RchiOBHm_Chr3g0467641
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
N/A
Physical Location & Seq
Forward (+)
13744610 .. 13745514
905 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43359

Sequence Viewer

Length: 519 bp
ATGCCGGGAGAAGAAAAGGGCAAAGCAATAGGGATCGATCTGGGGACGACTTACTCGTGCGTGGCAGTGTGGAAGAACGATCATGTGGAAGTCATTGTGAACGATCAGGGCAACAGGACTACTCCTTCTTATGTTGCCTTCAATGATGCTGAACGATTGGTAGGGGATGCAGCATTTAACCAGGTCCTCAAAAACCCCACCAATTCCATCTTTGATGCTAAGCGGTTAATAGGTAGGAGATTCAGTGACCACATTGTTCAGCTTGATATGAAGTTGTGGCCATTCAAGGTCATTGAAGGTGGTGATGACAAGCCTACGATTGTGGTTACTCATGAGGGTCAGGAGAAACTGTTTGCTGCTGAAGAAATCTCCTCCATGGTTTTGGCAAAGATGCGGGACATTGCTGAGCAGTACCTTGACTCACCTGTGGAAGGTGCAGTTATCACCGTCCCTGCTTACTTCAGTGATTCGCAGCGCCAGGCTACAAGAAATGCGGGTGAAATTGCAGGCCTAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

172

Amino Acids

18.98

Weight (kDa)

5.09

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 9 - 171 3.1e-68 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 223, 394, 494
AclWI GGATC 1 cut(s) 41
AcoI YGGCCR 1 cut(s) 278
AcuI CTGAAG 2 cut(s) 381, 445
AfaI GTAC 1 cut(s) 413
AfiI CCNNNNNNNGG 1 cut(s) 431
AgsI TTSAA 3 cut(s) 142, 286, 296
AjnI CCWGG 2 cut(s) 180, 477
AluBI AGCT 1 cut(s) 262
AluI AGCT 1 cut(s) 262
AlwI GGATC 1 cut(s) 41
AoxI GGCC 2 cut(s) 278, 508
ApeKI GCWGC 3 cut(s) 170, 356, 472
AspLEI GCGC 1 cut(s) 477
AspS9I GGNCC 1 cut(s) 184
AsuC2I CCSGG 1 cut(s) 6
AsuHPI GGTGA 4 cut(s) 314, 414, 436, 509
AvaII GGWCC 1 cut(s) 184
AxyI CCTNAGG 1 cut(s) 512
BalI TGGCCA 1 cut(s) 280
BauI CACGAG 1 cut(s) 55
BbvI GCAGC 3 cut(s) 182, 343, 484
BccI CCATC 1 cut(s) 215
BciT130I CCWGG 2 cut(s) 182, 479
BcnI CCSGG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 478
BisI GCNGC 3 cut(s) 171, 357, 473
BlpI GCTNAGC 2 cut(s) 219, 405
BlsI GCNGC 3 cut(s) 172, 358, 474
Bme1390I CCNGG 3 cut(s) 6, 182, 479
Bme18I GGWCC 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 184
BmrFI CCNGG 3 cut(s) 6, 182, 479
BmsI GCATC 4 cut(s) 136, 157, 205, 381
Bpu1102I GCTNAGC 2 cut(s) 219, 405
BpuMI CCSGG 1 cut(s) 6
Bsa29I ATCGAT 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 375
BsaXI ACNNNNNCTCC 4 cut(s) 229, 259, 335, 365
Bsc4I CCNNNNNNNGG 1 cut(s) 431
Bse21I CCTNAGG 1 cut(s) 512
Bse3DI GCAATG 1 cut(s) 399
BseBI CCWGG 2 cut(s) 182, 479
BseCI ATCGAT 1 cut(s) 36
BseDI CCNNGG 1 cut(s) 375
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 1 cut(s) 431
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 1 cut(s) 396
BseRI GAGGAG 1 cut(s) 361
BseXI GCAGC 3 cut(s) 182, 343, 484
BsgI GTGCAG 1 cut(s) 456
BshFI GGCC 2 cut(s) 280, 510
BshVI ATCGAT 1 cut(s) 36
BsiSI CCGG 1 cut(s) 5
BslFI GGGAC 3 cut(s) 58, 410, 434
BslI CCNNNNNNNGG 1 cut(s) 431
BsmFI GGGAC 3 cut(s) 58, 410, 434
BsnI GGCC 2 cut(s) 280, 510
Bsp143I GATC 4 cut(s) 33, 37, 79, 103
Bsp1720I GCTNAGC 2 cut(s) 219, 405
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 3 cut(s) 223, 394, 494
BspANI GGCC 2 cut(s) 280, 510
BspCNI CTCAG 1 cut(s) 397
BspDI ATCGAT 1 cut(s) 36
BspHI TCATGA 1 cut(s) 331
BspPI GGATC 1 cut(s) 41
BsrDI GCAATG 1 cut(s) 399
BssECI CCNNGG 1 cut(s) 375
BssMI GATC 4 cut(s) 33, 37, 79, 103
BssSI CACGAG 1 cut(s) 55
BssT1I CCWWGG 1 cut(s) 375
Bst2BI CACGAG 1 cut(s) 55
Bst2UI CCWGG 2 cut(s) 182, 479
Bst4CI ACNGT 2 cut(s) 351, 448
BstC8I GCNNGC 1 cut(s) 508
BstDEI CTNAG 3 cut(s) 219, 405, 512
BstDSI CCRYGG 1 cut(s) 375
BstENI CCTNNNNNAGG 1 cut(s) 429
BstF5I GGATG 1 cut(s) 172
BstH2I RGCGCY 1 cut(s) 478
BstHHI GCGC 1 cut(s) 477
BstKTI GATC 4 cut(s) 36, 40, 82, 106
BstMBI GATC 4 cut(s) 33, 37, 79, 103
BstNI CCWGG 2 cut(s) 182, 479
BstSCI CCNGG 3 cut(s) 4, 180, 477
BstV1I GCAGC 3 cut(s) 182, 343, 484
BstXI CCANNNNNNTGG 1 cut(s) 382
Bsu15I ATCGAT 1 cut(s) 36
Bsu36I CCTNAGG 1 cut(s) 512
BsuRI GGCC 2 cut(s) 280, 510
BsuTUI ATCGAT 1 cut(s) 36
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 1 cut(s) 172
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 3 cut(s) 72, 250, 469
Cac8I GCNNGC 1 cut(s) 508
CciI TCATGA 1 cut(s) 331
CfoI GCGC 1 cut(s) 477
Cfr13I GGNCC 1 cut(s) 184
ClaI ATCGAT 1 cut(s) 36
CsiI ACCWGGT 1 cut(s) 180
Csp6I GTAC 1 cut(s) 412
CviAII CATG 3 cut(s) 83, 332, 376
CviJI RGCY 5 cut(s) 262, 280, 313, 482, 510
CviKI_1 RGCY 5 cut(s) 262, 280, 313, 482, 510
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 3 cut(s) 219, 405, 512
DpnI GATC 4 cut(s) 35, 39, 81, 105
DpnII GATC 4 cut(s) 33, 37, 79, 103
EaeI YGGCCR 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 375
Eco147I AGGCCT 1 cut(s) 510
Eco47I GGWCC 1 cut(s) 184
Eco57I CTGAAG 2 cut(s) 381, 445
Eco81I CCTNAGG 1 cut(s) 512
EcoNI CCTNNNNNAGG 1 cut(s) 429
EcoO109I RGGNCCY 1 cut(s) 184
EcoRII CCWGG 2 cut(s) 180, 477
EcoT14I CCWWGG 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 3 cut(s) 86, 335, 379
FaiI YATR 5 cut(s) 84, 132, 269, 333, 377
FaqI GGGAC 3 cut(s) 58, 410, 434
FatI CATG 3 cut(s) 82, 331, 375
FauI CCCGC 2 cut(s) 387, 487
Fnu4HI GCNGC 3 cut(s) 171, 357, 473
FokI GGATG 1 cut(s) 179
Fsp4HI GCNGC 3 cut(s) 171, 357, 473
GlaI GCGC 1 cut(s) 476
GluI GCNGC 3 cut(s) 171, 357, 473
HaeII RGCGCY 1 cut(s) 478
HaeIII GGCC 2 cut(s) 280, 510
HapII CCGG 1 cut(s) 5
HhaI GCGC 1 cut(s) 477
Hin1II CATG 3 cut(s) 86, 335, 379
Hin6I GCGC 1 cut(s) 475
HinP1I GCGC 1 cut(s) 475
HinfI GANTC 3 cut(s) 240, 419, 467
HpaII CCGG 1 cut(s) 5
HphI GGTGA 4 cut(s) 314, 414, 436, 509
Hpy166II GTNNAC 1 cut(s) 100
Hpy188III TCNNGA 2 cut(s) 332, 341
Hpy8I GTNNAC 1 cut(s) 100
HpyAV CCTTC 4 cut(s) 135, 148, 290, 425
HpyCH4III ACNGT 2 cut(s) 351, 448
HpyCH4V TGCA 3 cut(s) 170, 437, 506
HpyF3I CTNAG 3 cut(s) 219, 405, 512
Hsp92II CATG 3 cut(s) 86, 335, 379
HspAI GCGC 1 cut(s) 475
Kzo9I GATC 4 cut(s) 33, 37, 79, 103
Lsp1109I GCAGC 3 cut(s) 182, 343, 484
LweI GCATC 4 cut(s) 136, 157, 205, 381
MabI ACCWGGT 1 cut(s) 180
MaeIII GTNAC 2 cut(s) 245, 325
MalI GATC 4 cut(s) 35, 39, 81, 105
MboI GATC 4 cut(s) 33, 37, 79, 103
MboII GAAGA 3 cut(s) 23, 85, 374
MlsI TGGCCA 1 cut(s) 280
MluCI AATT 2 cut(s) 202, 501
MluNI TGGCCA 1 cut(s) 280
MlyI GAGTC 1 cut(s) 413
MnlI CCTC 3 cut(s) 197, 328, 382
Mox20I TGGCCA 1 cut(s) 280
MscI TGGCCA 1 cut(s) 280
MseI TTAA 2 cut(s) 177, 227
Msp20I TGGCCA 1 cut(s) 280
MspI CCGG 1 cut(s) 5
MspR9I CCNGG 3 cut(s) 6, 182, 479
MvaI CCWGG 2 cut(s) 182, 479
NciI CCSGG 1 cut(s) 6
NcoI CCATGG 1 cut(s) 375
NdeII GATC 4 cut(s) 33, 37, 79, 103
NlaIII CATG 3 cut(s) 86, 335, 379
NmuCI GTSAC 1 cut(s) 245
PagI TCATGA 1 cut(s) 331
PceI AGGCCT 1 cut(s) 510
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PfeI GAWTC 2 cut(s) 240, 467
PkrI GCNGC 3 cut(s) 172, 358, 474
PleI GAGTC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 413
PpuMI RGGWCCY 1 cut(s) 184
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 180, 477
PspGI CCWGG 2 cut(s) 180, 477
PspPI GGNCC 1 cut(s) 184
PspPPI RGGWCCY 1 cut(s) 184
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
SaqAI TTAA 2 cut(s) 177, 227
SatI GCNGC 3 cut(s) 171, 357, 473
Sau3AI GATC 4 cut(s) 33, 37, 79, 103
Sau96I GGNCC 1 cut(s) 184
SchI GAGTC 1 cut(s) 413
ScrFI CCNGG 3 cut(s) 6, 182, 479
SetI ASST 9 cut(s) 186, 235, 264, 291, 301, 417, 427, 436, 518
SexAI ACCWGGT 1 cut(s) 180
SfaNI GCATC 4 cut(s) 136, 157, 205, 381
SinI GGWCC 1 cut(s) 184
Sse9I AATT 2 cut(s) 202, 501
SseBI AGGCCT 1 cut(s) 510
SsiI CCGC 3 cut(s) 223, 394, 494
StuI AGGCCT 1 cut(s) 510
StyD4I CCNGG 3 cut(s) 4, 180, 477
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 2 cut(s) 351, 448
TaqI TCGA 1 cut(s) 36
TasI AATT 2 cut(s) 202, 501
TfiI GAWTC 2 cut(s) 240, 467
Tru1I TTAA 2 cut(s) 177, 227
Tru9I TTAA 2 cut(s) 177, 227
TscAI CASTG 3 cut(s) 72, 250, 469
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 3 cut(s) 170, 356, 472
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 1 cut(s) 284
TspRI CASTG 3 cut(s) 72, 250, 469
VpaK11BI GGWCC 1 cut(s) 184
XagI CCTNNNNNAGG 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.