RchiOBHm_Chr5g0070311
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
N/A
Physical Location & Seq
Forward (+)
76085805 .. 76086538
734 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34556

Sequence Viewer

Length: 399 bp
ATGGAGAATTCTCACTACAGTCAATCCCACACGGCTTCATGGATCAGAGGCAAGTGCGTAGGCAGAGGCGCGTTCGGCACGGTCAATATCGGATTCCGCAAATCGGACGGTGAAGTGTTCGCCGTCAAGTCCGTGGATCTCGCCGCGTGTCTCCCCAGCCAGGCGGAGGCGCTCGAGAACGAAATCCGGATTCTCCGGTCGCTCTCCTCGCCGTACGTCGTGAAGTACCTGAACGACGATCACACGGCGTCGTTTCGGAACTTGCATATGGAGTACTTGCCGGGCGGCACCGCCGGGGACTCTGCTGACGTGGACGAGAGTGTTGTACAGTCGCGGACGTGGTGCGTGGTGTCGGCTCTCAAGTATTTACACTCCCGGGGATTGTTCACTGCGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000186 GO:0000746 GO:0000747 GO:0000749 GO:0000750 GO:0000935 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007186 GO:0008150 GO:0008152 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010514 GO:0010562 GO:0010564 GO:0010604 GO:0010646 GO:0010647 GO:0010941 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019236 GO:0019538 GO:0019953 GO:0022414 GO:0022603 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030428 GO:0031098 GO:0031136 GO:0031137 GO:0031139 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032005 GO:0032147 GO:0032153 GO:0032155 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0034306 GO:0034307 GO:0035556 GO:0036211 GO:0042173 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043067 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0043902 GO:0043937 GO:0043940 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044703 GO:0044764 GO:0045595 GO:0045859 GO:0045860 GO:0045937 GO:0046999 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051286 GO:0051338 GO:0051347 GO:0051445 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0060284 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071444 GO:0071507 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0140096 GO:1901564 GO:1902531 GO:1902533 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

132

Amino Acids

14.46

Weight (kDa)

6.5

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 17 - 128 4.1e-17 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 287
AccII CGCG 3 cut(s) 71, 146, 334
AccIII TCCGGA 1 cut(s) 186
AciI CCGC 6 cut(s) 97, 144, 164, 285, 291, 334
AclWI GGATC 2 cut(s) 50, 144
AcsI RAATTY 1 cut(s) 7
AcyI GRCGYC 1 cut(s) 248
AfaI GTAC 4 cut(s) 215, 227, 275, 327
AfiI CCNNNNNNNGG 3 cut(s) 103, 160, 166
AjiI CACGTC 3 cut(s) 310, 339, 396
AjnI CCWGG 1 cut(s) 159
Alw26I GTCTC 1 cut(s) 155
AlwI GGATC 2 cut(s) 50, 144
Ama87I CYCGRG 2 cut(s) 173, 375
Aor13HI TCCGGA 1 cut(s) 186
ApoI RAATTY 1 cut(s) 7
AspLEI GCGC 2 cut(s) 71, 172
AsuC2I CCSGG 4 cut(s) 282, 295, 376, 377
AsuHPI GGTGA 1 cut(s) 122
AvaI CYCGRG 2 cut(s) 173, 375
BanI GGYRCC 1 cut(s) 287
BceAI ACGGC 4 cut(s) 48, 107, 196, 261
BciT130I CCWGG 1 cut(s) 161
BcnI CCSGG 4 cut(s) 282, 295, 376, 377
BcoDI GTCTC 1 cut(s) 155
BfmI CTRYAG 1 cut(s) 16
BfoI RGCGCY 1 cut(s) 173
BisI GCNGC 2 cut(s) 144, 286
BlsI GCNGC 2 cut(s) 145, 287
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 5 cut(s) 161, 282, 295, 376, 377
BmeT110I CYCGRG 2 cut(s) 173, 375
BmgBI CACGTC 3 cut(s) 310, 339, 396
BmiI GGNNCC 1 cut(s) 289
BmrFI CCNGG 5 cut(s) 161, 282, 295, 376, 377
BpuEI CTTGAG 1 cut(s) 344
BpuMI CCSGG 4 cut(s) 282, 295, 376, 377
BsaHI GRCGYC 1 cut(s) 248
BsaJI CCNNGG 4 cut(s) 132, 294, 375, 376
BsaWI WCCGGW 2 cut(s) 186, 195
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 160, 166
BseAI TCCGGA 1 cut(s) 186
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 4 cut(s) 132, 294, 375, 376
BseLI CCNNNNNNNGG 3 cut(s) 103, 160, 166
BseRI GAGGAG 1 cut(s) 196
BseYI CCCAGC 1 cut(s) 155
Bsh1236I CGCG 3 cut(s) 71, 146, 334
Bsh1285I CGRYCG 1 cut(s) 200
BshNI GGYRCC 1 cut(s) 287
BsiEI CGRYCG 1 cut(s) 200
BsiHKCI CYCGRG 2 cut(s) 173, 375
BsiSI CCGG 5 cut(s) 187, 196, 281, 294, 376
BsiWI CGTACG 1 cut(s) 213
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 3 cut(s) 103, 160, 166
BsmAI GTCTC 1 cut(s) 155
BsmFI GGGAC 1 cut(s) 311
BsoBI CYCGRG 2 cut(s) 173, 375
Bsp13I TCCGGA 1 cut(s) 186
Bsp1407I TGTACA 1 cut(s) 325
Bsp143I GATC 3 cut(s) 42, 136, 238
BspACI CCGC 6 cut(s) 97, 144, 164, 285, 291, 334
BspEI TCCGGA 1 cut(s) 186
BspFNI CGCG 3 cut(s) 71, 146, 334
BspLI GGNNCC 1 cut(s) 289
BspPI GGATC 2 cut(s) 50, 144
BspT107I GGYRCC 1 cut(s) 287
BsrGI TGTACA 1 cut(s) 325
BssECI CCNNGG 4 cut(s) 132, 294, 375, 376
BssMI GATC 3 cut(s) 42, 136, 238
BssNI GRCGYC 1 cut(s) 248
Bst2UI CCWGG 1 cut(s) 161
Bst4CI ACNGT 4 cut(s) 20, 82, 110, 330
BstACI GRCGYC 1 cut(s) 248
BstAUI TGTACA 1 cut(s) 325
BstDSI CCRYGG 1 cut(s) 132
BstFNI CGCG 3 cut(s) 71, 146, 334
BstH2I RGCGCY 1 cut(s) 173
BstHHI GCGC 2 cut(s) 71, 172
BstKTI GATC 3 cut(s) 45, 139, 241
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 3 cut(s) 42, 136, 238
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 2 cut(s) 75, 208
BstNI CCWGG 1 cut(s) 161
BstSCI CCNGG 5 cut(s) 159, 280, 293, 374, 375
BstSFI CTRYAG 1 cut(s) 16
BstUI CGCG 3 cut(s) 71, 146, 334
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BtgI CCRYGG 1 cut(s) 132
BtrI CACGTC 3 cut(s) 310, 339, 396
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 1 cut(s) 387
CfoI GCGC 2 cut(s) 71, 172
Cfr9I CCCGGG 1 cut(s) 375
CseI GACGC 1 cut(s) 237
Csp6I GTAC 4 cut(s) 214, 226, 274, 326
CviAII CATG 1 cut(s) 39
CviJI RGCY 3 cut(s) 35, 159, 356
CviKI_1 RGCY 3 cut(s) 35, 159, 356
CviQI GTAC 4 cut(s) 214, 226, 274, 326
DpnI GATC 3 cut(s) 44, 138, 240
DpnII GATC 3 cut(s) 42, 136, 238
EciI GGCGGA 1 cut(s) 179
Eco88I CYCGRG 2 cut(s) 173, 375
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 159
FaeI CATG 1 cut(s) 42
FaiI YATR 3 cut(s) 40, 267, 269
FaqI GGGAC 1 cut(s) 311
FatI CATG 1 cut(s) 38
FauNDI CATATG 1 cut(s) 267
Fnu4HI GCNGC 2 cut(s) 144, 286
Fsp4HI GCNGC 2 cut(s) 144, 286
GlaI GCGC 2 cut(s) 70, 171
GluI GCNGC 2 cut(s) 144, 286
GsaI CCCAGC 1 cut(s) 159
HaeII RGCGCY 1 cut(s) 173
HapII CCGG 5 cut(s) 187, 196, 281, 294, 376
HgaI GACGC 1 cut(s) 237
HhaI GCGC 2 cut(s) 71, 172
Hin1I GRCGYC 1 cut(s) 248
Hin1II CATG 1 cut(s) 42
Hin6I GCGC 2 cut(s) 69, 170
HinP1I GCGC 2 cut(s) 69, 170
HinfI GANTC 3 cut(s) 93, 190, 299
HpaII CCGG 5 cut(s) 187, 196, 281, 294, 376
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 2 cut(s) 313, 387
Hpy188I TCNGA 4 cut(s) 47, 92, 106, 258
Hpy188III TCNNGA 3 cut(s) 175, 187, 220
Hpy8I GTNNAC 2 cut(s) 313, 387
Hpy99I CGWCG 4 cut(s) 221, 239, 253, 397
HpyCH4III ACNGT 4 cut(s) 20, 82, 110, 330
HpyCH4IV ACGT 4 cut(s) 216, 309, 338, 395
HpyCH4V TGCA 1 cut(s) 265
HpyF10VI GCNNNNNNNGC 2 cut(s) 75, 208
HpySE526I ACGT 4 cut(s) 216, 309, 338, 395
Hsp92I GRCGYC 1 cut(s) 248
Hsp92II CATG 1 cut(s) 42
HspAI GCGC 2 cut(s) 69, 170
Kpn2I TCCGGA 1 cut(s) 186
Kzo9I GATC 3 cut(s) 42, 136, 238
LpnPI CCDG 9 cut(s) 146, 169, 173, 200, 209, 242, 294, 307, 389
MaeII ACGT 4 cut(s) 216, 309, 338, 395
MalI GATC 3 cut(s) 44, 138, 240
MboI GATC 3 cut(s) 42, 136, 238
MflI RGATCY 1 cut(s) 136
MluCI AATT 1 cut(s) 7
MlyI GAGTC 1 cut(s) 293
MnlI CCTC 4 cut(s) 41, 59, 160, 217
MroI TCCGGA 1 cut(s) 186
MspI CCGG 5 cut(s) 187, 196, 281, 294, 376
MspR9I CCNGG 5 cut(s) 161, 282, 295, 376, 377
MvaI CCWGG 1 cut(s) 161
MvnI CGCG 3 cut(s) 71, 146, 334
MwoI GCNNNNNNNGC 2 cut(s) 75, 208
NciI CCSGG 4 cut(s) 282, 295, 376, 377
NdeI CATATG 1 cut(s) 267
NdeII GATC 3 cut(s) 42, 136, 238
NlaIII CATG 1 cut(s) 42
NlaIV GGNNCC 1 cut(s) 289
PaeR7I CTCGAG 1 cut(s) 173
PfeI GAWTC 2 cut(s) 93, 190
Pfl23II CGTACG 1 cut(s) 213
PkrI GCNGC 2 cut(s) 145, 287
PleI GAGTC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 293
Psp6I CCWGG 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 155
PspGI CCWGG 1 cut(s) 159
PspLI CGTACG 1 cut(s) 213
PspN4I GGNNCC 1 cut(s) 289
PsuI RGATCY 1 cut(s) 136
RsaI GTAC 4 cut(s) 215, 227, 275, 327
RsaNI GTAC 4 cut(s) 214, 226, 274, 326
SatI GCNGC 2 cut(s) 144, 286
Sau3AI GATC 3 cut(s) 42, 136, 238
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 5 cut(s) 161, 282, 295, 376, 377
SetI ASST 5 cut(s) 219, 231, 312, 341, 398
SfcI CTRYAG 1 cut(s) 16
Sfr274I CTCGAG 1 cut(s) 173
SlaI CTCGAG 1 cut(s) 173
SmaI CCCGGG 1 cut(s) 377
SmlI CTYRAG 2 cut(s) 173, 359
SmoI CTYRAG 2 cut(s) 173, 359
Sse9I AATT 1 cut(s) 7
SsiI CCGC 6 cut(s) 97, 144, 164, 285, 291, 334
StyD4I CCNGG 5 cut(s) 159, 280, 293, 374, 375
TaaI ACNGT 4 cut(s) 20, 82, 110, 330
TaiI ACGT 4 cut(s) 219, 312, 341, 398
TaqI TCGA 1 cut(s) 174
TasI AATT 1 cut(s) 7
TatI WGTACW 2 cut(s) 273, 325
TauI GCSGC 2 cut(s) 146, 288
TfiI GAWTC 2 cut(s) 93, 190
TscAI CASTG 1 cut(s) 394
TspDTI ATGAA 1 cut(s) 27
TspGWI ACGGA 1 cut(s) 121
TspMI CCCGGG 1 cut(s) 375
TspRI CASTG 1 cut(s) 394
XapI RAATTY 1 cut(s) 7
XhoI CTCGAG 1 cut(s) 173
XmaI CCCGGG 1 cut(s) 375
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.