RchiOBHm_Chr2g0150901

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
N/A
Physical Location & Seq
Forward (+)
68566714 .. 68567986
1273 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52015

Sequence Viewer

Length: 522 bp
ATGGACCGCCAGGACTCCGTCACGGCCCGCTTCACTCTCGGCAGGCAGTCCTCGCTCGCCCCCGACCCGGACGACGGCTCGGTGGTCGAGCCGGCCGACGGGGCCCTTGACCCGAGGGTCCGACTCATGTACTTGGCCAACGAGGGCGACTTGGAAGGCATTCAGGAGCTCCTGGACTCCGGAACCAATGTCAATTTCACTGACATAGATGGACGCACCGCTCTCCACGTCGCCGCCTGTCAGGGCCGGACCGACGTCGTTCAGTTGCTCCTCCAAAGAGGCGCCGGCGTCAACCTCCAGGACCGCTGGGGCAGTACGCCTCTTGCGGATGCGGTGTACTACAAGAATGAGGATGTGATCAAGCTTTTAGAGCAGCACGGAGCTAAACTTCCGATGGCTCCTATGCATGTGGAGAATGCACGTGAAGTCCCCGAGTATGAGATTGATCCCAAGGAGCTTGATTTTTCAGACAGCGTTGACATAACCAAGGTAATGTTTTTAGTTTGGTGCAAGACGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Pkinase_Tyr

Protein Analysis

173

Amino Acids

19.12

Weight (kDa)

4.45

Isoelectric Point (pI)

34.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 42 - 129 5.8e-19 Ankyrin repeats (3 copies)
Ank_4 PF13637 42 - 91 3.9e-09 Ankyrin repeats (many copies)
Ank_5 PF13857 63 - 108 1.3e-08 Ankyrin repeats (many copies)
Ank PF00023 70 - 102 5.6e-10 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
AatII GACGTC 1 cut(s) 258
AccB1I GGYRCC 1 cut(s) 281
AccBSI CCGCTC 1 cut(s) 221
AccIII TCCGGA 1 cut(s) 179
AciI CCGC 7 cut(s) 7, 28, 219, 234, 304, 326, 332
AclWI GGATC 1 cut(s) 440
AcoI YGGCCR 2 cut(s) 93, 135
AcvI CACGTG 1 cut(s) 422
AcyI GRCGYC 3 cut(s) 255, 282, 288
AfaI GTAC 3 cut(s) 131, 316, 338
AfiI CCNNNNNNNGG 3 cut(s) 67, 74, 98
AjiI CACGTC 1 cut(s) 229
AjnI CCWGG 3 cut(s) 9, 171, 297
AjuI GAANNNNNNNTTGG 2 cut(s) 179, 211
AluBI AGCT 4 cut(s) 169, 364, 383, 457
AluI AGCT 4 cut(s) 169, 364, 383, 457
Alw21I GWGCWC 1 cut(s) 171
AlwI GGATC 1 cut(s) 440
Ama87I CYCGRG 2 cut(s) 112, 431
Aor13HI TCCGGA 1 cut(s) 179
AoxI GGCC 5 cut(s) 24, 93, 102, 135, 244
ApaI GGGCCC 1 cut(s) 106
ApeKI GCWGC 1 cut(s) 373
Asp700I GAANNNNTTC 1 cut(s) 159
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 8 cut(s) 4, 25, 102, 103, 118, 244, 249, 301
AsuC2I CCSGG 1 cut(s) 68
AvaI CYCGRG 2 cut(s) 112, 431
AvaII GGWCC 4 cut(s) 4, 118, 249, 301
BaeGI GKGCMC 1 cut(s) 106
BalI TGGCCA 1 cut(s) 137
BanI GGYRCC 1 cut(s) 281
BanII GRGCYC 2 cut(s) 106, 171
BbrPI CACGTG 1 cut(s) 422
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 1 cut(s) 385
BccI CCATC 2 cut(s) 203, 388
BceAI ACGGC 2 cut(s) 39, 91
BciT130I CCWGG 3 cut(s) 11, 173, 299
BclI TGATCA 1 cut(s) 357
BcnI CCSGG 1 cut(s) 68
BfoI RGCGCY 1 cut(s) 285
BglI GCCNNNNNGGC 1 cut(s) 101
BisI GCNGC 2 cut(s) 234, 374
BlsI GCNGC 2 cut(s) 235, 375
Bme1390I CCNGG 4 cut(s) 11, 68, 173, 299
Bme18I GGWCC 4 cut(s) 4, 118, 249, 301
BmeT110I CYCGRG 2 cut(s) 112, 431
BmgBI CACGTC 1 cut(s) 229
BmgT120I GGNCC 8 cut(s) 4, 25, 102, 103, 118, 244, 249, 301
BmiI GGNNCC 6 cut(s) 103, 104, 119, 184, 283, 399
BmrFI CCNGG 4 cut(s) 11, 68, 173, 299
BmsI GCATC 1 cut(s) 319
BoxI GACNNNNGTC 1 cut(s) 254
BpmI CTGGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 68
BsaAI YACGTR 1 cut(s) 422
BsaHI GRCGYC 3 cut(s) 255, 282, 288
BsaJI CCNNGG 3 cut(s) 113, 450, 486
BsaWI WCCGGW 1 cut(s) 179
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 74, 98
Bse118I RCCGGY 2 cut(s) 91, 284
BseAI TCCGGA 1 cut(s) 179
BseBI CCWGG 3 cut(s) 11, 173, 299
BseDI CCNNGG 3 cut(s) 113, 450, 486
BseGI GGATG 2 cut(s) 334, 358
BseLI CCNNNNNNNGG 3 cut(s) 67, 74, 98
BseRI GAGGAG 1 cut(s) 260
BseSI GKGCMC 1 cut(s) 106
BseX3I CGGCCG 1 cut(s) 93
BseXI GCAGC 1 cut(s) 385
BseYI CCCAGC 1 cut(s) 306
Bsh1285I CGRYCG 1 cut(s) 96
BshFI GGCC 5 cut(s) 26, 95, 104, 137, 246
BshNI GGYRCC 1 cut(s) 281
BsiEI CGRYCG 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 171
BsiHKCI CYCGRG 2 cut(s) 112, 431
BsiSI CCGG 5 cut(s) 68, 92, 180, 247, 285
BslFI GGGAC 1 cut(s) 413
BslI CCNNNNNNNGG 3 cut(s) 67, 74, 98
BsmFI GGGAC 1 cut(s) 413
BsmI GAATGC 2 cut(s) 159, 421
BsnI GGCC 5 cut(s) 26, 95, 104, 137, 246
BsoBI CYCGRG 2 cut(s) 112, 431
Bsp120I GGGCCC 1 cut(s) 102
Bsp1286I GDGCHC 2 cut(s) 106, 171
Bsp13I TCCGGA 1 cut(s) 179
Bsp143I GATC 2 cut(s) 357, 445
BspACI CCGC 7 cut(s) 7, 28, 219, 234, 304, 326, 332
BspANI GGCC 5 cut(s) 26, 95, 104, 137, 246
BspEI TCCGGA 1 cut(s) 179
BspLI GGNNCC 6 cut(s) 103, 104, 119, 184, 283, 399
BspPI GGATC 1 cut(s) 440
BspT107I GGYRCC 1 cut(s) 281
BsrBI CCGCTC 1 cut(s) 221
BsrFI RCCGGY 2 cut(s) 91, 284
BssAI RCCGGY 2 cut(s) 91, 284
BssECI CCNNGG 3 cut(s) 113, 450, 486
BssMI GATC 2 cut(s) 357, 445
BssNI GRCGYC 3 cut(s) 255, 282, 288
BssT1I CCWWGG 2 cut(s) 450, 486
Bst2UI CCWGG 3 cut(s) 11, 173, 299
BstACI GRCGYC 3 cut(s) 255, 282, 288
BstBAI YACGTR 1 cut(s) 422
BstC8I GCNNGC 5 cut(s) 28, 44, 57, 93, 286
BstF5I GGATG 2 cut(s) 334, 358
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 2 cut(s) 360, 448
BstMBI GATC 2 cut(s) 357, 445
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 3 cut(s) 52, 101, 370
BstNI CCWGG 3 cut(s) 11, 173, 299
BstNSI RCATGY 1 cut(s) 410
BstPAI GACNNNNGTC 1 cut(s) 254
BstSCI CCNGG 4 cut(s) 9, 66, 171, 297
BstSLI GKGCMC 1 cut(s) 106
BstV1I GCAGC 1 cut(s) 385
BstZI CGGCCG 1 cut(s) 93
BsuRI GGCC 5 cut(s) 26, 95, 104, 137, 246
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 2 cut(s) 334, 358
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 5 cut(s) 28, 44, 57, 93, 286
CfoI GCGC 1 cut(s) 284
Cfr10I RCCGGY 2 cut(s) 91, 284
Cfr13I GGNCC 8 cut(s) 4, 25, 102, 103, 118, 244, 249, 301
CpoI CGGWCCG 1 cut(s) 249
CseI GACGC 2 cut(s) 222, 277
Csp6I GTAC 3 cut(s) 130, 315, 337
CspI CGGWCCG 1 cut(s) 249
CviAII CATG 2 cut(s) 127, 407
CviQI GTAC 3 cut(s) 130, 315, 337
DinI GGCGCC 1 cut(s) 283
DpnI GATC 2 cut(s) 359, 447
DpnII GATC 2 cut(s) 357, 445
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
EaeI YGGCCR 2 cut(s) 93, 135
EagI CGGCCG 1 cut(s) 93
Ecl136II GAGCTC 1 cut(s) 169
EclXI CGGCCG 1 cut(s) 93
Eco130I CCWWGG 2 cut(s) 450, 486
Eco24I GRGCYC 2 cut(s) 106, 171
Eco47I GGWCC 4 cut(s) 4, 118, 249, 301
Eco52I CGGCCG 1 cut(s) 93
Eco53kI GAGCTC 1 cut(s) 169
Eco72I CACGTG 1 cut(s) 422
Eco88I CYCGRG 2 cut(s) 112, 431
EcoICRI GAGCTC 1 cut(s) 169
EcoO109I RGGNCCY 2 cut(s) 102, 103
EcoRII CCWGG 3 cut(s) 9, 171, 297
EcoT14I CCWWGG 2 cut(s) 450, 486
EcoT22I ATGCAT 1 cut(s) 408
EcoT38I GRGCYC 2 cut(s) 106, 171
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
ErhI CCWWGG 2 cut(s) 450, 486
FaeI CATG 2 cut(s) 130, 410
FaiI YATR 6 cut(s) 128, 206, 404, 408, 438, 482
FaqI GGGAC 1 cut(s) 413
FatI CATG 2 cut(s) 126, 406
FauI CCCGC 1 cut(s) 35
FbaI TGATCA 1 cut(s) 357
Fnu4HI GCNGC 2 cut(s) 234, 374
FokI GGATG 2 cut(s) 341, 365
FriOI GRGCYC 2 cut(s) 106, 171
Fsp4HI GCNGC 2 cut(s) 234, 374
GlaI GCGC 1 cut(s) 283
GluI GCNGC 2 cut(s) 234, 374
GsaI CCCAGC 1 cut(s) 310
GsuI CTGGAG 1 cut(s) 281
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 5 cut(s) 26, 95, 104, 137, 246
HapII CCGG 5 cut(s) 68, 92, 180, 247, 285
HgaI GACGC 2 cut(s) 222, 277
HhaI GCGC 1 cut(s) 284
Hin1I GRCGYC 3 cut(s) 255, 282, 288
Hin1II CATG 2 cut(s) 130, 410
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HincII GTYRAC 2 cut(s) 292, 478
HindII GTYRAC 2 cut(s) 292, 478
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 3 cut(s) 14, 123, 176
HpaII CCGG 5 cut(s) 68, 92, 180, 247, 285
Hpy166II GTNNAC 3 cut(s) 292, 337, 478
Hpy188I TCNGA 3 cut(s) 122, 393, 469
Hpy188III TCNNGA 2 cut(s) 164, 180
Hpy8I GTNNAC 3 cut(s) 292, 337, 478
Hpy99I CGWCG 5 cut(s) 77, 101, 233, 257, 260
HpyAV CCTTC 1 cut(s) 149
HpyCH4IV ACGT 3 cut(s) 228, 255, 421
HpyCH4V TGCA 3 cut(s) 406, 419, 510
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 101, 370
HpySE526I ACGT 3 cut(s) 228, 255, 421
Hsp92I GRCGYC 3 cut(s) 255, 282, 288
Hsp92II CATG 2 cut(s) 130, 410
HspAI GCGC 1 cut(s) 282
KasI GGCGCC 1 cut(s) 281
Kpn2I TCCGGA 1 cut(s) 179
KroI GCCGGC 2 cut(s) 91, 284
KroNI GCCGGC 2 cut(s) 93, 286
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 2 cut(s) 357, 445
LmnI GCTCC 6 cut(s) 166, 174, 273, 380, 403, 454
Lsp1109I GCAGC 1 cut(s) 385
LweI GCATC 1 cut(s) 319
MaeII ACGT 3 cut(s) 228, 255, 421
MaeIII GTNAC 1 cut(s) 19
MalI GATC 2 cut(s) 359, 447
MbiI CCGCTC 1 cut(s) 221
MboI GATC 2 cut(s) 357, 445
MhlI GDGCHC 2 cut(s) 106, 171
MlsI TGGCCA 1 cut(s) 137
MluCI AATT 1 cut(s) 193
MluNI TGGCCA 1 cut(s) 137
Mly113I GGCGCC 1 cut(s) 282
MlyI GAGTC 3 cut(s) 8, 117, 170
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 8 cut(s) 61, 108, 136, 272, 281, 305, 330, 343
Mox20I TGGCCA 1 cut(s) 137
Mph1103I ATGCAT 1 cut(s) 408
MreI CGCCGGCG 1 cut(s) 284
MroI TCCGGA 1 cut(s) 179
MroNI GCCGGC 2 cut(s) 91, 284
MroXI GAANNNNTTC 1 cut(s) 159
MscI TGGCCA 1 cut(s) 137
Msp20I TGGCCA 1 cut(s) 137
MspA1I CMGCKG 1 cut(s) 306
MspI CCGG 5 cut(s) 68, 92, 180, 247, 285
MspR9I CCNGG 4 cut(s) 11, 68, 173, 299
Mva1269I GAATGC 2 cut(s) 159, 421
MvaI CCWGG 3 cut(s) 11, 173, 299
MwoI GCNNNNNNNGC 3 cut(s) 52, 101, 370
NaeI GCCGGC 2 cut(s) 93, 286
NarI GGCGCC 1 cut(s) 282
NciI CCSGG 1 cut(s) 68
NdeII GATC 2 cut(s) 357, 445
NgoMIV GCCGGC 2 cut(s) 91, 284
NlaIII CATG 2 cut(s) 130, 410
NlaIV GGNNCC 6 cut(s) 103, 104, 119, 184, 283, 399
NmeAIII GCCGAG 1 cut(s) 18
NmuCI GTSAC 1 cut(s) 19
NsiI ATGCAT 1 cut(s) 408
NspI RCATGY 1 cut(s) 410
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PctI GAATGC 2 cut(s) 159, 421
PdiI GCCGGC 2 cut(s) 93, 286
PdmI GAANNNNTTC 1 cut(s) 159
PflFI GACNNNGTC 1 cut(s) 17
PfoI TCCNGGA 2 cut(s) 171, 297
PkrI GCNGC 2 cut(s) 235, 375
PleI GAGTC 3 cut(s) 8, 117, 170
PluTI GGCGCC 1 cut(s) 285
PmaCI CACGTG 1 cut(s) 422
PmlI CACGTG 1 cut(s) 422
PpsI GAGTC 3 cut(s) 8, 117, 170
Ppu21I YACGTR 1 cut(s) 422
PshAI GACNNNNGTC 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 171
Psp6I CCWGG 3 cut(s) 9, 171, 297
PspCI CACGTG 1 cut(s) 422
PspFI CCCAGC 1 cut(s) 306
PspGI CCWGG 3 cut(s) 9, 171, 297
PspN4I GGNNCC 6 cut(s) 103, 104, 119, 184, 283, 399
PspOMI GGGCCC 1 cut(s) 102
PspPI GGNCC 8 cut(s) 4, 25, 102, 103, 118, 244, 249, 301
PsyI GACNNNGTC 1 cut(s) 17
RsaI GTAC 3 cut(s) 131, 316, 338
RsaNI GTAC 3 cut(s) 130, 315, 337
Rsr2I CGGWCCG 1 cut(s) 249
RsrII CGGWCCG 1 cut(s) 249
SacI GAGCTC 1 cut(s) 171
SatI GCNGC 2 cut(s) 234, 374
Sau3AI GATC 2 cut(s) 357, 445
Sau96I GGNCC 8 cut(s) 4, 25, 102, 103, 118, 244, 249, 301
SchI GAGTC 3 cut(s) 8, 117, 170
ScrFI CCNGG 4 cut(s) 11, 68, 173, 299
SduI GDGCHC 2 cut(s) 106, 171
SetI ASST 9 cut(s) 171, 231, 258, 297, 366, 385, 424, 459, 492
SfaNI GCATC 1 cut(s) 319
SfiI GGCCNNNNNGGCC 1 cut(s) 101
SfoI GGCGCC 1 cut(s) 283
SgrAI CRCCGGYG 1 cut(s) 284
SinI GGWCC 4 cut(s) 4, 118, 249, 301
Sse9I AATT 1 cut(s) 193
SsiI CCGC 7 cut(s) 7, 28, 219, 234, 304, 326, 332
SspDI GGCGCC 1 cut(s) 281
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 4 cut(s) 9, 66, 171, 297
StyI CCWWGG 2 cut(s) 450, 486
TaiI ACGT 3 cut(s) 231, 258, 424
TaqI TCGA 1 cut(s) 87
TaqII GACCGA 1 cut(s) 266
TasI AATT 1 cut(s) 193
TatI WGTACW 2 cut(s) 129, 336
TauI GCSGC 1 cut(s) 236
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 1 cut(s) 373
Tsp45I GTSAC 1 cut(s) 19
TspGWI ACGGA 2 cut(s) 7, 393
TspRI CASTG 1 cut(s) 205
Tth111I GACNNNGTC 1 cut(s) 17
VpaK11BI GGWCC 4 cut(s) 4, 118, 249, 301
XceI RCATGY 1 cut(s) 410
XmnI GAANNNNTTC 1 cut(s) 159
ZraI GACGTC 1 cut(s) 256
Zsp2I ATGCAT 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.