RchiOBHm_Chr5g0064001

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
N/A
Physical Location & Seq
Forward (+)
69847777 .. 69848295
519 bp
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UTR
Exon/CDS
Intron
PRQ34007

Sequence Viewer

Length: 519 bp
ATGATTTTGAATCAACTAGACGGGCCTGGAATGACCTCATTAAGGCACGTACGGAAAGATGATGAAATCACTGCCATCGGGGTATATGGCATGGGAGGAGTTGGCAAGACAACTCTGGTGAAACATGTCAGCGCACAAGCTCGAGAACTTGGGCTTGTTAATTATGTGATTAGGGCTGTTGTAGGCCAGATCCCTGAATTGAGAAATATTCAAGGCACATTGGCTGATCAGCTGGGATTAAAATTCAAGAAGGAGGAGACAGAAATTCGAAGAGCCGCTAGATTGCTGGAGGGGATAATGAGAGGAAATAAGATCCTCATAGTCTTGGACAACGTTTGGCAGAGAATAAATTTGTCAAGAATAGGAATCCCCTCCTACAACGAGCTTCATGGATGCAATTCCAAACTCATACTAACCTCAACGAGAATGAATATTTGCTATTCCATGGAGTGCCAAGCAAGAATTCATCTCGATACCTTATCAGAAGAAGATTCTTGGAACCTTTTTGTGGAGAAATAG

Protein Analysis

172

Amino Acids

19.35

Weight (kDa)

8.82

Isoelectric Point (pI)

33.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 18 - 171 3.7e-25 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 276
AclI AACGTT 1 cut(s) 333
AclWI GGATC 2 cut(s) 184, 307
AcsI RAATTY 4 cut(s) 242, 264, 349, 462
AfaI GTAC 1 cut(s) 51
AfiI CCNNNNNNNGG 2 cut(s) 42, 508
AflIII ACRYGT 1 cut(s) 124
AgsI TTSAA 3 cut(s) 10, 212, 247
AjnI CCWGG 1 cut(s) 25
AjuI GAANNNNNNNTTGG 2 cut(s) 447, 479
AluBI AGCT 3 cut(s) 140, 232, 385
AluI AGCT 3 cut(s) 140, 232, 385
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 2 cut(s) 184, 307
Ama87I CYCGRG 1 cut(s) 141
AoxI GGCC 2 cut(s) 23, 184
ApoI RAATTY 4 cut(s) 242, 264, 349, 462
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 1 cut(s) 23
AsuHPI GGTGA 1 cut(s) 130
AsuII TTCGAA 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 141
BccI CCATC 1 cut(s) 83
BciT130I CCWGG 1 cut(s) 27
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 1 cut(s) 251
BfaI CTAG 2 cut(s) 17, 279
BisI GCNGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 277
Bme1390I CCNGG 1 cut(s) 27
BmeT110I CYCGRG 1 cut(s) 141
BmgT120I GGNCC 1 cut(s) 23
BmiI GGNNCC 1 cut(s) 500
BmrFI CCNGG 1 cut(s) 27
BmsI GCATC 1 cut(s) 383
BpmI CTGGAG 1 cut(s) 308
Bpu14I TTCGAA 1 cut(s) 268
BsaAI YACGTR 1 cut(s) 49
BsaJI CCNNGG 1 cut(s) 444
Bsc4I CCNNNNNNNGG 2 cut(s) 42, 508
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 444
BseGI GGATG 1 cut(s) 398
BseLI CCNNNNNNNGG 2 cut(s) 42, 508
BseRI GAGGAG 2 cut(s) 111, 269
BseYI CCCAGC 1 cut(s) 232
BshFI GGCC 2 cut(s) 25, 186
BsiHKCI CYCGRG 1 cut(s) 141
BsiWI CGTACG 1 cut(s) 49
BslI CCNNNNNNNGG 2 cut(s) 42, 508
BsmAI GTCTC 1 cut(s) 251
BsnI GGCC 2 cut(s) 25, 186
BsoBI CYCGRG 1 cut(s) 141
Bsp119I TTCGAA 1 cut(s) 268
Bsp143I GATC 3 cut(s) 189, 226, 312
Bsp19I CCATGG 1 cut(s) 444
BspACI CCGC 1 cut(s) 276
BspANI GGCC 2 cut(s) 25, 186
BspLI GGNNCC 1 cut(s) 500
BspPI GGATC 2 cut(s) 184, 307
BspQI GCTCTTC 1 cut(s) 265
BspT104I TTCGAA 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 444
BssMI GATC 3 cut(s) 189, 226, 312
BssT1I CCWWGG 1 cut(s) 444
Bst2UI CCWGG 1 cut(s) 27
Bst6I CTCTTC 1 cut(s) 265
BstBAI YACGTR 1 cut(s) 49
BstBI TTCGAA 1 cut(s) 268
BstDSI CCRYGG 1 cut(s) 444
BstENI CCTNNNNNAGG 1 cut(s) 40
BstF5I GGATG 1 cut(s) 398
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 3 cut(s) 192, 229, 315
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 3 cut(s) 189, 226, 312
BstNI CCWGG 1 cut(s) 27
BstNSI RCATGY 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 25
BstX2I RGATCY 2 cut(s) 189, 312
BstYI RGATCY 2 cut(s) 189, 312
BsuRI GGCC 2 cut(s) 25, 186
BtgI CCRYGG 1 cut(s) 444
BtsCI GGATG 1 cut(s) 398
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 1 cut(s) 69
CfoI GCGC 1 cut(s) 134
Cfr13I GGNCC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 50
CviAII CATG 4 cut(s) 91, 125, 389, 445
CviJI RGCY 9 cut(s) 25, 140, 154, 176, 186, 224, 232, 275, 385
CviKI_1 RGCY 9 cut(s) 25, 140, 154, 176, 186, 224, 232, 275, 385
CviQI GTAC 1 cut(s) 50
DpnI GATC 3 cut(s) 191, 228, 314
DpnII GATC 3 cut(s) 189, 226, 312
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 444
Eco88I CYCGRG 1 cut(s) 141
EcoNI CCTNNNNNAGG 1 cut(s) 40
EcoRI GAATTC 1 cut(s) 462
EcoRII CCWGG 1 cut(s) 25
EcoT14I CCWWGG 1 cut(s) 444
ErhI CCWWGG 1 cut(s) 444
FaeI CATG 4 cut(s) 94, 128, 392, 448
FaiI YATR 9 cut(s) 85, 87, 92, 126, 165, 320, 390, 410, 446
FatI CATG 4 cut(s) 90, 124, 388, 444
FbaI TGATCA 1 cut(s) 226
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 1 cut(s) 405
Fsp4HI GCNGC 1 cut(s) 276
FspBI CTAG 2 cut(s) 17, 279
GlaI GCGC 1 cut(s) 133
GluI GCNGC 1 cut(s) 276
GsaI CCCAGC 1 cut(s) 236
GsuI CTGGAG 1 cut(s) 308
HaeIII GGCC 2 cut(s) 25, 186
HhaI GCGC 1 cut(s) 134
Hin1II CATG 4 cut(s) 94, 128, 392, 448
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HinfI GANTC 3 cut(s) 10, 366, 491
HphI GGTGA 1 cut(s) 130
Hpy188I TCNGA 1 cut(s) 484
Hpy188III TCNNGA 4 cut(s) 143, 247, 357, 470
HpyAV CCTTC 1 cut(s) 244
HpyCH4IV ACGT 2 cut(s) 48, 333
HpyCH4V TGCA 1 cut(s) 396
HpySE526I ACGT 2 cut(s) 48, 333
Hsp92II CATG 4 cut(s) 94, 128, 392, 448
HspAI GCGC 1 cut(s) 132
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 3 cut(s) 189, 226, 312
LguI GCTCTTC 1 cut(s) 265
LpnPI CCDG 7 cut(s) 12, 39, 101, 200, 207, 218, 272
LweI GCATC 1 cut(s) 383
MaeI CTAG 2 cut(s) 17, 279
MaeII ACGT 2 cut(s) 48, 333
MalI GATC 3 cut(s) 191, 228, 314
MboI GATC 3 cut(s) 189, 226, 312
MboII GAAGA 3 cut(s) 282, 497, 500
MflI RGATCY 2 cut(s) 189, 312
MluCI AATT 7 cut(s) 160, 197, 242, 264, 349, 397, 462
MnlI CCTC 8 cut(s) 46, 89, 247, 283, 296, 326, 382, 427
MseI TTAA 3 cut(s) 41, 159, 239
MspA1I CMGCKG 1 cut(s) 232
MspR9I CCNGG 1 cut(s) 27
MvaI CCWGG 1 cut(s) 27
NcoI CCATGG 1 cut(s) 444
NdeII GATC 3 cut(s) 189, 226, 312
NlaIII CATG 4 cut(s) 94, 128, 392, 448
NlaIV GGNNCC 1 cut(s) 500
NspI RCATGY 1 cut(s) 128
NspV TTCGAA 1 cut(s) 268
PaeR7I CTCGAG 1 cut(s) 141
PciI ACATGT 1 cut(s) 124
PciSI GCTCTTC 1 cut(s) 265
PfeI GAWTC 3 cut(s) 10, 366, 491
Pfl23II CGTACG 1 cut(s) 49
PkrI GCNGC 1 cut(s) 277
Ppu21I YACGTR 1 cut(s) 49
PscI ACATGT 1 cut(s) 124
Psp1406I AACGTT 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 25
PspFI CCCAGC 1 cut(s) 232
PspGI CCWGG 1 cut(s) 25
PspLI CGTACG 1 cut(s) 49
PspN4I GGNNCC 1 cut(s) 500
PspPI GGNCC 1 cut(s) 23
PsuI RGATCY 2 cut(s) 189, 312
PvuII CAGCTG 1 cut(s) 232
RsaI GTAC 1 cut(s) 51
RsaNI GTAC 1 cut(s) 50
SapI GCTCTTC 1 cut(s) 265
SaqAI TTAA 3 cut(s) 41, 159, 239
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 3 cut(s) 189, 226, 312
Sau96I GGNCC 1 cut(s) 23
ScrFI CCNGG 1 cut(s) 27
SetI ASST 9 cut(s) 38, 51, 142, 234, 336, 387, 419, 479, 504
SfaNI GCATC 1 cut(s) 383
Sfr274I CTCGAG 1 cut(s) 141
SfuI TTCGAA 1 cut(s) 268
SlaI CTCGAG 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 141
SmoI CTYRAG 1 cut(s) 141
Sse9I AATT 7 cut(s) 160, 197, 242, 264, 349, 397, 462
SsiI CCGC 1 cut(s) 276
SspI AATATT 2 cut(s) 208, 433
SspMI CTAG 2 cut(s) 17, 279
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 1 cut(s) 444
TaiI ACGT 2 cut(s) 51, 336
TaqI TCGA 3 cut(s) 142, 268, 471
TasI AATT 7 cut(s) 160, 197, 242, 264, 349, 397, 462
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 3 cut(s) 10, 366, 491
Tru1I TTAA 3 cut(s) 41, 159, 239
Tru9I TTAA 3 cut(s) 41, 159, 239
TscAI CASTG 1 cut(s) 76
TspDTI ATGAA 4 cut(s) 78, 377, 443, 455
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 1 cut(s) 76
XagI CCTNNNNNAGG 1 cut(s) 40
XapI RAATTY 4 cut(s) 242, 264, 349, 462
XceI RCATGY 1 cut(s) 128
XhoI CTCGAG 1 cut(s) 141
XspI CTAG 2 cut(s) 17, 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.