RLG00000035674

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
N/A
Physical Location & Seq
Reverse (-)
70084875 .. 70091381
6507 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035674

Sequence Viewer

Length: 1263 bp
ATGATGGAGGATGACGAGTACTGGGCTTGGGAGTCCTTCTATTCAGATTGGAGCACCTCCTACAGTGGGTCTCATGATTCAGCTCCTCAGTGGAAGTACGATGTCTTTTTGAGTTTTAGGGGCGAAGACACGAGAACCAGTTTCACTGACCATCTTTACGAAAAATTGGTGTGGCGAGGAATCAAAACTTTCAGGGATGACCCAGAAATTGAAAGGGGGTCAACTATCTCTGCAGAGCTCTCGGCAGCCATTAAAGATTCACGGTTCGCAATCATTGTTCTCTCCCCAAACTACGCTTCTTCCACTTGGTGCTTGGATGAACTTGAAATGATTAATCAGTCGATTGGAATCTCCAGACGTTTGCCAGTCTTCTATCATGTTGATCCTTCTGATGTTCGAAGACAAACTGGCCCTTTCGCCGAAGCCTTCACTAAACATGAACATGACTTCAAGATATACCCGAAGAAGGTGCGTTGGTGGAGACAGGCTTTGACAAGTGTGAGTAATGTTGCTGGATGGACTACTTCTAAGTATAGGTATGAAACAGAGCTTATCAAAGAAATTGTAGATGTAATAGGGGAGAAACACTTGGGTAACTTGAAGTATATTGACCTTAGTTTCTCCAAAAATTTGATCAAGACCCCAGATTTCACTGGTATTCCAAATCTTGAAAGGTTGGTTCTTGAAAGTTGCACTAATGTAATTGAGATTCACTCATCTATTGCATTTCTCAAAAAGCTTAAAATTTTGAATTTCACAAACTGTAAAAGTATCAGGAGTCTTCCAAGTGAGATAGAAATGGAATCTCTTGAAGTTTTTATTCTTTCGGGATGCTCAAAATTGAAAAGCATTCCAGAATTTTTGGGACCAATGAAAAATTTATCAATGCTTTCTTTGGATGGGACAGCTATTGAGGAATCACCTCCATCAATTGAATGTTTGATTCGCCTTATTTCACTAGATCTAAGAGATTGCAAAAGTCTATTGTGTCTTCCGAGTGTCCTCTGTAGCATGAAATCTCTTAGAAGTCTCAATATGTCTGGATGCTCAAAACTGCGCAAATTCCCTGATAGTGTAGGGCAGATTGAATGCTTGAAAGAGTTAGATCTCAGTGGAACTGCATTAAGCGAGCTTCCATCCTCCATTGTTCTGATGAAAAATCTGAAGCTACACCTTACTTATGGCGTGGAGGGCCATGATAGGGTCATGAAAGCAACTAATGCTGCTGTAGACCTCGGATACAATCCTGTTAAAAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

421

Amino Acids

47.75

Weight (kDa)

6.06

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 32 - 196 7.1e-48 TIR domain
TIR_2 PF13676 35 - 129 1.2e-14 TIR domain
LRR_13 PF23286 268 - 354 2.4e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 317 - 389 4.9e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1058
AccI GTMKAC 1 cut(s) 1230
AclWI GGATC 1 cut(s) 377
AcsI RAATTY 6 cut(s) 628, 744, 751, 857, 877, 1061
AcuI CTGAAG 1 cut(s) 1184
AdeI CACNNNGTG 1 cut(s) 309
AfaI GTAC 2 cut(s) 20, 98
AfiI CCNNNNNNNGG 3 cut(s) 66, 466, 1201
AjuI GAANNNNNNNTTGG 2 cut(s) 280, 312
AluBI AGCT 7 cut(s) 83, 238, 550, 739, 908, 1132, 1168
AluI AGCT 7 cut(s) 83, 238, 550, 739, 908, 1132, 1168
Alw21I GWGCWC 2 cut(s) 56, 240
Alw26I GTCTC 3 cut(s) 75, 475, 1034
AlwI GGATC 1 cut(s) 377
AoxI GGCC 2 cut(s) 409, 1192
ApeKI GCWGC 2 cut(s) 245, 1223
ApoI RAATTY 6 cut(s) 628, 744, 751, 857, 877, 1061
AseI ATTAAT 1 cut(s) 333
AspLEI GCGC 1 cut(s) 1059
AspS9I GGNCC 3 cut(s) 410, 866, 1192
AsuHPI GGTGA 1 cut(s) 912
AsuII TTCGAA 1 cut(s) 397
AvaII GGWCC 1 cut(s) 866
BanII GRGCYC 1 cut(s) 240
BauI CACGAG 1 cut(s) 130
BbsI GAAGAC 5 cut(s) 132, 361, 406, 773, 983
Bbv12I GWGCWC 2 cut(s) 56, 240
BbvI GCAGC 2 cut(s) 257, 1210
BccI CCATC 5 cut(s) 159, 510, 893, 934, 1144
BcgI CGANNNNNNTGC 4 cut(s) 222, 256, 451, 485
BciVI GTATCC 1 cut(s) 1232
BclI TGATCA 1 cut(s) 633
BcoDI GTCTC 3 cut(s) 75, 475, 1034
BfaI CTAG 1 cut(s) 959
BfmI CTRYAG 4 cut(s) 61, 231, 1006, 1227
BfuI GTATCC 1 cut(s) 1232
BglII AGATCT 2 cut(s) 961, 1105
BisI GCNGC 2 cut(s) 246, 1224
BlsI GCNGC 2 cut(s) 247, 1225
BmcAI AGTACT 1 cut(s) 20
Bme18I GGWCC 1 cut(s) 866
BmgT120I GGNCC 3 cut(s) 410, 866, 1192
BmiI GGNNCC 1 cut(s) 867
BmrI ACTGGG 1 cut(s) 31
BmsI GCATC 2 cut(s) 821, 1034
BmuI ACTGGG 1 cut(s) 31
BpiI GAAGAC 5 cut(s) 132, 361, 406, 773, 983
BplI GAGNNNNNCTC 2 cut(s) 698, 730
BpmI CTGGAG 1 cut(s) 337
Bpu14I TTCGAA 1 cut(s) 397
BsaBI GATNNNNATC 1 cut(s) 347
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 1234
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 466, 1201
Bse1I ACTGG 5 cut(s) 26, 138, 365, 412, 658
Bse8I GATNNNNATC 1 cut(s) 347
BseDI CCNNGG 1 cut(s) 1234
BseGI GGATG 8 cut(s) 16, 202, 322, 521, 836, 904, 1049, 1136
BseJI GATNNNNATC 1 cut(s) 347
BseLI CCNNNNNNNGG 3 cut(s) 66, 466, 1201
BseMII CTCAG 2 cut(s) 101, 1123
BseNI ACTGG 5 cut(s) 26, 138, 365, 412, 658
BseRI GAGGAG 1 cut(s) 75
BseXI GCAGC 2 cut(s) 257, 1210
BshFI GGCC 2 cut(s) 411, 1194
BsiHKAI GWGCWC 2 cut(s) 56, 240
BslFI GGGAC 2 cut(s) 879, 916
BslI CCNNNNNNNGG 3 cut(s) 66, 466, 1201
BsmAI GTCTC 3 cut(s) 75, 475, 1034
BsmFI GGGAC 2 cut(s) 879, 916
BsmI GAATGC 2 cut(s) 849, 1094
BsnI GGCC 2 cut(s) 411, 1194
Bso31I GGTCTC 1 cut(s) 75
Bsp119I TTCGAA 1 cut(s) 397
Bsp1286I GDGCHC 2 cut(s) 56, 240
Bsp143I GATC 4 cut(s) 382, 633, 961, 1105
BspANI GGCC 2 cut(s) 411, 1194
BspCNI CTCAG 2 cut(s) 100, 1122
BspHI TCATGA 2 cut(s) 73, 1206
BspLI GGNNCC 1 cut(s) 867
BspMAI CTGCAG 1 cut(s) 235
BspPI GGATC 1 cut(s) 377
BspT104I TTCGAA 1 cut(s) 397
BspTNI GGTCTC 1 cut(s) 75
BsrI ACTGG 5 cut(s) 26, 138, 365, 412, 658
BssECI CCNNGG 1 cut(s) 1234
BssMI GATC 4 cut(s) 382, 633, 961, 1105
BssSI CACGAG 1 cut(s) 130
Bst2BI CACGAG 1 cut(s) 130
Bst4CI ACNGT 3 cut(s) 65, 264, 764
BstAPI GCANNNNNTGC 1 cut(s) 1220
BstBI TTCGAA 1 cut(s) 397
BstC8I GCNNGC 1 cut(s) 1130
BstDEI CTNAG 6 cut(s) 87, 528, 614, 965, 1022, 1109
BstF5I GGATG 8 cut(s) 16, 202, 322, 521, 836, 904, 1049, 1136
BstHHI GCGC 1 cut(s) 1059
BstKTI GATC 4 cut(s) 385, 636, 964, 1108
BstMAI GTCTC 3 cut(s) 75, 475, 1034
BstMBI GATC 4 cut(s) 382, 633, 961, 1105
BstMWI GCNNNNNNNGC 2 cut(s) 1191, 1220
BstSFI CTRYAG 4 cut(s) 61, 231, 1006, 1227
BstV1I GCAGC 2 cut(s) 257, 1210
BstV2I GAAGAC 5 cut(s) 132, 361, 406, 773, 983
BstX2I RGATCY 2 cut(s) 961, 1105
BstYI RGATCY 2 cut(s) 961, 1105
BsuI GTATCC 1 cut(s) 1232
BsuRI GGCC 2 cut(s) 411, 1194
BtsCI GGATG 8 cut(s) 16, 202, 322, 521, 836, 904, 1049, 1136
BtsIMutI CAGTG 5 cut(s) 70, 95, 144, 651, 1117
Cac8I GCNNGC 1 cut(s) 1130
CciI TCATGA 2 cut(s) 73, 1206
CfoI GCGC 1 cut(s) 1059
Cfr13I GGNCC 3 cut(s) 410, 866, 1192
Csp6I GTAC 2 cut(s) 19, 97
CviAII CATG 7 cut(s) 74, 377, 437, 443, 1012, 1196, 1207
CviQI GTAC 2 cut(s) 19, 97
DdeI CTNAG 6 cut(s) 87, 528, 614, 965, 1022, 1109
DpnI GATC 4 cut(s) 384, 635, 963, 1107
DpnII GATC 4 cut(s) 382, 633, 961, 1105
DraIII CACNNNGTG 1 cut(s) 309
Ecl136II GAGCTC 1 cut(s) 238
Eco24I GRGCYC 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 866
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 1184
EcoICRI GAGCTC 1 cut(s) 238
EcoT38I GRGCYC 1 cut(s) 240
FaeI CATG 7 cut(s) 77, 380, 440, 446, 1015, 1199, 1210
FaqI GGGAC 2 cut(s) 879, 916
FatI CATG 7 cut(s) 73, 376, 436, 442, 1011, 1195, 1206
FbaI TGATCA 1 cut(s) 633
FblI GTMKAC 1 cut(s) 1230
Fnu4HI GCNGC 2 cut(s) 246, 1224
FokI GGATG 8 cut(s) 23, 209, 329, 528, 843, 911, 1056, 1123
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 2 cut(s) 246, 1224
FspBI CTAG 1 cut(s) 959
FspI TGCGCA 1 cut(s) 1058
GlaI GCGC 1 cut(s) 1058
GluI GCNGC 2 cut(s) 246, 1224
GsuI CTGGAG 1 cut(s) 337
HaeIII GGCC 2 cut(s) 411, 1194
HhaI GCGC 1 cut(s) 1059
Hin1II CATG 7 cut(s) 77, 380, 440, 446, 1015, 1199, 1210
Hin6I GCGC 1 cut(s) 1057
HinP1I GCGC 1 cut(s) 1057
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HindIII AAGCTT 1 cut(s) 737
HphI GGTGA 1 cut(s) 912
Hpy166II GTNNAC 2 cut(s) 222, 1231
Hpy188I TCNGA 6 cut(s) 46, 391, 996, 1152, 1164, 1238
Hpy8I GTNNAC 2 cut(s) 222, 1231
HpyAV CCTTC 4 cut(s) 46, 396, 436, 460
HpyCH4III ACNGT 3 cut(s) 65, 264, 764
HpyCH4IV ACGT 1 cut(s) 358
HpyCH4V TGCA 5 cut(s) 233, 693, 725, 975, 1121
HpyF10VI GCNNNNNNNGC 2 cut(s) 1191, 1220
HpyF3I CTNAG 6 cut(s) 87, 528, 614, 965, 1022, 1109
HpySE526I ACGT 1 cut(s) 358
Hsp92II CATG 7 cut(s) 77, 380, 440, 446, 1015, 1199, 1210
HspAI GCGC 1 cut(s) 1057
Ksp22I TGATCA 1 cut(s) 633
Kzo9I GATC 4 cut(s) 382, 633, 961, 1105
LmnI GCTCC 2 cut(s) 51, 88
Lsp1109I GCAGC 2 cut(s) 257, 1210
LweI GCATC 2 cut(s) 821, 1034
MaeI CTAG 1 cut(s) 959
MaeII ACGT 1 cut(s) 358
MaeIII GTNAC 1 cut(s) 593
MalI GATC 4 cut(s) 384, 635, 963, 1107
MboI GATC 4 cut(s) 382, 633, 961, 1105
MboII GAAGA 7 cut(s) 137, 291, 361, 411, 475, 773, 983
MfeI CAATTG 1 cut(s) 930
MflI RGATCY 2 cut(s) 961, 1105
MhlI GDGCHC 2 cut(s) 56, 240
MlyI GAGTC 2 cut(s) 41, 787
MnlI CCTC 9 cut(s) 67, 96, 170, 907, 933, 1013, 1150, 1183, 1244
MseI TTAA 5 cut(s) 252, 333, 741, 1124, 1251
MslI CAYNNNNRTG 1 cut(s) 441
MunI CAATTG 1 cut(s) 930
Mva1269I GAATGC 2 cut(s) 849, 1094
MwoI GCNNNNNNNGC 2 cut(s) 1191, 1220
NdeII GATC 4 cut(s) 382, 633, 961, 1105
NlaIII CATG 7 cut(s) 77, 380, 440, 446, 1015, 1199, 1210
NlaIV GGNNCC 1 cut(s) 867
NmeAIII GCCGAG 1 cut(s) 221
NsbI TGCGCA 1 cut(s) 1058
NspV TTCGAA 1 cut(s) 397
PagI TCATGA 2 cut(s) 73, 1206
PctI GAATGC 2 cut(s) 849, 1094
PfeI GAWTC 8 cut(s) 77, 180, 257, 348, 709, 803, 917, 943
PkrI GCNGC 2 cut(s) 247, 1225
PleI GAGTC 2 cut(s) 40, 786
PpsI GAGTC 2 cut(s) 40, 786
PshBI ATTAAT 1 cut(s) 333
Psp124BI GAGCTC 1 cut(s) 240
PspN4I GGNNCC 1 cut(s) 867
PspPI GGNCC 3 cut(s) 410, 866, 1192
PstI CTGCAG 1 cut(s) 235
PsuI RGATCY 2 cut(s) 961, 1105
RsaI GTAC 2 cut(s) 20, 98
RsaNI GTAC 2 cut(s) 19, 97
RseI CAYNNNNRTG 1 cut(s) 441
SacI GAGCTC 1 cut(s) 240
SaqAI TTAA 5 cut(s) 252, 333, 741, 1124, 1251
SatI GCNGC 2 cut(s) 246, 1224
Sau3AI GATC 4 cut(s) 382, 633, 961, 1105
Sau96I GGNCC 3 cut(s) 410, 866, 1192
ScaI AGTACT 1 cut(s) 20
SchI GAGTC 2 cut(s) 41, 787
SduI GDGCHC 2 cut(s) 56, 240
SfaNI GCATC 2 cut(s) 821, 1034
SfcI CTRYAG 4 cut(s) 61, 231, 1006, 1227
SfuI TTCGAA 1 cut(s) 397
SinI GGWCC 1 cut(s) 866
SmiMI CAYNNNNRTG 1 cut(s) 441
SspMI CTAG 1 cut(s) 959
SstI GAGCTC 1 cut(s) 240
TaaI ACNGT 3 cut(s) 65, 264, 764
TaiI ACGT 1 cut(s) 361
TaqI TCGA 2 cut(s) 341, 397
TatI WGTACW 1 cut(s) 18
TfiI GAWTC 8 cut(s) 77, 180, 257, 348, 709, 803, 917, 943
Tru1I TTAA 5 cut(s) 252, 333, 741, 1124, 1251
Tru9I TTAA 5 cut(s) 252, 333, 741, 1124, 1251
TscAI CASTG 5 cut(s) 70, 95, 151, 658, 1117
TseI GCWGC 2 cut(s) 245, 1223
TspDTI ATGAA 7 cut(s) 333, 453, 555, 887, 1028, 1169, 1223
TspRI CASTG 5 cut(s) 70, 95, 151, 658, 1117
VpaK11BI GGWCC 1 cut(s) 866
VspI ATTAAT 1 cut(s) 333
XapI RAATTY 6 cut(s) 628, 744, 751, 857, 877, 1061
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XmiI GTMKAC 1 cut(s) 1230
XspI CTAG 1 cut(s) 959
ZrmI AGTACT 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.