RLG00000016857

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
N/A
Physical Location & Seq
Reverse (-)
10695585 .. 10697289
1705 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016857

Sequence Viewer

Length: 756 bp
ATGGCGTCCAGCTACAAACTGAGAGCCTCCACGTCTGCTTCATCATCATCATCATCTGCTCGGTGGAAATACGACGTGTTCTTGAGTTTCAGGGGTGAAGATATCCGCAAGAAGTTCACGGACCATTTATACGACAAATTGCAGTGGCGGGGAATCAGAACTTTCAGAGACGACCCAAATCTTCAAAGAGGTACAAGTATTTCTCCAGAGCTCCTGGCTGCAATTGAACAATCAAACTTCGCAGTGGTATGCAACAGAGCTTATAAAAGAGATTGCGGAAGCACTGTGCCTACATTTGCATCATCAGAGTCCCAAGAGAATTTCGTCGGAATTGACGCCAAACTGATGGAAATAGATTATCTGTTGCATACAAAAGCAAGTGATGTTTGCTTTCTAGGAATATGGGACTTACATGGTATGGAGAGACAATATAAGAGCAAGGGATTAAACGAGCATGAAGCTCTTCAGCTCTTTAGTTGGAAAGCATTTAAGAAAGATAAACCTGAAGAAGGTTACAGGGAACTGTCTCAGAGTATCTTGGATTATGCAAAGGGACTTCCGTTAGCTCTTAATATTTTGGGATCTTTTCTGTGTAAGAGAGATCGGGGTGGTTGGGAAAGTGCAGTAGCTAAGCTAAAGAAAGCTCCTATTGATCAAAAACTCTTTGAAGCACTTAGAATAAGCTACGACGGGTTAGATGAGATGAGCCAACAAGTTTTACATGTTTCCTCAAGGGGCATGACAAAGAACGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

252

Amino Acids

28.55

Weight (kDa)

8.75

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 83 1.4e-15 TIR domain
TIR_2 PF13676 26 - 89 1.1e-06 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 264
AciI CCGC 3 cut(s) 106, 148, 276
AclWI GGATC 1 cut(s) 589
AcsI RAATTY 1 cut(s) 319
AcuI CTGAAG 2 cut(s) 449, 525
AcyI GRCGYC 2 cut(s) 5, 336
AfaI GTAC 1 cut(s) 193
AfiI CCNNNNNNNGG 1 cut(s) 509
AflIII ACRYGT 2 cut(s) 75, 721
AgsI TTSAA 3 cut(s) 185, 227, 668
AjiI CACGTC 2 cut(s) 33, 76
AjnI CCWGG 1 cut(s) 213
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 3 cut(s) 162, 418, 531
AlwI GGATC 1 cut(s) 589
ApeKI GCWGC 1 cut(s) 218
ApoI RAATTY 1 cut(s) 319
Asp700I GAANNNNTTC 1 cut(s) 462
AspS9I GGNCC 1 cut(s) 121
AsuHPI GGTGA 1 cut(s) 107
AvaII GGWCC 1 cut(s) 121
BanII GRGCYC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 205
BccI CCATC 1 cut(s) 340
BciT130I CCWGG 1 cut(s) 215
BclI TGATCA 1 cut(s) 652
BcoDI GTCTC 3 cut(s) 162, 418, 531
BfaI CTAG 1 cut(s) 395
BisI GCNGC 1 cut(s) 219
BlpI GCTNAGC 1 cut(s) 630
BlsI GCNGC 1 cut(s) 220
Bme1390I CCNGG 1 cut(s) 215
Bme18I GGWCC 1 cut(s) 121
BmgBI CACGTC 2 cut(s) 33, 76
BmgT120I GGNCC 1 cut(s) 121
BmrFI CCNGG 1 cut(s) 215
BmsI GCATC 1 cut(s) 308
BpmI CTGGAG 1 cut(s) 189
Bpu1102I GCTNAGC 1 cut(s) 630
BpuEI CTTGAG 2 cut(s) 103, 715
BsaHI GRCGYC 2 cut(s) 5, 336
Bsc4I CCNNNNNNNGG 1 cut(s) 509
BseBI CCWGG 1 cut(s) 215
BseLI CCNNNNNNNGG 1 cut(s) 509
BseMII CTCAG 2 cut(s) 11, 542
BseXI GCAGC 1 cut(s) 205
BsgI GTGCAG 1 cut(s) 642
BsiHKAI GWGCWC 1 cut(s) 213
BslFI GGGAC 3 cut(s) 295, 419, 567
BslI CCNNNNNNNGG 1 cut(s) 509
BsmAI GTCTC 3 cut(s) 162, 418, 531
BsmBI CGTCTC 1 cut(s) 162
BsmFI GGGAC 3 cut(s) 295, 419, 567
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 3 cut(s) 581, 601, 652
Bsp1720I GCTNAGC 1 cut(s) 630
BspACI CCGC 3 cut(s) 106, 148, 276
BspCNI CTCAG 2 cut(s) 12, 541
BspPI GGATC 1 cut(s) 589
BspQI GCTCTTC 1 cut(s) 468
BssMI GATC 3 cut(s) 581, 601, 652
BssNI GRCGYC 2 cut(s) 5, 336
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 2 cut(s) 286, 525
Bst6I CTCTTC 1 cut(s) 468
BstACI GRCGYC 2 cut(s) 5, 336
BstDEI CTNAG 4 cut(s) 20, 528, 630, 674
BstENI CCTNNNNNAGG 1 cut(s) 507
BstKTI GATC 3 cut(s) 584, 604, 655
BstMAI GTCTC 3 cut(s) 162, 418, 531
BstMBI GATC 3 cut(s) 581, 601, 652
BstNI CCWGG 1 cut(s) 215
BstNSI RCATGY 1 cut(s) 725
BstSCI CCNGG 1 cut(s) 213
BstV1I GCAGC 1 cut(s) 205
BstX2I RGATCY 1 cut(s) 581
BstXI CCANNNNNNTGG 1 cut(s) 346
BstYI RGATCY 1 cut(s) 581
BtrI CACGTC 2 cut(s) 33, 76
BtsI GCAGTG 2 cut(s) 149, 249
BtsIMutI CAGTG 3 cut(s) 149, 249, 282
Cfr13I GGNCC 1 cut(s) 121
CseI GACGC 1 cut(s) 344
Csp6I GTAC 1 cut(s) 192
CviAII CATG 4 cut(s) 413, 455, 722, 739
CviQI GTAC 1 cut(s) 192
DdeI CTNAG 4 cut(s) 20, 528, 630, 674
DpnI GATC 3 cut(s) 583, 603, 654
DpnII GATC 3 cut(s) 581, 601, 652
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
Ecl136II GAGCTC 1 cut(s) 211
Eco24I GRGCYC 1 cut(s) 213
Eco32I GATATC 1 cut(s) 103
Eco47I GGWCC 1 cut(s) 121
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 449, 525
EcoICRI GAGCTC 1 cut(s) 211
EcoNI CCTNNNNNAGG 1 cut(s) 507
EcoRII CCWGG 1 cut(s) 213
EcoRV GATATC 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 213
Esp3I CGTCTC 1 cut(s) 162
FaeI CATG 4 cut(s) 416, 458, 725, 742
FaqI GGGAC 3 cut(s) 295, 419, 567
FatI CATG 4 cut(s) 412, 454, 721, 738
FauI CCCGC 1 cut(s) 141
FbaI TGATCA 1 cut(s) 652
Fnu4HI GCNGC 1 cut(s) 219
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 1 cut(s) 219
FspBI CTAG 1 cut(s) 395
GluI GCNGC 1 cut(s) 219
GsuI CTGGAG 1 cut(s) 189
HgaI GACGC 1 cut(s) 344
Hin1I GRCGYC 2 cut(s) 5, 336
Hin1II CATG 4 cut(s) 416, 458, 725, 742
HinfI GANTC 2 cut(s) 153, 308
HphI GGTGA 1 cut(s) 107
Hpy166II GTNNAC 1 cut(s) 117
Hpy188I TCNGA 5 cut(s) 158, 167, 307, 329, 531
Hpy188III TCNNGA 2 cut(s) 82, 206
Hpy8I GTNNAC 1 cut(s) 117
Hpy99I CGWCG 3 cut(s) 77, 329, 692
HpyAV CCTTC 1 cut(s) 503
HpyCH4III ACNGT 2 cut(s) 286, 525
HpyCH4IV ACGT 2 cut(s) 32, 75
HpyCH4V TGCA 7 cut(s) 142, 221, 252, 299, 367, 548, 623
HpyF3I CTNAG 4 cut(s) 20, 528, 630, 674
HpySE526I ACGT 2 cut(s) 32, 75
Hsp92I GRCGYC 2 cut(s) 5, 336
Hsp92II CATG 4 cut(s) 416, 458, 725, 742
Ksp22I TGATCA 1 cut(s) 652
Kzo9I GATC 3 cut(s) 581, 601, 652
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 2 cut(s) 216, 649
LpnPI CCDG 7 cut(s) 22, 76, 200, 219, 227, 502, 516
Lsp1109I GCAGC 1 cut(s) 205
LweI GCATC 1 cut(s) 308
MaeI CTAG 1 cut(s) 395
MaeII ACGT 2 cut(s) 32, 75
MaeIII GTNAC 1 cut(s) 512
MalI GATC 3 cut(s) 583, 603, 654
MboI GATC 3 cut(s) 581, 601, 652
MboII GAAGA 4 cut(s) 110, 173, 455, 518
MfeI CAATTG 1 cut(s) 222
MflI RGATCY 1 cut(s) 581
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 4 cut(s) 137, 222, 319, 330
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 2 cut(s) 307, 458
MnlI CCTC 3 cut(s) 37, 182, 739
MroXI GAANNNNTTC 1 cut(s) 462
MseI TTAA 3 cut(s) 446, 489, 570
MspR9I CCNGG 1 cut(s) 215
MunI CAATTG 1 cut(s) 222
MvaI CCWGG 1 cut(s) 215
NdeII GATC 3 cut(s) 581, 601, 652
NlaIII CATG 4 cut(s) 416, 458, 725, 742
NspI RCATGY 1 cut(s) 725
PciI ACATGT 1 cut(s) 721
PciSI GCTCTTC 1 cut(s) 468
PdmI GAANNNNTTC 1 cut(s) 462
PfeI GAWTC 1 cut(s) 153
PkrI GCNGC 1 cut(s) 220
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PscI ACATGT 1 cut(s) 721
PsiI TTATAA 1 cut(s) 264
Psp124BI GAGCTC 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspPI GGNCC 1 cut(s) 121
PsuI RGATCY 1 cut(s) 581
RsaI GTAC 1 cut(s) 193
RsaNI GTAC 1 cut(s) 192
SacI GAGCTC 1 cut(s) 213
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 3 cut(s) 446, 489, 570
SatI GCNGC 1 cut(s) 219
Sau3AI GATC 3 cut(s) 581, 601, 652
Sau96I GGNCC 1 cut(s) 121
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 215
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 1 cut(s) 308
SinI GGWCC 1 cut(s) 121
SmlI CTYRAG 2 cut(s) 82, 730
SmoI CTYRAG 2 cut(s) 82, 730
Sse9I AATT 4 cut(s) 137, 222, 319, 330
SsiI CCGC 3 cut(s) 106, 148, 276
SspI AATATT 1 cut(s) 574
SspMI CTAG 1 cut(s) 395
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 1 cut(s) 213
TaaI ACNGT 2 cut(s) 286, 525
TaiI ACGT 2 cut(s) 35, 78
TasI AATT 4 cut(s) 137, 222, 319, 330
TfiI GAWTC 1 cut(s) 153
Tru1I TTAA 3 cut(s) 446, 489, 570
Tru9I TTAA 3 cut(s) 446, 489, 570
TscAI CASTG 3 cut(s) 149, 249, 289
TseI GCWGC 1 cut(s) 218
TspDTI ATGAA 2 cut(s) 30, 471
TspGWI ACGGA 2 cut(s) 134, 549
TspRI CASTG 3 cut(s) 149, 249, 289
VpaK11BI GGWCC 1 cut(s) 121
XagI CCTNNNNNAGG 1 cut(s) 507
XapI RAATTY 1 cut(s) 319
XceI RCATGY 1 cut(s) 725
XmnI GAANNNNTTC 1 cut(s) 462
XspI CTAG 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.