RLG00000036882

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
N/A
Physical Location & Seq
Forward (+)
86101356 .. 86103395
2040 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036882

Sequence Viewer

Length: 867 bp
ATGCCGCTCGACATAGAGCTCGTGAACGGCTCGTCGGAGTCGGTATCAAAACCAGAAACGCCGCTCGACGTGAAGCTCGTGAACGGCTCGTCGGAGGTGTCGTCCAGAGACTGGACCAAAATCTCTGCAGCCTTGTCCGAGTGGCCTTTGGCTTCGAGAAGCGCTAAAACGGCGTCGTCGACTGAGACCGAAGAGAAGGCCTTCGTCAGCGCGGCCACTGCCTTTTGCTCGGCGTCGTCTTTGGGGGGAGCGGCCAGCTTCGGATCTGCCGGCGAGCTCTTCTTCCTTCTCCTCCTCGTCTGCTTCTTCATCGTCAACGATAGGAACAGTTTCTTGTTCTTGCGGCCCTCCTCCTTCTGCTCGATGCGCTCCTCGACGGTGAGCTCGGACGCCGTGGTGGACCCATCTCCTCGTAGCGCTCCGCCTGCTCGGGTTGTCGATAAGAAAGGACGGCAATTATGTGAATTTAGGATTGATTTTCCTGGAAATTTCAAATGGGAGGACAAAGGATTGGCTTTCTGTGCTCTAACTATTGATTATAACTATGGGTTTACGATTGCATATATGAGAGTAATCACAATCTACATTAATGGGTTCGAAATTGAATTTGGAACCTACAATATACTGTCTGAGGAGATTACGGACGAAGGGTGCCCCATGCAAAGCTGCGGAGTCCACGTTGTAATGCCAGAGGATCATGAAGGCCCATTTGTAAATGGGACTGACTCCGACTATGATGCTGAAGAGATTGACTCCGACTACGATGCTGAAGAGATTATTGACTCCGACTACGATGCTGAAGATCGACTAACTGGTTTGATAGTAAGTCCTTGTCCTTCAGAGGGATATTGGTTTCTTGGCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

289

Amino Acids

31.48

Weight (kDa)

4.22

Isoelectric Point (pI)

53.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 540
AccB1I GGYRCC 1 cut(s) 651
AccB7I CCANNNNNTGG 1 cut(s) 111
AccBSI CCGCTC 3 cut(s) 7, 64, 251
AccI GTMKAC 1 cut(s) 179
AccII CGCG 1 cut(s) 212
AciI CCGC 7 cut(s) 5, 62, 212, 251, 343, 422, 669
AclWI GGATC 2 cut(s) 271, 702
AcoI YGGCCR 2 cut(s) 213, 252
AcsI RAATTY 3 cut(s) 464, 487, 605
AcuI CTGAAG 4 cut(s) 762, 789, 819, 822
AcyI GRCGYC 3 cut(s) 173, 233, 390
AfeI AGCGCT 2 cut(s) 163, 418
AfiI CCNNNNNNNGG 2 cut(s) 111, 842
AgsI TTSAA 2 cut(s) 493, 605
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 1 cut(s) 481
AjuI GAANNNNNNNTTGG 2 cut(s) 591, 623
AluBI AGCT 6 cut(s) 19, 76, 258, 277, 384, 666
AluI AGCT 6 cut(s) 19, 76, 258, 277, 384, 666
Alw21I GWGCWC 4 cut(s) 21, 279, 386, 526
Alw26I GTCTC 2 cut(s) 102, 179
AlwI GGATC 2 cut(s) 271, 702
AlwNI CAGNNNCTG 1 cut(s) 111
Ama87I CYCGRG 1 cut(s) 429
Aor51HI AGCGCT 2 cut(s) 163, 418
AoxI GGCC 6 cut(s) 143, 198, 213, 252, 344, 703
ApeKI GCWGC 2 cut(s) 128, 666
ApoI RAATTY 3 cut(s) 464, 487, 605
AseI ATTAAT 1 cut(s) 588
Asp700I GAANNNNTTC 2 cut(s) 200, 329
AspLEI GCGC 4 cut(s) 164, 212, 369, 419
AspS9I GGNCC 4 cut(s) 114, 345, 400, 704
AsuHPI GGTGA 1 cut(s) 391
AsuII TTCGAA 1 cut(s) 597
AvaI CYCGRG 1 cut(s) 429
AvaII GGWCC 2 cut(s) 114, 400
BaeGI GKGCMC 1 cut(s) 656
BanI GGYRCC 1 cut(s) 651
BanII GRGCYC 3 cut(s) 21, 279, 386
BauI CACGAG 2 cut(s) 20, 77
Bbv12I GWGCWC 4 cut(s) 21, 279, 386, 526
BbvI GCAGC 2 cut(s) 140, 653
BccI CCATC 1 cut(s) 412
BceAI ACGGC 5 cut(s) 43, 100, 186, 377, 467
BcgI CGANNNNNNTGC 6 cut(s) 719, 746, 753, 776, 780, 810
BciT130I CCWGG 1 cut(s) 483
BcoDI GTCTC 2 cut(s) 102, 179
BfmI CTRYAG 1 cut(s) 126
BfoI RGCGCY 2 cut(s) 165, 420
BisI GCNGC 7 cut(s) 5, 62, 129, 213, 252, 344, 667
BlsI GCNGC 7 cut(s) 6, 63, 130, 214, 253, 345, 668
Bme1390I CCNGG 1 cut(s) 483
Bme18I GGWCC 2 cut(s) 114, 400
BmeT110I CYCGRG 1 cut(s) 429
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 4 cut(s) 114, 345, 400, 704
BmiI GGNNCC 3 cut(s) 402, 613, 653
BmrFI CCNGG 1 cut(s) 483
BmsI GCATC 4 cut(s) 354, 727, 754, 784
BplI GAGNNNNNCTC 2 cut(s) 737, 769
Bpu14I TTCGAA 1 cut(s) 597
BsaHI GRCGYC 3 cut(s) 173, 233, 390
BsaI GGTCTC 1 cut(s) 179
BsaJI CCNNGG 1 cut(s) 393
BsaXI ACNNNNNCTCC 4 cut(s) 86, 116, 663, 693
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 842
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 2 cut(s) 116, 817
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 393
BseLI CCNNNNNNNGG 2 cut(s) 111, 842
BseMII CTCAG 2 cut(s) 174, 621
BseNI ACTGG 2 cut(s) 116, 817
BseRI GAGGAG 6 cut(s) 281, 284, 340, 361, 399, 647
BseSI GKGCMC 1 cut(s) 656
BseXI GCAGC 2 cut(s) 140, 653
Bsh1236I CGCG 1 cut(s) 212
BshFI GGCC 6 cut(s) 145, 200, 215, 254, 346, 705
BshNI GGYRCC 1 cut(s) 651
BsiHKAI GWGCWC 4 cut(s) 21, 279, 386, 526
BsiHKCI CYCGRG 1 cut(s) 429
BsiSI CCGG 1 cut(s) 270
BslFI GGGAC 1 cut(s) 733
BslI CCNNNNNNNGG 2 cut(s) 111, 842
BsmAI GTCTC 2 cut(s) 102, 179
BsmFI GGGAC 1 cut(s) 733
BsnI GGCC 6 cut(s) 145, 200, 215, 254, 346, 705
Bso31I GGTCTC 1 cut(s) 179
BsoBI CYCGRG 1 cut(s) 429
Bsp119I TTCGAA 1 cut(s) 597
Bsp1286I GDGCHC 5 cut(s) 21, 279, 386, 526, 656
Bsp143I GATC 3 cut(s) 263, 694, 802
BspACI CCGC 7 cut(s) 5, 62, 212, 251, 343, 422, 669
BspANI GGCC 6 cut(s) 145, 200, 215, 254, 346, 705
BspCNI CTCAG 2 cut(s) 175, 622
BspFNI CGCG 1 cut(s) 212
BspHI TCATGA 1 cut(s) 697
BspLI GGNNCC 3 cut(s) 402, 613, 653
BspMAI CTGCAG 1 cut(s) 130
BspPI GGATC 2 cut(s) 271, 702
BspQI GCTCTTC 1 cut(s) 284
BspT104I TTCGAA 1 cut(s) 597
BspT107I GGYRCC 1 cut(s) 651
BspTNI GGTCTC 1 cut(s) 179
BsrBI CCGCTC 3 cut(s) 7, 64, 251
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 2 cut(s) 116, 817
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 1 cut(s) 393
BssMI GATC 3 cut(s) 263, 694, 802
BssNI GRCGYC 3 cut(s) 173, 233, 390
BssSI CACGAG 2 cut(s) 20, 77
Bst2BI CACGAG 2 cut(s) 20, 77
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 3 cut(s) 329, 379, 627
Bst6I CTCTTC 4 cut(s) 186, 284, 738, 765
BstACI GRCGYC 3 cut(s) 173, 233, 390
BstBI TTCGAA 1 cut(s) 597
BstC8I GCNNGC 4 cut(s) 256, 271, 275, 426
BstDEI CTNAG 2 cut(s) 183, 630
BstDSI CCRYGG 1 cut(s) 393
BstFNI CGCG 1 cut(s) 212
BstH2I RGCGCY 2 cut(s) 165, 420
BstHHI GCGC 4 cut(s) 164, 212, 369, 419
BstKTI GATC 3 cut(s) 266, 697, 805
BstMAI GTCTC 2 cut(s) 102, 179
BstMBI GATC 3 cut(s) 263, 694, 802
BstMWI GCNNNNNNNGC 5 cut(s) 170, 218, 366, 425, 521
BstNI CCWGG 1 cut(s) 483
BstSCI CCNGG 1 cut(s) 481
BstSFI CTRYAG 1 cut(s) 126
BstSLI GKGCMC 1 cut(s) 656
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 2 cut(s) 140, 653
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BsuRI GGCC 6 cut(s) 145, 200, 215, 254, 346, 705
BtgI CCRYGG 1 cut(s) 393
BtrI CACGTC 1 cut(s) 70
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 1 cut(s) 216
Cac8I GCNNGC 4 cut(s) 256, 271, 275, 426
CaiI CAGNNNCTG 1 cut(s) 111
CciI TCATGA 1 cut(s) 697
CfoI GCGC 4 cut(s) 164, 212, 369, 419
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 4 cut(s) 114, 345, 400, 704
CseI GACGC 3 cut(s) 162, 222, 398
CviAII CATG 2 cut(s) 658, 698
DdeI CTNAG 2 cut(s) 183, 630
DpnI GATC 3 cut(s) 265, 696, 804
DpnII GATC 3 cut(s) 263, 694, 802
EaeI YGGCCR 2 cut(s) 213, 252
Eam1104I CTCTTC 4 cut(s) 186, 284, 738, 765
EarI CTCTTC 4 cut(s) 186, 284, 738, 765
EciI GGCGGA 1 cut(s) 411
Ecl136II GAGCTC 3 cut(s) 19, 277, 384
Eco147I AGGCCT 1 cut(s) 200
Eco24I GRGCYC 3 cut(s) 21, 279, 386
Eco31I GGTCTC 1 cut(s) 179
Eco47I GGWCC 2 cut(s) 114, 400
Eco47III AGCGCT 2 cut(s) 163, 418
Eco53kI GAGCTC 3 cut(s) 19, 277, 384
Eco57I CTGAAG 4 cut(s) 762, 789, 819, 822
Eco88I CYCGRG 1 cut(s) 429
EcoICRI GAGCTC 3 cut(s) 19, 277, 384
EcoRII CCWGG 1 cut(s) 481
EcoT38I GRGCYC 3 cut(s) 21, 279, 386
FaeI CATG 2 cut(s) 661, 701
FaqI GGGAC 1 cut(s) 733
FatI CATG 2 cut(s) 657, 697
FblI GTMKAC 1 cut(s) 179
Fnu4HI GCNGC 7 cut(s) 5, 62, 129, 213, 252, 344, 667
FriOI GRGCYC 3 cut(s) 21, 279, 386
Fsp4HI GCNGC 7 cut(s) 5, 62, 129, 213, 252, 344, 667
GlaI GCGC 4 cut(s) 163, 211, 368, 418
GluI GCNGC 7 cut(s) 5, 62, 129, 213, 252, 344, 667
HaeII RGCGCY 2 cut(s) 165, 420
HaeIII GGCC 6 cut(s) 145, 200, 215, 254, 346, 705
HapII CCGG 1 cut(s) 270
HgaI GACGC 3 cut(s) 162, 222, 398
HhaI GCGC 4 cut(s) 164, 212, 369, 419
Hin1I GRCGYC 3 cut(s) 173, 233, 390
Hin1II CATG 2 cut(s) 661, 701
Hin6I GCGC 4 cut(s) 162, 210, 367, 417
HinP1I GCGC 4 cut(s) 162, 210, 367, 417
HincII GTYRAC 2 cut(s) 180, 316
HindII GTYRAC 2 cut(s) 180, 316
HinfI GANTC 5 cut(s) 38, 672, 725, 752, 782
HpaII CCGG 1 cut(s) 270
HphI GGTGA 1 cut(s) 391
Hpy166II GTNNAC 7 cut(s) 25, 82, 180, 316, 400, 552, 676
Hpy188III TCNNGA 5 cut(s) 22, 79, 105, 156, 698
Hpy8I GTNNAC 7 cut(s) 25, 82, 180, 316, 400, 552, 676
Hpy99I CGWCG 7 cut(s) 37, 71, 94, 178, 181, 238, 379
HpyAV CCTTC 7 cut(s) 190, 211, 296, 364, 641, 695, 846
HpyCH4III ACNGT 3 cut(s) 329, 379, 627
HpyCH4IV ACGT 2 cut(s) 69, 678
HpyCH4V TGCA 3 cut(s) 128, 560, 661
HpyF10VI GCNNNNNNNGC 5 cut(s) 170, 218, 366, 425, 521
HpyF3I CTNAG 2 cut(s) 183, 630
HpySE526I ACGT 2 cut(s) 69, 678
Hsp92I GRCGYC 3 cut(s) 173, 233, 390
Hsp92II CATG 2 cut(s) 661, 701
HspAI GCGC 4 cut(s) 162, 210, 367, 417
KroI GCCGGC 1 cut(s) 269
KroNI GCCGGC 1 cut(s) 271
Kzo9I GATC 3 cut(s) 263, 694, 802
LguI GCTCTTC 1 cut(s) 284
LmnI GCTCC 3 cut(s) 248, 374, 424
Lsp1109I GCAGC 2 cut(s) 140, 653
LweI GCATC 4 cut(s) 354, 727, 754, 784
MaeII ACGT 2 cut(s) 69, 678
MalI GATC 3 cut(s) 265, 696, 804
MbiI CCGCTC 3 cut(s) 7, 64, 251
MboI GATC 3 cut(s) 263, 694, 802
MboII GAAGA 7 cut(s) 203, 271, 274, 298, 755, 782, 812
MflI RGATCY 1 cut(s) 263
MhlI GDGCHC 5 cut(s) 21, 279, 386, 526, 656
MluCI AATT 5 cut(s) 455, 464, 487, 600, 605
MlyI GAGTC 5 cut(s) 47, 681, 719, 746, 776
MmeI TCCRAC 5 cut(s) 15, 72, 753, 780, 810
MroNI GCCGGC 1 cut(s) 269
MroXI GAANNNNTTC 2 cut(s) 200, 329
MseI TTAA 2 cut(s) 588, 865
MspI CCGG 1 cut(s) 270
MspR9I CCNGG 1 cut(s) 483
MvaI CCWGG 1 cut(s) 483
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 5 cut(s) 170, 218, 366, 425, 521
NaeI GCCGGC 1 cut(s) 271
NdeII GATC 3 cut(s) 263, 694, 802
NgoMIV GCCGGC 1 cut(s) 269
NlaIII CATG 2 cut(s) 661, 701
NlaIV GGNNCC 3 cut(s) 402, 613, 653
NmeAIII GCCGAG 1 cut(s) 209
NspV TTCGAA 1 cut(s) 597
PagI TCATGA 1 cut(s) 697
PceI AGGCCT 1 cut(s) 200
PciSI GCTCTTC 1 cut(s) 284
PcsI WCGNNNNNNNCGW 3 cut(s) 75, 98, 176
PdiI GCCGGC 1 cut(s) 271
PdmI GAANNNNTTC 2 cut(s) 200, 329
PflMI CCANNNNNTGG 1 cut(s) 111
PfoI TCCNGGA 1 cut(s) 481
PkrI GCNGC 7 cut(s) 6, 63, 130, 214, 253, 345, 668
PleI GAGTC 5 cut(s) 46, 680, 719, 746, 776
PpsI GAGTC 5 cut(s) 46, 680, 719, 746, 776
PshBI ATTAAT 1 cut(s) 588
PsiI TTATAA 1 cut(s) 540
Psp124BI GAGCTC 3 cut(s) 21, 279, 386
Psp6I CCWGG 1 cut(s) 481
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 3 cut(s) 402, 613, 653
PspPI GGNCC 4 cut(s) 114, 345, 400, 704
PstI CTGCAG 1 cut(s) 130
PstNI CAGNNNCTG 1 cut(s) 111
PsuI RGATCY 1 cut(s) 263
SacI GAGCTC 3 cut(s) 21, 279, 386
SalI GTCGAC 1 cut(s) 178
SapI GCTCTTC 1 cut(s) 284
SaqAI TTAA 2 cut(s) 588, 865
SatI GCNGC 7 cut(s) 5, 62, 129, 213, 252, 344, 667
Sau3AI GATC 3 cut(s) 263, 694, 802
Sau96I GGNCC 4 cut(s) 114, 345, 400, 704
SchI GAGTC 5 cut(s) 47, 681, 719, 746, 776
ScrFI CCNGG 1 cut(s) 483
SduI GDGCHC 5 cut(s) 21, 279, 386, 526, 656
SfaNI GCATC 4 cut(s) 354, 727, 754, 784
SfcI CTRYAG 1 cut(s) 126
SfuI TTCGAA 1 cut(s) 597
SinI GGWCC 2 cut(s) 114, 400
Sse9I AATT 5 cut(s) 455, 464, 487, 600, 605
SseBI AGGCCT 1 cut(s) 200
SsiI CCGC 7 cut(s) 5, 62, 212, 251, 343, 422, 669
SstI GAGCTC 3 cut(s) 21, 279, 386
StuI AGGCCT 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 481
TaaI ACNGT 3 cut(s) 329, 379, 627
TaiI ACGT 2 cut(s) 72, 681
TaqI TCGA 9 cut(s) 9, 66, 155, 179, 362, 374, 438, 597, 805
TaqII GACCGA 1 cut(s) 203
TasI AATT 5 cut(s) 455, 464, 487, 600, 605
TauI GCSGC 5 cut(s) 7, 64, 215, 254, 346
Tru1I TTAA 2 cut(s) 588, 865
Tru9I TTAA 2 cut(s) 588, 865
TscAI CASTG 1 cut(s) 223
TseI GCWGC 2 cut(s) 128, 666
TspDTI ATGAA 2 cut(s) 298, 714
TspGWI ACGGA 1 cut(s) 656
TspRI CASTG 1 cut(s) 223
Van91I CCANNNNNTGG 1 cut(s) 111
VpaK11BI GGWCC 2 cut(s) 114, 400
VspI ATTAAT 1 cut(s) 588
XapI RAATTY 3 cut(s) 464, 487, 605
XmiI GTMKAC 1 cut(s) 179
XmnI GAANNNNTTC 2 cut(s) 200, 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.