RchiOBHm_Chr1g0349201

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
N/A
Physical Location & Seq
Forward (+)
42258819 .. 42265975
7157 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 915 bp
ATGGAGGCGGAGAAAGCAGGGGCGTCGTTGGACTCGCTGATCGCCGGGTTTAACAGTCGGATCTCGGAGCTCCAAGAGCTCGCTATTGCCCGAAACATGTACCCGGCCAGCAGCGTCACCGATTTGACGTCCGTCGACGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCGGGTTCGCGACGAAACCCTAGCCATCCCCAAAGAAAAAGTAGTTCCTTTTCTCTCTCTATATGACTATATGTCGATTGGGCTTTGTTTGATTTCTGAGAAATTTTATGAAAATGTTGATATATCAGTATGCGAGGTCCTTATAATTTTAATCATGTTTTGGGTGGAGTTTGTGATAATCGTGCTTGTGGCTACTTGGAAAATCAATTTACTAAGTGAATTGGAAAGTAAAAGAAGCAATTTTGATTATCTGTTTATTTCCAGAAAGCTCTCAGCAAAACAATGGTTTGGTTTTGGGACCTTCAAGCTTGAGAAGGATCCTGCACCGCCGCCTAAAGAGAAAAAGGGTCGTTCCACACCCCCATTGTGGTTTAGAAGTGCTGAAGAGCTGGAATGCTGGATTCCTTGTCATCGCTGTTGTGTATCTACTCCATGGTGGGTAAGTATTGAAAAAAGAGGTCTTCTGTATCTAGCTTTGTTATGGCGTCATTTTCTTGCTCAGAGTATTACTAACTGCATATACATGAGAGGAAGGCTTACACTAGAGAAGGTCAACACAGCTATTAATGACATGGCAACATATGCTGAAGCAAATTATCAGCTCATAGCTGCTCCAAAGAAGAATGTGGCAGGAGATCGTTTGGAAAAGGCCTTGGTCCTTCGTCACCTTGGTCGTGTAAGTGAGACACGGATTGGTCATCATCAGGTGATCATTCTTTTGAAGCCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

304

Amino Acids

34.68

Weight (kDa)

8.58

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 74 2.3e-13 Spindle and kinetochore-associated protein 1
SKA1 PF07160 166 - 277 9.5e-14 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 326
AatII GACGTC 1 cut(s) 131
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 192
AciI CCGC 4 cut(s) 8, 141, 509, 512
AclWI GGATC 3 cut(s) 68, 494, 507
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 1 cut(s) 284
AcuI CTGAAG 3 cut(s) 146, 585, 789
AcyI GRCGYC 4 cut(s) 23, 128, 138, 667
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 549
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 3 cut(s) 487, 632, 904
AhdI GACNNNNNGTC 1 cut(s) 253
AjnI CCWGG 1 cut(s) 168
Alw21I GWGCWC 2 cut(s) 72, 81
Alw26I GTCTC 1 cut(s) 860
AlwI GGATC 3 cut(s) 68, 494, 507
AoxI GGCC 4 cut(s) 105, 150, 171, 831
ApeKI GCWGC 2 cut(s) 111, 791
ApoI RAATTY 1 cut(s) 284
AseI ATTAAT 1 cut(s) 747
AspS9I GGNCC 5 cut(s) 166, 181, 319, 480, 838
AsuC2I CCSGG 3 cut(s) 46, 104, 185
AsuHPI GGTGA 3 cut(s) 109, 839, 901
AvaII GGWCC 5 cut(s) 166, 181, 319, 480, 838
BamHI GGATCC 1 cut(s) 499
BanII GRGCYC 2 cut(s) 72, 81
BbsI GAAGAC 1 cut(s) 635
Bbv12I GWGCWC 2 cut(s) 72, 81
BbvI GCAGC 2 cut(s) 123, 778
BccI CCATC 2 cut(s) 182, 215
BceAI ACGGC 1 cut(s) 128
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 1 cut(s) 170
BclI TGATCA 1 cut(s) 891
BcnI CCSGG 3 cut(s) 46, 104, 185
BcoDI GTCTC 1 cut(s) 860
BfaI CTAG 3 cut(s) 203, 653, 725
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 4 cut(s) 112, 141, 512, 792
BlsI GCNGC 4 cut(s) 113, 142, 513, 793
Bme1390I CCNGG 4 cut(s) 46, 104, 170, 185
Bme18I GGWCC 5 cut(s) 166, 181, 319, 480, 838
BmeRI GACNNNNNGTC 1 cut(s) 253
BmgT120I GGNCC 5 cut(s) 166, 181, 319, 480, 838
BmiI GGNNCC 2 cut(s) 481, 501
BmrFI CCNGG 4 cut(s) 46, 104, 170, 185
BoxI GACNNNNGTC 1 cut(s) 131
BpiI GAAGAC 1 cut(s) 635
BpuEI CTTGAG 1 cut(s) 512
BpuMI CCSGG 3 cut(s) 46, 104, 185
BsaHI GRCGYC 4 cut(s) 23, 128, 138, 667
BsaJI CCNNGG 4 cut(s) 153, 614, 834, 850
Bsc4I CCNNNNNNNGG 1 cut(s) 549
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 4 cut(s) 153, 614, 834, 850
BseGI GGATG 1 cut(s) 207
BseLI CCNNNNNNNGG 1 cut(s) 549
BseMII CTCAG 3 cut(s) 270, 468, 695
BseXI GCAGC 2 cut(s) 123, 778
BsgI GTGCAG 1 cut(s) 489
Bsh1236I CGCG 1 cut(s) 192
BshFI GGCC 4 cut(s) 107, 152, 173, 833
BsiHKAI GWGCWC 2 cut(s) 72, 81
BsiSI CCGG 3 cut(s) 45, 104, 184
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 1 cut(s) 549
BsmAI GTCTC 1 cut(s) 860
BsmFI GGGAC 1 cut(s) 493
BsmI GAATGC 1 cut(s) 581
BsnI GGCC 4 cut(s) 107, 152, 173, 833
Bsp1286I GDGCHC 2 cut(s) 72, 81
Bsp143I GATC 5 cut(s) 39, 60, 499, 817, 891
Bsp19I CCATGG 2 cut(s) 153, 614
Bsp68I TCGCGA 1 cut(s) 192
BspACI CCGC 4 cut(s) 8, 141, 509, 512
BspANI GGCC 4 cut(s) 107, 152, 173, 833
BspCNI CTCAG 3 cut(s) 271, 467, 694
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 2 cut(s) 481, 501
BspPI GGATC 3 cut(s) 68, 494, 507
BspQI GCTCTTC 1 cut(s) 561
BssECI CCNNGG 4 cut(s) 153, 614, 834, 850
BssMI GATC 5 cut(s) 39, 60, 499, 817, 891
BssNI GRCGYC 4 cut(s) 23, 128, 138, 667
BssT1I CCWWGG 4 cut(s) 153, 614, 834, 850
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 1 cut(s) 56
Bst6I CTCTTC 1 cut(s) 561
BstACI GRCGYC 4 cut(s) 23, 128, 138, 667
BstAPI GCANNNNNTGC 1 cut(s) 764
BstC8I GCNNGC 2 cut(s) 81, 109
BstDEI CTNAG 4 cut(s) 279, 395, 454, 681
BstDSI CCRYGG 2 cut(s) 153, 614
BstF5I GGATG 1 cut(s) 207
BstFNI CGCG 1 cut(s) 192
BstKTI GATC 5 cut(s) 42, 63, 502, 820, 894
BstMAI GTCTC 1 cut(s) 860
BstMBI GATC 5 cut(s) 39, 60, 499, 817, 891
BstMWI GCNNNNNNNGC 4 cut(s) 14, 76, 149, 764
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 100
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 4 cut(s) 44, 102, 168, 183
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 2 cut(s) 123, 778
BstV2I GAAGAC 1 cut(s) 635
BstX2I RGATCY 2 cut(s) 60, 499
BstYI RGATCY 2 cut(s) 60, 499
BsuRI GGCC 4 cut(s) 107, 152, 173, 833
BtgI CCRYGG 2 cut(s) 153, 614
BtgZI GCGATG 1 cut(s) 578
BtsCI GGATG 1 cut(s) 207
BtuMI TCGCGA 1 cut(s) 192
Cac8I GCNNGC 2 cut(s) 81, 109
Cfr13I GGNCC 5 cut(s) 166, 181, 319, 480, 838
CseI GACGC 4 cut(s) 12, 103, 146, 656
Csp6I GTAC 1 cut(s) 100
CviAII CATG 6 cut(s) 97, 154, 337, 615, 706, 754
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 4 cut(s) 279, 395, 454, 681
DpnI GATC 5 cut(s) 41, 62, 501, 819, 893
DpnII GATC 5 cut(s) 39, 60, 499, 817, 891
DriI GACNNNNNGTC 1 cut(s) 253
EaeI YGGCCR 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 561
Eam1105I GACNNNNNGTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 561
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 2 cut(s) 70, 79
Eco130I CCWWGG 4 cut(s) 153, 614, 834, 850
Eco147I AGGCCT 1 cut(s) 833
Eco24I GRGCYC 2 cut(s) 72, 81
Eco47I GGWCC 5 cut(s) 166, 181, 319, 480, 838
Eco53kI GAGCTC 2 cut(s) 70, 79
Eco57I CTGAAG 3 cut(s) 146, 585, 789
EcoICRI GAGCTC 2 cut(s) 70, 79
EcoO109I RGGNCCY 2 cut(s) 319, 480
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 4 cut(s) 153, 614, 834, 850
EcoT38I GRGCYC 2 cut(s) 72, 81
ErhI CCWWGG 4 cut(s) 153, 614, 834, 850
FaeI CATG 6 cut(s) 100, 157, 340, 618, 709, 757
FaqI GGGAC 1 cut(s) 493
FatI CATG 6 cut(s) 96, 153, 336, 614, 705, 753
FauNDI CATATG 1 cut(s) 763
FbaI TGATCA 1 cut(s) 891
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 4 cut(s) 112, 141, 512, 792
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 2 cut(s) 72, 81
Fsp4HI GCNGC 4 cut(s) 112, 141, 512, 792
FspBI CTAG 3 cut(s) 203, 653, 725
GluI GCNGC 4 cut(s) 112, 141, 512, 792
HaeIII GGCC 4 cut(s) 107, 152, 173, 833
HapII CCGG 3 cut(s) 45, 104, 184
HgaI GACGC 4 cut(s) 12, 103, 146, 656
Hin1I GRCGYC 4 cut(s) 23, 128, 138, 667
Hin1II CATG 6 cut(s) 100, 157, 340, 618, 709, 757
HincII GTYRAC 2 cut(s) 136, 736
HindII GTYRAC 2 cut(s) 136, 736
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 2 cut(s) 32, 583
HpaII CCGG 3 cut(s) 45, 104, 184
HphI GGTGA 3 cut(s) 109, 839, 901
Hpy166II GTNNAC 2 cut(s) 136, 736
Hpy188I TCNGA 4 cut(s) 60, 67, 280, 684
Hpy188III TCNNGA 2 cut(s) 191, 444
Hpy8I GTNNAC 2 cut(s) 136, 736
Hpy99I CGWCG 4 cut(s) 28, 137, 140, 197
HpyAV CCTTC 6 cut(s) 142, 490, 493, 708, 724, 851
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4IV ACGT 1 cut(s) 128
HpyCH4V TGCA 2 cut(s) 506, 699
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 76, 149, 764
HpyF3I CTNAG 4 cut(s) 279, 395, 454, 681
HpySE526I ACGT 1 cut(s) 128
Hsp92I GRCGYC 4 cut(s) 23, 128, 138, 667
Hsp92II CATG 6 cut(s) 100, 157, 340, 618, 709, 757
Ksp22I TGATCA 1 cut(s) 891
Kzo9I GATC 5 cut(s) 39, 60, 499, 817, 891
LguI GCTCTTC 1 cut(s) 561
LmnI GCTCC 4 cut(s) 67, 75, 157, 799
Lsp1109I GCAGC 2 cut(s) 123, 778
MaeI CTAG 3 cut(s) 203, 653, 725
MaeII ACGT 1 cut(s) 128
MaeIII GTNAC 2 cut(s) 115, 845
MalI GATC 5 cut(s) 41, 62, 501, 819, 893
MboI GATC 5 cut(s) 39, 60, 499, 817, 891
MboII GAAGA 3 cut(s) 578, 635, 814
MflI RGATCY 2 cut(s) 60, 499
MhlI GDGCHC 2 cut(s) 72, 81
MluCI AATT 6 cut(s) 284, 327, 388, 401, 421, 775
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 2 cut(s) 9, 38
MnlI CCTC 3 cut(s) 310, 632, 704
MseI TTAA 3 cut(s) 51, 332, 747
MslI CAYNNNNRTG 1 cut(s) 704
MspI CCGG 3 cut(s) 45, 104, 184
MspR9I CCNGG 4 cut(s) 46, 104, 170, 185
Mva1269I GAATGC 1 cut(s) 581
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 4 cut(s) 14, 76, 149, 764
NciI CCSGG 3 cut(s) 46, 104, 185
NcoI CCATGG 2 cut(s) 153, 614
NdeI CATATG 1 cut(s) 763
NdeII GATC 5 cut(s) 39, 60, 499, 817, 891
NlaIII CATG 6 cut(s) 100, 157, 340, 618, 709, 757
NlaIV GGNNCC 2 cut(s) 481, 501
NmuCI GTSAC 2 cut(s) 115, 845
NruI TCGCGA 1 cut(s) 192
NspI RCATGY 1 cut(s) 100
PceI AGGCCT 1 cut(s) 833
PciI ACATGT 1 cut(s) 96
PciSI GCTCTTC 1 cut(s) 561
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PctI GAATGC 1 cut(s) 581
PfeI GAWTC 1 cut(s) 583
PkrI GCNGC 4 cut(s) 113, 142, 513, 793
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 2 cut(s) 319, 480
PscI ACATGT 1 cut(s) 96
PshAI GACNNNNGTC 1 cut(s) 131
PshBI ATTAAT 1 cut(s) 747
PsiI TTATAA 1 cut(s) 326
Psp124BI GAGCTC 2 cut(s) 72, 81
Psp5II RGGWCCY 2 cut(s) 319, 480
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 2 cut(s) 481, 501
PspPI GGNCC 5 cut(s) 166, 181, 319, 480, 838
PspPPI RGGWCCY 2 cut(s) 319, 480
PsuI RGATCY 2 cut(s) 60, 499
RruI TCGCGA 1 cut(s) 192
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
RseI CAYNNNNRTG 1 cut(s) 704
SacI GAGCTC 2 cut(s) 72, 81
SalI GTCGAC 1 cut(s) 134
SapI GCTCTTC 1 cut(s) 561
SaqAI TTAA 3 cut(s) 51, 332, 747
SatI GCNGC 4 cut(s) 112, 141, 512, 792
Sau3AI GATC 5 cut(s) 39, 60, 499, 817, 891
Sau96I GGNCC 5 cut(s) 166, 181, 319, 480, 838
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 4 cut(s) 46, 104, 170, 185
SduI GDGCHC 2 cut(s) 72, 81
SgrDI CGTCGACG 1 cut(s) 134
SinI GGWCC 5 cut(s) 166, 181, 319, 480, 838
SmiMI CAYNNNNRTG 1 cut(s) 704
SmlI CTYRAG 1 cut(s) 491
SmoI CTYRAG 1 cut(s) 491
Sse9I AATT 6 cut(s) 284, 327, 388, 401, 421, 775
SseBI AGGCCT 1 cut(s) 833
SsiI CCGC 4 cut(s) 8, 141, 509, 512
SspMI CTAG 3 cut(s) 203, 653, 725
SstI GAGCTC 2 cut(s) 72, 81
StuI AGGCCT 1 cut(s) 833
StyD4I CCNGG 4 cut(s) 44, 102, 168, 183
StyI CCWWGG 4 cut(s) 153, 614, 834, 850
TaaI ACNGT 1 cut(s) 56
TaiI ACGT 1 cut(s) 131
TaqI TCGA 2 cut(s) 135, 257
TasI AATT 6 cut(s) 284, 327, 388, 401, 421, 775
TauI GCSGC 2 cut(s) 143, 514
TfiI GAWTC 1 cut(s) 583
Tru1I TTAA 3 cut(s) 51, 332, 747
Tru9I TTAA 3 cut(s) 51, 332, 747
TseFI GTSAC 2 cut(s) 115, 845
TseI GCWGC 2 cut(s) 111, 791
Tsp45I GTSAC 2 cut(s) 115, 845
TspDTI ATGAA 1 cut(s) 306
TspGWI ACGGA 2 cut(s) 121, 886
VpaK11BI GGWCC 5 cut(s) 166, 181, 319, 480, 838
VspI ATTAAT 1 cut(s) 747
XapI RAATTY 1 cut(s) 284
XceI RCATGY 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 3 cut(s) 203, 653, 725
ZraI GACGTC 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.