RLG00000003251

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
N/A
Physical Location & Seq
Reverse (-)
46117857 .. 46132609
14753 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003251

Sequence Viewer

Length: 915 bp
ATGGGCAGAGCAATTGTTCAGTCAAAATCCCCTCAGAACCCTGGGAAACGTTGTAGATTGTGGGATGATGCAGATGCTACAGATGTATTGAGGGATAAATCTACTGTACAAGTTTGTCAGAGGTTCATCAGTCCGTTGGACATCTTGATAGACTTTCTGCGATTTCAGAAGTTGGATGACGACTTGGGGAATATGGAATCATTGATTACGCTGGATGCTGGTAACACAGCGATAAGAGAAATACCATCTTCCATTGTGAGATTGAAGAACTTGTGTGTCACCTGTGGAGGTGAAGAAAATAGGGTTCAAGCTGGGTGCAACAGCCCAACCTGGGCTGTAGCGCTTGTGTTGCTTAAGAAGTTGAAGATTCTTAATCTTAGTCATTCCCATTACTTATCACATTTACCGGACCTTTCAAGACTCCCAAATCTTGAGAATCTTGTTCTTGAAGACTGTACAAATTTGTCAGAGGTTCATCCGTCCGTTGGACACCTTGATAGACTTTCTGCGGTAAATCTCAAAGGCTGCATTATGCTTAGGGATCTTCCAAGGAGTTTCTATATGATAAAATCAATCACAACTCTTGTTCTTGCTGACTGTTCAAGATTTCACAAGTTGGATGACGACTTGGGGAATATGGAATCATTGATTACGCTGGTTGCTGGTAACACAACGATAAGAGAAATACCATCTTCCATTGTGAGATTGAAGAACTTGGTACGTGTATCACCCGCGAAGGTGAAGAAAATTGGGTTCAAGCTGGTATGGAGTGACAAATTAGCAGATTTCAGGGGATTTGATGATGATCAGCCTAGTCCGTGTTCTCTCCCATATAGTTCATTACCAACATATAGCAGGATGCTTAGTAGATCATTATCTGCATTTGTAAGTGCATTTGCAAGAGTATGTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04621 ko04626 map04621 map04626
Pfam Domains
Protein Families
AP2 CS NB-ARC SGS TIR TPR_16 TPR_2 TPR_8

Protein Analysis

305

Amino Acids

33.84

Weight (kDa)

8.66

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 55 - 145 4.7e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 734
AciI CCGC 2 cut(s) 509, 732
AclI AACGTT 1 cut(s) 49
AclWI GGATC 1 cut(s) 549
AcsI RAATTY 1 cut(s) 460
AfaI GTAC 3 cut(s) 108, 457, 720
AfeI AGCGCT 1 cut(s) 342
AfiI CCNNNNNNNGG 2 cut(s) 331, 485
AflII CTTAAG 1 cut(s) 353
AflIII ACRYGT 1 cut(s) 721
AgsI TTSAA 8 cut(s) 265, 308, 364, 417, 449, 603, 709, 757
AjnI CCWGG 2 cut(s) 40, 329
AluBI AGCT 2 cut(s) 311, 760
AluI AGCT 2 cut(s) 311, 760
AlwI GGATC 1 cut(s) 549
Aor51HI AGCGCT 1 cut(s) 342
ApeKI GCWGC 1 cut(s) 525
ApoI RAATTY 1 cut(s) 460
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 1 cut(s) 409
AsuHPI GGTGA 4 cut(s) 271, 302, 720, 751
AvaII GGWCC 1 cut(s) 409
BbsI GAAGAC 1 cut(s) 456
BbvI GCAGC 1 cut(s) 512
BccI CCATC 2 cut(s) 253, 697
BciT130I CCWGG 2 cut(s) 42, 331
BclI TGATCA 1 cut(s) 805
BfaI CTAG 1 cut(s) 813
BfmI CTRYAG 2 cut(s) 78, 336
BfoI RGCGCY 1 cut(s) 344
BfrI CTTAAG 1 cut(s) 353
BisI GCNGC 1 cut(s) 526
BlsI GCNGC 1 cut(s) 527
Bme1390I CCNGG 2 cut(s) 42, 331
Bme18I GGWCC 1 cut(s) 409
BmgT120I GGNCC 1 cut(s) 409
BmrFI CCNGG 2 cut(s) 42, 331
BmsI GCATC 4 cut(s) 58, 64, 205, 849
BpiI GAAGAC 1 cut(s) 456
Bpu10I CCTNAGC 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 452
BsaAI YACGTR 1 cut(s) 722
BsaBI GATNNNNATC 2 cut(s) 804, 874
BsaJI CCNNGG 4 cut(s) 40, 41, 330, 548
BsaWI WCCGGW 1 cut(s) 406
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 485
Bse8I GATNNNNATC 2 cut(s) 804, 874
BseBI CCWGG 2 cut(s) 42, 331
BseDI CCNNGG 4 cut(s) 40, 41, 330, 548
BseGI GGATG 6 cut(s) 70, 181, 220, 475, 625, 864
BseJI GATNNNNATC 2 cut(s) 804, 874
BseLI CCNNNNNNNGG 2 cut(s) 331, 485
BseMII CTCAG 1 cut(s) 47
BseXI GCAGC 1 cut(s) 512
BseYI CCCAGC 1 cut(s) 311
Bsh1236I CGCG 1 cut(s) 734
BsiSI CCGG 1 cut(s) 407
BslI CCNNNNNNNGG 2 cut(s) 331, 485
Bsp1407I TGTACA 2 cut(s) 106, 455
Bsp143I GATC 3 cut(s) 541, 805, 869
BspACI CCGC 2 cut(s) 509, 732
BspCNI CTCAG 1 cut(s) 46
BspFNI CGCG 1 cut(s) 734
BspPI GGATC 1 cut(s) 549
BspTI CTTAAG 1 cut(s) 353
BsrGI TGTACA 2 cut(s) 106, 455
BssECI CCNNGG 4 cut(s) 40, 41, 330, 548
BssMI GATC 3 cut(s) 541, 805, 869
BssT1I CCWWGG 1 cut(s) 548
Bst2UI CCWGG 2 cut(s) 42, 331
Bst4CI ACNGT 3 cut(s) 106, 455, 599
BstAFI CTTAAG 1 cut(s) 353
BstAUI TGTACA 2 cut(s) 106, 455
BstBAI YACGTR 1 cut(s) 722
BstDEI CTNAG 4 cut(s) 33, 377, 536, 863
BstF5I GGATG 6 cut(s) 70, 181, 220, 475, 625, 864
BstFNI CGCG 1 cut(s) 734
BstH2I RGCGCY 1 cut(s) 344
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 3 cut(s) 544, 808, 872
BstMBI GATC 3 cut(s) 541, 805, 869
BstMWI GCNNNNNNNGC 1 cut(s) 349
BstNI CCWGG 2 cut(s) 42, 331
BstSCI CCNGG 2 cut(s) 40, 329
BstSFI CTRYAG 2 cut(s) 78, 336
BstUI CGCG 1 cut(s) 734
BstV1I GCAGC 1 cut(s) 512
BstV2I GAAGAC 1 cut(s) 456
BstX2I RGATCY 1 cut(s) 541
BstYI RGATCY 1 cut(s) 541
BtsCI GGATG 6 cut(s) 70, 181, 220, 475, 625, 864
CfoI GCGC 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 409
Csp6I GTAC 3 cut(s) 107, 456, 719
CviJI RGCY 6 cut(s) 311, 324, 335, 525, 760, 811
CviKI_1 RGCY 6 cut(s) 311, 324, 335, 525, 760, 811
CviQI GTAC 3 cut(s) 107, 456, 719
DdeI CTNAG 4 cut(s) 33, 377, 536, 863
DpnI GATC 3 cut(s) 543, 807, 871
DpnII GATC 3 cut(s) 541, 805, 869
Eco130I CCWWGG 1 cut(s) 548
Eco47I GGWCC 1 cut(s) 409
Eco47III AGCGCT 1 cut(s) 342
EcoRII CCWGG 2 cut(s) 40, 329
EcoT14I CCWWGG 1 cut(s) 548
ErhI CCWWGG 1 cut(s) 548
FauI CCCGC 1 cut(s) 739
FbaI TGATCA 1 cut(s) 805
Fnu4HI GCNGC 1 cut(s) 526
FokI GGATG 6 cut(s) 77, 188, 227, 462, 632, 871
Fsp4HI GCNGC 1 cut(s) 526
FspBI CTAG 1 cut(s) 813
GlaI GCGC 1 cut(s) 342
GluI GCNGC 1 cut(s) 526
GsaI CCCAGC 1 cut(s) 315
HaeII RGCGCY 1 cut(s) 344
HapII CCGG 1 cut(s) 407
HhaI GCGC 1 cut(s) 343
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HinfI GANTC 5 cut(s) 197, 367, 420, 436, 641
HpaII CCGG 1 cut(s) 407
HphI GGTGA 4 cut(s) 271, 302, 720, 751
Hpy188I TCNGA 4 cut(s) 36, 120, 168, 469
Hpy188III TCNNGA 5 cut(s) 145, 417, 431, 446, 603
HpyAV CCTTC 1 cut(s) 730
HpyCH4III ACNGT 3 cut(s) 106, 455, 599
HpyCH4IV ACGT 2 cut(s) 49, 721
HpyCH4V TGCA 6 cut(s) 71, 318, 528, 881, 893, 899
HpyF10VI GCNNNNNNNGC 1 cut(s) 349
HpyF3I CTNAG 4 cut(s) 33, 377, 536, 863
HpySE526I ACGT 2 cut(s) 49, 721
HspAI GCGC 1 cut(s) 341
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 3 cut(s) 541, 805, 869
Lsp1109I GCAGC 1 cut(s) 512
LweI GCATC 4 cut(s) 58, 64, 205, 849
MaeI CTAG 1 cut(s) 813
MaeII ACGT 2 cut(s) 49, 721
MaeIII GTNAC 4 cut(s) 221, 277, 665, 770
MalI GATC 3 cut(s) 543, 807, 871
MboI GATC 3 cut(s) 541, 805, 869
MboII GAAGA 9 cut(s) 240, 277, 305, 376, 461, 536, 684, 721, 754
MfeI CAATTG 1 cut(s) 12
MflI RGATCY 1 cut(s) 541
MluCI AATT 4 cut(s) 12, 460, 747, 776
MlyI GAGTC 1 cut(s) 414
MmeI TCCRAC 4 cut(s) 117, 153, 466, 597
MnlI CCTC 5 cut(s) 42, 84, 114, 281, 463
MseI TTAA 2 cut(s) 354, 372
MspCI CTTAAG 1 cut(s) 353
MspI CCGG 1 cut(s) 407
MspR9I CCNGG 2 cut(s) 42, 331
MunI CAATTG 1 cut(s) 12
MvaI CCWGG 2 cut(s) 42, 331
MvnI CGCG 1 cut(s) 734
MwoI GCNNNNNNNGC 1 cut(s) 349
NdeII GATC 3 cut(s) 541, 805, 869
NmuCI GTSAC 2 cut(s) 277, 770
PasI CCCWGGG 1 cut(s) 41
PfeI GAWTC 4 cut(s) 197, 367, 436, 641
PkrI GCNGC 1 cut(s) 527
PleI GAGTC 1 cut(s) 414
PpsI GAGTC 1 cut(s) 414
Ppu21I YACGTR 1 cut(s) 722
Psp1406I AACGTT 1 cut(s) 49
Psp6I CCWGG 2 cut(s) 40, 329
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 2 cut(s) 40, 329
PspPI GGNCC 1 cut(s) 409
PsuI RGATCY 1 cut(s) 541
RsaI GTAC 3 cut(s) 108, 457, 720
RsaNI GTAC 3 cut(s) 107, 456, 719
SaqAI TTAA 2 cut(s) 354, 372
SatI GCNGC 1 cut(s) 526
Sau3AI GATC 3 cut(s) 541, 805, 869
Sau96I GGNCC 1 cut(s) 409
SchI GAGTC 1 cut(s) 414
ScrFI CCNGG 2 cut(s) 42, 331
SfaNI GCATC 4 cut(s) 58, 64, 205, 849
SfcI CTRYAG 2 cut(s) 78, 336
SinI GGWCC 1 cut(s) 409
SmlI CTYRAG 2 cut(s) 353, 431
SmoI CTYRAG 2 cut(s) 353, 431
Sse9I AATT 4 cut(s) 12, 460, 747, 776
SsiI CCGC 2 cut(s) 509, 732
SspMI CTAG 1 cut(s) 813
StyD4I CCNGG 2 cut(s) 40, 329
StyI CCWWGG 1 cut(s) 548
TaaI ACNGT 3 cut(s) 106, 455, 599
TaiI ACGT 2 cut(s) 52, 724
TasI AATT 4 cut(s) 12, 460, 747, 776
TatI WGTACW 2 cut(s) 106, 455
TfiI GAWTC 4 cut(s) 197, 367, 436, 641
Tru1I TTAA 2 cut(s) 354, 372
Tru9I TTAA 2 cut(s) 354, 372
TseFI GTSAC 2 cut(s) 277, 770
TseI GCWGC 1 cut(s) 525
Tsp45I GTSAC 2 cut(s) 277, 770
TspDTI ATGAA 3 cut(s) 115, 464, 828
TspGWI ACGGA 4 cut(s) 123, 468, 472, 807
Vha464I CTTAAG 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 409
XapI RAATTY 1 cut(s) 460
XspI CTAG 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.