Rmu_sc0000383.1_g000015

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000383.1
N/A
Physical Location & Seq
Forward (+)
69925 .. 71794
1870 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 447 bp
atgaatgttgggtatccaatgtgggatagctacccagtttatgcagctaaaccaaagaaagaagagaaggttggccttgcagaagccataacgaggagcatcctttcaacaggacgtttagacctccaaagaaagttgttttccagtatacaattgattggaggagtggctttaacaagaggtctcattcctgcagtggaggagagggttttacttgcaattccttcaactgaagcaatagatagggttgaggtcttgcaatcgagaggcaatccaacttttgtaccatggaaaggtggagcgatccttggaattcttgactttgctcgagatgggtggctacgtcgtgatgagtggattcaaaatggagttcgcgttggaaagggaagaaagtacaaggactcccatttcctcgaagcacaggcaatgtattatattaattcataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

148

Amino Acids

16.61

Weight (kDa)

9.55

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 17 - 126 1.1e-09 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 148
AccII CGCG 1 cut(s) 375
AclWI GGATC 1 cut(s) 298
AcsI RAATTY 1 cut(s) 312
AcuI CTGAAG 1 cut(s) 252
AfaI GTAC 2 cut(s) 285, 395
AfiI CCNNNNNNNGG 2 cut(s) 93, 293
AgsI TTSAA 3 cut(s) 108, 228, 362
AjuI GAANNNNNNNTTGG 2 cut(s) 54, 86
AluBI AGCT 2 cut(s) 30, 47
AluI AGCT 2 cut(s) 30, 47
Alw26I GTCTC 1 cut(s) 188
AlwI GGATC 1 cut(s) 298
Ama87I CYCGRG 1 cut(s) 327
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 1 cut(s) 44
ApoI RAATTY 1 cut(s) 312
AseI ATTAAT 1 cut(s) 438
AvaI CYCGRG 1 cut(s) 327
BaeI ACNNNNGTAYC 2 cut(s) 267, 300
BbvI GCAGC 1 cut(s) 56
BccI CCATC 1 cut(s) 326
BciVI GTATCC 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 188
BfmI CTRYAG 1 cut(s) 192
BfuI GTATCC 1 cut(s) 24
BisI GCNGC 1 cut(s) 45
BlsI GCNGC 1 cut(s) 46
BmeT110I CYCGRG 1 cut(s) 327
BmrI ACTGGG 1 cut(s) 29
BmsI GCATC 1 cut(s) 108
BmuI ACTGGG 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 2 cut(s) 287, 307
BsaXI ACNNNNNCTCC 4 cut(s) 360, 386, 390, 416
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 293
Bse1I ACTGG 2 cut(s) 35, 144
Bse3DI GCAATG 1 cut(s) 432
BseDI CCNNGG 2 cut(s) 287, 307
BseGI GGATG 1 cut(s) 99
BseLI CCNNNNNNNGG 2 cut(s) 93, 293
BseMI GCAATG 1 cut(s) 432
BseNI ACTGG 2 cut(s) 35, 144
BseRI GAGGAG 3 cut(s) 109, 177, 215
BseXI GCAGC 1 cut(s) 56
Bsh1236I CGCG 1 cut(s) 375
BshFI GGCC 1 cut(s) 75
BsiHKCI CYCGRG 1 cut(s) 327
BslI CCNNNNNNNGG 2 cut(s) 93, 293
BsmAI GTCTC 1 cut(s) 188
BsnI GGCC 1 cut(s) 75
Bso31I GGTCTC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 327
Bsp143I GATC 1 cut(s) 303
Bsp19I CCATGG 1 cut(s) 287
BspANI GGCC 1 cut(s) 75
BspFNI CGCG 1 cut(s) 375
BspMAI CTGCAG 1 cut(s) 196
BspPI GGATC 1 cut(s) 298
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 432
BsrI ACTGG 2 cut(s) 35, 144
BssECI CCNNGG 2 cut(s) 287, 307
BssMI GATC 1 cut(s) 303
BssNAI GTATAC 1 cut(s) 149
BssT1I CCWWGG 2 cut(s) 287, 307
Bst1107I GTATAC 1 cut(s) 149
Bst6I CTCTTC 1 cut(s) 57
BstDSI CCRYGG 1 cut(s) 287
BstF5I GGATG 1 cut(s) 99
BstFNI CGCG 1 cut(s) 375
BstKTI GATC 1 cut(s) 306
BstMAI GTCTC 1 cut(s) 188
BstMBI GATC 1 cut(s) 303
BstSFI CTRYAG 1 cut(s) 192
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 56
BstZ17I GTATAC 1 cut(s) 149
BsuI GTATCC 1 cut(s) 24
BsuRI GGCC 1 cut(s) 75
BtgI CCRYGG 1 cut(s) 287
BtsCI GGATG 1 cut(s) 99
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 1 cut(s) 201
Csp6I GTAC 2 cut(s) 284, 394
CviAII CATG 1 cut(s) 288
CviJI RGCY 6 cut(s) 30, 47, 75, 86, 170, 340
CviKI_1 RGCY 6 cut(s) 30, 47, 75, 86, 170, 340
CviQI GTAC 2 cut(s) 284, 394
DpnI GATC 1 cut(s) 305
DpnII GATC 1 cut(s) 303
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Eco130I CCWWGG 2 cut(s) 287, 307
Eco31I GGTCTC 1 cut(s) 188
Eco57I CTGAAG 1 cut(s) 252
Eco88I CYCGRG 1 cut(s) 327
EcoRI GAATTC 1 cut(s) 312
EcoT14I CCWWGG 2 cut(s) 287, 307
ErhI CCWWGG 2 cut(s) 287, 307
FaeI CATG 1 cut(s) 291
FaiI YATR 6 cut(s) 42, 89, 149, 289, 435, 445
FatI CATG 1 cut(s) 287
FblI GTMKAC 1 cut(s) 148
Fnu4HI GCNGC 1 cut(s) 45
FokI GGATG 1 cut(s) 86
Fsp4HI GCNGC 1 cut(s) 45
GluI GCNGC 1 cut(s) 45
HaeIII GGCC 1 cut(s) 75
Hin1II CATG 1 cut(s) 291
HinfI GANTC 2 cut(s) 358, 401
Hpy166II GTNNAC 1 cut(s) 149
Hpy188III TCNNGA 4 cut(s) 264, 317, 329, 347
Hpy8I GTNNAC 1 cut(s) 149
Hpy99I CGWCG 1 cut(s) 348
HpyAV CCTTC 2 cut(s) 61, 234
HpyCH4IV ACGT 2 cut(s) 115, 343
HpyCH4V TGCA 5 cut(s) 44, 80, 194, 218, 259
HpySE526I ACGT 2 cut(s) 115, 343
Hsp92II CATG 1 cut(s) 291
Kzo9I GATC 1 cut(s) 303
LmnI GCTCC 2 cut(s) 96, 299
LpnPI CCDG 5 cut(s) 48, 96, 157, 204, 407
Lsp1109I GCAGC 1 cut(s) 56
LweI GCATC 1 cut(s) 108
MaeII ACGT 2 cut(s) 115, 343
MalI GATC 1 cut(s) 305
MboI GATC 1 cut(s) 303
MboII GAAGA 2 cut(s) 74, 399
MfeI CAATTG 1 cut(s) 152
MluCI AATT 4 cut(s) 152, 219, 312, 439
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 2 cut(s) 299, 358
MnlI CCTC 9 cut(s) 87, 134, 155, 173, 193, 198, 244, 260, 422
MseI TTAA 2 cut(s) 173, 438
MunI CAATTG 1 cut(s) 152
MvnI CGCG 1 cut(s) 375
NcoI CCATGG 1 cut(s) 287
NdeII GATC 1 cut(s) 303
NlaIII CATG 1 cut(s) 291
PaeR7I CTCGAG 1 cut(s) 327
PfeI GAWTC 1 cut(s) 358
PkrI GCNGC 1 cut(s) 46
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
PshBI ATTAAT 1 cut(s) 438
PstI CTGCAG 1 cut(s) 196
RsaI GTAC 2 cut(s) 285, 395
RsaNI GTAC 2 cut(s) 284, 394
SaqAI TTAA 2 cut(s) 173, 438
SatI GCNGC 1 cut(s) 45
Sau3AI GATC 1 cut(s) 303
SchI GAGTC 1 cut(s) 395
SetI ASST 9 cut(s) 32, 49, 72, 118, 126, 184, 255, 298, 346
SfaNI GCATC 1 cut(s) 108
SfcI CTRYAG 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 327
SlaI CTCGAG 1 cut(s) 327
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
Sse9I AATT 4 cut(s) 152, 219, 312, 439
StyI CCWWGG 2 cut(s) 287, 307
TaiI ACGT 2 cut(s) 118, 346
TaqI TCGA 3 cut(s) 263, 328, 414
TasI AATT 4 cut(s) 152, 219, 312, 439
TatI WGTACW 1 cut(s) 393
TfiI GAWTC 1 cut(s) 358
Tru1I TTAA 2 cut(s) 173, 438
Tru9I TTAA 2 cut(s) 173, 438
TscAI CASTG 1 cut(s) 201
TseI GCWGC 1 cut(s) 44
TspDTI ATGAA 2 cut(s) 17, 432
TspRI CASTG 1 cut(s) 201
VspI ATTAAT 1 cut(s) 438
XapI RAATTY 1 cut(s) 312
XhoI CTCGAG 1 cut(s) 327
XmiI GTMKAC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.