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 2 cut(s) 463, 483
PspPI GGNCC 3 cut(s) 250, 265, 462
PspPPI RGGWCCY 1 cut(s) 462
PsuI RGATCY 2 cut(s) 157, 481
RruI TCGCGA 1 cut(s) 276
RseI CAYNNNNRTG 2 cut(s) 535, 604
SacI GAGCTC 2 cut(s) 169, 178
SapI GCTCTTC 1 cut(s) 549
SatI GCNGC 2 cut(s) 225, 335
Sau3AI GATC 7 cut(s) 56, 136, 157, 315, 481, 517, 615
Sau96I GGNCC 3 cut(s) 250, 265, 462
ScrFI CCNGG 3 cut(s) 143, 254, 269
SduI GDGCHC 2 cut(s) 169, 178
SfaNI GCATC 2 cut(s) 129, 211
SinI GGWCC 3 cut(s) 250, 265, 462
SmiMI CAYNNNNRTG 2 cut(s) 535, 604
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 4 cut(s) 344, 360, 403, 593
SsiI CCGC 4 cut(s) 17, 105, 225, 603
SspMI CTAG 1 cut(s) 131
SstI GAGCTC 2 cut(s) 169, 178
StyD4I CCNGG 3 cut(s) 141, 252, 267
StyI CCWWGG 3 cut(s) 237, 504, 632
TaaI ACNGT 2 cut(s) 153, 199
TaqI TCGA 6 cut(s) 52, 59, 219, 318, 395, 618
TasI AATT 4 cut(s) 344, 360, 403, 593
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 2 cut(s) 323, 563
TscAI CASTG 1 cut(s) 457
TseI GCWGC 1 cut(s) 334
TspDTI ATGAA 1 cut(s) 336
TspRI CASTG 1 cut(s) 457
VpaK11BI GGWCC 3 cut(s) 250, 265, 462
XapI RAATTY 1 cut(s) 403
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.