Rmu_sc0000411.1_g000008

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000411.1
OG0031071
Physical Location & Seq
Reverse (-)
35511 .. 36342
832 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 591 bp
atggtagaccttcagcagcagagctccgacgttgtcgccgtcgccgacccttcccgcaacctttggttccgcctctacactcccacctccgcgactaccaccaagctccccatcatcgtcttcttccacggcggcgggtttgtattcatgtccgccacttcaaaaccctacgacgacttctgtcaacgcctcgctctccagctccccgccgtcgtcatctccttcaaactaccgcctcgcgccggagcatcgagcgagcgggcacgagtttcgggatctgaaagtggtcagaatgttggcgattcagccgtttttcggcggagaagagcggacagaatcggagacgagactgaagagagtgccgctggtgaacgtagagcggacggactggatgtggaaggcgtttttgccggtggggtcggatcggtggtgaatgtgttcggttcgaaagcggtggattcgatcccttactacgagcgattgaagaaacgcgggaaacaagcggagctggcggtgcttatcctgagttggatgactcttcttcgcctgaatggttttaaaagagacaaatcacaatttgggattaactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

196

Amino Acids

21.42

Weight (kDa)

9.51

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 39 - 75 8.9e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031071)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000411.1_g000008 Rmu_sc0001209.1_g000041

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 259, 329, 380
AccI GTMKAC 1 cut(s) 6
AccII CGCG 3 cut(s) 92, 240, 492
AclWI GGATC 3 cut(s) 283, 430, 457
AcuI CTGAAG 1 cut(s) 372
AfiI CCNNNNNNNGG 2 cut(s) 242, 315
AgsI TTSAA 3 cut(s) 162, 226, 484
AluBI AGCT 4 cut(s) 24, 106, 202, 508
AluI AGCT 4 cut(s) 24, 106, 202, 508
Alw21I GWGCWC 1 cut(s) 26
Alw26I GTCTC 3 cut(s) 336, 341, 558
AlwI GGATC 3 cut(s) 283, 430, 457
ApeKI GCWGC 1 cut(s) 16
Asp700I GAANNNNTTC 1 cut(s) 437
AspLEI GCGC 1 cut(s) 242
AsuHPI GGTGA 2 cut(s) 380, 442
AsuII TTCGAA 1 cut(s) 446
BaeGI GKGCMC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 26
BauI CACGAG 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 1 cut(s) 26
BbvI GCAGC 1 cut(s) 28
BccI CCATC 1 cut(s) 119
BceAI ACGGC 4 cut(s) 23, 145, 194, 293
BcgI CGANNNNNNTGC 2 cut(s) 252, 286
BcoDI GTCTC 3 cut(s) 336, 341, 558
BisI GCNGC 3 cut(s) 17, 133, 363
BlsI GCNGC 3 cut(s) 18, 134, 364
BmiI GGNNCC 1 cut(s) 68
BmsI GCATC 1 cut(s) 257
BoxI GACNNNNGTC 1 cut(s) 180
BpiI GAAGAC 1 cut(s) 112
BpmI CTGGAG 1 cut(s) 182
Bpu14I TTCGAA 1 cut(s) 446
BsaJI CCNNGG 1 cut(s) 127
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 315
Bse118I RCCGGY 1 cut(s) 410
Bse1I ACTGG 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 127
BseGI GGATG 2 cut(s) 397, 537
BseLI CCNNNNNNNGG 2 cut(s) 242, 315
BseMII CTCAG 1 cut(s) 515
BseNI ACTGG 1 cut(s) 393
BseSI GKGCMC 1 cut(s) 265
BseXI GCAGC 1 cut(s) 28
Bsh1236I CGCG 3 cut(s) 92, 240, 492
BsiHKAI GWGCWC 1 cut(s) 26
BsiSI CCGG 2 cut(s) 243, 411
BslI CCNNNNNNNGG 2 cut(s) 242, 315
BsmAI GTCTC 3 cut(s) 336, 341, 558
BsmBI CGTCTC 1 cut(s) 336
Bsp119I TTCGAA 1 cut(s) 446
Bsp1286I GDGCHC 2 cut(s) 26, 265
Bsp143I GATC 3 cut(s) 275, 422, 462
BspCNI CTCAG 1 cut(s) 516
BspFNI CGCG 3 cut(s) 92, 240, 492
BspLI GGNNCC 1 cut(s) 68
BspPI GGATC 3 cut(s) 283, 430, 457
BspQI GCTCTTC 1 cut(s) 319
BspT104I TTCGAA 1 cut(s) 446
BsrBI CCGCTC 3 cut(s) 259, 329, 380
BsrFI RCCGGY 1 cut(s) 410
BsrI ACTGG 1 cut(s) 393
BssAI RCCGGY 1 cut(s) 410
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 3 cut(s) 275, 422, 462
BssSI CACGAG 1 cut(s) 264
Bst2BI CACGAG 1 cut(s) 264
Bst6I CTCTTC 3 cut(s) 319, 348, 543
BstBI TTCGAA 1 cut(s) 446
BstC8I GCNNGC 3 cut(s) 257, 261, 510
BstDEI CTNAG 1 cut(s) 524
BstDSI CCRYGG 1 cut(s) 127
BstF5I GGATG 2 cut(s) 397, 537
BstFNI CGCG 3 cut(s) 92, 240, 492
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 3 cut(s) 278, 425, 465
BstMAI GTCTC 3 cut(s) 336, 341, 558
BstMBI GATC 3 cut(s) 275, 422, 462
BstMWI GCNNNNNNNGC 2 cut(s) 509, 514
BstPAI GACNNNNGTC 1 cut(s) 180
BstSLI GKGCMC 1 cut(s) 265
BstUI CGCG 3 cut(s) 92, 240, 492
BstV1I GCAGC 1 cut(s) 28
BstV2I GAAGAC 1 cut(s) 112
BstX2I RGATCY 1 cut(s) 275
BstYI RGATCY 1 cut(s) 275
BtgI CCRYGG 1 cut(s) 127
BtsCI GGATG 2 cut(s) 397, 537
Cac8I GCNNGC 3 cut(s) 257, 261, 510
CfoI GCGC 1 cut(s) 242
Cfr10I RCCGGY 1 cut(s) 410
CviAII CATG 1 cut(s) 148
CviJI RGCY 5 cut(s) 24, 106, 202, 308, 508
CviKI_1 RGCY 5 cut(s) 24, 106, 202, 308, 508
DdeI CTNAG 1 cut(s) 524
DpnI GATC 3 cut(s) 277, 424, 464
DpnII GATC 3 cut(s) 275, 422, 462
DraI TTTAAA 1 cut(s) 559
Eam1104I CTCTTC 3 cut(s) 319, 348, 543
EarI CTCTTC 3 cut(s) 319, 348, 543
EciI GGCGGA 3 cut(s) 59, 142, 334
Ecl136II GAGCTC 1 cut(s) 24
Eco24I GRGCYC 1 cut(s) 26
Eco53kI GAGCTC 1 cut(s) 24
Eco57I CTGAAG 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 24
EcoT38I GRGCYC 1 cut(s) 26
Esp3I CGTCTC 1 cut(s) 336
FaeI CATG 1 cut(s) 151
FaiI YATR 1 cut(s) 149
FatI CATG 1 cut(s) 147
FauI CCCGC 5 cut(s) 62, 128, 214, 252, 485
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 3 cut(s) 17, 133, 363
FokI GGATG 2 cut(s) 404, 544
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 3 cut(s) 17, 133, 363
GlaI GCGC 1 cut(s) 241
GluI GCNGC 3 cut(s) 17, 133, 363
GsuI CTGGAG 1 cut(s) 182
HapII CCGG 2 cut(s) 243, 411
HhaI GCGC 1 cut(s) 242
Hin1II CATG 1 cut(s) 151
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HincII GTYRAC 1 cut(s) 185
HindII GTYRAC 1 cut(s) 185
HinfI GANTC 4 cut(s) 302, 336, 458, 535
HpaII CCGG 2 cut(s) 243, 411
HphI GGTGA 2 cut(s) 380, 442
Hpy166II GTNNAC 3 cut(s) 7, 185, 371
Hpy188I TCNGA 5 cut(s) 28, 280, 291, 341, 422
Hpy188III TCNNGA 2 cut(s) 273, 523
Hpy8I GTNNAC 3 cut(s) 7, 185, 371
Hpy99I CGWCG 4 cut(s) 32, 44, 176, 215
HpyAV CCTTC 4 cut(s) 20, 60, 232, 392
HpyCH4IV ACGT 2 cut(s) 30, 373
HpyF10VI GCNNNNNNNGC 2 cut(s) 509, 514
HpyF3I CTNAG 1 cut(s) 524
HpySE526I ACGT 2 cut(s) 30, 373
Hsp92II CATG 1 cut(s) 151
HspAI GCGC 1 cut(s) 240
Kzo9I GATC 3 cut(s) 275, 422, 462
LguI GCTCTTC 1 cut(s) 319
LmnI GCTCC 5 cut(s) 29, 111, 207, 245, 505
LpnPI CCDG 8 cut(s) 212, 256, 351, 374, 424, 494, 536, 560
Lsp1109I GCAGC 1 cut(s) 28
LweI GCATC 1 cut(s) 257
MaeII ACGT 2 cut(s) 30, 373
MalI GATC 3 cut(s) 277, 424, 464
MbiI CCGCTC 3 cut(s) 259, 329, 380
MboI GATC 3 cut(s) 275, 422, 462
MboII GAAGA 7 cut(s) 112, 115, 336, 365, 496, 530, 533
MflI RGATCY 1 cut(s) 275
MhlI GDGCHC 2 cut(s) 26, 265
MluCI AATT 1 cut(s) 575
MlyI GAGTC 1 cut(s) 529
MmeI TCCRAC 3 cut(s) 51, 400, 509
MnlI CCTC 4 cut(s) 83, 97, 200, 246
MroXI GAANNNNTTC 1 cut(s) 437
MseI TTAA 2 cut(s) 558, 585
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 2 cut(s) 243, 411
MvnI CGCG 3 cut(s) 92, 240, 492
MwoI GCNNNNNNNGC 2 cut(s) 509, 514
NdeII GATC 3 cut(s) 275, 422, 462
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 1 cut(s) 68
NspV TTCGAA 1 cut(s) 446
PciSI GCTCTTC 1 cut(s) 319
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 42
PdmI GAANNNNTTC 1 cut(s) 437
PfeI GAWTC 3 cut(s) 302, 336, 458
PflFI GACNNNGTC 1 cut(s) 32
PkrI GCNGC 3 cut(s) 18, 134, 364
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
PshAI GACNNNNGTC 1 cut(s) 180
Psp124BI GAGCTC 1 cut(s) 26
PspN4I GGNNCC 1 cut(s) 68
PsuI RGATCY 1 cut(s) 275
PsyI GACNNNGTC 1 cut(s) 32
SacI GAGCTC 1 cut(s) 26
SapI GCTCTTC 1 cut(s) 319
SaqAI TTAA 2 cut(s) 558, 585
SatI GCNGC 3 cut(s) 17, 133, 363
Sau3AI GATC 3 cut(s) 275, 422, 462
SchI GAGTC 1 cut(s) 529
SduI GDGCHC 2 cut(s) 26, 265
SetI ASST 9 cut(s) 12, 26, 33, 63, 89, 108, 204, 376, 510
SfaNI GCATC 1 cut(s) 257
SfuI TTCGAA 1 cut(s) 446
Sse9I AATT 1 cut(s) 575
SstI GAGCTC 1 cut(s) 26
TaiI ACGT 2 cut(s) 33, 376
TaqI TCGA 3 cut(s) 251, 446, 461
TasI AATT 1 cut(s) 575
TauI GCSGC 2 cut(s) 135, 365
TfiI GAWTC 3 cut(s) 302, 336, 458
Tru1I TTAA 2 cut(s) 558, 585
Tru9I TTAA 2 cut(s) 558, 585
TseI GCWGC 1 cut(s) 16
TspDTI ATGAA 1 cut(s) 136
TspGWI ACGGA 1 cut(s) 399
Tth111I GACNNNGTC 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 6
XmnI GAANNNNTTC 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.