RchiOBHm_Chr1g0371001

NADH-cytochrome b5 reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0029673
Physical Location & Seq
Forward (+)
60367341 .. 60367703
363 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 363 bp
ATGATTAGTGTTTTATATTGGAGTAATTTTAAATACACGTATCTATTTTATTACTACACACCCCAATTTTTTGTAATACTTTTTATTCCTATTTCAACATTCCAACTATTATTATCTCCAACCTTCTCTTCTGATCTAGTGATAGAGATGTATCCTCAAGGAAGAATGTCACACCATTTTAGTGAGATGTGTGTTGGAGATCATCTTGCTGTCAAGGGACCCAAGGACCGTTTCAAGTATGAGCCTGGCCAAGTTAGAACTGTTAGGATGCTTGGTAGAGCCTCTCGAATCACTCTGATGTTCCAGGTTGCTAGGGCAATATTAGAAAATCCTCTCCTAATCTATGTCAATTGTGACCTATGA

Protein Analysis

120

Amino Acids

14.14

Weight (kDa)

8.95

Isoelectric Point (pI)

32.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_6 PF00970 45 - 80 1.4e-07 Oxidoreductase FAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0029673)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0371001 RchiOBHm_Chr3g0485111

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 247
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 2 cut(s) 96, 235
AjnI CCWGG 2 cut(s) 244, 303
AjuI GAANNNNNNNTTGG 4 cut(s) 112, 144, 215, 247
AoxI GGCC 1 cut(s) 247
AspS9I GGNCC 2 cut(s) 218, 226
AvaII GGWCC 2 cut(s) 218, 226
BalI TGGCCA 1 cut(s) 249
BciT130I CCWGG 2 cut(s) 246, 305
BciVI GTATCC 1 cut(s) 162
BfaI CTAG 2 cut(s) 137, 312
BfuI GTATCC 1 cut(s) 162
Bme1390I CCNGG 2 cut(s) 246, 305
Bme18I GGWCC 2 cut(s) 218, 226
BmgT120I GGNCC 2 cut(s) 218, 226
BmiI GGNNCC 2 cut(s) 219, 220
BmrFI CCNGG 2 cut(s) 246, 305
BmsI GCATC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 141
BsaAI YACGTR 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 222
BseBI CCWGG 2 cut(s) 246, 305
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 1 cut(s) 273
BshFI GGCC 1 cut(s) 249
BslFI GGGAC 1 cut(s) 231
BsmFI GGGAC 1 cut(s) 231
BsnI GGCC 1 cut(s) 249
Bsp143I GATC 2 cut(s) 133, 199
BspANI GGCC 1 cut(s) 249
BspLI GGNNCC 2 cut(s) 219, 220
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 2 cut(s) 133, 199
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 2 cut(s) 246, 305
Bst4CI ACNGT 2 cut(s) 230, 262
Bst6I CTCTTC 1 cut(s) 133
BstBAI YACGTR 1 cut(s) 39
BstF5I GGATG 1 cut(s) 273
BstKTI GATC 2 cut(s) 136, 202
BstMBI GATC 2 cut(s) 133, 199
BstNI CCWGG 2 cut(s) 246, 305
BstSCI CCNGG 2 cut(s) 244, 303
BsuI GTATCC 1 cut(s) 162
BsuRI GGCC 1 cut(s) 249
BtsCI GGATG 1 cut(s) 273
Cfr13I GGNCC 2 cut(s) 218, 226
CviJI RGCY 3 cut(s) 244, 249, 281
CviKI_1 RGCY 3 cut(s) 244, 249, 281
DpnI GATC 2 cut(s) 135, 201
DpnII GATC 2 cut(s) 133, 199
DraI TTTAAA 1 cut(s) 31
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 1 cut(s) 133
EarI CTCTTC 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 222
Eco47I GGWCC 2 cut(s) 218, 226
EcoO109I RGGNCCY 1 cut(s) 218
EcoRII CCWGG 2 cut(s) 244, 303
EcoT14I CCWWGG 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 222
FaiI YATR 4 cut(s) 16, 240, 345, 361
FaqI GGGAC 1 cut(s) 231
FokI GGATG 1 cut(s) 280
FspBI CTAG 2 cut(s) 137, 312
HaeIII GGCC 1 cut(s) 249
HinfI GANTC 1 cut(s) 288
Hpy188I TCNGA 2 cut(s) 133, 297
Hpy188III TCNNGA 1 cut(s) 285
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 2 cut(s) 230, 262
HpyCH4IV ACGT 1 cut(s) 38
HpySE526I ACGT 1 cut(s) 38
KflI GGGWCCC 1 cut(s) 218
Kzo9I GATC 2 cut(s) 133, 199
LpnPI CCDG 4 cut(s) 231, 258, 290, 317
LweI GCATC 1 cut(s) 258
MaeI CTAG 2 cut(s) 137, 312
MaeII ACGT 1 cut(s) 38
MaeIII GTNAC 2 cut(s) 168, 353
MalI GATC 2 cut(s) 135, 201
MboI GATC 2 cut(s) 133, 199
MboII GAAGA 2 cut(s) 120, 174
MfeI CAATTG 1 cut(s) 349
MlsI TGGCCA 1 cut(s) 249
MluCI AATT 3 cut(s) 25, 65, 349
MluNI TGGCCA 1 cut(s) 249
MmeI TCCRAC 3 cut(s) 127, 143, 175
MnlI CCTC 3 cut(s) 165, 292, 342
Mox20I TGGCCA 1 cut(s) 249
MscI TGGCCA 1 cut(s) 249
MseI TTAA 1 cut(s) 30
MslI CAYNNNNRTG 2 cut(s) 180, 296
Msp20I TGGCCA 1 cut(s) 249
MspR9I CCNGG 2 cut(s) 246, 305
MunI CAATTG 1 cut(s) 349
MvaI CCWGG 2 cut(s) 246, 305
NdeII GATC 2 cut(s) 133, 199
NlaIV GGNNCC 2 cut(s) 219, 220
NmuCI GTSAC 2 cut(s) 168, 353
PfeI GAWTC 1 cut(s) 288
Ppu21I YACGTR 1 cut(s) 39
PpuMI RGGWCCY 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 218
Psp6I CCWGG 2 cut(s) 244, 303
PspGI CCWGG 2 cut(s) 244, 303
PspN4I GGNNCC 2 cut(s) 219, 220
PspPI GGNCC 2 cut(s) 218, 226
PspPPI RGGWCCY 1 cut(s) 218
RseI CAYNNNNRTG 2 cut(s) 180, 296
SaqAI TTAA 1 cut(s) 30
Sau3AI GATC 2 cut(s) 133, 199
Sau96I GGNCC 2 cut(s) 218, 226
ScrFI CCNGG 2 cut(s) 246, 305
SetI ASST 4 cut(s) 41, 125, 309, 360
SfaNI GCATC 1 cut(s) 258
SinI GGWCC 2 cut(s) 218, 226
SmiMI CAYNNNNRTG 2 cut(s) 180, 296
SmlI CTYRAG 1 cut(s) 156
SmoI CTYRAG 1 cut(s) 156
Sse9I AATT 3 cut(s) 25, 65, 349
SspI AATATT 1 cut(s) 321
SspMI CTAG 2 cut(s) 137, 312
StyD4I CCNGG 2 cut(s) 244, 303
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 2 cut(s) 230, 262
TaiI ACGT 1 cut(s) 41
TaqI TCGA 1 cut(s) 286
TasI AATT 3 cut(s) 25, 65, 349
TfiI GAWTC 1 cut(s) 288
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TseFI GTSAC 2 cut(s) 168, 353
Tsp45I GTSAC 2 cut(s) 168, 353
VpaK11BI GGWCC 2 cut(s) 218, 226
XspI CTAG 2 cut(s) 137, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.