RLG00000024482
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
N/A
Physical Location & Seq
Reverse (-)
36303870 .. 36304893
1024 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024482

Sequence Viewer

Length: 774 bp
ATGGTTTTGGCAAAGATGCGGGAGATTGCTGAGGAGTTCCTTGGCTCAACAGTGAAAAATGCTGTTATCACAGTGCCCGCTTACTTCAGTGACTCACAACGCCAGGCGACAAGAAATGCAGGTGAATTTGCAGGTCTAAAGGTGATGCGTATTATCAATGAACCAACAGCTGCAGCAATTGCTTATGGCCTTGAAAAAAAGGCCGGTTGGTATAGCAAGAGATATGTGATGATCTTTGATTTGGGTGGTGGAACTTTAGATGTCTCACTGCTTACGATTAGTTCTGGTGTCTTCGAAGTGAAAGCCACGGCTGGAGACACTCACCTTGGTGGTGAAGATTTTGATAACAGAATGGTAGATTTCTGTGCTGCATTGATTTGCTGCAATCTTGAGTACGTGGGAATGGAAATGGGAAGCTTCAAGACTTCAATCTCTTGGATGTCACACCTATGTCACTTGGAGTGCGGATTAAAAAAAGAGAATATCATGCATTTTGTTGTTCAAAGAAACACCAGAATTCCGGTCAAGAAGAATACAACAATATGTACCCGTTATGACAACCGAAGTGTTGTTGCCATCCCAATTTTTGAGGGTGAGAATAAAAGCACTTTGAACAATAACCTTTTGGGTACATTTAGCCTCGTGGACATTCCTCCAGATGCTAAGGGTTATTCATTCGATGTTTGCTTTGAAATTGATGAAAATGGAATCCTAAGTGTCTCCGCTGAGGACAAGTCCACTGGCCAGAAGAAAGTGACAAGAAGAAATTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

258

Amino Acids

28.39

Weight (kDa)

6.44

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 1 - 124 4.8e-60 Hsp70 protein
MreB_Mbl PF06723 18 - 121 4.3e-09 MreB/Mbl protein
HSP70 PF00012 159 - 251 6.3e-19 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 110
Acc36I ACCTGC 2 cut(s) 110, 122
AciI CCGC 4 cut(s) 19, 78, 465, 723
AcoI YGGCCR 1 cut(s) 742
AcsI RAATTY 2 cut(s) 125, 516
AcuI CTGAAG 1 cut(s) 70
AfaI GTAC 3 cut(s) 395, 547, 631
AgsI TTSAA 6 cut(s) 194, 421, 429, 503, 613, 692
AjnI CCWGG 1 cut(s) 102
AleI CACNNNNGTG 1 cut(s) 327
AluBI AGCT 2 cut(s) 170, 417
AluI AGCT 2 cut(s) 170, 417
Alw26I GTCTC 3 cut(s) 268, 309, 724
AoxI GGCC 3 cut(s) 187, 201, 742
ApeKI GCWGC 4 cut(s) 170, 173, 368, 381
ApoI RAATTY 2 cut(s) 125, 516
AsuHPI GGTGA 5 cut(s) 134, 154, 314, 344, 605
AsuII TTCGAA 1 cut(s) 294
BaeGI GKGCMC 1 cut(s) 78
BalI TGGCCA 1 cut(s) 744
BauI CACGAG 1 cut(s) 641
BbsI GAAGAC 1 cut(s) 283
BbvCI CCTCAGC 2 cut(s) 30, 726
BbvI GCAGC 4 cut(s) 157, 185, 355, 368
BccI CCATC 1 cut(s) 584
BceAI ACGGC 1 cut(s) 324
BciT130I CCWGG 1 cut(s) 104
BcoDI GTCTC 3 cut(s) 268, 309, 724
BfmI CTRYAG 1 cut(s) 171
BfuAI ACCTGC 2 cut(s) 110, 122
BisI GCNGC 4 cut(s) 171, 174, 369, 382
BlsI GCNGC 4 cut(s) 172, 175, 370, 383
Bme1390I CCNGG 1 cut(s) 104
BmrFI CCNGG 1 cut(s) 104
BmsI GCATC 3 cut(s) 6, 135, 649
BpiI GAAGAC 1 cut(s) 283
BpmI CTGGAG 2 cut(s) 333, 639
Bpu10I CCTNAGC 3 cut(s) 30, 663, 726
Bpu14I TTCGAA 1 cut(s) 294
BpuEI CTTGAG 1 cut(s) 410
BsaAI YACGTR 1 cut(s) 397
BsaJI CCNNGG 3 cut(s) 40, 306, 325
BsaWI WCCGGW 1 cut(s) 520
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 745
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 3 cut(s) 40, 306, 325
BseGI GGATG 2 cut(s) 444, 576
BseMII CTCAG 2 cut(s) 21, 717
BseNI ACTGG 1 cut(s) 745
BseRI GAGGAG 1 cut(s) 47
BseSI GKGCMC 1 cut(s) 78
BseXI GCAGC 4 cut(s) 157, 185, 355, 368
BshFI GGCC 3 cut(s) 189, 203, 744
BsiSI CCGG 2 cut(s) 204, 521
BsmAI GTCTC 3 cut(s) 268, 309, 724
BsnI GGCC 3 cut(s) 189, 203, 744
Bsp119I TTCGAA 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 1 cut(s) 231
BspACI CCGC 4 cut(s) 19, 78, 465, 723
BspANI GGCC 3 cut(s) 189, 203, 744
BspCNI CTCAG 2 cut(s) 22, 718
BspMAI CTGCAG 1 cut(s) 175
BspMI ACCTGC 2 cut(s) 110, 122
BspT104I TTCGAA 1 cut(s) 294
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 1 cut(s) 745
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 3 cut(s) 40, 306, 325
BssMI GATC 1 cut(s) 231
BssSI CACGAG 1 cut(s) 641
BssT1I CCWWGG 2 cut(s) 40, 325
Bst2BI CACGAG 1 cut(s) 641
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 2 cut(s) 52, 73
BstAPI GCANNNNNTGC 1 cut(s) 179
BstBAI YACGTR 1 cut(s) 397
BstBI TTCGAA 1 cut(s) 294
BstC8I GCNNGC 1 cut(s) 78
BstDEI CTNAG 4 cut(s) 30, 663, 713, 726
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 2 cut(s) 444, 576
BstKTI GATC 1 cut(s) 234
BstMAI GTCTC 3 cut(s) 268, 309, 724
BstMBI GATC 1 cut(s) 231
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 1 cut(s) 104
BstSCI CCNGG 1 cut(s) 102
BstSFI CTRYAG 1 cut(s) 171
BstSLI GKGCMC 1 cut(s) 78
BstV1I GCAGC 4 cut(s) 157, 185, 355, 368
BstV2I GAAGAC 1 cut(s) 283
BsuRI GGCC 3 cut(s) 189, 203, 744
BtgI CCRYGG 1 cut(s) 306
BtsCI GGATG 2 cut(s) 444, 576
BtsI GCAGTG 1 cut(s) 266
BtsIMutI CAGTG 5 cut(s) 57, 78, 94, 266, 738
BveI ACCTGC 2 cut(s) 110, 122
Cac8I GCNNGC 1 cut(s) 78
Cfr10I RCCGGY 1 cut(s) 203
Csp6I GTAC 3 cut(s) 394, 546, 630
CviAII CATG 1 cut(s) 487
CviJI RGCY 9 cut(s) 45, 170, 189, 203, 305, 311, 417, 639, 744
CviKI_1 RGCY 9 cut(s) 45, 170, 189, 203, 305, 311, 417, 639, 744
CviQI GTAC 3 cut(s) 394, 546, 630
DdeI CTNAG 4 cut(s) 30, 663, 713, 726
DpnI GATC 1 cut(s) 233
DpnII GATC 1 cut(s) 231
EaeI YGGCCR 1 cut(s) 742
Eco130I CCWWGG 2 cut(s) 40, 325
Eco57I CTGAAG 1 cut(s) 70
EcoRI GAATTC 1 cut(s) 516
EcoRII CCWGG 1 cut(s) 102
EcoT14I CCWWGG 2 cut(s) 40, 325
EcoT22I ATGCAT 1 cut(s) 492
ErhI CCWWGG 2 cut(s) 40, 325
FaeI CATG 1 cut(s) 490
FaiI YATR 7 cut(s) 186, 213, 225, 451, 488, 544, 555
FatI CATG 1 cut(s) 486
FauI CCCGC 2 cut(s) 12, 85
Fnu4HI GCNGC 4 cut(s) 171, 174, 369, 382
FokI GGATG 2 cut(s) 451, 563
Fsp4HI GCNGC 4 cut(s) 171, 174, 369, 382
GluI GCNGC 4 cut(s) 171, 174, 369, 382
GsuI CTGGAG 2 cut(s) 333, 639
HaeIII GGCC 3 cut(s) 189, 203, 744
HapII CCGG 2 cut(s) 204, 521
Hin1II CATG 1 cut(s) 490
HindIII AAGCTT 1 cut(s) 415
HinfI GANTC 2 cut(s) 92, 708
HpaII CCGG 2 cut(s) 204, 521
HphI GGTGA 5 cut(s) 134, 154, 314, 344, 605
Hpy166II GTNNAC 2 cut(s) 646, 738
Hpy188III TCNNGA 4 cut(s) 389, 421, 526, 656
Hpy8I GTNNAC 2 cut(s) 646, 738
HpyCH4III ACNGT 2 cut(s) 52, 73
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 6 cut(s) 119, 131, 173, 371, 384, 490
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 4 cut(s) 30, 663, 713, 726
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 1 cut(s) 490
Kzo9I GATC 1 cut(s) 231
Lsp1109I GCAGC 4 cut(s) 157, 185, 355, 368
LweI GCATC 3 cut(s) 6, 135, 649
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 4 cut(s) 89, 441, 452, 754
MalI GATC 1 cut(s) 233
MboI GATC 1 cut(s) 231
MboII GAAGA 5 cut(s) 283, 347, 541, 760, 774
MfeI CAATTG 1 cut(s) 177
MhlI GDGCHC 1 cut(s) 78
MlsI TGGCCA 1 cut(s) 744
MluCI AATT 6 cut(s) 125, 177, 516, 582, 693, 766
MluNI TGGCCA 1 cut(s) 744
MlyI GAGTC 1 cut(s) 86
MnlI CCTC 5 cut(s) 25, 583, 650, 663, 721
Mox20I TGGCCA 1 cut(s) 744
Mph1103I ATGCAT 1 cut(s) 492
MscI TGGCCA 1 cut(s) 744
MseI TTAA 1 cut(s) 470
MslI CAYNNNNRTG 2 cut(s) 327, 448
Msp20I TGGCCA 1 cut(s) 744
MspA1I CMGCKG 2 cut(s) 170, 725
MspI CCGG 2 cut(s) 204, 521
MspR9I CCNGG 1 cut(s) 104
MunI CAATTG 1 cut(s) 177
MvaI CCWGG 1 cut(s) 104
MwoI GCNNNNNNNGC 1 cut(s) 179
NdeII GATC 1 cut(s) 231
NlaIII CATG 1 cut(s) 490
NmuCI GTSAC 4 cut(s) 89, 441, 452, 754
NsiI ATGCAT 1 cut(s) 492
NspV TTCGAA 1 cut(s) 294
OliI CACNNNNGTG 1 cut(s) 327
PaqCI CACCTGC 1 cut(s) 110
PfeI GAWTC 1 cut(s) 708
PkrI GCNGC 4 cut(s) 172, 175, 370, 383
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
Ppu21I YACGTR 1 cut(s) 397
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PstI CTGCAG 1 cut(s) 175
PvuII CAGCTG 1 cut(s) 170
RsaI GTAC 3 cut(s) 395, 547, 631
RsaNI GTAC 3 cut(s) 394, 546, 630
RseI CAYNNNNRTG 2 cut(s) 327, 448
SaqAI TTAA 1 cut(s) 470
SatI GCNGC 4 cut(s) 171, 174, 369, 382
Sau3AI GATC 1 cut(s) 231
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 104
SduI GDGCHC 1 cut(s) 78
SetI ASST 9 cut(s) 124, 136, 144, 172, 327, 399, 419, 450, 624
SfaNI GCATC 3 cut(s) 6, 135, 649
SfcI CTRYAG 1 cut(s) 171
SfuI TTCGAA 1 cut(s) 294
SmiMI CAYNNNNRTG 2 cut(s) 327, 448
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 6 cut(s) 125, 177, 516, 582, 693, 766
SsiI CCGC 4 cut(s) 19, 78, 465, 723
StyD4I CCNGG 1 cut(s) 102
StyI CCWWGG 2 cut(s) 40, 325
TaaI ACNGT 2 cut(s) 52, 73
TaiI ACGT 1 cut(s) 399
TaqI TCGA 2 cut(s) 294, 678
TasI AATT 6 cut(s) 125, 177, 516, 582, 693, 766
TfiI GAWTC 1 cut(s) 708
Tru1I TTAA 1 cut(s) 470
Tru9I TTAA 1 cut(s) 470
TscAI CASTG 5 cut(s) 57, 78, 94, 273, 745
TseFI GTSAC 4 cut(s) 89, 441, 452, 754
TseI GCWGC 4 cut(s) 170, 173, 368, 381
Tsp45I GTSAC 4 cut(s) 89, 441, 452, 754
TspDTI ATGAA 3 cut(s) 174, 663, 714
TspRI CASTG 5 cut(s) 57, 78, 94, 273, 745
XapI RAATTY 2 cut(s) 125, 516
Zsp2I ATGCAT 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.