RchiOBHm_Chr1g0349251

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
N/A
Physical Location & Seq
Forward (+)
42364516 .. 42366593
2078 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 486 bp
ATGGTTGATAGGTACATGAGAGGAAGGCTTACACTAGAGAAGGTGAATGCAGCTATTAATGACATGGCAACATATGCTGAAGCAAATTATCAGCTCATAGCTGCTCCCAAGAAGAATGTGGCAGGAGACCGTTGGGAAAAGGCTCTGGAAATAAGAGATATAGCGATGCATGAAGCAGTGAAGGGAAAACACTTCTTTCTTGAAACTGACATGAAAGGACCATCCTTGAAGCTTGACAACTCTGGAAAAGCAATACTAACTGTAAGGTTCTTGTCTCTCAAACAAGTATGTGTAAATTTTAAACAATATCATAGTGCACTCAGAAAGGACGAACCATTGACCGATGTATATCCCTTTTCCGTGCTAACTGCCAACTCCCTAGGCACAACTCAACCCACACACACACTCCCTGCATCATCCACCATGGCCAGATCTGAGGGTAAGCCAACTCATACCCCACTACTGATGATGTGTTGCTACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

161

Amino Acids

18.05

Weight (kDa)

9.13

Isoelectric Point (pI)

29.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 2 - 88 3.6e-34 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 1 cut(s) 295
AcuI CTGAAG 1 cut(s) 99
AfaI GTAC 1 cut(s) 14
AgsI TTSAA 2 cut(s) 203, 229
AluBI AGCT 4 cut(s) 53, 94, 101, 232
AluI AGCT 4 cut(s) 53, 94, 101, 232
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 2 cut(s) 120, 279
Alw44I GTGCAC 1 cut(s) 315
AoxI GGCC 1 cut(s) 426
ApaLI GTGCAC 1 cut(s) 315
ApeKI GCWGC 2 cut(s) 50, 101
ApoI RAATTY 1 cut(s) 295
AseI ATTAAT 1 cut(s) 57
AspA2I CCTAGG 1 cut(s) 379
AspS9I GGNCC 1 cut(s) 218
AsuHPI GGTGA 1 cut(s) 55
AvaII GGWCC 1 cut(s) 218
AvrII CCTAGG 1 cut(s) 379
BaeGI GKGCMC 1 cut(s) 319
BalI TGGCCA 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 2 cut(s) 62, 88
BccI CCATC 1 cut(s) 229
BcoDI GTCTC 2 cut(s) 120, 279
BfaI CTAG 2 cut(s) 35, 380
BglII AGATCT 1 cut(s) 431
BisI GCNGC 2 cut(s) 51, 102
BlnI CCTAGG 1 cut(s) 379
BlsI GCNGC 2 cut(s) 52, 103
Bme18I GGWCC 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 218
BmsI GCATC 2 cut(s) 156, 422
BsaBI GATNNNNATC 1 cut(s) 348
BsaI GGTCTC 1 cut(s) 120
BsaJI CCNNGG 2 cut(s) 379, 423
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bse8I GATNNNNATC 1 cut(s) 348
BseDI CCNNGG 2 cut(s) 379, 423
BseGI GGATG 2 cut(s) 221, 416
BseJI GATNNNNATC 1 cut(s) 348
BseMII CTCAG 2 cut(s) 334, 426
BseSI GKGCMC 1 cut(s) 319
BseXI GCAGC 2 cut(s) 62, 88
BshFI GGCC 1 cut(s) 428
BsiHKAI GWGCWC 1 cut(s) 319
BsmAI GTCTC 2 cut(s) 120, 279
BsmI GAATGC 1 cut(s) 52
BsnI GGCC 1 cut(s) 428
Bso31I GGTCTC 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 319
Bsp143I GATC 1 cut(s) 431
Bsp19I CCATGG 1 cut(s) 423
BspANI GGCC 1 cut(s) 428
BspCNI CTCAG 2 cut(s) 333, 427
BspTNI GGTCTC 1 cut(s) 120
BssECI CCNNGG 2 cut(s) 379, 423
BssMI GATC 1 cut(s) 431
BssT1I CCWWGG 2 cut(s) 379, 423
Bst4CI ACNGT 2 cut(s) 131, 262
BstAPI GCANNNNNTGC 1 cut(s) 74
BstDEI CTNAG 2 cut(s) 320, 435
BstDSI CCRYGG 1 cut(s) 423
BstF5I GGATG 2 cut(s) 221, 416
BstKTI GATC 1 cut(s) 434
BstMAI GTCTC 2 cut(s) 120, 279
BstMBI GATC 1 cut(s) 431
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstSLI GKGCMC 1 cut(s) 319
BstV1I GCAGC 2 cut(s) 62, 88
BstX2I RGATCY 1 cut(s) 431
BstYI RGATCY 1 cut(s) 431
BsuRI GGCC 1 cut(s) 428
BtgI CCRYGG 1 cut(s) 423
BtgZI GCGATG 1 cut(s) 179
BtsCI GGATG 2 cut(s) 221, 416
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 1 cut(s) 183
Cfr13I GGNCC 1 cut(s) 218
Csp6I GTAC 1 cut(s) 13
CviAII CATG 5 cut(s) 16, 64, 170, 211, 424
CviJI RGCY 8 cut(s) 28, 53, 94, 101, 143, 232, 428, 445
CviKI_1 RGCY 8 cut(s) 28, 53, 94, 101, 143, 232, 428, 445
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 2 cut(s) 320, 435
DpnI GATC 1 cut(s) 433
DpnII GATC 1 cut(s) 431
DraI TTTAAA 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 426
Eco130I CCWWGG 2 cut(s) 379, 423
Eco31I GGTCTC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 218
Eco57I CTGAAG 1 cut(s) 99
EcoT14I CCWWGG 2 cut(s) 379, 423
EcoT22I ATGCAT 1 cut(s) 171
ErhI CCWWGG 2 cut(s) 379, 423
FaeI CATG 5 cut(s) 19, 67, 173, 214, 427
FatI CATG 5 cut(s) 15, 63, 169, 210, 423
FauNDI CATATG 1 cut(s) 73
Fnu4HI GCNGC 2 cut(s) 51, 102
FokI GGATG 2 cut(s) 208, 403
Fsp4HI GCNGC 2 cut(s) 51, 102
FspBI CTAG 2 cut(s) 35, 380
GluI GCNGC 2 cut(s) 51, 102
HaeIII GGCC 1 cut(s) 428
Hin1II CATG 5 cut(s) 19, 67, 173, 214, 427
HindIII AAGCTT 1 cut(s) 230
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 2 cut(s) 323, 436
Hpy188III TCNNGA 3 cut(s) 146, 200, 243
Hpy8I GTNNAC 1 cut(s) 317
HpyAV CCTTC 3 cut(s) 18, 34, 175
HpyCH4III ACNGT 2 cut(s) 131, 262
HpyCH4V TGCA 4 cut(s) 50, 169, 317, 413
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 2 cut(s) 320, 435
Hsp92II CATG 5 cut(s) 19, 67, 173, 214, 427
Kzo9I GATC 1 cut(s) 431
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 5 cut(s) 108, 131, 228, 423, 442
Lsp1109I GCAGC 2 cut(s) 62, 88
LweI GCATC 2 cut(s) 156, 422
MaeI CTAG 2 cut(s) 35, 380
MalI GATC 1 cut(s) 433
MboI GATC 1 cut(s) 431
MboII GAAGA 1 cut(s) 124
MflI RGATCY 1 cut(s) 431
MhlI GDGCHC 1 cut(s) 319
MlsI TGGCCA 1 cut(s) 428
MluCI AATT 3 cut(s) 85, 295, 481
MluNI TGGCCA 1 cut(s) 428
MnlI CCTC 2 cut(s) 14, 430
Mox20I TGGCCA 1 cut(s) 428
Mph1103I ATGCAT 1 cut(s) 171
MscI TGGCCA 1 cut(s) 428
MseI TTAA 3 cut(s) 57, 300, 484
Msp20I TGGCCA 1 cut(s) 428
Mva1269I GAATGC 1 cut(s) 52
MwoI GCNNNNNNNGC 1 cut(s) 74
NcoI CCATGG 1 cut(s) 423
NdeI CATATG 1 cut(s) 73
NdeII GATC 1 cut(s) 431
NlaIII CATG 5 cut(s) 19, 67, 173, 214, 427
NsiI ATGCAT 1 cut(s) 171
PctI GAATGC 1 cut(s) 52
PkrI GCNGC 2 cut(s) 52, 103
PshBI ATTAAT 1 cut(s) 57
PspPI GGNCC 1 cut(s) 218
PsuI RGATCY 1 cut(s) 431
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
SaqAI TTAA 3 cut(s) 57, 300, 484
SatI GCNGC 2 cut(s) 51, 102
Sau3AI GATC 1 cut(s) 431
Sau96I GGNCC 1 cut(s) 218
SduI GDGCHC 1 cut(s) 319
SetI ASST 7 cut(s) 14, 45, 55, 96, 103, 234, 269
SfaNI GCATC 2 cut(s) 156, 422
SinI GGWCC 1 cut(s) 218
Sse9I AATT 3 cut(s) 85, 295, 481
SspMI CTAG 2 cut(s) 35, 380
StyI CCWWGG 2 cut(s) 379, 423
TaaI ACNGT 2 cut(s) 131, 262
TaqII GACCGA 1 cut(s) 356
TasI AATT 3 cut(s) 85, 295, 481
Tru1I TTAA 3 cut(s) 57, 300, 484
Tru9I TTAA 3 cut(s) 57, 300, 484
TscAI CASTG 1 cut(s) 183
TseI GCWGC 2 cut(s) 50, 101
TspDTI ATGAA 2 cut(s) 186, 227
TspGWI ACGGA 1 cut(s) 349
TspRI CASTG 1 cut(s) 183
VneI GTGCAC 1 cut(s) 315
VpaK11BI GGWCC 1 cut(s) 218
VspI ATTAAT 1 cut(s) 57
XapI RAATTY 1 cut(s) 295
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XmaJI CCTAGG 1 cut(s) 379
XspI CTAG 2 cut(s) 35, 380
Zsp2I ATGCAT 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.