RLG00000010158

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
N/A
Physical Location & Seq
Forward (+)
63664664 .. 63665502
839 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010158

Sequence Viewer

Length: 411 bp
ATGATTTCCAGTCTCGATTGGAAGCTTGCCGGCGGTGTCATGCTGACGACGTCTTCTCCATCAAAGGACGTCAGTCCTCGAGCTGCTAACCGTGGCATTCTCCTTGCTGATTCCCAGTCTGATGAGGCATGTTCTGAGGACTCTGAGTTTGTTGTTGTTTTTCCTGGAAGTGAGGTTCCAAAGTGCTTCGACTCTCAATTAATGAGGGAAAACGAAGGCTTTGCTCTTTGTGTTGTTTATGAAGAAGTACCGGGAATTAATTTAGATAGGTCGTCATTTGGTTTTGGAGTTTCTGTTCATATGGATTATGTAGACATAGATCGTCACCGGTTTTCTACGTCTGCACAAGAGAGTACACCTTCAGCTCATGTGTGGCTAAAGTGTATTCCATTAACGGTTGACATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

137

Amino Acids

14.89

Weight (kDa)

4.61

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 53, 72
AccI GTMKAC 1 cut(s) 312
AciI CCGC 1 cut(s) 33
AcuI CTGAAG 1 cut(s) 345
AcyI GRCGYC 2 cut(s) 50, 69
AfaI GTAC 2 cut(s) 249, 355
AgeI ACCGGT 1 cut(s) 327
AjnI CCWGG 1 cut(s) 163
AloI GAACNNNNNNTCC 4 cut(s) 159, 191, 279, 311
AluBI AGCT 3 cut(s) 25, 83, 365
AluI AGCT 3 cut(s) 25, 83, 365
Alw26I GTCTC 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 78
ApeKI GCWGC 1 cut(s) 83
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AseI ATTAAT 2 cut(s) 200, 258
AsiGI ACCGGT 1 cut(s) 327
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 317
AvaI CYCGRG 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 70
BccI CCATC 1 cut(s) 67
BcgI CGANNNNNNTGC 2 cut(s) 203, 237
BciT130I CCWGG 1 cut(s) 165
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 17
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 2 cut(s) 165, 252
BmeT110I CYCGRG 1 cut(s) 78
BmiI GGNNCC 1 cut(s) 177
BmrFI CCNGG 2 cut(s) 165, 252
BmrI ACTGGG 1 cut(s) 109
BmuI ACTGGG 1 cut(s) 109
BoxI GACNNNNGTC 1 cut(s) 72
BpiI GAAGAC 1 cut(s) 45
BpuMI CCSGG 1 cut(s) 252
BsaHI GRCGYC 2 cut(s) 50, 69
BsaJI CCNNGG 1 cut(s) 91
BsaWI WCCGGW 1 cut(s) 327
BsaXI ACNNNNNCTCC 4 cut(s) 40, 70, 279, 309
Bse118I RCCGGY 2 cut(s) 29, 327
Bse1I ACTGG 2 cut(s) 9, 115
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 1 cut(s) 91
BseMII CTCAG 2 cut(s) 126, 135
BseNI ACTGG 2 cut(s) 9, 115
BseXI GCAGC 1 cut(s) 70
BsgI GTGCAG 1 cut(s) 327
BshTI ACCGGT 1 cut(s) 327
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 3 cut(s) 30, 251, 328
BsmAI GTCTC 1 cut(s) 17
BsmI GAATGC 1 cut(s) 96
BsoBI CYCGRG 1 cut(s) 78
Bsp143I GATC 1 cut(s) 319
BspACI CCGC 1 cut(s) 33
BspCNI CTCAG 2 cut(s) 127, 136
BspLI GGNNCC 1 cut(s) 177
BsrFI RCCGGY 2 cut(s) 29, 327
BsrI ACTGG 2 cut(s) 9, 115
BssAI RCCGGY 2 cut(s) 29, 327
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 1 cut(s) 319
BssNI GRCGYC 2 cut(s) 50, 69
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 92, 397
BstACI GRCGYC 2 cut(s) 50, 69
BstC8I GCNNGC 2 cut(s) 27, 31
BstDEI CTNAG 2 cut(s) 135, 144
BstDSI CCRYGG 1 cut(s) 91
BstKTI GATC 1 cut(s) 322
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 1 cut(s) 319
BstNI CCWGG 1 cut(s) 165
BstNSI RCATGY 1 cut(s) 132
BstPAI GACNNNNGTC 1 cut(s) 72
BstSCI CCNGG 2 cut(s) 163, 250
BstV1I GCAGC 1 cut(s) 70
BstV2I GAAGAC 1 cut(s) 45
BtgI CCRYGG 1 cut(s) 91
Cac8I GCNNGC 2 cut(s) 27, 31
Cfr10I RCCGGY 2 cut(s) 29, 327
Csp6I GTAC 2 cut(s) 248, 354
CspAI ACCGGT 1 cut(s) 327
CviAII CATG 3 cut(s) 40, 129, 368
CviJI RGCY 5 cut(s) 25, 83, 219, 365, 376
CviKI_1 RGCY 5 cut(s) 25, 83, 219, 365, 376
CviQI GTAC 2 cut(s) 248, 354
DdeI CTNAG 2 cut(s) 135, 144
DpnI GATC 1 cut(s) 321
DpnII GATC 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 345
Eco88I CYCGRG 1 cut(s) 78
EcoRII CCWGG 1 cut(s) 163
FaeI CATG 3 cut(s) 43, 132, 371
FaiI YATR 8 cut(s) 41, 130, 240, 300, 302, 309, 317, 369
FatI CATG 3 cut(s) 39, 128, 367
FauNDI CATATG 1 cut(s) 300
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 1 cut(s) 84
Fsp4HI GCNGC 1 cut(s) 84
GluI GCNGC 1 cut(s) 84
HapII CCGG 3 cut(s) 30, 251, 328
Hin1I GRCGYC 2 cut(s) 50, 69
Hin1II CATG 3 cut(s) 43, 132, 371
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HindIII AAGCTT 1 cut(s) 23
HinfI GANTC 3 cut(s) 110, 140, 191
HpaII CCGG 3 cut(s) 30, 251, 328
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 3 cut(s) 313, 356, 400
Hpy188I TCNGA 3 cut(s) 121, 136, 145
Hpy188III TCNNGA 1 cut(s) 14
Hpy8I GTNNAC 3 cut(s) 313, 356, 400
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 2 cut(s) 209, 369
HpyCH4III ACNGT 2 cut(s) 92, 397
HpyCH4IV ACGT 3 cut(s) 50, 69, 338
HpyCH4V TGCA 1 cut(s) 344
HpyF3I CTNAG 2 cut(s) 135, 144
HpySE526I ACGT 3 cut(s) 50, 69, 338
Hsp92I GRCGYC 2 cut(s) 50, 69
Hsp92II CATG 3 cut(s) 43, 132, 371
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 1 cut(s) 319
LpnPI CCDG 7 cut(s) 22, 43, 128, 150, 177, 264, 341
Lsp1109I GCAGC 1 cut(s) 70
MaeII ACGT 3 cut(s) 50, 69, 338
MaeIII GTNAC 1 cut(s) 323
MalI GATC 1 cut(s) 321
MboI GATC 1 cut(s) 319
MboII GAAGA 2 cut(s) 45, 254
MluCI AATT 3 cut(s) 197, 255, 259
MlyI GAGTC 2 cut(s) 134, 185
MnlI CCTC 5 cut(s) 87, 118, 130, 166, 198
MroNI GCCGGC 1 cut(s) 29
MseI TTAA 3 cut(s) 200, 258, 392
MspI CCGG 3 cut(s) 30, 251, 328
MspR9I CCNGG 2 cut(s) 165, 252
Mva1269I GAATGC 1 cut(s) 96
MvaI CCWGG 1 cut(s) 165
NaeI GCCGGC 1 cut(s) 31
NciI CCSGG 1 cut(s) 252
NdeI CATATG 1 cut(s) 300
NdeII GATC 1 cut(s) 319
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 3 cut(s) 43, 132, 371
NlaIV GGNNCC 1 cut(s) 177
NmuCI GTSAC 1 cut(s) 323
NspI RCATGY 1 cut(s) 132
PaeR7I CTCGAG 1 cut(s) 78
PctI GAATGC 1 cut(s) 96
PdiI GCCGGC 1 cut(s) 31
PfeI GAWTC 1 cut(s) 110
PflFI GACNNNGTC 1 cut(s) 49
PfoI TCCNGGA 1 cut(s) 163
PinAI ACCGGT 1 cut(s) 327
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 2 cut(s) 134, 185
PpsI GAGTC 2 cut(s) 134, 185
PshAI GACNNNNGTC 1 cut(s) 72
PshBI ATTAAT 2 cut(s) 200, 258
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 177
PspXI VCTCGAGB 1 cut(s) 78
PsyI GACNNNGTC 1 cut(s) 49
RsaI GTAC 2 cut(s) 249, 355
RsaNI GTAC 2 cut(s) 248, 354
SaqAI TTAA 3 cut(s) 200, 258, 392
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 1 cut(s) 319
SchI GAGTC 2 cut(s) 134, 185
ScrFI CCNGG 2 cut(s) 165, 252
SetI ASST 9 cut(s) 27, 53, 72, 85, 177, 272, 341, 361, 367
Sfr274I CTCGAG 1 cut(s) 78
SlaI CTCGAG 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
Sse9I AATT 3 cut(s) 197, 255, 259
SsiI CCGC 1 cut(s) 33
StyD4I CCNGG 2 cut(s) 163, 250
TaaI ACNGT 2 cut(s) 92, 397
TaiI ACGT 3 cut(s) 53, 72, 341
TaqI TCGA 3 cut(s) 15, 79, 189
TasI AATT 3 cut(s) 197, 255, 259
TatI WGTACW 1 cut(s) 353
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 3 cut(s) 200, 258, 392
Tru9I TTAA 3 cut(s) 200, 258, 392
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 2 cut(s) 255, 287
Tth111I GACNNNGTC 1 cut(s) 49
VspI ATTAAT 2 cut(s) 200, 258
XceI RCATGY 1 cut(s) 132
XhoI CTCGAG 1 cut(s) 78
XmiI GTMKAC 1 cut(s) 312
ZraI GACGTC 2 cut(s) 51, 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.