RchiOBHm_Chr2g0121471

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
N/A
Physical Location & Seq
Forward (+)
34334414 .. 34337082
2669 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49398

Sequence Viewer

Length: 1224 bp
ATGTATGGAACCTTTGATAATGAACAAGAAATAGTAGTGAAGAGACTGTCAAAAAGTTCCGGCCAAGGTGTACGAGAATTTAAGAATGAGGTTCTATTGGTTGCCAAGCTTCAACATCGAAATCTTGTCAGGCTTCTGGGATTTTGCTTGGAAGGAGAAGAGACCATACTTGTCTATGAATATGTACCCAACAAAAGTCTTGATCATTTTCTTTTTGAGAGAGACACACACACACACATATATATATATATATACACACCGGAAGGTTCTGAAGAGGACGTCCGCAACCCAGTAATAGTGCAGACGTCCCTCCTCCGGCGTCGGGAAGGCGGTGATCGCAGCGTTGCGGGTGCCAGACGCACTCTCTGGACATCCCAGACAGCCTCGATCAAGCTGCAAAGGCGAAGACGATCAGAAACTCGATGGTGTGCAGCGAGCTCGCCAGAAACCATGGAAGCCCACAAATCTGCAACCCCGACCTCGATTTCTGCCCAGAAGAGGTCTGGGGAGCCTCTGGACTCTATCCGGCCAGCCCCGTGCCGCCGTCGCCTCCTGAAAACTCCTGCTACGTCGACGTCCGCACTTTTTCTGGGTTGCGAACGTCCCCTTCAGATCCTTCCCGTATATAGACACACACACACACACACACACTATAATAATAAACCCATATATGTATGTTTGATGAATACGAGGGTAGAATCCAAAGGACGAGAACAGCTAGAGTGGTTGAGACGTAACTCGATTATAGGAGGGATCGCTGGAGGACTTGTATATCTTCATGAAGATTCTAGGCTTAGAGTCATTCATTGTGATTTGAAAGCTAGCAATATCCTGCTAGATGCCAATATGAATCCTAAAATTTCAGATTTTGGGATGGCAAGAATGTTTGGAATTGATGATCAAACTCAAGGAAGCACCAAAAGAATTGTCGCTACATGGCTCCAGGTGATGGAGGGATTGTATTCTATAAAGTCTAACGTCTTCAGCTTCAGAATTCTCTTGCTAGAGATTATAACTGGAAGAAATTTGTTTTTAGGGTTTCATCATACAAACTATCCACCAACGCTCCTCAGTTATGCTTGGAAATTATGGAATGAGGGGAAAGTGTTGGAGTTGATGGATCCATTGCTAAAAGATTATGGCAGTTCAAATGAGTTCTTGAGATACATTCACATCAGGTTATTGTGTGTTCAAGAAGACGAAAACAAAAGGCCAACCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3403 Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

407

Amino Acids

46.89

Weight (kDa)

9.34

Isoelectric Point (pI)

59.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 6 - 343 2.5e-29 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 6 - 75 3.8e-07 Protein kinase domain
Pkinase PF00069 245 - 351 2.6e-15 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1013
AatII GACGTC 3 cut(s) 282, 308, 578
AccB1I GGYRCC 1 cut(s) 350
AccB7I CCANNNNNTGG 1 cut(s) 949
AccI GTMKAC 1 cut(s) 572
AciI CCGC 5 cut(s) 283, 330, 347, 541, 579
AclWI GGATC 4 cut(s) 607, 761, 1115, 1128
AcoI YGGCCR 2 cut(s) 61, 527
AcsI RAATTY 4 cut(s) 77, 858, 993, 1024
AcuI CTGAAG 4 cut(s) 291, 593, 967, 973
AcyI GRCGYC 4 cut(s) 279, 305, 319, 575
AfaI GTAC 2 cut(s) 72, 186
AfiI CCNNNNNNNGG 4 cut(s) 315, 322, 498, 949
AgsI TTSAA 4 cut(s) 113, 817, 1149, 1193
AjnI CCWGG 1 cut(s) 942
AluBI AGCT 6 cut(s) 109, 394, 438, 718, 821, 987
AluI AGCT 6 cut(s) 109, 394, 438, 718, 821, 987
Alw21I GWGCWC 1 cut(s) 440
Alw26I GTCTC 4 cut(s) 37, 155, 216, 724
AlwI GGATC 4 cut(s) 607, 761, 1115, 1128
AoxI GGCC 3 cut(s) 61, 527, 1211
ApeKI GCWGC 3 cut(s) 339, 394, 431
ApoI RAATTY 4 cut(s) 77, 858, 993, 1024
ArsI GACNNNNNNTTYG 2 cut(s) 912, 944
AsuHPI GGTGA 2 cut(s) 344, 958
AsuNHI GCTAGC 1 cut(s) 821
BamHI GGATCC 1 cut(s) 1120
BanI GGYRCC 1 cut(s) 350
BanII GRGCYC 1 cut(s) 440
BarI GAAGNNNNNNTAC 2 cut(s) 150, 182
BbsI GAAGAC 3 cut(s) 412, 973, 1203
Bbv12I GWGCWC 1 cut(s) 440
BbvI GCAGC 3 cut(s) 351, 381, 443
BccI CCATC 4 cut(s) 417, 868, 943, 1111
BceAI ACGGC 1 cut(s) 528
BcgI CGANNNNNNTGC 4 cut(s) 376, 410, 420, 454
BciT130I CCWGG 1 cut(s) 944
BclI TGATCA 2 cut(s) 202, 898
BcoDI GTCTC 4 cut(s) 37, 155, 216, 724
BfaI CTAG 5 cut(s) 719, 789, 822, 836, 1004
BisI GCNGC 4 cut(s) 340, 395, 432, 541
BlsI GCNGC 4 cut(s) 341, 396, 433, 542
Bme1390I CCNGG 1 cut(s) 944
BmiI GGNNCC 5 cut(s) 10, 352, 510, 941, 1122
BmrFI CCNGG 1 cut(s) 944
BmrI ACTGGG 1 cut(s) 284
BmsI GCATC 1 cut(s) 829
BmtI GCTAGC 1 cut(s) 825
BmuI ACTGGG 1 cut(s) 284
BpiI GAAGAC 3 cut(s) 412, 973, 1203
BpmI CTGGAG 2 cut(s) 780, 926
BpuEI CTTGAG 2 cut(s) 891, 1180
BsaHI GRCGYC 4 cut(s) 279, 305, 319, 575
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 64, 450
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 6 cut(s) 753, 783, 944, 974, 1050, 1080
Bsc4I CCNNNNNNNGG 4 cut(s) 315, 322, 498, 949
Bse1I ACTGG 2 cut(s) 290, 1021
Bse3DI GCAATG 1 cut(s) 1124
BseBI CCWGG 1 cut(s) 944
BseDI CCNNGG 2 cut(s) 64, 450
BseGI GGATG 2 cut(s) 371, 879
BseLI CCNNNNNNNGG 4 cut(s) 315, 322, 498, 949
BseMI GCAATG 1 cut(s) 1124
BseMII CTCAG 1 cut(s) 1084
BseNI ACTGG 2 cut(s) 290, 1021
BseRI GAGGAG 2 cut(s) 302, 1058
BseXI GCAGC 3 cut(s) 351, 381, 443
BsgI GTGCAG 2 cut(s) 320, 450
BshFI GGCC 3 cut(s) 63, 529, 1213
BshNI GGYRCC 1 cut(s) 350
BsiHKAI GWGCWC 1 cut(s) 440
BsiSI CCGG 4 cut(s) 60, 260, 316, 526
BslFI GGGAC 2 cut(s) 292, 588
BslI CCNNNNNNNGG 4 cut(s) 315, 322, 498, 949
BsmAI GTCTC 4 cut(s) 37, 155, 216, 724
BsmBI CGTCTC 1 cut(s) 724
BsmFI GGGAC 2 cut(s) 292, 588
BsnI GGCC 3 cut(s) 63, 529, 1213
Bso31I GGTCTC 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 440
Bsp143I GATC 8 cut(s) 202, 334, 387, 410, 612, 753, 898, 1120
Bsp19I CCATGG 1 cut(s) 450
BspACI CCGC 5 cut(s) 283, 330, 347, 541, 579
BspANI GGCC 3 cut(s) 63, 529, 1213
BspCNI CTCAG 1 cut(s) 1083
BspHI TCATGA 1 cut(s) 778
BspLI GGNNCC 5 cut(s) 10, 352, 510, 941, 1122
BspOI GCTAGC 1 cut(s) 825
BspPI GGATC 4 cut(s) 607, 761, 1115, 1128
BspT107I GGYRCC 1 cut(s) 350
BspTNI GGTCTC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 1124
BsrI ACTGG 2 cut(s) 290, 1021
BssECI CCNNGG 2 cut(s) 64, 450
BssMI GATC 8 cut(s) 202, 334, 387, 410, 612, 753, 898, 1120
BssNI GRCGYC 4 cut(s) 279, 305, 319, 575
BssT1I CCWWGG 2 cut(s) 64, 450
Bst2UI CCWGG 1 cut(s) 944
Bst4CI ACNGT 1 cut(s) 48
Bst6I CTCTTC 4 cut(s) 35, 153, 267, 491
BstACI GRCGYC 4 cut(s) 279, 305, 319, 575
BstC8I GCNNGC 4 cut(s) 436, 440, 531, 823
BstDEI CTNAG 2 cut(s) 794, 1070
BstDSI CCRYGG 1 cut(s) 450
BstF5I GGATG 2 cut(s) 371, 879
BstKTI GATC 8 cut(s) 205, 337, 390, 413, 615, 756, 901, 1123
BstMAI GTCTC 4 cut(s) 37, 155, 216, 724
BstMBI GATC 8 cut(s) 202, 334, 387, 410, 612, 753, 898, 1120
BstMWI GCNNNNNNNGC 3 cut(s) 336, 400, 546
BstNI CCWGG 1 cut(s) 944
BstSCI CCNGG 1 cut(s) 942
BstV1I GCAGC 3 cut(s) 351, 381, 443
BstV2I GAAGAC 3 cut(s) 412, 973, 1203
BstX2I RGATCY 2 cut(s) 612, 1120
BstYI RGATCY 2 cut(s) 612, 1120
BsuRI GGCC 3 cut(s) 63, 529, 1213
BtgI CCRYGG 1 cut(s) 450
BtsCI GGATG 2 cut(s) 371, 879
Cac8I GCNNGC 4 cut(s) 436, 440, 531, 823
CciI TCATGA 1 cut(s) 778
CseI GACGC 2 cut(s) 308, 366
Csp6I GTAC 2 cut(s) 71, 185
CviAII CATG 4 cut(s) 451, 779, 936, 1219
CviQI GTAC 2 cut(s) 71, 185
DdeI CTNAG 2 cut(s) 794, 1070
DpnI GATC 8 cut(s) 204, 336, 389, 412, 614, 755, 900, 1122
DpnII GATC 8 cut(s) 202, 334, 387, 410, 612, 753, 898, 1120
EaeI YGGCCR 2 cut(s) 61, 527
Eam1104I CTCTTC 4 cut(s) 35, 153, 267, 491
EarI CTCTTC 4 cut(s) 35, 153, 267, 491
Ecl136II GAGCTC 1 cut(s) 438
Eco130I CCWWGG 2 cut(s) 64, 450
Eco24I GRGCYC 1 cut(s) 440
Eco31I GGTCTC 1 cut(s) 155
Eco53kI GAGCTC 1 cut(s) 438
Eco57I CTGAAG 4 cut(s) 291, 593, 967, 973
EcoICRI GAGCTC 1 cut(s) 438
EcoRI GAATTC 1 cut(s) 993
EcoRII CCWGG 1 cut(s) 942
EcoT14I CCWWGG 2 cut(s) 64, 450
EcoT38I GRGCYC 1 cut(s) 440
ErhI CCWWGG 2 cut(s) 64, 450
Esp3I CGTCTC 1 cut(s) 724
FaeI CATG 4 cut(s) 454, 782, 939, 1222
FaqI GGGAC 2 cut(s) 292, 588
FatI CATG 4 cut(s) 450, 778, 935, 1218
FauI CCCGC 1 cut(s) 340
FbaI TGATCA 2 cut(s) 202, 898
FblI GTMKAC 1 cut(s) 572
Fnu4HI GCNGC 4 cut(s) 340, 395, 432, 541
FokI GGATG 2 cut(s) 358, 886
FriOI GRGCYC 1 cut(s) 440
Fsp4HI GCNGC 4 cut(s) 340, 395, 432, 541
FspBI CTAG 5 cut(s) 719, 789, 822, 836, 1004
GluI GCNGC 4 cut(s) 340, 395, 432, 541
GsuI CTGGAG 2 cut(s) 780, 926
HaeIII GGCC 3 cut(s) 63, 529, 1213
HapII CCGG 4 cut(s) 60, 260, 316, 526
HgaI GACGC 2 cut(s) 308, 366
Hin1I GRCGYC 4 cut(s) 279, 305, 319, 575
Hin1II CATG 4 cut(s) 454, 782, 939, 1222
HincII GTYRAC 1 cut(s) 573
HindII GTYRAC 1 cut(s) 573
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 5 cut(s) 518, 698, 785, 798, 850
HpaII CCGG 4 cut(s) 60, 260, 316, 526
HphI GGTGA 2 cut(s) 344, 958
Hpy166II GTNNAC 2 cut(s) 71, 573
Hpy188I TCNGA 5 cut(s) 271, 415, 612, 865, 992
Hpy188III TCNNGA 8 cut(s) 200, 323, 367, 515, 553, 779, 1159, 1193
Hpy8I GTNNAC 2 cut(s) 71, 573
Hpy99I CGWCG 4 cut(s) 324, 549, 574, 577
HpyAV CCTTC 5 cut(s) 146, 257, 320, 617, 626
HpyCH4III ACNGT 1 cut(s) 48
HpyCH4IV ACGT 7 cut(s) 279, 305, 569, 575, 601, 733, 978
HpyCH4V TGCA 4 cut(s) 301, 397, 431, 470
HpyF10VI GCNNNNNNNGC 3 cut(s) 336, 400, 546
HpyF3I CTNAG 2 cut(s) 794, 1070
HpySE526I ACGT 7 cut(s) 279, 305, 569, 575, 601, 733, 978
Hsp92I GRCGYC 4 cut(s) 279, 305, 319, 575
Hsp92II CATG 4 cut(s) 454, 782, 939, 1222
Ksp22I TGATCA 2 cut(s) 202, 898
Kzo9I GATC 8 cut(s) 202, 334, 387, 410, 612, 753, 898, 1120
LmnI GCTCC 3 cut(s) 508, 945, 1071
Lsp1109I GCAGC 3 cut(s) 351, 381, 443
LweI GCATC 1 cut(s) 829
MaeI CTAG 5 cut(s) 719, 789, 822, 836, 1004
MaeII ACGT 7 cut(s) 279, 305, 569, 575, 601, 733, 978
MaeIII GTNAC 1 cut(s) 734
MalI GATC 8 cut(s) 204, 336, 389, 412, 614, 755, 900, 1122
MboI GATC 8 cut(s) 202, 334, 387, 410, 612, 753, 898, 1120
MflI RGATCY 2 cut(s) 612, 1120
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 7 cut(s) 77, 858, 891, 924, 993, 1024, 1085
MlyI GAGTC 2 cut(s) 512, 807
MmeI TCCRAC 1 cut(s) 1089
MseI TTAA 1 cut(s) 81
MspI CCGG 4 cut(s) 60, 260, 316, 526
MspR9I CCNGG 1 cut(s) 944
MvaI CCWGG 1 cut(s) 944
MwoI GCNNNNNNNGC 3 cut(s) 336, 400, 546
NcoI CCATGG 1 cut(s) 450
NdeII GATC 8 cut(s) 202, 334, 387, 410, 612, 753, 898, 1120
NheI GCTAGC 1 cut(s) 821
NlaIII CATG 4 cut(s) 454, 782, 939, 1222
NlaIV GGNNCC 5 cut(s) 10, 352, 510, 941, 1122
PagI TCATGA 1 cut(s) 778
PfeI GAWTC 3 cut(s) 698, 785, 850
PflMI CCANNNNNTGG 1 cut(s) 949
PkrI GCNGC 4 cut(s) 341, 396, 433, 542
PleI GAGTC 2 cut(s) 512, 806
PpsI GAGTC 2 cut(s) 512, 806
PsiI TTATAA 1 cut(s) 1013
Psp124BI GAGCTC 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 942
PspGI CCWGG 1 cut(s) 942
PspN4I GGNNCC 5 cut(s) 10, 352, 510, 941, 1122
PsuI RGATCY 2 cut(s) 612, 1120
RsaI GTAC 2 cut(s) 72, 186
RsaNI GTAC 2 cut(s) 71, 185
SacI GAGCTC 1 cut(s) 440
SalI GTCGAC 1 cut(s) 571
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 4 cut(s) 340, 395, 432, 541
Sau3AI GATC 8 cut(s) 202, 334, 387, 410, 612, 753, 898, 1120
SchI GAGTC 2 cut(s) 512, 807
ScrFI CCNGG 1 cut(s) 944
SduI GDGCHC 1 cut(s) 440
SfaNI GCATC 1 cut(s) 829
SgrDI CGTCGACG 1 cut(s) 571
SmlI CTYRAG 2 cut(s) 906, 1159
SmoI CTYRAG 2 cut(s) 906, 1159
Sse9I AATT 7 cut(s) 77, 858, 891, 924, 993, 1024, 1085
SsiI CCGC 5 cut(s) 283, 330, 347, 541, 579
SspMI CTAG 5 cut(s) 719, 789, 822, 836, 1004
SstI GAGCTC 1 cut(s) 440
StyD4I CCNGG 1 cut(s) 942
StyI CCWWGG 2 cut(s) 64, 450
TaaI ACNGT 1 cut(s) 48
TaiI ACGT 7 cut(s) 282, 308, 572, 578, 604, 736, 981
TaqI TCGA 6 cut(s) 118, 386, 421, 482, 572, 740
TasI AATT 7 cut(s) 77, 858, 891, 924, 993, 1024, 1085
TauI GCSGC 1 cut(s) 543
TfiI GAWTC 3 cut(s) 698, 785, 850
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TseI GCWGC 3 cut(s) 339, 394, 431
TspDTI ATGAA 8 cut(s) 36, 192, 698, 767, 794, 795, 863, 1031
Van91I CCANNNNNTGG 1 cut(s) 949
XapI RAATTY 4 cut(s) 77, 858, 993, 1024
XcmI CCANNNNNNNNNTGG 1 cut(s) 500
XmiI GTMKAC 1 cut(s) 572
XspI CTAG 5 cut(s) 719, 789, 822, 836, 1004
ZraI GACGTC 3 cut(s) 280, 306, 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.