Rmu_co8136354.1_g000001

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8136354.1
N/A
Physical Location & Seq
Reverse (-)
2 .. 398
397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8136354.1_g000001.1.cds

Sequence Viewer

Length: 397 bp
atggatgctgctagctcatcatcatcagctcaacaagcttcgtcttccagtattcctcctactcaattcacatatcaagtgttcttgagtttcagaggcagaaacactcgcaagactttcaccgatcatctctacacagccctgaccactgcaggaatctgcactttccgagacgacgacgagctcccggccggagaagccatcaagccagaactagagagagccatccagcaatcgcgatgttctgttattgtgttttcaaaagactacgcgtcgtccggatggtgcctggacgagcttgtgacgatccttggatgcaagaagaggtccggtcagttcgttttaccgatctactacgacgtcagcccatcccaggtgaggaagcggacggggagtc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

132

Amino Acids

14.58

Weight (kDa)

8.39

Isoelectric Point (pI)

55.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 363
AccB1I GGYRCC 1 cut(s) 285
AccII CGCG 2 cut(s) 238, 272
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 1 cut(s) 385
AclWI GGATC 1 cut(s) 301
AcoI YGGCCR 1 cut(s) 189
AcyI GRCGYC 1 cut(s) 360
AfiI CCNNNNNNNGG 2 cut(s) 373, 378
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 1 cut(s) 261
AhdI GACNNNNNGTC 2 cut(s) 271, 393
AjnI CCWGG 2 cut(s) 288, 372
AluBI AGCT 5 cut(s) 15, 29, 38, 184, 298
AluI AGCT 5 cut(s) 15, 29, 38, 184, 298
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 1 cut(s) 301
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 1 cut(s) 189
ApeKI GCWGC 1 cut(s) 8
AspS9I GGNCC 1 cut(s) 327
AsuC2I CCSGG 1 cut(s) 188
AsuHPI GGTGA 2 cut(s) 112, 388
AsuNHI GCTAGC 1 cut(s) 11
AvaII GGWCC 1 cut(s) 327
BanI GGYRCC 1 cut(s) 285
BanII GRGCYC 1 cut(s) 186
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 186
BccI CCATC 4 cut(s) 209, 233, 276, 376
BciT130I CCWGG 2 cut(s) 290, 374
BcnI CCSGG 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 2 cut(s) 12, 215
BfmI CTRYAG 1 cut(s) 150
BisI GCNGC 1 cut(s) 9
BlsI GCNGC 1 cut(s) 10
Bme1390I CCNGG 3 cut(s) 188, 290, 374
Bme18I GGWCC 1 cut(s) 327
BmeRI GACNNNNNGTC 2 cut(s) 271, 393
BmgT120I GGNCC 1 cut(s) 327
BmiI GGNNCC 1 cut(s) 287
BmrFI CCNGG 3 cut(s) 188, 290, 374
BmsI GCATC 1 cut(s) 305
BmtI GCTAGC 1 cut(s) 15
BpiI GAAGAC 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 106
BpuMI CCSGG 1 cut(s) 188
BsaHI GRCGYC 1 cut(s) 360
BsaJI CCNNGG 2 cut(s) 310, 372
BsaWI WCCGGW 2 cut(s) 278, 329
Bsc4I CCNNNNNNNGG 2 cut(s) 373, 378
Bse1I ACTGG 1 cut(s) 48
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 2 cut(s) 290, 374
BseDI CCNNGG 2 cut(s) 310, 372
BseGI GGATG 5 cut(s) 10, 225, 287, 320, 368
BseLI CCNNNNNNNGG 2 cut(s) 373, 378
BseNI ACTGG 1 cut(s) 48
BseX3I CGGCCG 1 cut(s) 189
BsgI GTGCAG 1 cut(s) 145
Bsh1236I CGCG 2 cut(s) 238, 272
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 1 cut(s) 191
BshNI GGYRCC 1 cut(s) 285
BsiEI CGRYCG 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 4 cut(s) 188, 192, 279, 330
BslI CCNNNNNNNGG 2 cut(s) 373, 378
BsmAI GTCTC 1 cut(s) 165
BsmBI CGTCTC 1 cut(s) 165
BsnI GGCC 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 186
Bsp13I TCCGGA 1 cut(s) 278
Bsp143I GATC 3 cut(s) 124, 306, 348
Bsp68I TCGCGA 1 cut(s) 238
BspACI CCGC 1 cut(s) 385
BspANI GGCC 1 cut(s) 191
BspEI TCCGGA 1 cut(s) 278
BspFNI CGCG 2 cut(s) 238, 272
BspLI GGNNCC 1 cut(s) 287
BspMAI CTGCAG 1 cut(s) 154
BspOI GCTAGC 1 cut(s) 15
BspPI GGATC 1 cut(s) 301
BspT107I GGYRCC 1 cut(s) 285
BsrI ACTGG 1 cut(s) 48
BssECI CCNNGG 2 cut(s) 310, 372
BssMI GATC 3 cut(s) 124, 306, 348
BssNI GRCGYC 1 cut(s) 360
BssT1I CCWWGG 1 cut(s) 310
Bst2UI CCWGG 2 cut(s) 290, 374
Bst6I CTCTTC 1 cut(s) 317
BstACI GRCGYC 1 cut(s) 360
BstC8I GCNNGC 1 cut(s) 13
BstF5I GGATG 5 cut(s) 10, 225, 287, 320, 368
BstFNI CGCG 2 cut(s) 238, 272
BstKTI GATC 3 cut(s) 127, 309, 351
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 3 cut(s) 124, 306, 348
BstMCI CGRYCG 1 cut(s) 192
BstMWI GCNNNNNNNGC 2 cut(s) 35, 197
BstNI CCWGG 2 cut(s) 290, 374
BstSCI CCNGG 3 cut(s) 186, 288, 372
BstSFI CTRYAG 1 cut(s) 150
BstUI CGCG 2 cut(s) 238, 272
BstV2I GAAGAC 1 cut(s) 36
BstZI CGGCCG 1 cut(s) 189
BsuRI GGCC 1 cut(s) 191
BtgZI GCGATG 1 cut(s) 253
BtsCI GGATG 5 cut(s) 10, 225, 287, 320, 368
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 147
BtuMI TCGCGA 1 cut(s) 238
Cac8I GCNNGC 1 cut(s) 13
Cfr13I GGNCC 1 cut(s) 327
CseI GACGC 1 cut(s) 261
DpnI GATC 3 cut(s) 126, 308, 350
DpnII GATC 3 cut(s) 124, 306, 348
DriI GACNNNNNGTC 2 cut(s) 271, 393
EaeI YGGCCR 1 cut(s) 189
EagI CGGCCG 1 cut(s) 189
Eam1104I CTCTTC 1 cut(s) 317
Eam1105I GACNNNNNGTC 2 cut(s) 271, 393
EarI CTCTTC 1 cut(s) 317
Ecl136II GAGCTC 1 cut(s) 184
EclXI CGGCCG 1 cut(s) 189
Eco130I CCWWGG 1 cut(s) 310
Eco24I GRGCYC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 327
Eco52I CGGCCG 1 cut(s) 189
Eco53kI GAGCTC 1 cut(s) 184
EcoICRI GAGCTC 1 cut(s) 184
EcoRII CCWGG 2 cut(s) 288, 372
EcoT14I CCWWGG 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 310
Esp3I CGTCTC 1 cut(s) 165
FaiI YATR 1 cut(s) 73
Fnu4HI GCNGC 1 cut(s) 9
FokI GGATG 5 cut(s) 17, 212, 294, 327, 355
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 1 cut(s) 9
FspBI CTAG 2 cut(s) 12, 215
GluI GCNGC 1 cut(s) 9
HaeIII GGCC 1 cut(s) 191
HapII CCGG 4 cut(s) 188, 192, 279, 330
HgaI GACGC 1 cut(s) 261
Hin1I GRCGYC 1 cut(s) 360
HindIII AAGCTT 1 cut(s) 36
HinfI GANTC 2 cut(s) 156, 394
HpaII CCGG 4 cut(s) 188, 192, 279, 330
HphI GGTGA 2 cut(s) 112, 388
Hpy188I TCNGA 2 cut(s) 95, 170
Hpy188III TCNNGA 3 cut(s) 85, 237, 279
Hpy99I CGWCG 4 cut(s) 179, 182, 277, 362
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 3 cut(s) 152, 162, 318
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 197
HpySE526I ACGT 1 cut(s) 360
Hsp92I GRCGYC 1 cut(s) 360
Kpn2I TCCGGA 1 cut(s) 278
Kzo9I GATC 3 cut(s) 124, 306, 348
LmnI GCTCC 1 cut(s) 189
LweI GCATC 1 cut(s) 305
MaeI CTAG 2 cut(s) 12, 215
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 1 cut(s) 301
MalI GATC 3 cut(s) 126, 308, 350
MboI GATC 3 cut(s) 124, 306, 348
MboII GAAGA 2 cut(s) 36, 334
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 1 cut(s) 65
MluI ACGCGT 1 cut(s) 270
MnlI CCTC 4 cut(s) 66, 89, 318, 372
MroI TCCGGA 1 cut(s) 278
MspI CCGG 4 cut(s) 188, 192, 279, 330
MspR9I CCNGG 3 cut(s) 188, 290, 374
MvaI CCWGG 2 cut(s) 290, 374
MvnI CGCG 2 cut(s) 238, 272
MwoI GCNNNNNNNGC 2 cut(s) 35, 197
NciI CCSGG 1 cut(s) 188
NdeII GATC 3 cut(s) 124, 306, 348
NheI GCTAGC 1 cut(s) 11
NlaIV GGNNCC 1 cut(s) 287
NmuCI GTSAC 1 cut(s) 301
NruI TCGCGA 1 cut(s) 238
PfeI GAWTC 1 cut(s) 156
PkrI GCNGC 1 cut(s) 10
Psp124BI GAGCTC 1 cut(s) 186
Psp6I CCWGG 2 cut(s) 288, 372
PspGI CCWGG 2 cut(s) 288, 372
PspN4I GGNNCC 1 cut(s) 287
PspPI GGNCC 1 cut(s) 327
PstI CTGCAG 1 cut(s) 154
RruI TCGCGA 1 cut(s) 238
SacI GAGCTC 1 cut(s) 186
SatI GCNGC 1 cut(s) 9
Sau3AI GATC 3 cut(s) 124, 306, 348
Sau96I GGNCC 1 cut(s) 327
ScrFI CCNGG 3 cut(s) 188, 290, 374
SduI GDGCHC 1 cut(s) 186
SetI ASST 8 cut(s) 17, 31, 40, 186, 300, 329, 363, 378
SfaNI GCATC 1 cut(s) 305
SfcI CTRYAG 1 cut(s) 150
SinI GGWCC 1 cut(s) 327
SmlI CTYRAG 1 cut(s) 85
SmoI CTYRAG 1 cut(s) 85
Sse9I AATT 1 cut(s) 65
SsiI CCGC 1 cut(s) 385
SspMI CTAG 2 cut(s) 12, 215
SstI GAGCTC 1 cut(s) 186
StyD4I CCNGG 3 cut(s) 186, 288, 372
StyI CCWWGG 1 cut(s) 310
TaiI ACGT 1 cut(s) 363
TasI AATT 1 cut(s) 65
TfiI GAWTC 1 cut(s) 156
TscAI CASTG 1 cut(s) 154
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 1 cut(s) 8
Tsp45I GTSAC 1 cut(s) 301
TspRI CASTG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 327
XspI CTAG 2 cut(s) 12, 215
ZraI GACGTC 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.