RchiOBHm_Chr4g0417111

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
N/A
Physical Location & Seq
Forward (+)
42321730 .. 42323212
1483 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38720

Sequence Viewer

Length: 1395 bp
ATGGGTGCTAGATTTCCGTCATGGATATCAGCTGAAGGAGGAGAGGTCGTGAAGGTATTTCCTTGCCTCGAAGAGTTGAGCATAGACAATTGTCACAGCCTAGAGTCGATTCGGATCACACAGGGCATCGCATCTCTCCTTGAATTGAAGATTTGCGATTGTTGGAGATTATCAAGCCTAGAGGTTGGGCTCCATAACTGCACCTCTCTTCAGGAGTTGGAGATACGTGTTTGCCCTGTTCTGGTTTCAATTCCAATCCCACAGGGCATGCCGTCTCTCCAGAAATTGAATCTCTTCAAGTGTAATGGATTGTCAAGCCTAGGAAGTGGGCTCAGCTACTGCACCTCTCTTCAGGAGTTGGAGATAAAGAATTGTGACAATCTAACATCAATTCCAATCACACAGGGCATGCCGTCTCTCCGGAAATTGAATCTCTTCAAGTGTAATGGATTGTCAAGCCTAGGAAGTGGGCTCAACTACTGCACCTCTCTTCAGGAATTGGAGATAAAGGATTGTTACAAACTAAGATCAATTCCAATCACACAGGGCATCGCATCTCTCCGTCAATTGAACATCTGGGCCTGTAGGGGATTATCGAGCCTAGGAGGTGGGCTCAACTACTGCACCTCTCTTCAGGAATTGGAGATAAGGGATTGTGATGAATTGACATCAATTCCAATCACACAGGGCATCACATCTCTCCGAGAATTAAAGATTGATAGTTGTTGGAGTTTATTAAGCCTATCGAGTGGGCTCCAATTCTGCACCTCTCTTGAGGATCTGGTTATAACTGATTGCTCTCATCTAGTATCCTTGTCAGTATCATATGTGAAGTGCGACCACCCGGAGGAAATTTCTCAAGGTGTTGATGCGTATTTGTCTGTACCGGAGGAGGATTGCCGTTCTGTAGAATCTATTCTTGCTCGGTTGTTGGAATTAAATTATGTGCCCACAGTAGAATTTCACCACCTCACTCGTTTGAAGACACTCCGAATTGGTGCGTTCTGGGAGGAGCTGGACGCCTTCCCTCATTTTCATCTTCCTCCTAATTCACAACTTGTTGAGTTATGGTTGTTTGGGTGGCCTACGCTCGAGTCTCTGCCCCAACAAATTCAGCACTTGACTTGTTTAAAACAACTCTGGATATGTGATTTTAAGGGACTAGAGGCTCTTCCTGAGTGGTTGGGAAATCTTACATCTCTTGAGTCTCTGAGTATCTGCTTCTGTGAGAATCTCAAGTATCTGCCTACACAAAATGCTATGAAAAGCCTCACCAAATTAAACAAGCTCCGCAGTGACGGATGTCCCCTGCTCAAAGAAAGATGCACCAAGGAGACTGGCCCAGACTGGCCCAAGATTTCTCACATCCCAGATCTTCAGTTTGGCTATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 ko04014 map03460 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

464

Amino Acids

51.92

Weight (kDa)

5.2

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 364 - 431 4.3e-07 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 370 - 428 1.6e-06 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 788
AccIII TCCGGA 1 cut(s) 420
AciI CCGC 1 cut(s) 1291
AclWI GGATC 2 cut(s) 122, 786
AcsI RAATTY 3 cut(s) 852, 959, 1110
AcuI CTGAAG 6 cut(s) 54, 194, 335, 476, 617, 1361
AcyI GRCGYC 1 cut(s) 1020
AfaI GTAC 1 cut(s) 885
AfiI CCNNNNNNNGG 2 cut(s) 241, 847
AflIII ACRYGT 1 cut(s) 226
AgsI TTSAA 9 cut(s) 143, 148, 249, 289, 298, 430, 439, 571, 982
AluBI AGCT 4 cut(s) 32, 336, 1015, 1288
AluI AGCT 4 cut(s) 32, 336, 1015, 1288
Alw26I GTCTC 5 cut(s) 279, 420, 1101, 1212, 1328
AlwI GGATC 2 cut(s) 122, 786
AlwNI CAGNNNCTG 1 cut(s) 339
Ama87I CYCGRG 1 cut(s) 1091
Aor13HI TCCGGA 1 cut(s) 420
AoxI GGCC 4 cut(s) 579, 1082, 1339, 1349
ApoI RAATTY 3 cut(s) 852, 959, 1110
Asp700I GAANNNNTTC 3 cut(s) 293, 434, 915
AspA2I CCTAGG 3 cut(s) 319, 460, 601
AspS9I GGNCC 3 cut(s) 579, 1340, 1350
AsuC2I CCSGG 1 cut(s) 845
AsuHPI GGTGA 2 cut(s) 956, 1264
AvaI CYCGRG 1 cut(s) 1091
AvrII CCTAGG 3 cut(s) 319, 460, 601
BaeGI GKGCMC 1 cut(s) 951
BanII GRGCYC 5 cut(s) 192, 333, 474, 615, 756
BbsI GAAGAC 1 cut(s) 989
BceAI ACGGC 3 cut(s) 256, 397, 885
BciVI GTATCC 1 cut(s) 820
BcnI CCSGG 1 cut(s) 845
BcoDI GTCTC 5 cut(s) 279, 420, 1101, 1212, 1328
BfaI CTAG 8 cut(s) 9, 101, 179, 320, 461, 602, 806, 1163
BfmI CTRYAG 2 cut(s) 583, 906
BfuI GTATCC 1 cut(s) 820
BglII AGATCT 1 cut(s) 1372
BlnI CCTAGG 3 cut(s) 319, 460, 601
BlpI GCTNAGC 1 cut(s) 332
Bme1390I CCNGG 1 cut(s) 845
BmeT110I CYCGRG 1 cut(s) 1091
BmgT120I GGNCC 3 cut(s) 579, 1340, 1350
BmiI GGNNCC 2 cut(s) 191, 755
BmrFI CCNGG 1 cut(s) 845
BmsI GCATC 7 cut(s) 135, 140, 558, 563, 699, 859, 1313
BoxI GACNNNNGTC 2 cut(s) 90, 1302
BpiI GAAGAC 1 cut(s) 989
BplI GAGNNNNNCTC 2 cut(s) 597, 629
BpmI CTGGAG 1 cut(s) 263
Bpu1102I GCTNAGC 1 cut(s) 332
BpuEI CTTGAG 4 cut(s) 794, 843, 1220, 1223
BpuMI CCSGG 1 cut(s) 845
BsaAI YACGTR 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 113
BsaHI GRCGYC 1 cut(s) 1020
BsaJI CCNNGG 4 cut(s) 319, 460, 601, 1329
BsaWI WCCGGW 2 cut(s) 420, 886
BsaXI ACNNNNNCTCC 4 cut(s) 30, 33, 60, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 847
Bse1I ACTGG 2 cut(s) 1342, 1352
Bse8I GATNNNNATC 1 cut(s) 113
BseAI TCCGGA 1 cut(s) 420
BseDI CCNNGG 4 cut(s) 319, 460, 601, 1329
BseGI GGATG 2 cut(s) 1307, 1365
BseJI GATNNNNATC 1 cut(s) 113
BseLI CCNNNNNNNGG 2 cut(s) 241, 847
BseMII CTCAG 3 cut(s) 346, 1167, 1202
BseNI ACTGG 2 cut(s) 1342, 1352
BseRI GAGGAG 3 cut(s) 54, 905, 1025
BseSI GKGCMC 1 cut(s) 951
BsgI GTGCAG 5 cut(s) 184, 325, 466, 607, 748
BshFI GGCC 4 cut(s) 581, 1084, 1341, 1351
BsiHKCI CYCGRG 1 cut(s) 1091
BsiSI CCGG 3 cut(s) 421, 845, 887
BslFI GGGAC 2 cut(s) 1173, 1290
BslI CCNNNNNNNGG 2 cut(s) 241, 847
BsmAI GTCTC 5 cut(s) 279, 420, 1101, 1212, 1328
BsmBI CGTCTC 2 cut(s) 279, 420
BsmFI GGGAC 2 cut(s) 1173, 1290
BsnI GGCC 4 cut(s) 581, 1084, 1341, 1351
BsoBI CYCGRG 1 cut(s) 1091
Bsp1286I GDGCHC 6 cut(s) 192, 333, 474, 615, 756, 951
Bsp13I TCCGGA 1 cut(s) 420
Bsp143I GATC 4 cut(s) 114, 527, 778, 1372
Bsp1720I GCTNAGC 1 cut(s) 332
BspACI CCGC 1 cut(s) 1291
BspANI GGCC 4 cut(s) 581, 1084, 1341, 1351
BspCNI CTCAG 3 cut(s) 345, 1168, 1203
BspEI TCCGGA 1 cut(s) 420
BspLI GGNNCC 2 cut(s) 191, 755
BspPI GGATC 2 cut(s) 122, 786
BspQI GCTCTTC 1 cut(s) 1176
BsrI ACTGG 2 cut(s) 1342, 1352
BssECI CCNNGG 4 cut(s) 319, 460, 601, 1329
BssMI GATC 4 cut(s) 114, 527, 778, 1372
BssNI GRCGYC 1 cut(s) 1020
BssT1I CCWWGG 4 cut(s) 319, 460, 601, 1329
Bst4CI ACNGT 1 cut(s) 955
Bst6I CTCTTC 8 cut(s) 66, 213, 299, 354, 440, 495, 636, 1176
BstACI GRCGYC 1 cut(s) 1020
BstBAI YACGTR 1 cut(s) 227
BstC8I GCNNGC 2 cut(s) 269, 410
BstDEI CTNAG 4 cut(s) 332, 524, 1176, 1211
BstF5I GGATG 2 cut(s) 1307, 1365
BstKTI GATC 4 cut(s) 117, 530, 781, 1375
BstMAI GTCTC 5 cut(s) 279, 420, 1101, 1212, 1328
BstMBI GATC 4 cut(s) 114, 527, 778, 1372
BstNSI RCATGY 2 cut(s) 271, 412
BstPAI GACNNNNGTC 2 cut(s) 90, 1302
BstSCI CCNGG 1 cut(s) 843
BstSFI CTRYAG 2 cut(s) 583, 906
BstSLI GKGCMC 1 cut(s) 951
BstV2I GAAGAC 1 cut(s) 989
BstX2I RGATCY 2 cut(s) 778, 1372
BstYI RGATCY 2 cut(s) 778, 1372
BsuI GTATCC 1 cut(s) 820
BsuRI GGCC 4 cut(s) 581, 1084, 1341, 1351
BtgZI GCGATG 2 cut(s) 112, 535
BtsCI GGATG 2 cut(s) 1307, 1365
BtsI GCAGTG 1 cut(s) 1300
BtsIMutI CAGTG 1 cut(s) 1300
Cac8I GCNNGC 2 cut(s) 269, 410
CaiI CAGNNNCTG 1 cut(s) 339
Cfr13I GGNCC 3 cut(s) 579, 1340, 1350
CseI GACGC 1 cut(s) 1028
Csp6I GTAC 1 cut(s) 884
CviAII CATG 3 cut(s) 21, 268, 409
CviQI GTAC 1 cut(s) 884
DdeI CTNAG 4 cut(s) 332, 524, 1176, 1211
DpnI GATC 4 cut(s) 116, 529, 780, 1374
DpnII GATC 4 cut(s) 114, 527, 778, 1372
DraI TTTAAA 1 cut(s) 1131
Eam1104I CTCTTC 8 cut(s) 66, 213, 299, 354, 440, 495, 636, 1176
EarI CTCTTC 8 cut(s) 66, 213, 299, 354, 440, 495, 636, 1176
Eco130I CCWWGG 4 cut(s) 319, 460, 601, 1329
Eco24I GRGCYC 5 cut(s) 192, 333, 474, 615, 756
Eco32I GATATC 1 cut(s) 27
Eco57I CTGAAG 6 cut(s) 54, 194, 335, 476, 617, 1361
Eco88I CYCGRG 1 cut(s) 1091
EcoRV GATATC 1 cut(s) 27
EcoT14I CCWWGG 4 cut(s) 319, 460, 601, 1329
EcoT38I GRGCYC 5 cut(s) 192, 333, 474, 615, 756
ErhI CCWWGG 4 cut(s) 319, 460, 601, 1329
Esp3I CGTCTC 2 cut(s) 279, 420
FaeI CATG 3 cut(s) 24, 271, 412
FaqI GGGAC 2 cut(s) 1173, 1290
FatI CATG 3 cut(s) 20, 267, 408
FauNDI CATATG 1 cut(s) 826
FokI GGATG 2 cut(s) 1314, 1352
FriOI GRGCYC 5 cut(s) 192, 333, 474, 615, 756
FspBI CTAG 8 cut(s) 9, 101, 179, 320, 461, 602, 806, 1163
GsuI CTGGAG 1 cut(s) 263
HaeIII GGCC 4 cut(s) 581, 1084, 1341, 1351
HapII CCGG 3 cut(s) 421, 845, 887
HgaI GACGC 1 cut(s) 1028
Hin1I GRCGYC 1 cut(s) 1020
Hin1II CATG 3 cut(s) 24, 271, 412
HinfI GANTC 8 cut(s) 104, 109, 289, 430, 911, 1094, 1205, 1231
HpaII CCGG 3 cut(s) 421, 845, 887
HphI GGTGA 2 cut(s) 956, 1264
Hpy188I TCNGA 4 cut(s) 114, 704, 992, 1212
HpyAV CCTTC 3 cut(s) 29, 46, 1033
HpyCH4III ACNGT 1 cut(s) 955
HpyCH4IV ACGT 1 cut(s) 226
HpyCH4V TGCA 6 cut(s) 201, 342, 483, 624, 765, 1326
HpyF3I CTNAG 4 cut(s) 332, 524, 1176, 1211
HpySE526I ACGT 1 cut(s) 226
Hsp92I GRCGYC 1 cut(s) 1020
Hsp92II CATG 3 cut(s) 24, 271, 412
Kpn2I TCCGGA 1 cut(s) 420
Kzo9I GATC 4 cut(s) 114, 527, 778, 1372
LguI GCTCTTC 1 cut(s) 1176
LmnI GCTCC 4 cut(s) 195, 759, 1012, 1293
LweI GCATC 7 cut(s) 135, 140, 558, 563, 699, 859, 1313
MaeI CTAG 8 cut(s) 9, 101, 179, 320, 461, 602, 806, 1163
MaeII ACGT 1 cut(s) 226
MaeIII GTNAC 4 cut(s) 92, 374, 515, 1295
MalI GATC 4 cut(s) 116, 529, 780, 1374
MboI GATC 4 cut(s) 114, 527, 778, 1372
MfeI CAATTG 2 cut(s) 88, 566
MflI RGATCY 2 cut(s) 778, 1372
MhlI GDGCHC 6 cut(s) 192, 333, 474, 615, 756, 951
MlyI GAGTC 3 cut(s) 113, 1103, 1214
MmeI TCCRAC 5 cut(s) 143, 198, 339, 707, 912
MroI TCCGGA 1 cut(s) 420
MroXI GAANNNNTTC 3 cut(s) 293, 434, 915
MseI TTAA 6 cut(s) 710, 737, 938, 1130, 1155, 1280
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 3 cut(s) 421, 845, 887
MspR9I CCNGG 1 cut(s) 845
MunI CAATTG 2 cut(s) 88, 566
NciI CCSGG 1 cut(s) 845
NdeI CATATG 1 cut(s) 826
NdeII GATC 4 cut(s) 114, 527, 778, 1372
NlaIII CATG 3 cut(s) 24, 271, 412
NlaIV GGNNCC 2 cut(s) 191, 755
NmuCI GTSAC 3 cut(s) 92, 374, 1295
NspI RCATGY 2 cut(s) 271, 412
PaeI GCATGC 2 cut(s) 271, 412
PaeR7I CTCGAG 1 cut(s) 1091
PciSI GCTCTTC 1 cut(s) 1176
PdmI GAANNNNTTC 3 cut(s) 293, 434, 915
PfeI GAWTC 5 cut(s) 109, 289, 430, 911, 1231
PleI GAGTC 3 cut(s) 112, 1102, 1213
PpsI GAGTC 3 cut(s) 112, 1102, 1213
Ppu21I YACGTR 1 cut(s) 227
PshAI GACNNNNGTC 2 cut(s) 90, 1302
PsiI TTATAA 1 cut(s) 788
PspN4I GGNNCC 2 cut(s) 191, 755
PspPI GGNCC 3 cut(s) 579, 1340, 1350
PspXI VCTCGAGB 1 cut(s) 1091
PstNI CAGNNNCTG 1 cut(s) 339
PsuI RGATCY 2 cut(s) 778, 1372
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 1 cut(s) 885
RsaNI GTAC 1 cut(s) 884
SapI GCTCTTC 1 cut(s) 1176
SaqAI TTAA 6 cut(s) 710, 737, 938, 1130, 1155, 1280
Sau3AI GATC 4 cut(s) 114, 527, 778, 1372
Sau96I GGNCC 3 cut(s) 579, 1340, 1350
SchI GAGTC 3 cut(s) 113, 1103, 1214
ScrFI CCNGG 1 cut(s) 845
SduI GDGCHC 6 cut(s) 192, 333, 474, 615, 756, 951
SfaNI GCATC 7 cut(s) 135, 140, 558, 563, 699, 859, 1313
SfcI CTRYAG 2 cut(s) 583, 906
Sfr274I CTCGAG 1 cut(s) 1091
SlaI CTCGAG 1 cut(s) 1091
SmlI CTYRAG 5 cut(s) 773, 858, 1091, 1202, 1235
SmoI CTYRAG 5 cut(s) 773, 858, 1091, 1202, 1235
SphI GCATGC 2 cut(s) 271, 412
SsiI CCGC 1 cut(s) 1291
SspMI CTAG 8 cut(s) 9, 101, 179, 320, 461, 602, 806, 1163
StyD4I CCNGG 1 cut(s) 843
StyI CCWWGG 4 cut(s) 319, 460, 601, 1329
TaaI ACNGT 1 cut(s) 955
TaiI ACGT 1 cut(s) 229
TaqI TCGA 5 cut(s) 69, 107, 596, 746, 1092
TfiI GAWTC 5 cut(s) 109, 289, 430, 911, 1231
Tru1I TTAA 6 cut(s) 710, 737, 938, 1130, 1155, 1280
Tru9I TTAA 6 cut(s) 710, 737, 938, 1130, 1155, 1280
TscAI CASTG 1 cut(s) 1300
TseFI GTSAC 3 cut(s) 92, 374, 1295
Tsp45I GTSAC 3 cut(s) 92, 374, 1295
TspDTI ATGAA 3 cut(s) 675, 1025, 1277
TspGWI ACGGA 3 cut(s) 6, 551, 1314
TspRI CASTG 1 cut(s) 1300
XapI RAATTY 3 cut(s) 852, 959, 1110
XceI RCATGY 2 cut(s) 271, 412
XhoI CTCGAG 1 cut(s) 1091
XmaJI CCTAGG 3 cut(s) 319, 460, 601
XmnI GAANNNNTTC 3 cut(s) 293, 434, 915
XspI CTAG 8 cut(s) 9, 101, 179, 320, 461, 602, 806, 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.