RchiOBHm_Chr5g0005081

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
N/A
Physical Location & Seq
Forward (+)
3313937 .. 3314880
944 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28631

Sequence Viewer

Length: 459 bp
ATGTTAATCCTGGAATACATGCCATACAGTAACTTGGACTCCTTTCTTTTTGATCATAGAAGAAGCACATCCTTAGATTGGAACGAGCGCCTTGAAACTATCAATGGGATTGCTCGCAGACTTCTTTATCTTCACCAAGACTCGAGACTGTGGATTATCCACAGAGATCTGAAAACCAGCAATGTTCTTCTAGATGCTGAAATGAACCCAAAAATTTCATATTTTGGCATGGCAAGAATATTCCATGGCGACCAACCCCTGGAGAAGACAAACAGAGTTGTTGGAACATATGGCTATATGTCACCAGAGTATGTTGTATTTGGAATTTTTTTCACAAAATCCGATGTCTTTAGTTTTGGGATCATACTATTGGAGATTCTAAGTGGCAAGAAAAGTAGCGGTCTTCAAGAGGATCATTCCATGAACTTGATAGGATATGTGAGTAACGAACCATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

152

Amino Acids

17.65

Weight (kDa)

5.92

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 1 - 132 2.4e-26 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 2 - 134 9.6e-25 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 259
AciI CCGC 1 cut(s) 399
AclWI GGATC 2 cut(s) 368, 420
AcsI RAATTY 2 cut(s) 213, 324
AfiI CCNNNNNNNGG 2 cut(s) 78, 259
AgsI TTSAA 2 cut(s) 95, 407
AjnI CCWGG 2 cut(s) 9, 258
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 2 cut(s) 368, 420
Ama87I CYCGRG 1 cut(s) 142
ApoI RAATTY 2 cut(s) 213, 324
AspLEI GCGC 1 cut(s) 90
AsuHPI GGTGA 2 cut(s) 125, 294
AvaI CYCGRG 1 cut(s) 142
BbsI GAAGAC 2 cut(s) 272, 395
BciT130I CCWGG 2 cut(s) 11, 260
BclI TGATCA 1 cut(s) 52
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 1 cut(s) 191
BfoI RGCGCY 1 cut(s) 91
BglII AGATCT 1 cut(s) 166
Bme1390I CCNGG 2 cut(s) 11, 260
BmeT110I CYCGRG 1 cut(s) 142
BmrFI CCNGG 2 cut(s) 11, 260
BmsI GCATC 1 cut(s) 184
BpiI GAAGAC 2 cut(s) 272, 395
BpmI CTGGAG 1 cut(s) 281
BsaJI CCNNGG 2 cut(s) 244, 258
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 259
Bse3DI GCAATG 1 cut(s) 187
BseBI CCWGG 2 cut(s) 11, 260
BseDI CCNNGG 2 cut(s) 244, 258
BseGI GGATG 1 cut(s) 68
BseLI CCNNNNNNNGG 2 cut(s) 78, 259
BseMI GCAATG 1 cut(s) 187
BsiHKCI CYCGRG 1 cut(s) 142
BslI CCNNNNNNNGG 2 cut(s) 78, 259
BsmAI GTCTC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 142
Bsp143I GATC 4 cut(s) 52, 166, 360, 412
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 1 cut(s) 399
BspPI GGATC 2 cut(s) 368, 420
BsrDI GCAATG 1 cut(s) 187
BssECI CCNNGG 2 cut(s) 244, 258
BssMI GATC 4 cut(s) 52, 166, 360, 412
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 2 cut(s) 11, 260
Bst4CI ACNGT 2 cut(s) 29, 150
BstC8I GCNNGC 1 cut(s) 115
BstDEI CTNAG 2 cut(s) 73, 380
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 1 cut(s) 68
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 4 cut(s) 55, 169, 363, 415
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 4 cut(s) 52, 166, 360, 412
BstNI CCWGG 2 cut(s) 11, 260
BstNSI RCATGY 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 9, 258
BstV2I GAAGAC 2 cut(s) 272, 395
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 1 cut(s) 68
Cac8I GCNNGC 1 cut(s) 115
CfoI GCGC 1 cut(s) 90
CviAII CATG 5 cut(s) 19, 229, 245, 421, 453
CviJI RGCY 1 cut(s) 294
CviKI_1 RGCY 1 cut(s) 294
DdeI CTNAG 2 cut(s) 73, 380
DpnI GATC 4 cut(s) 54, 168, 362, 414
DpnII GATC 4 cut(s) 52, 166, 360, 412
Eco130I CCWWGG 1 cut(s) 244
Eco88I CYCGRG 1 cut(s) 142
EcoRII CCWGG 2 cut(s) 9, 258
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 5 cut(s) 22, 232, 248, 424, 456
FatI CATG 5 cut(s) 18, 228, 244, 420, 452
FauNDI CATATG 1 cut(s) 289
FbaI TGATCA 1 cut(s) 52
FokI GGATG 1 cut(s) 55
FspBI CTAG 1 cut(s) 191
GlaI GCGC 1 cut(s) 89
GsuI CTGGAG 1 cut(s) 281
HaeII RGCGCY 1 cut(s) 91
HhaI GCGC 1 cut(s) 90
Hin1II CATG 5 cut(s) 22, 232, 248, 424, 456
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HinfI GANTC 3 cut(s) 38, 140, 376
HphI GGTGA 2 cut(s) 125, 294
Hpy188I TCNGA 2 cut(s) 171, 343
Hpy188III TCNNGA 3 cut(s) 144, 191, 407
HpyCH4III ACNGT 2 cut(s) 29, 150
HpyF3I CTNAG 2 cut(s) 73, 380
Hsp92II CATG 5 cut(s) 22, 232, 248, 424, 456
HspAI GCGC 1 cut(s) 88
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 4 cut(s) 52, 166, 360, 412
LpnPI CCDG 5 cut(s) 23, 190, 245, 272, 318
LweI GCATC 1 cut(s) 184
MaeI CTAG 1 cut(s) 191
MaeIII GTNAC 3 cut(s) 29, 300, 443
MalI GATC 4 cut(s) 54, 168, 362, 414
MboI GATC 4 cut(s) 52, 166, 360, 412
MboII GAAGA 5 cut(s) 72, 122, 179, 277, 395
MflI RGATCY 1 cut(s) 166
MluCI AATT 2 cut(s) 213, 324
MlyI GAGTC 2 cut(s) 32, 134
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 1 cut(s) 403
MseI TTAA 1 cut(s) 5
MspR9I CCNGG 2 cut(s) 11, 260
MvaI CCWGG 2 cut(s) 11, 260
NcoI CCATGG 1 cut(s) 244
NdeI CATATG 1 cut(s) 289
NdeII GATC 4 cut(s) 52, 166, 360, 412
NlaIII CATG 5 cut(s) 22, 232, 248, 424, 456
NmuCI GTSAC 1 cut(s) 300
NspI RCATGY 1 cut(s) 22
PaeR7I CTCGAG 1 cut(s) 142
PfeI GAWTC 1 cut(s) 376
PflMI CCANNNNNTGG 1 cut(s) 259
PfoI TCCNGGA 1 cut(s) 9
PleI GAGTC 2 cut(s) 32, 134
PpsI GAGTC 2 cut(s) 32, 134
Psp6I CCWGG 2 cut(s) 9, 258
PspGI CCWGG 2 cut(s) 9, 258
PsuI RGATCY 1 cut(s) 166
SaqAI TTAA 1 cut(s) 5
Sau3AI GATC 4 cut(s) 52, 166, 360, 412
SchI GAGTC 2 cut(s) 32, 134
ScrFI CCNGG 2 cut(s) 11, 260
SfaNI GCATC 1 cut(s) 184
Sfr274I CTCGAG 1 cut(s) 142
SlaI CTCGAG 1 cut(s) 142
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
Sse9I AATT 2 cut(s) 213, 324
SsiI CCGC 1 cut(s) 399
SspI AATATT 1 cut(s) 240
SspMI CTAG 1 cut(s) 191
StyD4I CCNGG 2 cut(s) 9, 258
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 2 cut(s) 29, 150
TaqI TCGA 1 cut(s) 143
TasI AATT 2 cut(s) 213, 324
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 1 cut(s) 5
Tru9I TTAA 1 cut(s) 5
TseFI GTSAC 1 cut(s) 300
Tsp45I GTSAC 1 cut(s) 300
TspDTI ATGAA 3 cut(s) 207, 218, 437
Van91I CCANNNNNTGG 1 cut(s) 259
XapI RAATTY 2 cut(s) 213, 324
XbaI TCTAGA 1 cut(s) 190
XceI RCATGY 1 cut(s) 22
XhoI CTCGAG 1 cut(s) 142
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.