Rmu_sc0000538.1_g000001

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000538.1
N/A
Physical Location & Seq
Forward (+)
1 .. 704
704 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 464 bp
tatctgagttggtagccgtgcaaagtgagctttggaatacaaggcaaggtcctcatcttatgaggaaactagatgttgatgtatttgctgctagacctgagcagtggagggccagacaagaatcaaagcaatcaactttgagagacttttctgctacatttggctctagtgagaccaaaccatcaaacaatagtgagactgagcaatcaaagaagagcagctttctttctgatgattataagaaaaaatcaaaagtgggaaaaacagttgaccatcaccaggcatctcctgcaccatttttgagtacaacaggcttcaaacgtaagcccaaggaaaaagaacagagcaacaagaagtttgcaagagtctcggtagatgaggatgtttctaaagtgaagaataagtcaaagaagaaaaggcatggtgacgatgctttttctaaatcccccaagttgaaagcatga

Protein Analysis

153

Amino Acids

17.36

Weight (kDa)

9.99

Isoelectric Point (pI)

56.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 239
AfaI GTAC 1 cut(s) 306
AfiI CCNNNNNNNGG 1 cut(s) 279
AgsI TTSAA 2 cut(s) 318, 456
AjnI CCWGG 1 cut(s) 278
AluBI AGCT 2 cut(s) 30, 221
AluI AGCT 2 cut(s) 30, 221
Alw26I GTCTC 4 cut(s) 137, 166, 190, 372
AoxI GGCC 1 cut(s) 110
ApeKI GCWGC 2 cut(s) 88, 218
AspS9I GGNCC 2 cut(s) 49, 110
AsuHPI GGTGA 2 cut(s) 268, 436
AvaII GGWCC 1 cut(s) 49
BbvI GCAGC 2 cut(s) 75, 230
BccI CCATC 2 cut(s) 189, 281
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BciT130I CCWGG 1 cut(s) 280
BcoDI GTCTC 4 cut(s) 137, 166, 190, 372
BfaI CTAG 3 cut(s) 70, 92, 167
BisI GCNGC 2 cut(s) 89, 219
BlsI GCNGC 2 cut(s) 90, 220
Bme1390I CCNGG 1 cut(s) 280
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 2 cut(s) 49, 110
BmrFI CCNGG 1 cut(s) 280
BmsI GCATC 2 cut(s) 292, 420
Bpu10I CCTNAGC 1 cut(s) 98
BsaI GGTCTC 1 cut(s) 166
BsaJI CCNNGG 1 cut(s) 329
Bsc4I CCNNNNNNNGG 1 cut(s) 279
BseBI CCWGG 1 cut(s) 280
BseDI CCNNGG 1 cut(s) 329
BseGI GGATG 1 cut(s) 387
BseLI CCNNNNNNNGG 1 cut(s) 279
BseMII CTCAG 2 cut(s) 89, 191
BseXI GCAGC 2 cut(s) 75, 230
BsgI GTGCAG 1 cut(s) 275
BshFI GGCC 1 cut(s) 112
BslI CCNNNNNNNGG 1 cut(s) 279
BsmAI GTCTC 4 cut(s) 137, 166, 190, 372
BsnI GGCC 1 cut(s) 112
Bso31I GGTCTC 1 cut(s) 166
BspANI GGCC 1 cut(s) 112
BspCNI CTCAG 2 cut(s) 90, 192
BspQI GCTCTTC 1 cut(s) 208
BspTNI GGTCTC 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 329
BssT1I CCWWGG 1 cut(s) 329
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 1 cut(s) 267
Bst6I CTCTTC 1 cut(s) 208
BstAPI GCANNNNNTGC 1 cut(s) 289
BstDEI CTNAG 3 cut(s) 5, 98, 200
BstF5I GGATG 1 cut(s) 387
BstMAI GTCTC 4 cut(s) 137, 166, 190, 372
BstMWI GCNNNNNNNGC 2 cut(s) 27, 289
BstNI CCWGG 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 278
BstV1I GCAGC 2 cut(s) 75, 230
BsuRI GGCC 1 cut(s) 112
BtsCI GGATG 1 cut(s) 387
BtsI GCAGTG 1 cut(s) 109
BtsIMutI CAGTG 1 cut(s) 109
Cfr13I GGNCC 2 cut(s) 49, 110
Csp6I GTAC 1 cut(s) 305
CviAII CATG 2 cut(s) 421, 461
CviJI RGCY 7 cut(s) 16, 30, 112, 164, 221, 314, 327
CviKI_1 RGCY 7 cut(s) 16, 30, 112, 164, 221, 314, 327
CviQI GTAC 1 cut(s) 305
DdeI CTNAG 3 cut(s) 5, 98, 200
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 329
Eco31I GGTCTC 1 cut(s) 166
Eco47I GGWCC 1 cut(s) 49
EcoO109I RGGNCCY 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 278
EcoT14I CCWWGG 1 cut(s) 329
ErhI CCWWGG 1 cut(s) 329
FaeI CATG 2 cut(s) 424, 464
FaiI YATR 4 cut(s) 61, 239, 422, 462
FalI AAGNNNNNCTT 2 cut(s) 205, 237
FatI CATG 2 cut(s) 420, 460
Fnu4HI GCNGC 2 cut(s) 89, 219
FokI GGATG 1 cut(s) 394
Fsp4HI GCNGC 2 cut(s) 89, 219
FspBI CTAG 3 cut(s) 70, 92, 167
GluI GCNGC 2 cut(s) 89, 219
HaeIII GGCC 1 cut(s) 112
Hin1II CATG 2 cut(s) 424, 464
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HinfI GANTC 2 cut(s) 121, 365
HphI GGTGA 2 cut(s) 268, 436
Hpy166II GTNNAC 1 cut(s) 270
Hpy188I TCNGA 2 cut(s) 6, 231
Hpy8I GTNNAC 1 cut(s) 270
HpyCH4III ACNGT 1 cut(s) 267
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 3 cut(s) 21, 292, 361
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 289
HpyF3I CTNAG 3 cut(s) 5, 98, 200
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 2 cut(s) 424, 464
LguI GCTCTTC 1 cut(s) 208
LpnPI CCDG 6 cut(s) 110, 126, 265, 292, 296, 302
Lsp1109I GCAGC 2 cut(s) 75, 230
LweI GCATC 2 cut(s) 292, 420
MaeI CTAG 3 cut(s) 70, 92, 167
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 424
MboII GAAGA 3 cut(s) 225, 408, 423
MlyI GAGTC 1 cut(s) 374
MnlI CCTC 4 cut(s) 56, 62, 101, 372
MspR9I CCNGG 1 cut(s) 280
MvaI CCWGG 1 cut(s) 280
MwoI GCNNNNNNNGC 2 cut(s) 27, 289
NlaIII CATG 2 cut(s) 424, 464
NmuCI GTSAC 1 cut(s) 424
PciSI GCTCTTC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 121
PkrI GCNGC 2 cut(s) 90, 220
PleI GAGTC 1 cut(s) 373
PpsI GAGTC 1 cut(s) 373
PpuMI RGGWCCY 1 cut(s) 49
PsiI TTATAA 1 cut(s) 239
Psp5II RGGWCCY 1 cut(s) 49
Psp6I CCWGG 1 cut(s) 278
PspGI CCWGG 1 cut(s) 278
PspPI GGNCC 2 cut(s) 49, 110
PspPPI RGGWCCY 1 cut(s) 49
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
SapI GCTCTTC 1 cut(s) 208
SatI GCNGC 2 cut(s) 89, 219
Sau96I GGNCC 2 cut(s) 49, 110
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 1 cut(s) 280
SetI ASST 5 cut(s) 32, 51, 99, 223, 324
SfaNI GCATC 2 cut(s) 292, 420
SinI GGWCC 1 cut(s) 49
SspMI CTAG 3 cut(s) 70, 92, 167
StyD4I CCNGG 1 cut(s) 278
StyI CCWWGG 1 cut(s) 329
TaaI ACNGT 1 cut(s) 267
TaiI ACGT 1 cut(s) 324
TatI WGTACW 1 cut(s) 304
TfiI GAWTC 1 cut(s) 121
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 2 cut(s) 88, 218
Tsp45I GTSAC 1 cut(s) 424
TspRI CASTG 1 cut(s) 109
VpaK11BI GGWCC 1 cut(s) 49
XspI CTAG 3 cut(s) 70, 92, 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.