Rmu_sc0000639.1_g000008

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000639.1
N/A
Physical Location & Seq
Reverse (-)
31286 .. 32403
1118 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 606 bp
atgagttggatggaagcctggagctcttttgactaccgtacactaacccaagtagcctttgatcattgccccctgctattgagtttcaaccaacttcctgtggtctttaaatcccaattcaggtttcaacgtatgtggctgcaactctcagatttcttccctctagtaagtgatttctggtcaggtcttcagtgtgggggttgccctatgtttgtattatccatgaagcttcgagctctagaagtaaggctaaaggaatggaatgcaggctcttttggtaatgttgatgaactggtaaacactgcggagaccgagcttgacttaattcaatcggaaatctcaaatttggccttcaccaaggatagtaacatcacggactcggggctagtggaaagagtcattccgagtttggtaacgtcttcagagaattttggtctcactaccattcctactcctgaggaagttcatgcagcagtcaactccatggatgagtatagtttgccggggccggatgggagcttcagcaatgaaatgcagaaatgtagaagcaagatcttctgcactgctcactcagtatttagtactctctatggggcgagattctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

201

Amino Acids

22.7

Weight (kDa)

4.74

Isoelectric Point (pI)

34.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 305
AcsI RAATTY 2 cut(s) 343, 427
AcuI CTGAAG 3 cut(s) 173, 405, 505
AfaI GTAC 2 cut(s) 40, 583
AfiI CCNNNNNNNGG 1 cut(s) 120
AgsI TTSAA 3 cut(s) 88, 128, 329
AjnI CCWGG 1 cut(s) 17
AjuI GAANNNNNNNTTGG 2 cut(s) 108, 140
AluBI AGCT 5 cut(s) 24, 229, 236, 316, 519
AluI AGCT 5 cut(s) 24, 229, 236, 316, 519
Alw21I GWGCWC 2 cut(s) 26, 238
Alw26I GTCTC 2 cut(s) 302, 440
Ama87I CYCGRG 1 cut(s) 379
AoxI GGCC 2 cut(s) 348, 506
ApeKI GCWGC 2 cut(s) 139, 470
ApoI RAATTY 2 cut(s) 343, 427
AspS9I GGNCC 1 cut(s) 506
AsuC2I CCSGG 1 cut(s) 504
AsuHPI GGTGA 1 cut(s) 346
AvaI CYCGRG 1 cut(s) 379
AxyI CCTNAGG 1 cut(s) 456
BanII GRGCYC 2 cut(s) 26, 238
BbsI GAAGAC 2 cut(s) 179, 411
Bbv12I GWGCWC 2 cut(s) 26, 238
BbvI GCAGC 2 cut(s) 126, 482
BccI CCATC 2 cut(s) 4, 506
BciT130I CCWGG 1 cut(s) 19
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 1 cut(s) 504
BcoDI GTCTC 2 cut(s) 302, 440
BfaI CTAG 3 cut(s) 164, 239, 386
BglII AGATCT 1 cut(s) 552
BisI GCNGC 2 cut(s) 140, 471
BlsI GCNGC 2 cut(s) 141, 472
BmcAI AGTACT 1 cut(s) 583
Bme1390I CCNGG 2 cut(s) 19, 504
BmeT110I CYCGRG 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 506
BmiI GGNNCC 1 cut(s) 507
BmrFI CCNGG 2 cut(s) 19, 504
BpiI GAAGAC 2 cut(s) 179, 411
BpmI CTGGAG 1 cut(s) 40
BpuMI CCSGG 1 cut(s) 504
BsaI GGTCTC 2 cut(s) 302, 440
BsaJI CCNNGG 3 cut(s) 357, 483, 503
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse1I ACTGG 1 cut(s) 297
Bse21I CCTNAGG 1 cut(s) 456
Bse3DI GCAATG 2 cut(s) 64, 532
BseBI CCWGG 1 cut(s) 19
BseDI CCNNGG 3 cut(s) 357, 483, 503
BseGI GGATG 3 cut(s) 15, 493, 517
BseLI CCNNNNNNNGG 1 cut(s) 120
BseMI GCAATG 2 cut(s) 64, 532
BseMII CTCAG 3 cut(s) 162, 447, 585
BseNI ACTGG 1 cut(s) 297
BseXI GCAGC 2 cut(s) 126, 482
BsgI GTGCAG 1 cut(s) 544
BshFI GGCC 2 cut(s) 350, 508
BsiHKAI GWGCWC 2 cut(s) 26, 238
BsiHKCI CYCGRG 1 cut(s) 379
BsiSI CCGG 2 cut(s) 503, 509
BslI CCNNNNNNNGG 1 cut(s) 120
BsmAI GTCTC 2 cut(s) 302, 440
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 2 cut(s) 350, 508
Bso31I GGTCTC 2 cut(s) 302, 440
BsoBI CYCGRG 1 cut(s) 379
Bsp1286I GDGCHC 2 cut(s) 26, 238
Bsp143I GATC 2 cut(s) 61, 552
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 1 cut(s) 305
BspANI GGCC 2 cut(s) 350, 508
BspCNI CTCAG 3 cut(s) 161, 448, 584
BspLI GGNNCC 1 cut(s) 507
BspTNI GGTCTC 2 cut(s) 302, 440
BsrDI GCAATG 2 cut(s) 64, 532
BsrI ACTGG 1 cut(s) 297
BssECI CCNNGG 3 cut(s) 357, 483, 503
BssMI GATC 2 cut(s) 61, 552
BssT1I CCWWGG 2 cut(s) 357, 483
Bst2UI CCWGG 1 cut(s) 19
Bst4CI ACNGT 1 cut(s) 38
BstC8I GCNNGC 1 cut(s) 268
BstDEI CTNAG 3 cut(s) 148, 456, 571
BstDSI CCRYGG 1 cut(s) 483
BstF5I GGATG 3 cut(s) 15, 493, 517
BstKTI GATC 2 cut(s) 64, 555
BstMAI GTCTC 2 cut(s) 302, 440
BstMBI GATC 2 cut(s) 61, 552
BstNI CCWGG 1 cut(s) 19
BstSCI CCNGG 2 cut(s) 17, 502
BstV1I GCAGC 2 cut(s) 126, 482
BstV2I GAAGAC 2 cut(s) 179, 411
BstX2I RGATCY 1 cut(s) 552
BstYI RGATCY 1 cut(s) 552
Bsu36I CCTNAGG 1 cut(s) 456
BsuRI GGCC 2 cut(s) 350, 508
BtgI CCRYGG 1 cut(s) 483
BtsCI GGATG 3 cut(s) 15, 493, 517
BtsI GCAGTG 2 cut(s) 300, 561
BtsIMutI CAGTG 3 cut(s) 197, 300, 561
Cac8I GCNNGC 1 cut(s) 268
Cfr13I GGNCC 1 cut(s) 506
Csp6I GTAC 2 cut(s) 39, 582
CspCI CAANNNNNGTGG 2 cut(s) 116, 151
CviAII CATG 3 cut(s) 223, 467, 484
CviQI GTAC 2 cut(s) 39, 582
DdeI CTNAG 3 cut(s) 148, 456, 571
DpnI GATC 2 cut(s) 63, 554
DpnII GATC 2 cut(s) 61, 552
DraI TTTAAA 1 cut(s) 109
Ecl136II GAGCTC 2 cut(s) 24, 236
Eco130I CCWWGG 2 cut(s) 357, 483
Eco24I GRGCYC 2 cut(s) 26, 238
Eco31I GGTCTC 2 cut(s) 302, 440
Eco53kI GAGCTC 2 cut(s) 24, 236
Eco57I CTGAAG 3 cut(s) 173, 405, 505
Eco81I CCTNAGG 1 cut(s) 456
Eco88I CYCGRG 1 cut(s) 379
EcoICRI GAGCTC 2 cut(s) 24, 236
EcoRII CCWGG 1 cut(s) 17
EcoT14I CCWWGG 2 cut(s) 357, 483
EcoT38I GRGCYC 2 cut(s) 26, 238
ErhI CCWWGG 2 cut(s) 357, 483
FaeI CATG 3 cut(s) 226, 470, 487
FaiI YATR 7 cut(s) 134, 209, 224, 468, 485, 495, 591
FatI CATG 3 cut(s) 222, 466, 483
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 2 cut(s) 140, 471
FokI GGATG 3 cut(s) 22, 500, 524
FriOI GRGCYC 2 cut(s) 26, 238
Fsp4HI GCNGC 2 cut(s) 140, 471
FspBI CTAG 3 cut(s) 164, 239, 386
GluI GCNGC 2 cut(s) 140, 471
GsuI CTGGAG 1 cut(s) 40
HaeIII GGCC 2 cut(s) 350, 508
HapII CCGG 2 cut(s) 503, 509
Hin1II CATG 3 cut(s) 226, 470, 487
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 3 cut(s) 377, 396, 600
HpaII CCGG 2 cut(s) 503, 509
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 3 cut(s) 41, 298, 478
Hpy188I TCNGA 5 cut(s) 151, 334, 405, 424, 605
Hpy188III TCNNGA 2 cut(s) 239, 455
Hpy8I GTNNAC 3 cut(s) 41, 298, 478
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 1 cut(s) 38
HpyCH4IV ACGT 2 cut(s) 130, 416
HpyCH4V TGCA 5 cut(s) 142, 266, 470, 535, 561
HpyF3I CTNAG 3 cut(s) 148, 456, 571
HpySE526I ACGT 2 cut(s) 130, 416
Hsp92II CATG 3 cut(s) 226, 470, 487
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 2 cut(s) 61, 552
LmnI GCTCC 2 cut(s) 21, 516
Lsp1109I GCAGC 2 cut(s) 126, 482
MaeI CTAG 3 cut(s) 164, 239, 386
MaeII ACGT 2 cut(s) 130, 416
MaeIII GTNAC 2 cut(s) 365, 412
MalI GATC 2 cut(s) 63, 554
MboI GATC 2 cut(s) 61, 552
MboII GAAGA 4 cut(s) 148, 179, 411, 547
MflI RGATCY 1 cut(s) 552
MhlI GDGCHC 2 cut(s) 26, 238
MluCI AATT 4 cut(s) 116, 324, 343, 427
MlyI GAGTC 2 cut(s) 371, 405
MnlI CCTC 2 cut(s) 171, 451
MseI TTAA 2 cut(s) 108, 323
MspI CCGG 2 cut(s) 503, 509
MspR9I CCNGG 2 cut(s) 19, 504
Mva1269I GAATGC 1 cut(s) 268
MvaI CCWGG 1 cut(s) 19
NciI CCSGG 1 cut(s) 504
NcoI CCATGG 1 cut(s) 483
NdeII GATC 2 cut(s) 61, 552
NlaIII CATG 3 cut(s) 226, 470, 487
NlaIV GGNNCC 1 cut(s) 507
PctI GAATGC 1 cut(s) 268
PfeI GAWTC 1 cut(s) 600
PkrI GCNGC 2 cut(s) 141, 472
PleI GAGTC 2 cut(s) 371, 404
PpsI GAGTC 2 cut(s) 371, 404
Psp124BI GAGCTC 2 cut(s) 26, 238
Psp6I CCWGG 1 cut(s) 17
PspGI CCWGG 1 cut(s) 17
PspN4I GGNNCC 1 cut(s) 507
PspPI GGNCC 1 cut(s) 506
PsuI RGATCY 1 cut(s) 552
RsaI GTAC 2 cut(s) 40, 583
RsaNI GTAC 2 cut(s) 39, 582
SacI GAGCTC 2 cut(s) 26, 238
SaqAI TTAA 2 cut(s) 108, 323
SatI GCNGC 2 cut(s) 140, 471
Sau3AI GATC 2 cut(s) 61, 552
Sau96I GGNCC 1 cut(s) 506
ScaI AGTACT 1 cut(s) 583
SchI GAGTC 2 cut(s) 371, 405
ScrFI CCNGG 2 cut(s) 19, 504
SduI GDGCHC 2 cut(s) 26, 238
SetI ASST 9 cut(s) 26, 125, 133, 187, 231, 238, 318, 419, 521
Sse9I AATT 4 cut(s) 116, 324, 343, 427
SsiI CCGC 1 cut(s) 305
SspMI CTAG 3 cut(s) 164, 239, 386
SstI GAGCTC 2 cut(s) 26, 238
StyD4I CCNGG 2 cut(s) 17, 502
StyI CCWWGG 2 cut(s) 357, 483
TaaI ACNGT 1 cut(s) 38
TaiI ACGT 2 cut(s) 133, 419
TaqI TCGA 1 cut(s) 232
TaqII GACCGA 1 cut(s) 326
TasI AATT 4 cut(s) 116, 324, 343, 427
TatI WGTACW 1 cut(s) 581
TfiI GAWTC 1 cut(s) 600
Tru1I TTAA 2 cut(s) 108, 323
Tru9I TTAA 2 cut(s) 108, 323
TscAI CASTG 3 cut(s) 197, 307, 568
TseI GCWGC 2 cut(s) 139, 470
TspDTI ATGAA 4 cut(s) 239, 303, 455, 543
TspGWI ACGGA 1 cut(s) 389
TspRI CASTG 3 cut(s) 197, 307, 568
XapI RAATTY 2 cut(s) 343, 427
XbaI TCTAGA 1 cut(s) 238
XspI CTAG 3 cut(s) 164, 239, 386
ZrmI AGTACT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.