RLG00000015722

Non-specific serine threonine protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
N/A
Physical Location & Seq
Forward (+)
984941 .. 985430
490 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015722

Sequence Viewer

Length: 393 bp
ATGTATACAGCCCGAAATGGTAAAATCCCCAATGAGGGCAGTATCGTAACAACGCACCAACGTGCCGATGCGCTGTGTCCGAAAACCAAGGGGAACCCCACACGTGTCCAAAACCGTGACGTCACAACCAACGTCAAAGACGTCCCGTCTTTTGACACAAGGCTATGCACTGGAGGCAAAAGAAAAAACGCAGAGAGATCAACAGAGCACTGCTGCTCGGTGGTCCTTCTTAAGCAAAAAGGGTTCCAAAATTCTAAGCGAGTATCAATTAAGGGTTTTAGGGAATGCAGAGAGATGATAGGAGCTTCGTTTAGAAAGCCTGAGCTGTGTGTTAGAGCCAAACGCTGCATCGGCGGCAGAAACTGTCACCGGAAACACGTAGAAGACGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAF Pkinase

Protein Analysis

131

Amino Acids

14.6

Weight (kDa)

10.06

Isoelectric Point (pI)

29.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 123, 144
AccI GTMKAC 1 cut(s) 5
AciI CCGC 1 cut(s) 354
AcsI RAATTY 1 cut(s) 250
AcvI CACGTG 1 cut(s) 104
AcyI GRCGYC 2 cut(s) 120, 141
AfiI CCNNNNNNNGG 2 cut(s) 34, 35
AflII CTTAAG 1 cut(s) 230
AflIII ACRYGT 3 cut(s) 101, 103, 376
AhdI GACNNNNNGTC 1 cut(s) 145
AleI CACNNNNGTG 1 cut(s) 60
AluBI AGCT 2 cut(s) 305, 325
AluI AGCT 2 cut(s) 305, 325
Alw21I GWGCWC 1 cut(s) 210
AlwNI CAGNNNCTG 1 cut(s) 363
ApeKI GCWGC 2 cut(s) 213, 345
ApoI RAATTY 1 cut(s) 250
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 223
AsuHPI GGTGA 1 cut(s) 359
AvaII GGWCC 1 cut(s) 223
BbrPI CACGTG 1 cut(s) 104
Bbv12I GWGCWC 1 cut(s) 210
BbvI GCAGC 2 cut(s) 200, 332
BfrI CTTAAG 1 cut(s) 230
BisI GCNGC 3 cut(s) 214, 346, 355
BlsI GCNGC 3 cut(s) 215, 347, 356
Bme18I GGWCC 1 cut(s) 223
BmeRI GACNNNNNGTC 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 223
BmiI GGNNCC 2 cut(s) 95, 245
BmsI GCATC 2 cut(s) 58, 357
BpmI CTGGAG 1 cut(s) 192
Bpu10I CCTNAGC 1 cut(s) 321
BsaAI YACGTR 2 cut(s) 104, 379
BsaHI GRCGYC 2 cut(s) 120, 141
BsaJI CCNNGG 1 cut(s) 87
BsaWI WCCGGW 1 cut(s) 369
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 35
Bse1I ACTGG 1 cut(s) 175
BseDI CCNNGG 1 cut(s) 87
BseLI CCNNNNNNNGG 2 cut(s) 34, 35
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 2 cut(s) 200, 332
BsiHKAI GWGCWC 1 cut(s) 210
BsiSI CCGG 1 cut(s) 370
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 2 cut(s) 34, 35
BsmFI GGGAC 1 cut(s) 128
BsmI GAATGC 1 cut(s) 290
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 1 cut(s) 197
BspACI CCGC 1 cut(s) 354
BspCNI CTCAG 1 cut(s) 313
BspLI GGNNCC 2 cut(s) 95, 245
BspTI CTTAAG 1 cut(s) 230
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 1 cut(s) 197
BssNAI GTATAC 1 cut(s) 6
BssNI GRCGYC 2 cut(s) 120, 141
BssT1I CCWWGG 1 cut(s) 87
Bst1107I GTATAC 1 cut(s) 6
Bst4CI ACNGT 2 cut(s) 116, 365
BstACI GRCGYC 2 cut(s) 120, 141
BstAFI CTTAAG 1 cut(s) 230
BstBAI YACGTR 2 cut(s) 104, 379
BstDEI CTNAG 2 cut(s) 255, 321
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 1 cut(s) 200
BstMBI GATC 1 cut(s) 197
BstMWI GCNNNNNNNGC 3 cut(s) 174, 351, 354
BstV1I GCAGC 2 cut(s) 200, 332
BstZ17I GTATAC 1 cut(s) 6
BtsI GCAGTG 1 cut(s) 208
BtsIMutI CAGTG 2 cut(s) 168, 208
CaiI CAGNNNCTG 1 cut(s) 363
CfoI GCGC 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 223
CviJI RGCY 6 cut(s) 11, 163, 305, 319, 325, 338
CviKI_1 RGCY 6 cut(s) 11, 163, 305, 319, 325, 338
DdeI CTNAG 2 cut(s) 255, 321
DpnI GATC 1 cut(s) 199
DpnII GATC 1 cut(s) 197
DriI GACNNNNNGTC 1 cut(s) 145
Eam1105I GACNNNNNGTC 1 cut(s) 145
Eco130I CCWWGG 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 223
Eco72I CACGTG 1 cut(s) 104
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaiI YATR 2 cut(s) 6, 166
FaqI GGGAC 1 cut(s) 128
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 214, 346, 355
Fsp4HI GCNGC 3 cut(s) 214, 346, 355
GlaI GCGC 1 cut(s) 72
GluI GCNGC 3 cut(s) 214, 346, 355
GsuI CTGGAG 1 cut(s) 192
HapII CCGG 1 cut(s) 370
HhaI GCGC 1 cut(s) 73
Hin1I GRCGYC 2 cut(s) 120, 141
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HpaII CCGG 1 cut(s) 370
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 1 cut(s) 81
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 1 cut(s) 236
HpyCH4III ACNGT 2 cut(s) 116, 365
HpyCH4IV ACGT 6 cut(s) 61, 103, 120, 132, 141, 378
HpyCH4V TGCA 3 cut(s) 168, 288, 348
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 351, 354
HpyF3I CTNAG 2 cut(s) 255, 321
HpySE526I ACGT 6 cut(s) 61, 103, 120, 132, 141, 378
Hsp92I GRCGYC 2 cut(s) 120, 141
HspAI GCGC 1 cut(s) 71
Kzo9I GATC 1 cut(s) 197
LmnI GCTCC 1 cut(s) 302
LpnPI CCDG 3 cut(s) 156, 333, 383
Lsp1109I GCAGC 2 cut(s) 200, 332
LweI GCATC 2 cut(s) 58, 357
MaeII ACGT 6 cut(s) 61, 103, 120, 132, 141, 378
MaeIII GTNAC 4 cut(s) 46, 116, 121, 365
MalI GATC 1 cut(s) 199
MboI GATC 1 cut(s) 197
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 2 cut(s) 250, 267
MnlI CCTC 2 cut(s) 28, 167
MseI TTAA 2 cut(s) 231, 270
MslI CAYNNNNRTG 1 cut(s) 60
MspCI CTTAAG 1 cut(s) 230
MspI CCGG 1 cut(s) 370
Mva1269I GAATGC 1 cut(s) 290
MwoI GCNNNNNNNGC 3 cut(s) 174, 351, 354
NdeII GATC 1 cut(s) 197
NlaIV GGNNCC 2 cut(s) 95, 245
NmuCI GTSAC 3 cut(s) 116, 121, 365
OliI CACNNNNGTG 1 cut(s) 60
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 1 cut(s) 290
PkrI GCNGC 3 cut(s) 215, 347, 356
PmaCI CACGTG 1 cut(s) 104
PmlI CACGTG 1 cut(s) 104
Ppu21I YACGTR 2 cut(s) 104, 379
PspCI CACGTG 1 cut(s) 104
PspN4I GGNNCC 2 cut(s) 95, 245
PspPI GGNCC 1 cut(s) 223
PstNI CAGNNNCTG 1 cut(s) 363
RseI CAYNNNNRTG 1 cut(s) 60
SaqAI TTAA 2 cut(s) 231, 270
SatI GCNGC 3 cut(s) 214, 346, 355
Sau3AI GATC 1 cut(s) 197
Sau96I GGNCC 1 cut(s) 223
SduI GDGCHC 1 cut(s) 210
SetI ASST 8 cut(s) 64, 106, 123, 135, 144, 307, 327, 381
SfaNI GCATC 2 cut(s) 58, 357
SinI GGWCC 1 cut(s) 223
SmiMI CAYNNNNRTG 1 cut(s) 60
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 2 cut(s) 250, 267
SsiI CCGC 1 cut(s) 354
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 2 cut(s) 116, 365
TaiI ACGT 6 cut(s) 64, 106, 123, 135, 144, 381
TasI AATT 2 cut(s) 250, 267
TauI GCSGC 1 cut(s) 357
Tru1I TTAA 2 cut(s) 231, 270
Tru9I TTAA 2 cut(s) 231, 270
TscAI CASTG 2 cut(s) 175, 215
TseFI GTSAC 3 cut(s) 116, 121, 365
TseI GCWGC 2 cut(s) 213, 345
Tsp45I GTSAC 3 cut(s) 116, 121, 365
TspRI CASTG 2 cut(s) 175, 215
Vha464I CTTAAG 1 cut(s) 230
VpaK11BI GGWCC 1 cut(s) 223
XapI RAATTY 1 cut(s) 250
XmiI GTMKAC 1 cut(s) 5
ZraI GACGTC 2 cut(s) 121, 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.