RchiOBHm_Chr4g0414171

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
N/A
Physical Location & Seq
Reverse (-)
38558982 .. 38562899
3918 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38461

Sequence Viewer

Length: 1389 bp
ATGGCATCCCTCCGCGAATTGGAGATAGAAGATTGTGATGGATTATCTAGTATTGGCCTACTCGATCACTGCACCTCTCTACAGGAATTGGAGATACGCAGTTGTGGAAATCTAACATCGATTCCAATCTCACAAGGCCTTGCATCTCTCCGCAAATTGATTATTTGGAAGTGTGGTGGATTATCTAGTATTGGCATACTCGATCACTGCACCTCTCTACAAGAATTGGAGATAAGCGGATGTGGAAATCTAACATCGATTCCAATCTCACAAGGCCTTGCATCTCTCCGCAAATTGGAGATAGAAGATTGTGATGGATTATCTAGTATTGGCATACTCGATCACTGCACCTCTCTGCAGGAATTGCAGATAAGCATATGTGGAAATCTAACATCCATTCCAATCTCACAAGGCCTTGCATCTCTCCGCACATTGGGTATTTACAGGTGTGGTGGGTTAACGGGCCTACCGTGCGGGATTGAAAACCCCTCTCTTCAGGATTTAATGATATCCGGTTTCTCAAGTCTAGAGACCATTTCGTTTACACGTGTCCTCCCATCTATCCGCATGTTGCAAGTTACGGGATGCGATGAATTATCTTGCTTGGCTGTCCTCGAATACTGTCCCTCGCTTCAGAAATTGAGTGTAAGCAAATGCCCCAACCTAGCACCCATTTCATTTTCTGCGGATTTGAATCCATGTCTTAAGGAGTTGCGTATAGATGATTGCAATGGTCTACAATCCCTTCCAGATTTCCGCAGTTTCACATCCCTCCTTGTATTGGAGATTGAAGAATGTACGAGTCTGGTATGTGGGTTTCATATCCCGGTGTCTCTGGAGGAGTTGCGGATTTCTGAATGCCATAACCTAGAGAGTATTCCAAGTTTAGATGAATGCACATCCCTCCGTCGGTTACAGGTTCGTGATTGTGAGAAACTGAAGTCTCTGTCAACAGGGCTGCGCTGCCTCGCTCGTCTGGAGAATTTGGATTTAGGTCCTTTTTGGGAGGAGCTCGACTCCTTCCCTGATTTTCAAGCTCCATCACAACAACTTGACGAATTAGAGTTGAAAGGGTGGCCTAAACTCAAGTCTCTTCCTCAACAAGTTCAACACTTCACTTCGCTGACAAAGTTGACTATAAGATCTTTCAATGGAGTGGAGGCTTTACCAGAGTGGTTGGGCAACCTTGCATCTCTCAAGCACCTGACTATTTCGAATTGTGAGAATCTCAAGTATCTACCTTCACTCAAAGCAATGCAACGCCTCACCAAATTATATAGTCTAGAGATTTGTAGGTGTCCACTGCTAAAGGAAAGATGCACCAAGGACAGCGGCGAAGAATGGCCCAAGATTTCTCACATTCGAAATATCCATATTTGGCGGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 ko04014 map03460 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

462

Amino Acids

51.13

Weight (kDa)

5.13

Isoelectric Point (pI)

60.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 3 - 105 8.7e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_5 PF13306 11 - 130 4.5e-09 BspA type Leucine rich repeat region (6 copies)
LRR_5 PF13306 57 - 154 1.9e-06 BspA type Leucine rich repeat region (6 copies)
LRR_14 PF23598 235 - 380 9.1e-07 Leucine-rich repeat region
LRR_14 PF23598 289 - 461 1.2e-10 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 370 - 426 2.3e-09 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 736
AccII CGCG 1 cut(s) 15
AcsI RAATTY 1 cut(s) 982
AcuI CTGAAG 3 cut(s) 479, 617, 959
AcvI CACGTG 1 cut(s) 548
AfaI GTAC 1 cut(s) 799
AfiI CCNNNNNNNGG 5 cut(s) 19, 295, 433, 781, 909
AflII CTTAAG 1 cut(s) 704
AflIII ACRYGT 2 cut(s) 545, 547
AgsI TTSAA 7 cut(s) 482, 694, 791, 1034, 1069, 1109, 1150
AluBI AGCT 2 cut(s) 1012, 1037
AluI AGCT 2 cut(s) 1012, 1037
Alw21I GWGCWC 1 cut(s) 1014
Alw26I GTCTC 4 cut(s) 524, 837, 948, 1095
AoxI GGCC 7 cut(s) 55, 136, 274, 412, 463, 1076, 1343
ApeKI GCWGC 2 cut(s) 958, 963
ApoI RAATTY 1 cut(s) 982
AspLEI GCGC 1 cut(s) 963
AspS9I GGNCC 3 cut(s) 463, 995, 1344
AsuC2I CCSGG 1 cut(s) 827
AsuHPI GGTGA 1 cut(s) 1258
AsuII TTCGAA 2 cut(s) 1214, 1363
AvaII GGWCC 1 cut(s) 995
BanII GRGCYC 1 cut(s) 1014
BbrPI CACGTG 1 cut(s) 548
Bbv12I GWGCWC 1 cut(s) 1014
BbvI GCAGC 2 cut(s) 945, 950
BccI CCATC 4 cut(s) 32, 308, 565, 1048
BcnI CCSGG 1 cut(s) 827
BcoDI GTCTC 4 cut(s) 524, 837, 948, 1095
BfaI CTAG 7 cut(s) 48, 186, 324, 527, 665, 869, 1283
BfmI CTRYAG 2 cut(s) 80, 356
BfrI CTTAAG 1 cut(s) 704
BglII AGATCT 1 cut(s) 1142
BisI GCNGC 3 cut(s) 959, 964, 1333
BlsI GCNGC 3 cut(s) 960, 965, 1334
Bme1390I CCNGG 1 cut(s) 827
Bme18I GGWCC 1 cut(s) 995
BmgT120I GGNCC 3 cut(s) 463, 995, 1344
BmrFI CCNGG 1 cut(s) 827
BmsI GCATC 7 cut(s) 14, 152, 290, 428, 575, 1199, 1307
BplI GAGNNNNNCTC 2 cut(s) 1001, 1033
BpmI CTGGAG 2 cut(s) 857, 998
Bpu14I TTCGAA 2 cut(s) 1214, 1363
BpuEI CTTGAG 4 cut(s) 505, 1070, 1181, 1214
BpuMI CCSGG 1 cut(s) 827
Bsa29I ATCGAT 2 cut(s) 119, 257
BsaAI YACGTR 1 cut(s) 548
BsaBI GATNNNNATC 3 cut(s) 125, 263, 693
BsaI GGTCTC 1 cut(s) 524
BsaJI CCNNGG 1 cut(s) 1323
BsaWI WCCGGW 1 cut(s) 512
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 295, 433, 781, 909
Bse3DI GCAATG 2 cut(s) 736, 1260
Bse8I GATNNNNATC 3 cut(s) 125, 263, 693
BseCI ATCGAT 2 cut(s) 119, 257
BseDI CCNNGG 1 cut(s) 1323
BseGI GGATG 6 cut(s) 5, 245, 392, 590, 767, 899
BseJI GATNNNNATC 3 cut(s) 125, 263, 693
BseLI CCNNNNNNNGG 5 cut(s) 19, 295, 433, 781, 909
BseMI GCAATG 2 cut(s) 736, 1260
BseRI GAGGAG 2 cut(s) 854, 1022
BseXI GCAGC 2 cut(s) 945, 950
BsgI GTGCAG 3 cut(s) 55, 193, 331
Bsh1236I CGCG 1 cut(s) 15
BshFI GGCC 7 cut(s) 57, 138, 276, 414, 465, 1078, 1345
BshVI ATCGAT 2 cut(s) 119, 257
BsiHKAI GWGCWC 1 cut(s) 1014
BsiSI CCGG 2 cut(s) 513, 827
BslFI GGGAC 1 cut(s) 609
BslI CCNNNNNNNGG 5 cut(s) 19, 295, 433, 781, 909
BsmAI GTCTC 4 cut(s) 524, 837, 948, 1095
BsmFI GGGAC 1 cut(s) 609
BsmI GAATGC 2 cut(s) 863, 899
BsnI GGCC 7 cut(s) 57, 138, 276, 414, 465, 1078, 1345
Bso31I GGTCTC 1 cut(s) 524
Bsp119I TTCGAA 2 cut(s) 1214, 1363
Bsp1286I GDGCHC 1 cut(s) 1014
Bsp143I GATC 4 cut(s) 64, 202, 340, 1142
BspANI GGCC 7 cut(s) 57, 138, 276, 414, 465, 1078, 1345
BspDI ATCGAT 2 cut(s) 119, 257
BspFNI CGCG 1 cut(s) 15
BspMAI CTGCAG 1 cut(s) 360
BspT104I TTCGAA 2 cut(s) 1214, 1363
BspTI CTTAAG 1 cut(s) 704
BspTNI GGTCTC 1 cut(s) 524
BsrDI GCAATG 2 cut(s) 736, 1260
BssECI CCNNGG 1 cut(s) 1323
BssMI GATC 4 cut(s) 64, 202, 340, 1142
BssT1I CCWWGG 1 cut(s) 1323
Bst4CI ACNGT 2 cut(s) 471, 623
Bst6I CTCTTC 2 cut(s) 498, 1098
BstAFI CTTAAG 1 cut(s) 704
BstAPI GCANNNNNTGC 1 cut(s) 364
BstBAI YACGTR 1 cut(s) 548
BstBI TTCGAA 2 cut(s) 1214, 1363
BstF5I GGATG 6 cut(s) 5, 245, 392, 590, 767, 899
BstFNI CGCG 1 cut(s) 15
BstHHI GCGC 1 cut(s) 963
BstKTI GATC 4 cut(s) 67, 205, 343, 1145
BstMAI GTCTC 4 cut(s) 524, 837, 948, 1095
BstMBI GATC 4 cut(s) 64, 202, 340, 1142
BstMWI GCNNNNNNNGC 2 cut(s) 364, 471
BstNSI RCATGY 1 cut(s) 571
BstSCI CCNGG 1 cut(s) 825
BstSFI CTRYAG 2 cut(s) 80, 356
BstUI CGCG 1 cut(s) 15
BstV1I GCAGC 2 cut(s) 945, 950
BstX2I RGATCY 1 cut(s) 1142
BstYI RGATCY 1 cut(s) 1142
Bsu15I ATCGAT 2 cut(s) 119, 257
BsuRI GGCC 7 cut(s) 57, 138, 276, 414, 465, 1078, 1345
BsuTUI ATCGAT 2 cut(s) 119, 257
BtgZI GCGATG 1 cut(s) 603
BtsCI GGATG 6 cut(s) 5, 245, 392, 590, 767, 899
BtsI GCAGTG 4 cut(s) 67, 205, 343, 1301
BtsIMutI CAGTG 4 cut(s) 67, 205, 343, 1301
CfoI GCGC 1 cut(s) 963
Cfr13I GGNCC 3 cut(s) 463, 995, 1344
ClaI ATCGAT 2 cut(s) 119, 257
Csp6I GTAC 1 cut(s) 798
CviAII CATG 2 cut(s) 568, 699
CviQI GTAC 1 cut(s) 798
DpnI GATC 4 cut(s) 66, 204, 342, 1144
DpnII GATC 4 cut(s) 64, 202, 340, 1142
Eam1104I CTCTTC 2 cut(s) 498, 1098
EarI CTCTTC 2 cut(s) 498, 1098
Ecl136II GAGCTC 1 cut(s) 1012
Eco130I CCWWGG 1 cut(s) 1323
Eco147I AGGCCT 3 cut(s) 138, 276, 414
Eco24I GRGCYC 1 cut(s) 1014
Eco31I GGTCTC 1 cut(s) 524
Eco32I GATATC 1 cut(s) 510
Eco47I GGWCC 1 cut(s) 995
Eco53kI GAGCTC 1 cut(s) 1012
Eco57I CTGAAG 3 cut(s) 479, 617, 959
Eco72I CACGTG 1 cut(s) 548
EcoICRI GAGCTC 1 cut(s) 1012
EcoO109I RGGNCCY 1 cut(s) 995
EcoRV GATATC 1 cut(s) 510
EcoT14I CCWWGG 1 cut(s) 1323
EcoT38I GRGCYC 1 cut(s) 1014
ErhI CCWWGG 1 cut(s) 1323
FaeI CATG 2 cut(s) 571, 702
FaqI GGGAC 1 cut(s) 609
FatI CATG 2 cut(s) 567, 698
FauI CCCGC 2 cut(s) 467, 1374
FauNDI CATATG 1 cut(s) 377
FblI GTMKAC 1 cut(s) 736
Fnu4HI GCNGC 3 cut(s) 959, 964, 1333
FokI GGATG 5 cut(s) 252, 379, 597, 754, 886
FriOI GRGCYC 1 cut(s) 1014
Fsp4HI GCNGC 3 cut(s) 959, 964, 1333
FspBI CTAG 7 cut(s) 48, 186, 324, 527, 665, 869, 1283
GlaI GCGC 1 cut(s) 962
GluI GCNGC 3 cut(s) 959, 964, 1333
GsuI CTGGAG 2 cut(s) 857, 998
HaeIII GGCC 7 cut(s) 57, 138, 276, 414, 465, 1078, 1345
HapII CCGG 2 cut(s) 513, 827
HhaI GCGC 1 cut(s) 963
Hin1II CATG 2 cut(s) 571, 702
Hin6I GCGC 1 cut(s) 961
HinP1I GCGC 1 cut(s) 961
HincII GTYRAC 3 cut(s) 459, 951, 1134
HindII GTYRAC 3 cut(s) 459, 951, 1134
HinfI GANTC 6 cut(s) 121, 259, 694, 802, 1016, 1225
HpaI GTTAAC 1 cut(s) 459
HpaII CCGG 2 cut(s) 513, 827
HphI GGTGA 1 cut(s) 1258
Hpy166II GTNNAC 6 cut(s) 459, 543, 737, 951, 1134, 1301
Hpy188I TCNGA 2 cut(s) 636, 856
Hpy188III TCNNGA 7 cut(s) 497, 527, 749, 836, 923, 977, 1283
Hpy8I GTNNAC 6 cut(s) 459, 543, 737, 951, 1134, 1301
Hpy99I CGWCG 1 cut(s) 912
HpyAV CCTTC 3 cut(s) 755, 1030, 1251
HpyCH4III ACNGT 2 cut(s) 471, 623
HpyCH4IV ACGT 1 cut(s) 547
HpyF10VI GCNNNNNNNGC 2 cut(s) 364, 471
HpySE526I ACGT 1 cut(s) 547
Hsp92II CATG 2 cut(s) 571, 702
HspAI GCGC 1 cut(s) 961
KspAI GTTAAC 1 cut(s) 459
Kzo9I GATC 4 cut(s) 64, 202, 340, 1142
LmnI GCTCC 2 cut(s) 1009, 1042
Lsp1109I GCAGC 2 cut(s) 945, 950
LweI GCATC 7 cut(s) 14, 152, 290, 428, 575, 1199, 1307
MaeI CTAG 7 cut(s) 48, 186, 324, 527, 665, 869, 1283
MaeII ACGT 1 cut(s) 547
MaeIII GTNAC 2 cut(s) 577, 912
MalI GATC 4 cut(s) 66, 204, 342, 1144
MboI GATC 4 cut(s) 64, 202, 340, 1142
MboII GAAGA 6 cut(s) 41, 317, 485, 803, 1085, 1349
MflI RGATCY 1 cut(s) 1142
MhlI GDGCHC 1 cut(s) 1014
MlyI GAGTC 2 cut(s) 811, 1010
MseI TTAA 3 cut(s) 458, 503, 705
MspA1I CMGCKG 1 cut(s) 1332
MspCI CTTAAG 1 cut(s) 704
MspI CCGG 2 cut(s) 513, 827
MspR9I CCNGG 1 cut(s) 827
Mva1269I GAATGC 2 cut(s) 863, 899
MvnI CGCG 1 cut(s) 15
MwoI GCNNNNNNNGC 2 cut(s) 364, 471
NciI CCSGG 1 cut(s) 827
NdeI CATATG 1 cut(s) 377
NdeII GATC 4 cut(s) 64, 202, 340, 1142
NlaIII CATG 2 cut(s) 571, 702
NspI RCATGY 1 cut(s) 571
NspV TTCGAA 2 cut(s) 1214, 1363
PceI AGGCCT 3 cut(s) 138, 276, 414
PcsI WCGNNNNNNNCGW 1 cut(s) 467
PctI GAATGC 2 cut(s) 863, 899
PfeI GAWTC 4 cut(s) 121, 259, 694, 1225
PkrI GCNGC 3 cut(s) 960, 965, 1334
PleI GAGTC 2 cut(s) 810, 1010
PmaCI CACGTG 1 cut(s) 548
PmlI CACGTG 1 cut(s) 548
PpsI GAGTC 2 cut(s) 810, 1010
Ppu21I YACGTR 1 cut(s) 548
PpuMI RGGWCCY 1 cut(s) 995
Psp124BI GAGCTC 1 cut(s) 1014
Psp5II RGGWCCY 1 cut(s) 995
PspCI CACGTG 1 cut(s) 548
PspPI GGNCC 3 cut(s) 463, 995, 1344
PspPPI RGGWCCY 1 cut(s) 995
PstI CTGCAG 1 cut(s) 360
PsuI RGATCY 1 cut(s) 1142
RsaI GTAC 1 cut(s) 799
RsaNI GTAC 1 cut(s) 798
SacI GAGCTC 1 cut(s) 1014
SaqAI TTAA 3 cut(s) 458, 503, 705
SatI GCNGC 3 cut(s) 959, 964, 1333
Sau3AI GATC 4 cut(s) 64, 202, 340, 1142
Sau96I GGNCC 3 cut(s) 463, 995, 1344
SchI GAGTC 2 cut(s) 811, 1010
ScrFI CCNGG 1 cut(s) 827
SduI GDGCHC 1 cut(s) 1014
SfaNI GCATC 7 cut(s) 14, 152, 290, 428, 575, 1199, 1307
SfcI CTRYAG 2 cut(s) 80, 356
SfuI TTCGAA 2 cut(s) 1214, 1363
SinI GGWCC 1 cut(s) 995
SmlI CTYRAG 5 cut(s) 520, 704, 1085, 1196, 1229
SmoI CTYRAG 5 cut(s) 520, 704, 1085, 1196, 1229
SseBI AGGCCT 3 cut(s) 138, 276, 414
SspMI CTAG 7 cut(s) 48, 186, 324, 527, 665, 869, 1283
SstI GAGCTC 1 cut(s) 1014
StuI AGGCCT 3 cut(s) 138, 276, 414
StyD4I CCNGG 1 cut(s) 825
StyI CCWWGG 1 cut(s) 1323
TaaI ACNGT 2 cut(s) 471, 623
TaiI ACGT 1 cut(s) 550
TaqI TCGA 9 cut(s) 63, 119, 201, 257, 339, 615, 1014, 1214, 1363
TauI GCSGC 1 cut(s) 1335
TfiI GAWTC 4 cut(s) 121, 259, 694, 1225
Tru1I TTAA 3 cut(s) 458, 503, 705
Tru9I TTAA 3 cut(s) 458, 503, 705
TscAI CASTG 4 cut(s) 74, 212, 350, 1308
TseI GCWGC 2 cut(s) 958, 963
TspDTI ATGAA 4 cut(s) 606, 666, 809, 906
TspGWI ACGGA 1 cut(s) 896
TspRI CASTG 4 cut(s) 74, 212, 350, 1308
Vha464I CTTAAG 1 cut(s) 704
VpaK11BI GGWCC 1 cut(s) 995
XapI RAATTY 1 cut(s) 982
XbaI TCTAGA 2 cut(s) 526, 1282
XceI RCATGY 1 cut(s) 571
XmiI GTMKAC 1 cut(s) 736
XspI CTAG 7 cut(s) 48, 186, 324, 527, 665, 869, 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.