Rmu_sc0000218.1_g000021

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000218.1
N/A
Physical Location & Seq
Reverse (-)
78232 .. 82707
4476 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 387 bp
atgggattggaatatacttcaccagcttttacatctgccatggccaacatgactccatcgattaccttcatcttagctgttttgtgcaggatggaacagtttgatatttccaagcctggtacccaagccaagattggaggaactcttgctgcctttgctggtgcaacactcatgaccctctacaagggcgttgcttttcatcttaatcaaaacgagctgctttcccttcttgtacttctctcactttcttcaaaattgtggactttttcggttccactaaagatcatggaagactgtgttgaagacgctaatggctttgctcacggttccgaggaggtggaggagctctccgttcccagaggtgggcccctctacgcgagaatctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

128

Amino Acids

13.8

Weight (kDa)

5.03

Isoelectric Point (pI)

28.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 119
AccII CGCG 1 cut(s) 377
AcoI YGGCCR 1 cut(s) 42
AfaI GTAC 2 cut(s) 121, 234
AfiI CCNNNNNNNGG 3 cut(s) 184, 362, 363
AgsI TTSAA 2 cut(s) 252, 302
AjnI CCWGG 1 cut(s) 115
AluBI AGCT 4 cut(s) 26, 77, 217, 346
AluI AGCT 4 cut(s) 26, 77, 217, 346
Alw21I GWGCWC 1 cut(s) 348
AoxI GGCC 2 cut(s) 42, 365
ApaI GGGCCC 1 cut(s) 369
ApeKI GCWGC 2 cut(s) 149, 217
Asp718I GGTACC 1 cut(s) 119
AspS9I GGNCC 2 cut(s) 365, 366
AsuHPI GGTGA 1 cut(s) 12
BaeGI GKGCMC 1 cut(s) 369
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 1 cut(s) 119
BanII GRGCYC 2 cut(s) 348, 369
BbsI GAAGAC 2 cut(s) 297, 309
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 2 cut(s) 136, 204
BccI CCATC 2 cut(s) 64, 85
BciT130I CCWGG 1 cut(s) 117
BfaI CTAG 1 cut(s) 385
BisI GCNGC 2 cut(s) 150, 218
BlsI GCNGC 2 cut(s) 151, 219
Bme1390I CCNGG 1 cut(s) 117
BmgT120I GGNCC 2 cut(s) 365, 366
BmiI GGNNCC 5 cut(s) 121, 273, 328, 367, 368
BmrFI CCNGG 1 cut(s) 117
BpiI GAAGAC 2 cut(s) 297, 309
BplI GAGNNNNNCTC 2 cut(s) 332, 364
Bsa29I ATCGAT 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 39, 330
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 362, 363
BseBI CCWGG 1 cut(s) 117
BseCI ATCGAT 1 cut(s) 59
BseDI CCNNGG 2 cut(s) 39, 330
BseGI GGATG 1 cut(s) 96
BseLI CCNNNNNNNGG 3 cut(s) 184, 362, 363
BseRI GAGGAG 2 cut(s) 347, 356
BseSI GKGCMC 1 cut(s) 369
BseXI GCAGC 2 cut(s) 136, 204
BsgI GTGCAG 1 cut(s) 106
Bsh1236I CGCG 1 cut(s) 377
BshFI GGCC 2 cut(s) 44, 367
BshNI GGYRCC 1 cut(s) 119
BshVI ATCGAT 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 348
BslI CCNNNNNNNGG 3 cut(s) 184, 362, 363
BsnI GGCC 2 cut(s) 44, 367
Bsp120I GGGCCC 1 cut(s) 365
Bsp1286I GDGCHC 2 cut(s) 348, 369
Bsp143I GATC 1 cut(s) 282
Bsp19I CCATGG 1 cut(s) 39
BspANI GGCC 2 cut(s) 44, 367
BspDI ATCGAT 1 cut(s) 59
BspFNI CGCG 1 cut(s) 377
BspHI TCATGA 1 cut(s) 171
BspLI GGNNCC 5 cut(s) 121, 273, 328, 367, 368
BspT107I GGYRCC 1 cut(s) 119
BssECI CCNNGG 2 cut(s) 39, 330
BssMI GATC 1 cut(s) 282
BssT1I CCWWGG 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 3 cut(s) 99, 296, 326
BstDEI CTNAG 1 cut(s) 73
BstDSI CCRYGG 1 cut(s) 39
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 1 cut(s) 96
BstFNI CGCG 1 cut(s) 377
BstKTI GATC 1 cut(s) 285
BstMBI GATC 1 cut(s) 282
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstNI CCWGG 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 115
BstSLI GKGCMC 1 cut(s) 369
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 2 cut(s) 136, 204
BstV2I GAAGAC 2 cut(s) 297, 309
Bsu15I ATCGAT 1 cut(s) 59
BsuRI GGCC 2 cut(s) 44, 367
BsuTUI ATCGAT 1 cut(s) 59
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 96
CciI TCATGA 1 cut(s) 171
Cfr13I GGNCC 2 cut(s) 365, 366
ClaI ATCGAT 1 cut(s) 59
CseI GACGC 1 cut(s) 314
Csp6I GTAC 2 cut(s) 120, 233
CviAII CATG 4 cut(s) 40, 49, 172, 286
CviJI RGCY 9 cut(s) 26, 44, 77, 115, 128, 217, 315, 346, 367
CviKI_1 RGCY 9 cut(s) 26, 44, 77, 115, 128, 217, 315, 346, 367
CviQI GTAC 2 cut(s) 120, 233
DdeI CTNAG 1 cut(s) 73
DpnI GATC 1 cut(s) 284
DpnII GATC 1 cut(s) 282
EaeI YGGCCR 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 2 cut(s) 348, 369
Eco53kI GAGCTC 1 cut(s) 346
EcoICRI GAGCTC 1 cut(s) 346
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 366
EcoRII CCWGG 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 39
EcoT38I GRGCYC 2 cut(s) 348, 369
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 4 cut(s) 43, 52, 175, 289
FaiI YATR 5 cut(s) 15, 41, 50, 173, 287
FatI CATG 4 cut(s) 39, 48, 171, 285
Fnu4HI GCNGC 2 cut(s) 150, 218
FokI GGATG 1 cut(s) 103
FriOI GRGCYC 2 cut(s) 348, 369
Fsp4HI GCNGC 2 cut(s) 150, 218
FspBI CTAG 1 cut(s) 385
GluI GCNGC 2 cut(s) 150, 218
HaeIII GGCC 2 cut(s) 44, 367
HgaI GACGC 1 cut(s) 314
Hin1II CATG 4 cut(s) 43, 52, 175, 289
HinfI GANTC 2 cut(s) 52, 381
HphI GGTGA 1 cut(s) 12
Hpy166II GTNNAC 1 cut(s) 261
Hpy188I TCNGA 1 cut(s) 331
Hpy188III TCNNGA 1 cut(s) 172
Hpy8I GTNNAC 1 cut(s) 261
HpyAV CCTTC 2 cut(s) 76, 236
HpyCH4III ACNGT 3 cut(s) 99, 296, 326
HpyCH4V TGCA 2 cut(s) 87, 164
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 1 cut(s) 73
Hsp92II CATG 4 cut(s) 43, 52, 175, 289
KpnI GGTACC 1 cut(s) 123
Kzo9I GATC 1 cut(s) 282
LmnI GCTCC 1 cut(s) 343
LpnPI CCDG 6 cut(s) 36, 73, 102, 129, 144, 370
Lsp1109I GCAGC 2 cut(s) 136, 204
MaeI CTAG 1 cut(s) 385
MalI GATC 1 cut(s) 284
MboI GATC 1 cut(s) 282
MboII GAAGA 3 cut(s) 240, 302, 314
MhlI GDGCHC 2 cut(s) 348, 369
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 1 cut(s) 254
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 1 cut(s) 46
MnlI CCTC 7 cut(s) 131, 188, 325, 328, 334, 353, 380
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MseI TTAA 1 cut(s) 204
Msp20I TGGCCA 1 cut(s) 44
MspR9I CCNGG 1 cut(s) 117
MvaI CCWGG 1 cut(s) 117
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 1 cut(s) 155
NcoI CCATGG 1 cut(s) 39
NdeII GATC 1 cut(s) 282
NlaIII CATG 4 cut(s) 43, 52, 175, 289
NlaIV GGNNCC 5 cut(s) 121, 273, 328, 367, 368
PagI TCATGA 1 cut(s) 171
PfeI GAWTC 1 cut(s) 381
PkrI GCNGC 2 cut(s) 151, 219
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
Psp124BI GAGCTC 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 5 cut(s) 121, 273, 328, 367, 368
PspOMI GGGCCC 1 cut(s) 365
PspPI GGNCC 2 cut(s) 365, 366
RsaI GTAC 2 cut(s) 121, 234
RsaNI GTAC 2 cut(s) 120, 233
SacI GAGCTC 1 cut(s) 348
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 2 cut(s) 150, 218
Sau3AI GATC 1 cut(s) 282
Sau96I GGNCC 2 cut(s) 365, 366
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 2 cut(s) 348, 369
SetI ASST 7 cut(s) 28, 68, 79, 219, 339, 348, 364
Sse9I AATT 1 cut(s) 254
SspMI CTAG 1 cut(s) 385
SstI GAGCTC 1 cut(s) 348
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 3 cut(s) 99, 296, 326
TaqI TCGA 1 cut(s) 59
TasI AATT 1 cut(s) 254
TatI WGTACW 1 cut(s) 232
TfiI GAWTC 1 cut(s) 381
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TseI GCWGC 2 cut(s) 149, 217
TspDTI ATGAA 2 cut(s) 58, 188
TspGWI ACGGA 1 cut(s) 340
XagI CCTNNNNNAGG 1 cut(s) 182
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XspI CTAG 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.