RLG00000021951

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
N/A
Physical Location & Seq
Reverse (-)
79488148 .. 79488801
654 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021951

Sequence Viewer

Length: 654 bp
ATGGTCATTAATGATTGCCACAAATTGGAGGAATTGCCTGCAGAGCTCTGCGAGCTGATTCATCTGAAAAAGCTCACAATCACCGCTTGTTCGAAGTTATCAGTCTTGCCTGAAGGGATTGGAGATCTGGTCAATTTAGAAGCGTTGAGGCTTCGGTTCAATTTCGAGCTTTCAGAGATTCCAGACTCGATTGAAAATCTCAACAAATTAAAATTTCTTGATATTACTGGTTGCCGGAAAATTGAGAAATTGCCTGAACACATGGGTGAGCTCAAGTGTTTGACAAATCTCAGCATCGTGTGCTTGCCGGAATCGATTGGAAATTTGGTCAATTTAGAAGTATTGAGTCTAAGGCTCTGTTACAGTTTGTCAGAGTTGCCGGAGTCGATTAGAAATCTCAAAAAGTTGACCTTTCTTGAGATCCATTGTTGCTACGATATCAAGGAGCTGCCTGAACAGATTGGTGAATTGAAGAGTTTAAGAAAGCTGGAAATGAGTCTTTGCGATGAATTGAGAGAACTGCCTCAATCAGTTTCAGATCTTGAGCAGTTGGAGGAAGTGATATGCGACGATTGGCACGCAACACATTTATGGGCACCCTTGTTACCCACTCTCAAAAACCTTCGCATCGTGGTGTGGAAGGGCGGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

218

Amino Acids

24.83

Weight (kDa)

5.08

Isoelectric Point (pI)

51.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 4 - 101 1.6e-13 Leucine-rich repeat region
LRR_8 PF13855 21 - 79 3.4e-06 Leucine rich repeat
LRR_14 PF23598 101 - 208 1e-12 Leucine-rich repeat region
LRR_13 PF23286 110 - 180 2.1e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 595
AccB7I CCANNNNNTGG 1 cut(s) 25
AciI CCGC 2 cut(s) 84, 645
AclWI GGATC 1 cut(s) 415
AcsI RAATTY 2 cut(s) 212, 322
AcuI CTGAAG 1 cut(s) 132
AfiI CCNNNNNNNGG 1 cut(s) 25
AgsI TTSAA 3 cut(s) 160, 194, 472
AleI CACNNNNGTG 1 cut(s) 264
AluBI AGCT 7 cut(s) 46, 55, 73, 169, 271, 448, 487
AluI AGCT 7 cut(s) 46, 55, 73, 169, 271, 448, 487
Alw21I GWGCWC 2 cut(s) 48, 273
AlwI GGATC 1 cut(s) 415
ApeKI GCWGC 1 cut(s) 448
ApoI RAATTY 2 cut(s) 212, 322
AseI ATTAAT 1 cut(s) 9
AsuHPI GGTGA 3 cut(s) 73, 278, 476
AsuII TTCGAA 1 cut(s) 92
BaeGI GKGCMC 1 cut(s) 598
BanI GGYRCC 1 cut(s) 595
BanII GRGCYC 2 cut(s) 48, 273
BarI GAAGNNNNNNTAC 1 cut(s) 632
Bbv12I GWGCWC 2 cut(s) 48, 273
BbvI GCAGC 1 cut(s) 435
BciVI GTATCC 1 cut(s) 641
BfmI CTRYAG 1 cut(s) 39
BfuI GTATCC 1 cut(s) 641
BglII AGATCT 2 cut(s) 124, 538
BisI GCNGC 1 cut(s) 449
BlsI GCNGC 1 cut(s) 450
BmiI GGNNCC 1 cut(s) 597
BmsI GCATC 2 cut(s) 303, 636
Bpu14I TTCGAA 1 cut(s) 92
BpuEI CTTGAG 3 cut(s) 257, 437, 563
Bsa29I ATCGAT 1 cut(s) 314
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bsc4I CCNNNNNNNGG 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 232
BseCI ATCGAT 1 cut(s) 314
BseLI CCNNNNNNNGG 1 cut(s) 25
BseMII CTCAG 1 cut(s) 304
BseNI ACTGG 1 cut(s) 232
BseSI GKGCMC 1 cut(s) 598
BseXI GCAGC 1 cut(s) 435
BshNI GGYRCC 1 cut(s) 595
BshVI ATCGAT 1 cut(s) 314
BsiHKAI GWGCWC 2 cut(s) 48, 273
BsiSI CCGG 3 cut(s) 235, 308, 380
BslI CCNNNNNNNGG 1 cut(s) 25
Bsp119I TTCGAA 1 cut(s) 92
Bsp1286I GDGCHC 3 cut(s) 48, 273, 598
Bsp143I GATC 3 cut(s) 124, 420, 538
BspACI CCGC 2 cut(s) 84, 645
BspCNI CTCAG 1 cut(s) 303
BspDI ATCGAT 1 cut(s) 314
BspLI GGNNCC 1 cut(s) 597
BspMAI CTGCAG 1 cut(s) 43
BspPI GGATC 1 cut(s) 415
BspT104I TTCGAA 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 595
BsrI ACTGG 1 cut(s) 232
BssMI GATC 3 cut(s) 124, 420, 538
Bst4CI ACNGT 1 cut(s) 365
Bst6I CTCTTC 1 cut(s) 467
BstAPI GCANNNNNTGC 1 cut(s) 300
BstBI TTCGAA 1 cut(s) 92
BstC8I GCNNGC 4 cut(s) 39, 53, 305, 579
BstDEI CTNAG 2 cut(s) 290, 350
BstKTI GATC 3 cut(s) 127, 423, 541
BstMBI GATC 3 cut(s) 124, 420, 538
BstMWI GCNNNNNNNGC 3 cut(s) 43, 52, 300
BstSFI CTRYAG 1 cut(s) 39
BstSLI GKGCMC 1 cut(s) 598
BstV1I GCAGC 1 cut(s) 435
BstX2I RGATCY 3 cut(s) 124, 420, 538
BstYI RGATCY 3 cut(s) 124, 420, 538
Bsu15I ATCGAT 1 cut(s) 314
BsuI GTATCC 1 cut(s) 641
BsuTUI ATCGAT 1 cut(s) 314
BtgZI GCGATG 1 cut(s) 519
Cac8I GCNNGC 4 cut(s) 39, 53, 305, 579
ClaI ATCGAT 1 cut(s) 314
CviAII CATG 1 cut(s) 262
CviJI RGCY 9 cut(s) 46, 55, 73, 151, 169, 271, 355, 448, 487
CviKI_1 RGCY 9 cut(s) 46, 55, 73, 151, 169, 271, 355, 448, 487
DdeI CTNAG 2 cut(s) 290, 350
DpnI GATC 3 cut(s) 126, 422, 540
DpnII GATC 3 cut(s) 124, 420, 538
Eam1104I CTCTTC 1 cut(s) 467
EarI CTCTTC 1 cut(s) 467
Ecl136II GAGCTC 2 cut(s) 46, 271
Eco24I GRGCYC 2 cut(s) 48, 273
Eco32I GATATC 1 cut(s) 439
Eco53kI GAGCTC 2 cut(s) 46, 271
Eco57I CTGAAG 1 cut(s) 132
EcoICRI GAGCTC 2 cut(s) 46, 271
EcoRV GATATC 1 cut(s) 439
EcoT38I GRGCYC 2 cut(s) 48, 273
FaeI CATG 1 cut(s) 265
FaiI YATR 3 cut(s) 263, 565, 592
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FatI CATG 1 cut(s) 261
Fnu4HI GCNGC 1 cut(s) 449
FriOI GRGCYC 2 cut(s) 48, 273
Fsp4HI GCNGC 1 cut(s) 449
GluI GCNGC 1 cut(s) 449
HapII CCGG 3 cut(s) 235, 308, 380
Hin1II CATG 1 cut(s) 265
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 7 cut(s) 58, 178, 185, 311, 346, 383, 496
HpaII CCGG 3 cut(s) 235, 308, 380
HphI GGTGA 3 cut(s) 73, 278, 476
Hpy166II GTNNAC 1 cut(s) 408
Hpy188I TCNGA 4 cut(s) 66, 175, 373, 538
Hpy188III TCNNGA 4 cut(s) 182, 218, 416, 542
Hpy8I GTNNAC 1 cut(s) 408
Hpy99I CGWCG 1 cut(s) 572
HpyAV CCTTC 3 cut(s) 107, 632, 634
HpyCH4III ACNGT 1 cut(s) 365
HpyCH4V TGCA 1 cut(s) 41
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 52, 300
HpyF3I CTNAG 2 cut(s) 290, 350
Hsp92II CATG 1 cut(s) 265
Kzo9I GATC 3 cut(s) 124, 420, 538
LmnI GCTCC 1 cut(s) 445
Lsp1109I GCAGC 1 cut(s) 435
LweI GCATC 2 cut(s) 303, 636
MaeIII GTNAC 2 cut(s) 359, 603
MalI GATC 3 cut(s) 126, 422, 540
MboI GATC 3 cut(s) 124, 420, 538
MboII GAAGA 1 cut(s) 484
MflI RGATCY 3 cut(s) 124, 420, 538
MhlI GDGCHC 3 cut(s) 48, 273, 598
MlyI GAGTC 4 cut(s) 179, 355, 392, 505
MmeI TCCRAC 1 cut(s) 531
MnlI CCTC 4 cut(s) 22, 141, 534, 547
MseI TTAA 3 cut(s) 9, 209, 479
MslI CAYNNNNRTG 3 cut(s) 264, 589, 632
MspI CCGG 3 cut(s) 235, 308, 380
MwoI GCNNNNNNNGC 3 cut(s) 43, 52, 300
NdeII GATC 3 cut(s) 124, 420, 538
NlaIII CATG 1 cut(s) 265
NlaIV GGNNCC 1 cut(s) 597
NspV TTCGAA 1 cut(s) 92
OliI CACNNNNGTG 1 cut(s) 264
PfeI GAWTC 3 cut(s) 58, 178, 311
PflMI CCANNNNNTGG 1 cut(s) 25
PkrI GCNGC 1 cut(s) 450
PleI GAGTC 4 cut(s) 179, 354, 391, 504
PpsI GAGTC 4 cut(s) 179, 354, 391, 504
PshBI ATTAAT 1 cut(s) 9
Psp124BI GAGCTC 2 cut(s) 48, 273
PspN4I GGNNCC 1 cut(s) 597
PstI CTGCAG 1 cut(s) 43
PsuI RGATCY 3 cut(s) 124, 420, 538
RseI CAYNNNNRTG 3 cut(s) 264, 589, 632
SacI GAGCTC 2 cut(s) 48, 273
SaqAI TTAA 3 cut(s) 9, 209, 479
SatI GCNGC 1 cut(s) 449
Sau3AI GATC 3 cut(s) 124, 420, 538
SchI GAGTC 4 cut(s) 179, 355, 392, 505
SduI GDGCHC 3 cut(s) 48, 273, 598
SetI ASST 9 cut(s) 48, 57, 75, 171, 273, 413, 450, 489, 624
SfaNI GCATC 2 cut(s) 303, 636
SfcI CTRYAG 1 cut(s) 39
SfuI TTCGAA 1 cut(s) 92
SmiMI CAYNNNNRTG 3 cut(s) 264, 589, 632
SmlI CTYRAG 3 cut(s) 272, 416, 542
SmoI CTYRAG 3 cut(s) 272, 416, 542
SsiI CCGC 2 cut(s) 84, 645
SstI GAGCTC 2 cut(s) 48, 273
TaaI ACNGT 1 cut(s) 365
TaqI TCGA 5 cut(s) 92, 165, 188, 314, 386
TfiI GAWTC 3 cut(s) 58, 178, 311
Tru1I TTAA 3 cut(s) 9, 209, 479
Tru9I TTAA 3 cut(s) 9, 209, 479
TseI GCWGC 1 cut(s) 448
TspDTI ATGAA 2 cut(s) 50, 522
Van91I CCANNNNNTGG 1 cut(s) 25
VspI ATTAAT 1 cut(s) 9
XapI RAATTY 2 cut(s) 212, 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.