Rmu_sc0000533.1_g000041

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000533.1
N/A
Physical Location & Seq
Reverse (-)
126393 .. 130044
3652 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1254 bp
atgaggaggagacctggtattggtggtttgcagaatgctgctgctgctagggaccaatatcgggcgctgggccagaatgtggcccagattagaactgatctcatgaaggaacagcttgccacttttaaaagccaattagaagattttgctcgcaaacacaagaatgacattcgcaagaatcctgcttttagatcacaatttcatgatatgtgcactaaggttggagtagaccccttggcctccaataagggtttctgggcagagcttttggggattggtgacttctactatgaacttggagtgcaaattgttgatatctgcttagcaacaagaccccacaatggaggcttgatcaacctgcaggaactctgcaatttgcttcgtcggagacgaaaaaatgatcgtgaagctgtttctgacgatgattgcctgcgtgctataagtaagctgaaggtattgggtaatggttttgaggttatttctgttggaaagaggaagcttgttcgttctgttccaactgagttgaacaaagaccataatgaaattttagagctcgcgcagtctcaaggctttgtgactgttgatgaggtagaaagacggctatcttggaccagtggccgtgcaattgatgccttggacactttactagatgagggccttgcaatgattgatgatggccacaaagatggtatacgccgatattggtttccctgtcaggcggcgaggggctggccggagggaggcccaaggcttctcagacctcttctgggcggcgttcgaggtcggaggaggttaggcttgccgttttctgggcagccacctcacttgcagttgcaggtttggtgtagcacagcagaaccgtcgccggcgactccatcggaccacagacccctccgcacgacccccaacatcccttcggcaagccgcgctgcgccgtcgccactcgatcgaggctggaggagtgacgtccgcactttttctggcctctgcgcacacatcccaccaccgccaagcccttgctacccaacctacagcccttacttccccgcttgcagcttagttgcacagcccccccttccccacgcgcatcgcgccccccccgccctagcccccgcttgcatcacagccctagccccgagcaccttgcagcgtccccgtgcgccctgcagcaacccacctacctatagcagccgagccgagcccagcatcctcgcagcctgcggcgtccggctctggcccagaagaaggacataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
EAP30

Protein Analysis

417

Amino Acids

46.22

Weight (kDa)

9.73

Isoelectric Point (pI)

67.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAP30 PF04157 6 - 225 1.7e-56 EAP30/Vps36 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 969
Acc16I TGCGCA 1 cut(s) 991
Acc36I ACCTGC 2 cut(s) 366, 826
AccB7I CCANNNNNTGG 1 cut(s) 79
AccI GTMKAC 2 cut(s) 228, 691
AccII CGCG 4 cut(s) 557, 927, 1085, 1092
AcoI YGGCCR 3 cut(s) 616, 676, 731
AcsI RAATTY 1 cut(s) 543
AcuI CTGAAG 1 cut(s) 470
AcyI GRCGYC 2 cut(s) 966, 1224
AfiI CCNNNNNNNGG 8 cut(s) 20, 61, 79, 341, 740, 767, 809, 1245
AgsI TTSAA 1 cut(s) 526
AjnI CCWGG 1 cut(s) 13
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 7 cut(s) 115, 265, 410, 448, 499, 553, 1056
AluI AGCT 7 cut(s) 115, 265, 410, 448, 499, 553, 1056
Alw21I GWGCWC 3 cut(s) 215, 555, 1142
Alw26I GTCTC 3 cut(s) 4, 382, 567
Alw44I GTGCAC 1 cut(s) 211
Ama87I CYCGRG 1 cut(s) 1135
ApaLI GTGCAC 1 cut(s) 211
ApoI RAATTY 1 cut(s) 543
AspLEI GCGC 8 cut(s) 67, 559, 929, 934, 992, 1087, 1094, 1162
AspS9I GGNCC 8 cut(s) 52, 70, 82, 609, 655, 743, 880, 1236
AsuHPI GGTGA 1 cut(s) 290
AvaI CYCGRG 1 cut(s) 1135
AvaII GGWCC 3 cut(s) 52, 609, 880
BaeGI GKGCMC 1 cut(s) 215
BalI TGGCCA 1 cut(s) 678
BanII GRGCYC 2 cut(s) 555, 1203
Bbv12I GWGCWC 3 cut(s) 215, 555, 1142
BccI CCATC 3 cut(s) 668, 680, 883
BceAI ACGGC 4 cut(s) 603, 614, 787, 919
BcgI CGANNNNNNTGC 4 cut(s) 843, 877, 1126, 1160
BciT130I CCWGG 1 cut(s) 15
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 3 cut(s) 4, 382, 567
BfaI CTAG 4 cut(s) 48, 647, 1106, 1130
BfmI CTRYAG 4 cut(s) 359, 1030, 1165, 1183
BfoI RGCGCY 1 cut(s) 68
BfuAI ACCTGC 2 cut(s) 366, 826
BlpI GCTNAGC 1 cut(s) 322
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 3 cut(s) 52, 609, 880
BmeT110I CYCGRG 1 cut(s) 1135
BmgT120I GGNCC 8 cut(s) 52, 70, 82, 609, 655, 743, 880, 1236
BmiI GGNNCC 1 cut(s) 53
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 4 cut(s) 619, 1096, 1128, 1215
BpmI CTGGAG 1 cut(s) 976
Bpu1102I GCTNAGC 1 cut(s) 322
BpuEI CTTGAG 1 cut(s) 549
BsaHI GRCGYC 2 cut(s) 966, 1224
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 3 cut(s) 234, 633, 746
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 8 cut(s) 20, 61, 79, 341, 740, 767, 809, 1245
Bse118I RCCGGY 1 cut(s) 865
Bse1I ACTGG 1 cut(s) 612
Bse3DI GCAATG 1 cut(s) 669
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 3 cut(s) 234, 633, 746
BseGI GGATG 3 cut(s) 909, 996, 1206
BseLI CCNNNNNNNGG 8 cut(s) 20, 61, 79, 341, 740, 767, 809, 1245
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 2 cut(s) 510, 769
BseNI ACTGG 1 cut(s) 612
BseRI GAGGAG 4 cut(s) 19, 22, 802, 973
BseSI GKGCMC 1 cut(s) 215
BseYI CCCAGC 2 cut(s) 67, 1202
Bsh1236I CGCG 4 cut(s) 557, 927, 1085, 1092
Bsh1285I CGRYCG 1 cut(s) 949
BsiEI CGRYCG 1 cut(s) 949
BsiHKAI GWGCWC 3 cut(s) 215, 555, 1142
BsiHKCI CYCGRG 1 cut(s) 1135
BsiSI CCGG 3 cut(s) 734, 866, 1228
BslFI GGGAC 2 cut(s) 65, 1137
BslI CCNNNNNNNGG 8 cut(s) 20, 61, 79, 341, 740, 767, 809, 1245
BsmAI GTCTC 3 cut(s) 4, 382, 567
BsmBI CGTCTC 1 cut(s) 382
BsmFI GGGAC 2 cut(s) 65, 1137
BsmI GAATGC 1 cut(s) 40
Bso31I GGTCTC 1 cut(s) 4
BsoBI CYCGRG 1 cut(s) 1135
Bsp1286I GDGCHC 4 cut(s) 215, 555, 1142, 1203
Bsp143I GATC 5 cut(s) 97, 191, 351, 400, 946
Bsp1720I GCTNAGC 1 cut(s) 322
BspCNI CTCAG 2 cut(s) 511, 768
BspFNI CGCG 4 cut(s) 557, 927, 1085, 1092
BspHI TCATGA 2 cut(s) 102, 202
BspLI GGNNCC 1 cut(s) 53
BspMAI CTGCAG 2 cut(s) 363, 1169
BspMI ACCTGC 2 cut(s) 366, 826
BspTNI GGTCTC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 669
BsrFI RCCGGY 1 cut(s) 865
BsrI ACTGG 1 cut(s) 612
BssAI RCCGGY 1 cut(s) 865
BssECI CCNNGG 3 cut(s) 234, 633, 746
BssMI GATC 5 cut(s) 97, 191, 351, 400, 946
BssNAI GTATAC 1 cut(s) 692
BssNI GRCGYC 2 cut(s) 966, 1224
BssT1I CCWWGG 3 cut(s) 234, 633, 746
Bst1107I GTATAC 1 cut(s) 692
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 2 cut(s) 580, 861
Bst6I CTCTTC 1 cut(s) 768
BstACI GRCGYC 2 cut(s) 966, 1224
BstAPI GCANNNNNTGC 1 cut(s) 629
BstDEI CTNAG 5 cut(s) 216, 322, 519, 755, 1057
BstF5I GGATG 3 cut(s) 909, 996, 1206
BstFNI CGCG 4 cut(s) 557, 927, 1085, 1092
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 8 cut(s) 67, 559, 929, 934, 992, 1087, 1094, 1162
BstKTI GATC 5 cut(s) 100, 194, 354, 403, 949
BstMAI GTCTC 3 cut(s) 4, 382, 567
BstMBI GATC 5 cut(s) 97, 191, 351, 400, 946
BstMCI CGRYCG 1 cut(s) 949
BstMWI GCNNNNNNNGC 5 cut(s) 44, 629, 926, 1091, 1100
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 4 cut(s) 359, 1030, 1165, 1183
BstSLI GKGCMC 1 cut(s) 215
BstUI CGCG 4 cut(s) 557, 927, 1085, 1092
BstXI CCANNNNNNTGG 1 cut(s) 686
BstZ17I GTATAC 1 cut(s) 692
BtgZI GCGATG 1 cut(s) 1073
BtsCI GGATG 3 cut(s) 909, 996, 1206
BtsIMutI CAGTG 1 cut(s) 619
BveI ACCTGC 2 cut(s) 366, 826
CciI TCATGA 2 cut(s) 102, 202
CfoI GCGC 8 cut(s) 67, 559, 929, 934, 992, 1087, 1094, 1162
Cfr10I RCCGGY 1 cut(s) 865
Cfr13I GGNCC 8 cut(s) 52, 70, 82, 609, 655, 743, 880, 1236
CseI GACGC 2 cut(s) 1139, 1213
CsiI ACCWGGT 1 cut(s) 13
CspCI CAANNNNNGTGG 2 cut(s) 807, 842
CviAII CATG 2 cut(s) 103, 203
DdeI CTNAG 5 cut(s) 216, 322, 519, 755, 1057
DpnI GATC 5 cut(s) 99, 193, 353, 402, 948
DpnII GATC 5 cut(s) 97, 191, 351, 400, 946
DraI TTTAAA 1 cut(s) 127
EaeI YGGCCR 3 cut(s) 616, 676, 731
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Ecl136II GAGCTC 1 cut(s) 553
Eco130I CCWWGG 3 cut(s) 234, 633, 746
Eco24I GRGCYC 2 cut(s) 555, 1203
Eco31I GGTCTC 1 cut(s) 4
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 3 cut(s) 52, 609, 880
Eco53kI GAGCTC 1 cut(s) 553
Eco57I CTGAAG 1 cut(s) 470
Eco88I CYCGRG 1 cut(s) 1135
EcoICRI GAGCTC 1 cut(s) 553
EcoO109I RGGNCCY 1 cut(s) 655
EcoRII CCWGG 1 cut(s) 13
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 3 cut(s) 234, 633, 746
EcoT38I GRGCYC 2 cut(s) 555, 1203
ErhI CCWWGG 3 cut(s) 234, 633, 746
Esp3I CGTCTC 1 cut(s) 382
FaeI CATG 2 cut(s) 106, 206
FaiI YATR 9 cut(s) 104, 204, 209, 291, 440, 537, 692, 1185, 1252
FaqI GGGAC 2 cut(s) 65, 1137
FatI CATG 2 cut(s) 102, 202
FauI CCCGC 3 cut(s) 1054, 1108, 1120
FbaI TGATCA 1 cut(s) 351
FblI GTMKAC 2 cut(s) 228, 691
FokI GGATG 3 cut(s) 896, 983, 1193
FriOI GRGCYC 2 cut(s) 555, 1203
FspBI CTAG 4 cut(s) 48, 647, 1106, 1130
FspI TGCGCA 1 cut(s) 991
GlaI GCGC 8 cut(s) 66, 558, 928, 933, 991, 1086, 1093, 1161
GsaI CCCAGC 2 cut(s) 71, 1206
GsuI CTGGAG 1 cut(s) 976
HaeII RGCGCY 1 cut(s) 68
HapII CCGG 3 cut(s) 734, 866, 1228
HgaI GACGC 2 cut(s) 1139, 1213
HhaI GCGC 8 cut(s) 67, 559, 929, 934, 992, 1087, 1094, 1162
Hin1I GRCGYC 2 cut(s) 966, 1224
Hin1II CATG 2 cut(s) 106, 206
Hin6I GCGC 8 cut(s) 65, 557, 927, 932, 990, 1085, 1092, 1160
HinP1I GCGC 8 cut(s) 65, 557, 927, 932, 990, 1085, 1092, 1160
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 2 cut(s) 178, 871
HpaII CCGG 3 cut(s) 734, 866, 1228
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 3 cut(s) 213, 229, 692
Hpy188I TCNGA 5 cut(s) 387, 418, 758, 786, 880
Hpy188III TCNNGA 3 cut(s) 103, 203, 404
Hpy8I GTNNAC 3 cut(s) 213, 229, 692
Hpy99I CGWCG 3 cut(s) 387, 865, 940
HpyAV CCTTC 5 cut(s) 100, 445, 924, 1085, 1239
HpyCH4III ACNGT 2 cut(s) 580, 861
HpyCH4IV ACGT 1 cut(s) 966
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 629, 926, 1091, 1100
HpyF3I CTNAG 5 cut(s) 216, 322, 519, 755, 1057
HpySE526I ACGT 1 cut(s) 966
Hsp92I GRCGYC 2 cut(s) 966, 1224
Hsp92II CATG 2 cut(s) 106, 206
HspAI GCGC 8 cut(s) 65, 557, 927, 932, 990, 1085, 1092, 1160
KroI GCCGGC 1 cut(s) 865
KroNI GCCGGC 1 cut(s) 867
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 5 cut(s) 97, 191, 351, 400, 946
LweI GCATC 4 cut(s) 619, 1096, 1128, 1215
MabI ACCWGGT 1 cut(s) 13
MaeI CTAG 4 cut(s) 48, 647, 1106, 1130
MaeII ACGT 1 cut(s) 966
MaeIII GTNAC 3 cut(s) 278, 574, 962
MalI GATC 5 cut(s) 99, 193, 353, 402, 948
MboI GATC 5 cut(s) 97, 191, 351, 400, 946
MboII GAAGA 3 cut(s) 152, 755, 1254
MfeI CAATTG 1 cut(s) 624
MhlI GDGCHC 4 cut(s) 215, 555, 1142, 1203
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 6 cut(s) 134, 197, 306, 373, 543, 624
MluNI TGGCCA 1 cut(s) 678
MlyI GAGTC 1 cut(s) 865
MmeI TCCRAC 5 cut(s) 202, 365, 466, 539, 764
Mox20I TGGCCA 1 cut(s) 678
MreI CGCCGGCG 1 cut(s) 865
MroNI GCCGGC 1 cut(s) 865
MscI TGGCCA 1 cut(s) 678
MseI TTAA 1 cut(s) 126
MslI CAYNNNNRTG 2 cut(s) 162, 684
Msp20I TGGCCA 1 cut(s) 678
MspI CCGG 3 cut(s) 734, 866, 1228
MspR9I CCNGG 1 cut(s) 15
MteI GCGCNGCGC 1 cut(s) 930
MunI CAATTG 1 cut(s) 624
Mva1269I GAATGC 1 cut(s) 40
MvaI CCWGG 1 cut(s) 15
MvnI CGCG 4 cut(s) 557, 927, 1085, 1092
MwoI GCNNNNNNNGC 5 cut(s) 44, 629, 926, 1091, 1100
NaeI GCCGGC 1 cut(s) 867
NdeII GATC 5 cut(s) 97, 191, 351, 400, 946
NgoMIV GCCGGC 1 cut(s) 865
NlaIII CATG 2 cut(s) 106, 206
NlaIV GGNNCC 1 cut(s) 53
NmeAIII GCCGAG 2 cut(s) 1217, 1222
NmuCI GTSAC 3 cut(s) 278, 574, 962
NsbI TGCGCA 1 cut(s) 991
PagI TCATGA 2 cut(s) 102, 202
PcsI WCGNNNNNNNCGW 1 cut(s) 388
PctI GAATGC 1 cut(s) 40
PdiI GCCGGC 1 cut(s) 867
PfeI GAWTC 1 cut(s) 178
PflMI CCANNNNNTGG 1 cut(s) 79
Ple19I CGATCG 1 cut(s) 949
PleI GAGTC 1 cut(s) 865
PpsI GAGTC 1 cut(s) 865
Psp124BI GAGCTC 1 cut(s) 555
Psp6I CCWGG 1 cut(s) 13
PspFI CCCAGC 2 cut(s) 67, 1202
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 53
PspPI GGNCC 8 cut(s) 52, 70, 82, 609, 655, 743, 880, 1236
PstI CTGCAG 2 cut(s) 363, 1169
PvuI CGATCG 1 cut(s) 949
RseI CAYNNNNRTG 2 cut(s) 162, 684
SacI GAGCTC 1 cut(s) 555
SaqAI TTAA 1 cut(s) 126
Sau3AI GATC 5 cut(s) 97, 191, 351, 400, 946
Sau96I GGNCC 8 cut(s) 52, 70, 82, 609, 655, 743, 880, 1236
SbfI CCTGCAGG 1 cut(s) 363
SchI GAGTC 1 cut(s) 865
ScrFI CCNGG 1 cut(s) 15
SdaI CCTGCAGG 1 cut(s) 363
SduI GDGCHC 4 cut(s) 215, 555, 1142, 1203
SexAI ACCWGGT 1 cut(s) 13
SfaNI GCATC 4 cut(s) 619, 1096, 1128, 1215
SfcI CTRYAG 4 cut(s) 359, 1030, 1165, 1183
SgrAI CRCCGGYG 1 cut(s) 865
SinI GGWCC 3 cut(s) 52, 609, 880
SmiMI CAYNNNNRTG 2 cut(s) 162, 684
SmlI CTYRAG 1 cut(s) 564
SmoI CTYRAG 1 cut(s) 564
Sse8387I CCTGCAGG 1 cut(s) 363
Sse9I AATT 6 cut(s) 134, 197, 306, 373, 543, 624
SspMI CTAG 4 cut(s) 48, 647, 1106, 1130
SstI GAGCTC 1 cut(s) 555
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 3 cut(s) 234, 633, 746
TaaI ACNGT 2 cut(s) 580, 861
TaiI ACGT 1 cut(s) 969
TaqI TCGA 3 cut(s) 778, 945, 949
TasI AATT 6 cut(s) 134, 197, 306, 373, 543, 624
TauI GCSGC 4 cut(s) 722, 774, 927, 1224
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TscAI CASTG 1 cut(s) 619
TseFI GTSAC 3 cut(s) 278, 574, 962
Tsp45I GTSAC 3 cut(s) 278, 574, 962
TspDTI ATGAA 4 cut(s) 119, 191, 306, 555
TspRI CASTG 1 cut(s) 619
Van91I CCANNNNNTGG 1 cut(s) 79
VneI GTGCAC 1 cut(s) 211
VpaK11BI GGWCC 3 cut(s) 52, 609, 880
XapI RAATTY 1 cut(s) 543
XmiI GTMKAC 2 cut(s) 228, 691
XspI CTAG 4 cut(s) 48, 647, 1106, 1130
ZraI GACGTC 1 cut(s) 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.