Rmu_sc0000124.1_g000047

Zinc knuckle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000124.1
N/A
Physical Location & Seq
Reverse (-)
193267 .. 193952
686 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 474 bp
atgaagaaggcagctttaggtctgaccaagaaggcacaggagagaggccaccgagaaaaccccatgggcagattgcctgggggtttctctttggctgagaaactgatcgagaacagtttattaatagctgcagaggaagcggcatcgatgcttgctctggcaaaggaagtcttggtgagcctagttgggggcggagatgcactccatgatccctacttttatctgtgtggccaactgttagctccgcgacgtcctatggctatcacatcatttgtagtagcgatcttaggagtgtggaggctcaaggaacgggttttgattcgtgaagagatggtaggagtctttgtgtttcagttcaagaaacaggaggagaaggatcacgtccttcacggtggtccttggttcttcaggggttccatgcttgttctggccgactatgacggtattggatcagtggaggatatctccctttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

157

Amino Acids

17.32

Weight (kDa)

6.91

Isoelectric Point (pI)

31.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 95 - 146 4.4e-08 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 253
AccII CGCG 1 cut(s) 247
AciI CCGC 3 cut(s) 140, 192, 245
AclWI GGATC 3 cut(s) 203, 384, 457
AcoI YGGCCR 2 cut(s) 229, 429
AcuI CTGAAG 1 cut(s) 391
AcyI GRCGYC 1 cut(s) 250
AfiI CCNNNNNNNGG 1 cut(s) 187
AgsI TTSAA 1 cut(s) 358
AjiI CACGTC 1 cut(s) 382
AjnI CCWGG 1 cut(s) 76
AluBI AGCT 3 cut(s) 14, 128, 242
AluI AGCT 3 cut(s) 14, 128, 242
AlwI GGATC 3 cut(s) 203, 384, 457
AoxI GGCC 3 cut(s) 46, 229, 429
ApeKI GCWGC 2 cut(s) 11, 128
AseI ATTAAT 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 395
AsuHPI GGTGA 1 cut(s) 187
AvaII GGWCC 1 cut(s) 395
BalI TGGCCA 1 cut(s) 231
BbvI GCAGC 2 cut(s) 23, 115
BccI CCATC 1 cut(s) 325
BciT130I CCWGG 1 cut(s) 78
BfaI CTAG 1 cut(s) 182
BfmI CTRYAG 1 cut(s) 129
BisI GCNGC 3 cut(s) 12, 129, 141
BlsI GCNGC 3 cut(s) 13, 130, 142
Bme1390I CCNGG 1 cut(s) 78
Bme18I GGWCC 1 cut(s) 395
BmgBI CACGTC 1 cut(s) 382
BmgT120I GGNCC 1 cut(s) 395
BmiI GGNNCC 1 cut(s) 415
BmrFI CCNGG 1 cut(s) 78
BmsI GCATC 3 cut(s) 138, 152, 187
BplI GAGNNNNNCTC 3 cut(s) 186, 218, 449
BpuEI CTTGAG 1 cut(s) 287
Bsa29I ATCGAT 1 cut(s) 146
BsaHI GRCGYC 1 cut(s) 250
BsaJI CCNNGG 3 cut(s) 63, 77, 398
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
Bsc4I CCNNNNNNNGG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 78
BseCI ATCGAT 1 cut(s) 146
BseDI CCNNGG 3 cut(s) 63, 77, 398
BseLI CCNNNNNNNGG 1 cut(s) 187
BseMII CTCAG 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 383
BseXI GCAGC 2 cut(s) 23, 115
Bsh1236I CGCG 1 cut(s) 247
BshFI GGCC 3 cut(s) 48, 231, 431
BshVI ATCGAT 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 187
BsnI GGCC 3 cut(s) 48, 231, 431
Bsp143I GATC 5 cut(s) 105, 208, 282, 376, 449
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 3 cut(s) 140, 192, 245
BspANI GGCC 3 cut(s) 48, 231, 431
BspCNI CTCAG 1 cut(s) 88
BspDI ATCGAT 1 cut(s) 146
BspFNI CGCG 1 cut(s) 247
BspLI GGNNCC 1 cut(s) 415
BspMAI CTGCAG 1 cut(s) 133
BspPI GGATC 3 cut(s) 203, 384, 457
BssECI CCNNGG 3 cut(s) 63, 77, 398
BssMI GATC 5 cut(s) 105, 208, 282, 376, 449
BssNI GRCGYC 1 cut(s) 250
BssT1I CCWWGG 2 cut(s) 63, 398
Bst2UI CCWGG 1 cut(s) 78
Bst4CI ACNGT 4 cut(s) 116, 237, 392, 443
Bst6I CTCTTC 1 cut(s) 321
BstACI GRCGYC 1 cut(s) 250
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 2 cut(s) 96, 286
BstDSI CCRYGG 1 cut(s) 63
BstFNI CGCG 1 cut(s) 247
BstKTI GATC 5 cut(s) 108, 211, 285, 379, 452
BstMBI GATC 5 cut(s) 105, 208, 282, 376, 449
BstMWI GCNNNNNNNGC 1 cut(s) 137
BstNI CCWGG 1 cut(s) 78
BstSCI CCNGG 1 cut(s) 76
BstSFI CTRYAG 1 cut(s) 129
BstUI CGCG 1 cut(s) 247
BstV1I GCAGC 2 cut(s) 23, 115
Bsu15I ATCGAT 1 cut(s) 146
BsuRI GGCC 3 cut(s) 48, 231, 431
BsuTUI ATCGAT 1 cut(s) 146
BtgI CCRYGG 1 cut(s) 63
BtrI CACGTC 1 cut(s) 382
BtsIMutI CAGTG 1 cut(s) 459
Cac8I GCNNGC 1 cut(s) 153
Cfr13I GGNCC 1 cut(s) 395
ClaI ATCGAT 1 cut(s) 146
CviAII CATG 3 cut(s) 64, 206, 418
DdeI CTNAG 2 cut(s) 96, 286
DpnI GATC 5 cut(s) 107, 210, 284, 378, 451
DpnII GATC 5 cut(s) 105, 208, 282, 376, 449
EaeI YGGCCR 2 cut(s) 229, 429
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
EciI GGCGGA 1 cut(s) 207
Eco130I CCWWGG 2 cut(s) 63, 398
Eco32I GATATC 1 cut(s) 463
Eco47I GGWCC 1 cut(s) 395
Eco57I CTGAAG 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 76
EcoRV GATATC 1 cut(s) 463
EcoT14I CCWWGG 2 cut(s) 63, 398
ErhI CCWWGG 2 cut(s) 63, 398
FaeI CATG 3 cut(s) 67, 209, 421
FaiI YATR 5 cut(s) 65, 207, 257, 419, 438
FalI AAGNNNNNCTT 2 cut(s) 155, 187
FatI CATG 3 cut(s) 63, 205, 417
Fnu4HI GCNGC 3 cut(s) 12, 129, 141
Fsp4HI GCNGC 3 cut(s) 12, 129, 141
FspBI CTAG 1 cut(s) 182
GluI GCNGC 3 cut(s) 12, 129, 141
HaeIII GGCC 3 cut(s) 48, 231, 431
Hin1I GRCGYC 1 cut(s) 250
Hin1II CATG 3 cut(s) 67, 209, 421
HinfI GANTC 2 cut(s) 319, 339
HphI GGTGA 1 cut(s) 187
Hpy188I TCNGA 1 cut(s) 24
Hpy188III TCNNGA 3 cut(s) 109, 323, 358
Hpy99I CGWCG 1 cut(s) 252
HpyAV CCTTC 3 cut(s) 25, 367, 395
HpyCH4III ACNGT 4 cut(s) 116, 237, 392, 443
HpyCH4IV ACGT 2 cut(s) 250, 381
HpyCH4V TGCA 2 cut(s) 131, 200
HpyF10VI GCNNNNNNNGC 1 cut(s) 137
HpyF3I CTNAG 2 cut(s) 96, 286
HpySE526I ACGT 2 cut(s) 250, 381
Hsp92I GRCGYC 1 cut(s) 250
Hsp92II CATG 3 cut(s) 67, 209, 421
Kzo9I GATC 5 cut(s) 105, 208, 282, 376, 449
LmnI GCTCC 1 cut(s) 247
LpnPI CCDG 7 cut(s) 23, 63, 90, 143, 350, 394, 413
Lsp1109I GCAGC 2 cut(s) 23, 115
LweI GCATC 3 cut(s) 138, 152, 187
MaeI CTAG 1 cut(s) 182
MaeII ACGT 2 cut(s) 250, 381
MalI GATC 5 cut(s) 107, 210, 284, 378, 451
MboI GATC 5 cut(s) 105, 208, 282, 376, 449
MboII GAAGA 3 cut(s) 16, 338, 397
MlsI TGGCCA 1 cut(s) 231
MluNI TGGCCA 1 cut(s) 231
MlyI GAGTC 1 cut(s) 348
MnlI CCTC 5 cut(s) 38, 127, 291, 361, 451
Mox20I TGGCCA 1 cut(s) 231
MscI TGGCCA 1 cut(s) 231
MseI TTAA 2 cut(s) 122, 472
Msp20I TGGCCA 1 cut(s) 231
MspR9I CCNGG 1 cut(s) 78
MvaI CCWGG 1 cut(s) 78
MvnI CGCG 1 cut(s) 247
MwoI GCNNNNNNNGC 1 cut(s) 137
NcoI CCATGG 1 cut(s) 63
NdeII GATC 5 cut(s) 105, 208, 282, 376, 449
NlaIII CATG 3 cut(s) 67, 209, 421
NlaIV GGNNCC 1 cut(s) 415
PfeI GAWTC 1 cut(s) 319
PkrI GCNGC 3 cut(s) 13, 130, 142
PleI GAGTC 1 cut(s) 347
PpsI GAGTC 1 cut(s) 347
PshBI ATTAAT 1 cut(s) 122
Psp6I CCWGG 1 cut(s) 76
PspGI CCWGG 1 cut(s) 76
PspN4I GGNNCC 1 cut(s) 415
PspPI GGNCC 1 cut(s) 395
PstI CTGCAG 1 cut(s) 133
SaqAI TTAA 2 cut(s) 122, 472
SatI GCNGC 3 cut(s) 12, 129, 141
Sau3AI GATC 5 cut(s) 105, 208, 282, 376, 449
Sau96I GGNCC 1 cut(s) 395
SchI GAGTC 1 cut(s) 348
ScrFI CCNGG 1 cut(s) 78
SetI ASST 6 cut(s) 16, 22, 130, 244, 253, 384
SfaNI GCATC 3 cut(s) 138, 152, 187
SfcI CTRYAG 1 cut(s) 129
SinI GGWCC 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SsiI CCGC 3 cut(s) 140, 192, 245
SspMI CTAG 1 cut(s) 182
StyD4I CCNGG 1 cut(s) 76
StyI CCWWGG 2 cut(s) 63, 398
TaaI ACNGT 4 cut(s) 116, 237, 392, 443
TaiI ACGT 2 cut(s) 253, 384
TaqI TCGA 2 cut(s) 108, 146
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 319
Tru1I TTAA 2 cut(s) 122, 472
Tru9I TTAA 2 cut(s) 122, 472
TscAI CASTG 1 cut(s) 459
TseI GCWGC 2 cut(s) 11, 128
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 459
VpaK11BI GGWCC 1 cut(s) 395
VspI ATTAAT 1 cut(s) 122
XcmI CCANNNNNNNNNTGG 1 cut(s) 424
XspI CTAG 1 cut(s) 182
ZraI GACGTC 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.