Rmu_sc0000464.1_g000014

Major allergen Pru

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000464.1
N/A
Physical Location & Seq
Forward (+)
57369 .. 58230
862 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 699 bp
atgggtgtgttcacttacgaaaccgagtttacatccgtcatcccaccacctagattgttcaaggctttcatccttgatgcggacaatctcatccccaagattgctccacgagcagttaagtgtgctgaaatcatcgaaggagatggaggagtaggaaccatcaagaagatcaccttcggtgaaggcagccagttcggctctgtgacccacaagatcgatgggattgacaaagacaactttgtctacagctacagtttggtcgaaggagatgccttgtccgacaagattgagaagatctcttacgagaccaaattggtggcatcttccgatggaggatccatcatcaagagcaccagtaactaccataaaatatctggtgctttctctgagctagctgatgatagaaagcaaaacccccaaaataagatcttggggaattcacttaaccaagaatgtgaaaaacactacgcaagccaacgtacctcaaagtttcaacgatccaatcgcttgccttcgaacgagaaaacgttctcaaagtattgccgcgagagagaggtattcgtgtgctcttacaatttacggcacatgcgttgtttttacttcggtggtgtgtttgctttggtttgggtcgtattggggctcagcaaattgtcatgggctggtaaagcagttccatttaaaggtaggctgactccatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

232

Amino Acids

25.89

Weight (kDa)

9.09

Isoelectric Point (pI)

29.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 134 1.2e-20 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
AccI GTMKAC 1 cut(s) 243
AccII CGCG 1 cut(s) 546
AciI CCGC 2 cut(s) 80, 544
AclI AACGTT 1 cut(s) 527
AclWI GGATC 3 cut(s) 330, 343, 492
AcsI RAATTY 1 cut(s) 436
AfaI GTAC 1 cut(s) 481
AfiI CCNNNNNNNGG 2 cut(s) 79, 680
AgsI TTSAA 2 cut(s) 61, 494
AluBI AGCT 3 cut(s) 249, 391, 395
AluI AGCT 3 cut(s) 249, 391, 395
Alw21I GWGCWC 2 cut(s) 353, 569
Alw26I GTCTC 1 cut(s) 299
AlwI GGATC 3 cut(s) 330, 343, 492
ApeKI GCWGC 1 cut(s) 186
ApoI RAATTY 1 cut(s) 436
Asp700I GAANNNNTTC 1 cut(s) 527
AsuHPI GGTGA 2 cut(s) 163, 191
AsuII TTCGAA 1 cut(s) 515
AsuNHI GCTAGC 1 cut(s) 391
BamHI GGATCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 642
BarI GAAGNNNNNNTAC 2 cut(s) 284, 316
BauI CACGAG 1 cut(s) 108
Bbv12I GWGCWC 2 cut(s) 353, 569
BbvI GCAGC 1 cut(s) 198
BccI CCATC 5 cut(s) 137, 167, 212, 323, 347
BceAI ACGGC 1 cut(s) 596
BcgI CGANNNNNNTGC 4 cut(s) 175, 209, 251, 285
BcoDI GTCTC 1 cut(s) 299
BfaI CTAG 2 cut(s) 51, 392
BfmI CTRYAG 2 cut(s) 244, 250
BglI GCCNNNNNGGC 1 cut(s) 195
BglII AGATCT 2 cut(s) 294, 426
BisI GCNGC 2 cut(s) 187, 544
BlpI GCTNAGC 1 cut(s) 641
BlsI GCNGC 2 cut(s) 188, 545
BmiI GGNNCC 2 cut(s) 157, 337
BmsI GCATC 3 cut(s) 67, 259, 329
BmtI GCTAGC 1 cut(s) 395
BplI GAGNNNNNCTC 2 cut(s) 281, 313
Bpu1102I GCTNAGC 1 cut(s) 641
Bpu14I TTCGAA 1 cut(s) 515
Bsa29I ATCGAT 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 299
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 680
Bse1I ACTGG 2 cut(s) 190, 354
BseCI ATCGAT 1 cut(s) 216
BseGI GGATG 4 cut(s) 32, 39, 69, 90
BseLI CCNNNNNNNGG 2 cut(s) 79, 680
BseMII CTCAG 2 cut(s) 378, 655
BseNI ACTGG 2 cut(s) 190, 354
BseRI GAGGAG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 198
Bsh1236I CGCG 1 cut(s) 546
BshVI ATCGAT 1 cut(s) 216
BsiHKAI GWGCWC 2 cut(s) 353, 569
BslI CCNNNNNNNGG 2 cut(s) 79, 680
BsmAI GTCTC 1 cut(s) 299
Bso31I GGTCTC 1 cut(s) 299
Bsp119I TTCGAA 1 cut(s) 515
Bsp1286I GDGCHC 3 cut(s) 353, 569, 642
Bsp143I GATC 6 cut(s) 168, 213, 294, 335, 426, 497
Bsp1720I GCTNAGC 1 cut(s) 641
BspACI CCGC 2 cut(s) 80, 544
BspCNI CTCAG 2 cut(s) 379, 654
BspDI ATCGAT 1 cut(s) 216
BspFNI CGCG 1 cut(s) 546
BspLI GGNNCC 2 cut(s) 157, 337
BspOI GCTAGC 1 cut(s) 395
BspPI GGATC 3 cut(s) 330, 343, 492
BspT104I TTCGAA 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 299
BsrI ACTGG 2 cut(s) 190, 354
BssMI GATC 6 cut(s) 168, 213, 294, 335, 426, 497
BssSI CACGAG 1 cut(s) 108
Bst2BI CACGAG 1 cut(s) 108
Bst4CI ACNGT 1 cut(s) 254
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 3 cut(s) 393, 472, 509
BstDEI CTNAG 2 cut(s) 387, 641
BstF5I GGATG 4 cut(s) 32, 39, 69, 90
BstFNI CGCG 1 cut(s) 546
BstKTI GATC 6 cut(s) 171, 216, 297, 338, 429, 500
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 6 cut(s) 168, 213, 294, 335, 426, 497
BstMWI GCNNNNNNNGC 3 cut(s) 110, 195, 665
BstNSI RCATGY 1 cut(s) 589
BstSFI CTRYAG 2 cut(s) 244, 250
BstUI CGCG 1 cut(s) 546
BstV1I GCAGC 1 cut(s) 198
BstX2I RGATCY 3 cut(s) 294, 335, 426
BstXI CCANNNNNNTGG 1 cut(s) 316
BstYI RGATCY 3 cut(s) 294, 335, 426
Bsu15I ATCGAT 1 cut(s) 216
BsuTUI ATCGAT 1 cut(s) 216
BtsCI GGATG 4 cut(s) 32, 39, 69, 90
Cac8I GCNNGC 3 cut(s) 393, 472, 509
ClaI ATCGAT 1 cut(s) 216
Csp6I GTAC 1 cut(s) 480
CspCI CAANNNNNGTGG 2 cut(s) 36, 71
CviAII CATG 3 cut(s) 586, 654, 696
CviQI GTAC 1 cut(s) 480
DdeI CTNAG 2 cut(s) 387, 641
DpnI GATC 6 cut(s) 170, 215, 296, 337, 428, 499
DpnII GATC 6 cut(s) 168, 213, 294, 335, 426, 497
DraI TTTAAA 1 cut(s) 679
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
Eco24I GRGCYC 1 cut(s) 642
Eco31I GGTCTC 1 cut(s) 299
EcoRI GAATTC 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 642
FaeI CATG 3 cut(s) 589, 657, 699
FaiI YATR 4 cut(s) 366, 587, 655, 697
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FatI CATG 3 cut(s) 585, 653, 695
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 2 cut(s) 187, 544
FokI GGATG 4 cut(s) 19, 26, 56, 77
FriOI GRGCYC 1 cut(s) 642
Fsp4HI GCNGC 2 cut(s) 187, 544
FspBI CTAG 2 cut(s) 51, 392
GluI GCNGC 2 cut(s) 187, 544
Hin1II CATG 3 cut(s) 589, 657, 699
HinfI GANTC 1 cut(s) 691
HphI GGTGA 2 cut(s) 163, 191
Hpy166II GTNNAC 3 cut(s) 12, 30, 244
Hpy188I TCNGA 3 cut(s) 280, 328, 388
Hpy188III TCNNGA 2 cut(s) 163, 346
Hpy8I GTNNAC 3 cut(s) 12, 30, 244
HpyAV CCTTC 5 cut(s) 131, 176, 184, 257, 522
HpyCH4III ACNGT 1 cut(s) 254
HpyCH4IV ACGT 2 cut(s) 478, 527
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 195, 665
HpyF3I CTNAG 2 cut(s) 387, 641
HpySE526I ACGT 2 cut(s) 478, 527
Hsp92II CATG 3 cut(s) 589, 657, 699
Kzo9I GATC 6 cut(s) 168, 213, 294, 335, 426, 497
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 4 cut(s) 203, 360, 367, 645
Lsp1109I GCAGC 1 cut(s) 198
LweI GCATC 3 cut(s) 67, 259, 329
MaeI CTAG 2 cut(s) 51, 392
MaeII ACGT 2 cut(s) 478, 527
MaeIII GTNAC 2 cut(s) 202, 356
MalI GATC 6 cut(s) 170, 215, 296, 337, 428, 499
MboI GATC 6 cut(s) 168, 213, 294, 335, 426, 497
MboII GAAGA 3 cut(s) 178, 304, 315
MflI RGATCY 3 cut(s) 294, 335, 426
MhlI GDGCHC 3 cut(s) 353, 569, 642
MluCI AATT 4 cut(s) 311, 436, 574, 647
MlyI GAGTC 1 cut(s) 685
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 4 cut(s) 140, 326, 493, 547
MroXI GAANNNNTTC 1 cut(s) 527
MseI TTAA 3 cut(s) 117, 444, 678
MvnI CGCG 1 cut(s) 546
MwoI GCNNNNNNNGC 3 cut(s) 110, 195, 665
NdeII GATC 6 cut(s) 168, 213, 294, 335, 426, 497
NheI GCTAGC 1 cut(s) 391
NlaIII CATG 3 cut(s) 589, 657, 699
NlaIV GGNNCC 2 cut(s) 157, 337
NmuCI GTSAC 1 cut(s) 202
NspI RCATGY 1 cut(s) 589
NspV TTCGAA 1 cut(s) 515
PcsI WCGNNNNNNNCGW 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 527
PkrI GCNGC 2 cut(s) 188, 545
PleI GAGTC 1 cut(s) 685
PpsI GAGTC 1 cut(s) 685
Psp1406I AACGTT 1 cut(s) 527
PspN4I GGNNCC 2 cut(s) 157, 337
PsuI RGATCY 3 cut(s) 294, 335, 426
RsaI GTAC 1 cut(s) 481
RsaNI GTAC 1 cut(s) 480
SaqAI TTAA 3 cut(s) 117, 444, 678
SatI GCNGC 2 cut(s) 187, 544
Sau3AI GATC 6 cut(s) 168, 213, 294, 335, 426, 497
SchI GAGTC 1 cut(s) 685
SduI GDGCHC 3 cut(s) 353, 569, 642
SfaNI GCATC 3 cut(s) 67, 259, 329
SfcI CTRYAG 2 cut(s) 244, 250
SfuI TTCGAA 1 cut(s) 515
Sse9I AATT 4 cut(s) 311, 436, 574, 647
SsiI CCGC 2 cut(s) 80, 544
SspMI CTAG 2 cut(s) 51, 392
TaaI ACNGT 1 cut(s) 254
TaiI ACGT 2 cut(s) 481, 530
TaqI TCGA 4 cut(s) 135, 216, 261, 515
TasI AATT 4 cut(s) 311, 436, 574, 647
TauI GCSGC 1 cut(s) 546
Tru1I TTAA 3 cut(s) 117, 444, 678
Tru9I TTAA 3 cut(s) 117, 444, 678
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 1 cut(s) 186
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 1 cut(s) 58
TspGWI ACGGA 1 cut(s) 25
XapI RAATTY 1 cut(s) 436
XceI RCATGY 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 2 cut(s) 215, 371
XmiI GTMKAC 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 527
XspI CTAG 2 cut(s) 51, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.