Rmu_sc0000674.1_g000027

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000674.1
N/A
Physical Location & Seq
Forward (+)
139427 .. 141337
1911 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1209 bp
atgtcgcagcctagggcagtagtgcccccctccacaggcgcacagctttggccagctaatatgtgggagcagagtaacggctacattttaaaatcatcgaaatttgatatcctacgtggcggcgctacgtggaggctggactcagatgacgtggactctggctctggagggtcccgactcccgaactcaaattgggctataaaaggtggtgtatttctcgtgtctcagactcagagctctctcacttctttcttctttactaatccctttccttcttcctctaagctcttaagctcttcttcaatggctactacttcagcctcgaaagtcatgtctctgtctctcagtgtgattttgggtctctcattgttcttgggtctggtcagctcggccaaattcgaccagcttttccagccctactgggcctccgaccatttcacatacgaaggcgagcttcttcatatgaagctcgacaatttttccggagctgggttttcatccaagaacaagtacatgtttggcaaagtgaccgtacagattaagctcatagagggtgactctgctggaactgtcactgctttctatatgtcatcggacggtccactgcacaacgagtttgatttcgagttcctgggcaacaccacaggggagccttactctgttcagaccaacctctatatcaatggcgtcggcaatagagagcaaaggcttgacctttggtttgacccaaccacggacttccattcctactccattttctggaaccagcgccaagtagtgtttctagtggatgagaccccaattagggtacacacaaacatggaaagcaagggtttgcccttccccaaggaccaggctatgggggtatacagctcaatctggaatgccgatgactgggccacacagggcgggcgggtcaagaccgattggtcccatggaccctttgttgcatcttataagggctttgacatcaatgcctgtgagtgcccggtctccgtggccggagccgataacgcgaagaagtgcaccagcagcgcagacaagaagtactggtgggatgagcctgtgctgtccgagctgaacgtccaccagaaccaccagctggtgtgggttaagaaccaccacatggtctatgactactgcactgatactgctaggttcccagtcactccagttgagtgtgtgcaccacggtcaccaccgccactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016740 GO:0016757 GO:0016758 GO:0016762 GO:0046527
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

402

Amino Acids

44.88

Weight (kDa)

5.99

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 135 - 311 1.1e-60 Glycosyl hydrolases family 16
XET_C PF06955 347 - 394 1.8e-17 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 957
AasI GACNNNNNNGTC 1 cut(s) 928
AccB7I CCANNNNNTGG 4 cut(s) 759, 859, 1102, 1126
AccI GTMKAC 1 cut(s) 867
AccII CGCG 1 cut(s) 1016
AccIII TCCGGA 1 cut(s) 482
AciI CCGC 4 cut(s) 120, 909, 913, 1201
AcoI YGGCCR 3 cut(s) 50, 390, 999
AcsI RAATTY 2 cut(s) 101, 395
AcuI CTGAAG 1 cut(s) 300
AcyI GRCGYC 1 cut(s) 687
AfaI GTAC 4 cut(s) 512, 534, 810, 1049
AflII CTTAAG 1 cut(s) 289
AflIII ACRYGT 1 cut(s) 513
AgsI TTSAA 1 cut(s) 303
AjiI CACGTC 1 cut(s) 151
AjnI CCWGG 2 cut(s) 630, 852
AjuI GAANNNNNNNTTGG 2 cut(s) 765, 797
Alw21I GWGCWC 3 cut(s) 239, 1028, 1188
Alw26I GTCTC 6 cut(s) 228, 339, 345, 365, 788, 997
Alw44I GTGCAC 2 cut(s) 1024, 1184
Aor13HI TCCGGA 1 cut(s) 482
AoxI GGCC 5 cut(s) 50, 390, 423, 897, 999
ApaLI GTGCAC 2 cut(s) 1024, 1184
ApeKI GCWGC 2 cut(s) 7, 1032
ApoI RAATTY 2 cut(s) 101, 395
ArsI GACNNNNNNTTYG 4 cut(s) 317, 349, 704, 736
AspA2I CCTAGG 1 cut(s) 11
AspLEI GCGC 4 cut(s) 41, 125, 771, 1037
AspS9I GGNCC 7 cut(s) 171, 423, 599, 850, 897, 930, 938
AsuC2I CCSGG 1 cut(s) 989
AsuHPI GGTGA 2 cut(s) 566, 1187
AvaII GGWCC 5 cut(s) 171, 599, 850, 930, 938
AvrII CCTAGG 1 cut(s) 11
BaeGI GKGCMC 4 cut(s) 27, 989, 1028, 1188
BalI TGGCCA 1 cut(s) 52
BanII GRGCYC 1 cut(s) 239
BauI CACGAG 1 cut(s) 218
Bbv12I GWGCWC 3 cut(s) 239, 1028, 1188
BbvI GCAGC 2 cut(s) 19, 1044
BceAI ACGGC 1 cut(s) 94
BciT130I CCWGG 2 cut(s) 632, 854
BcnI CCSGG 1 cut(s) 989
BcoDI GTCTC 6 cut(s) 228, 339, 345, 365, 788, 997
BfaI CTAG 4 cut(s) 12, 785, 1155, 1207
BfoI RGCGCY 2 cut(s) 126, 772
BfrI CTTAAG 1 cut(s) 289
BisI GCNGC 3 cut(s) 8, 121, 1033
BlnI CCTAGG 1 cut(s) 11
BlsI GCNGC 3 cut(s) 9, 122, 1034
BmcAI AGTACT 1 cut(s) 1049
Bme1390I CCNGG 3 cut(s) 632, 854, 989
Bme18I GGWCC 5 cut(s) 171, 599, 850, 930, 938
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 7 cut(s) 171, 423, 599, 850, 897, 930, 938
BmiI GGNNCC 8 cut(s) 172, 173, 651, 764, 932, 940, 1006, 1160
BmrFI CCNGG 3 cut(s) 632, 854, 989
BmrI ACTGGG 3 cut(s) 430, 904, 1157
BmsI GCATC 1 cut(s) 959
BmuI ACTGGG 3 cut(s) 430, 904, 1157
BplI GAGNNNNNCTC 4 cut(s) 542, 574, 641, 673
BpmI CTGGAG 2 cut(s) 186, 1155
BpuMI CCSGG 1 cut(s) 989
BsaAI YACGTR 2 cut(s) 116, 129
BsaHI GRCGYC 1 cut(s) 687
BsaI GGTCTC 3 cut(s) 365, 788, 997
BsaJI CCNNGG 7 cut(s) 11, 631, 732, 846, 934, 996, 1189
BsaWI WCCGGW 1 cut(s) 482
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bse1I ACTGG 5 cut(s) 425, 899, 1055, 1163, 1172
BseAI TCCGGA 1 cut(s) 482
BseBI CCWGG 2 cut(s) 632, 854
BseDI CCNNGG 7 cut(s) 11, 631, 732, 846, 934, 996, 1189
BseGI GGATG 3 cut(s) 497, 796, 1063
BseMII CTCAG 4 cut(s) 156, 239, 245, 358
BseNI ACTGG 5 cut(s) 425, 899, 1055, 1163, 1172
BseSI GKGCMC 4 cut(s) 27, 989, 1028, 1188
BseXI GCAGC 2 cut(s) 19, 1044
BseYI CCCAGC 1 cut(s) 488
BsgI GTGCAG 2 cut(s) 590, 1126
Bsh1236I CGCG 1 cut(s) 1016
BshFI GGCC 5 cut(s) 52, 392, 425, 899, 1001
BsiHKAI GWGCWC 3 cut(s) 239, 1028, 1188
BsiSI CCGG 3 cut(s) 483, 989, 1002
BslFI GGGAC 2 cut(s) 157, 916
BsmAI GTCTC 6 cut(s) 228, 339, 345, 365, 788, 997
BsmFI GGGAC 2 cut(s) 157, 916
BsmI GAATGC 1 cut(s) 889
BsnI GGCC 5 cut(s) 52, 392, 425, 899, 1001
Bso31I GGTCTC 3 cut(s) 365, 788, 997
Bsp1286I GDGCHC 5 cut(s) 27, 239, 989, 1028, 1188
Bsp13I TCCGGA 1 cut(s) 482
Bsp19I CCATGG 1 cut(s) 934
BspACI CCGC 4 cut(s) 120, 909, 913, 1201
BspANI GGCC 5 cut(s) 52, 392, 425, 899, 1001
BspCNI CTCAG 4 cut(s) 155, 238, 244, 357
BspEI TCCGGA 1 cut(s) 482
BspFNI CGCG 1 cut(s) 1016
BspLI GGNNCC 8 cut(s) 172, 173, 651, 764, 932, 940, 1006, 1160
BspQI GCTCTTC 1 cut(s) 301
BspTI CTTAAG 1 cut(s) 289
BspTNI GGTCTC 3 cut(s) 365, 788, 997
BsrI ACTGG 5 cut(s) 425, 899, 1055, 1163, 1172
BssECI CCNNGG 7 cut(s) 11, 631, 732, 846, 934, 996, 1189
BssNAI GTATAC 1 cut(s) 868
BssNI GRCGYC 1 cut(s) 687
BssSI CACGAG 1 cut(s) 218
BssT1I CCWWGG 3 cut(s) 11, 846, 934
Bst1107I GTATAC 1 cut(s) 868
Bst2BI CACGAG 1 cut(s) 218
Bst2UI CCWGG 2 cut(s) 632, 854
Bst4CI ACNGT 4 cut(s) 532, 571, 599, 1193
Bst6I CTCTTC 1 cut(s) 301
BstACI GRCGYC 1 cut(s) 687
BstAFI CTTAAG 1 cut(s) 289
BstBAI YACGTR 2 cut(s) 116, 129
BstC8I GCNNGC 3 cut(s) 54, 452, 911
BstDEI CTNAG 5 cut(s) 142, 225, 231, 282, 344
BstDSI CCRYGG 4 cut(s) 732, 934, 996, 1189
BstEII GGTNACC 1 cut(s) 1193
BstF5I GGATG 3 cut(s) 497, 796, 1063
BstFNI CGCG 1 cut(s) 1016
BstH2I RGCGCY 2 cut(s) 126, 772
BstHHI GCGC 4 cut(s) 41, 125, 771, 1037
BstMAI GTCTC 6 cut(s) 228, 339, 345, 365, 788, 997
BstMWI GCNNNNNNNGC 4 cut(s) 412, 1013, 1032, 1075
BstNI CCWGG 2 cut(s) 632, 854
BstNSI RCATGY 1 cut(s) 517
BstPI GGTNACC 1 cut(s) 1193
BstSCI CCNGG 3 cut(s) 630, 852, 987
BstSLI GKGCMC 4 cut(s) 27, 989, 1028, 1188
BstUI CGCG 1 cut(s) 1016
BstV1I GCAGC 2 cut(s) 19, 1044
BstZ17I GTATAC 1 cut(s) 868
BsuRI GGCC 5 cut(s) 52, 392, 425, 899, 1001
BtgI CCRYGG 4 cut(s) 732, 934, 996, 1189
BtrI CACGTC 1 cut(s) 151
BtsCI GGATG 3 cut(s) 497, 796, 1063
BtsI GCAGTG 2 cut(s) 573, 602
BtsIMutI CAGTG 4 cut(s) 352, 573, 602, 1143
Cac8I GCNNGC 3 cut(s) 54, 452, 911
CfoI GCGC 4 cut(s) 41, 125, 771, 1037
Cfr13I GGNCC 7 cut(s) 171, 423, 599, 850, 897, 930, 938
CseI GACGC 1 cut(s) 676
Csp6I GTAC 4 cut(s) 511, 533, 809, 1048
CviAII CATG 5 cut(s) 331, 514, 820, 935, 1126
CviQI GTAC 4 cut(s) 511, 533, 809, 1048
DdeI CTNAG 5 cut(s) 142, 225, 231, 282, 344
DraI TTTAAA 1 cut(s) 90
DrdI GACNNNNNNGTC 1 cut(s) 928
DseDI GACNNNNNNGTC 1 cut(s) 928
EaeI YGGCCR 3 cut(s) 50, 390, 999
Eam1104I CTCTTC 1 cut(s) 301
EarI CTCTTC 1 cut(s) 301
Ecl136II GAGCTC 1 cut(s) 237
Eco130I CCWWGG 3 cut(s) 11, 846, 934
Eco24I GRGCYC 1 cut(s) 239
Eco31I GGTCTC 3 cut(s) 365, 788, 997
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 5 cut(s) 171, 599, 850, 930, 938
Eco53kI GAGCTC 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 300
Eco91I GGTNACC 1 cut(s) 1193
EcoICRI GAGCTC 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 171
EcoO65I GGTNACC 1 cut(s) 1193
EcoRII CCWGG 2 cut(s) 630, 852
EcoRV GATATC 1 cut(s) 109
EcoT14I CCWWGG 3 cut(s) 11, 846, 934
EcoT38I GRGCYC 1 cut(s) 239
ErhI CCWWGG 3 cut(s) 11, 846, 934
FaeI CATG 5 cut(s) 334, 517, 823, 938, 1129
FalI AAGNNNNNCTT 4 cut(s) 283, 315, 438, 470
FaqI GGGAC 2 cut(s) 157, 916
FatI CATG 5 cut(s) 330, 513, 819, 934, 1125
FauI CCCGC 2 cut(s) 902, 906
FauNDI CATATG 1 cut(s) 462
FblI GTMKAC 1 cut(s) 867
Fnu4HI GCNGC 3 cut(s) 8, 121, 1033
FokI GGATG 3 cut(s) 484, 803, 1070
FriOI GRGCYC 1 cut(s) 239
Fsp4HI GCNGC 3 cut(s) 8, 121, 1033
FspBI CTAG 4 cut(s) 12, 785, 1155, 1207
GlaI GCGC 4 cut(s) 40, 124, 770, 1036
GluI GCNGC 3 cut(s) 8, 121, 1033
GsaI CCCAGC 1 cut(s) 492
GsuI CTGGAG 2 cut(s) 186, 1155
HaeII RGCGCY 2 cut(s) 126, 772
HaeIII GGCC 5 cut(s) 52, 392, 425, 899, 1001
HapII CCGG 3 cut(s) 483, 989, 1002
HgaI GACGC 1 cut(s) 676
HhaI GCGC 4 cut(s) 41, 125, 771, 1037
Hin1I GRCGYC 1 cut(s) 687
Hin1II CATG 5 cut(s) 334, 517, 823, 938, 1129
Hin6I GCGC 4 cut(s) 39, 123, 769, 1035
HinP1I GCGC 4 cut(s) 39, 123, 769, 1035
HinfI GANTC 5 cut(s) 140, 155, 177, 229, 557
HpaII CCGG 3 cut(s) 483, 989, 1002
HphI GGTGA 2 cut(s) 566, 1187
Hpy166II GTNNAC 7 cut(s) 154, 602, 811, 868, 1026, 1087, 1186
Hpy188I TCNGA 7 cut(s) 145, 228, 234, 430, 595, 666, 1075
Hpy188III TCNNGA 7 cut(s) 165, 174, 181, 483, 760, 880, 919
Hpy8I GTNNAC 7 cut(s) 154, 602, 811, 868, 1026, 1087, 1186
Hpy99I CGWCG 1 cut(s) 692
HpyAV CCTTC 3 cut(s) 282, 440, 850
HpyCH4III ACNGT 4 cut(s) 532, 571, 599, 1193
HpyCH4IV ACGT 4 cut(s) 115, 128, 150, 1083
HpyCH4V TGCA 5 cut(s) 607, 950, 1026, 1143, 1186
HpyF10VI GCNNNNNNNGC 4 cut(s) 412, 1013, 1032, 1075
HpyF3I CTNAG 5 cut(s) 142, 225, 231, 282, 344
HpySE526I ACGT 4 cut(s) 115, 128, 150, 1083
Hsp92I GRCGYC 1 cut(s) 687
Hsp92II CATG 5 cut(s) 334, 517, 823, 938, 1129
HspAI GCGC 4 cut(s) 39, 123, 769, 1035
KflI GGGWCCC 1 cut(s) 171
Kpn2I TCCGGA 1 cut(s) 482
LguI GCTCTTC 1 cut(s) 301
LmnI GCTCC 4 cut(s) 67, 485, 649, 1004
Lsp1109I GCAGC 2 cut(s) 19, 1044
LweI GCATC 1 cut(s) 959
MaeI CTAG 4 cut(s) 12, 785, 1155, 1207
MaeII ACGT 4 cut(s) 115, 128, 150, 1083
MaeIII GTNAC 6 cut(s) 74, 526, 554, 571, 1165, 1193
MboII GAAGA 6 cut(s) 244, 267, 288, 291, 449, 1030
MhlI GDGCHC 5 cut(s) 27, 239, 989, 1028, 1188
MlsI TGGCCA 1 cut(s) 52
MluCI AATT 5 cut(s) 101, 190, 395, 475, 801
MluNI TGGCCA 1 cut(s) 52
MlyI GAGTC 5 cut(s) 134, 149, 171, 223, 551
MmeI TCCRAC 1 cut(s) 453
MnlI CCTC 8 cut(s) 40, 126, 161, 289, 331, 436, 544, 683
Mox20I TGGCCA 1 cut(s) 52
MroI TCCGGA 1 cut(s) 482
MscI TGGCCA 1 cut(s) 52
MseI TTAA 4 cut(s) 89, 290, 540, 1113
MslI CAYNNNNRTG 1 cut(s) 818
Msp20I TGGCCA 1 cut(s) 52
MspA1I CMGCKG 1 cut(s) 1102
MspCI CTTAAG 1 cut(s) 289
MspI CCGG 3 cut(s) 483, 989, 1002
MspR9I CCNGG 3 cut(s) 632, 854, 989
Mva1269I GAATGC 1 cut(s) 889
MvaI CCWGG 2 cut(s) 632, 854
MvnI CGCG 1 cut(s) 1016
MwoI GCNNNNNNNGC 4 cut(s) 412, 1013, 1032, 1075
NciI CCSGG 1 cut(s) 989
NcoI CCATGG 1 cut(s) 934
NdeI CATATG 1 cut(s) 462
NlaIII CATG 5 cut(s) 334, 517, 823, 938, 1129
NlaIV GGNNCC 8 cut(s) 172, 173, 651, 764, 932, 940, 1006, 1160
NmeAIII GCCGAG 1 cut(s) 368
NmuCI GTSAC 5 cut(s) 526, 554, 571, 1165, 1193
NspI RCATGY 1 cut(s) 517
PciI ACATGT 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 301
PctI GAATGC 1 cut(s) 889
PflMI CCANNNNNTGG 4 cut(s) 759, 859, 1102, 1126
PkrI GCNGC 3 cut(s) 9, 122, 1034
PleI GAGTC 5 cut(s) 134, 149, 171, 223, 551
PpsI GAGTC 5 cut(s) 134, 149, 171, 223, 551
Ppu21I YACGTR 2 cut(s) 116, 129
PpuMI RGGWCCY 1 cut(s) 171
PscI ACATGT 1 cut(s) 513
PsiI TTATAA 1 cut(s) 957
Psp124BI GAGCTC 1 cut(s) 239
Psp5II RGGWCCY 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 630, 852
PspEI GGTNACC 1 cut(s) 1193
PspFI CCCAGC 1 cut(s) 488
PspGI CCWGG 2 cut(s) 630, 852
PspN4I GGNNCC 8 cut(s) 172, 173, 651, 764, 932, 940, 1006, 1160
PspPI GGNCC 7 cut(s) 171, 423, 599, 850, 897, 930, 938
PspPPI RGGWCCY 1 cut(s) 171
PvuII CAGCTG 1 cut(s) 1102
RsaI GTAC 4 cut(s) 512, 534, 810, 1049
RsaNI GTAC 4 cut(s) 511, 533, 809, 1048
RseI CAYNNNNRTG 1 cut(s) 818
SacI GAGCTC 1 cut(s) 239
SapI GCTCTTC 1 cut(s) 301
SaqAI TTAA 4 cut(s) 89, 290, 540, 1113
SatI GCNGC 3 cut(s) 8, 121, 1033
Sau96I GGNCC 7 cut(s) 171, 423, 599, 850, 897, 930, 938
ScaI AGTACT 1 cut(s) 1049
SchI GAGTC 5 cut(s) 134, 149, 171, 223, 551
ScrFI CCNGG 3 cut(s) 632, 854, 989
SduI GDGCHC 5 cut(s) 27, 239, 989, 1028, 1188
SfaNI GCATC 1 cut(s) 959
SinI GGWCC 5 cut(s) 171, 599, 850, 930, 938
SmiMI CAYNNNNRTG 1 cut(s) 818
SmlI CTYRAG 1 cut(s) 289
SmoI CTYRAG 1 cut(s) 289
Sse9I AATT 5 cut(s) 101, 190, 395, 475, 801
SsiI CCGC 4 cut(s) 120, 909, 913, 1201
SspMI CTAG 4 cut(s) 12, 785, 1155, 1207
SstI GAGCTC 1 cut(s) 239
StyD4I CCNGG 3 cut(s) 630, 852, 987
StyI CCWWGG 3 cut(s) 11, 846, 934
TaaI ACNGT 4 cut(s) 532, 571, 599, 1193
TaiI ACGT 4 cut(s) 118, 131, 153, 1086
TaqI TCGA 5 cut(s) 98, 323, 399, 471, 624
TaqII GACCGA 1 cut(s) 938
TasI AATT 5 cut(s) 101, 190, 395, 475, 801
TatI WGTACW 2 cut(s) 510, 1047
TauI GCSGC 1 cut(s) 123
Tru1I TTAA 4 cut(s) 89, 290, 540, 1113
Tru9I TTAA 4 cut(s) 89, 290, 540, 1113
TscAI CASTG 4 cut(s) 352, 580, 609, 1150
TseFI GTSAC 5 cut(s) 526, 554, 571, 1165, 1193
TseI GCWGC 2 cut(s) 7, 1032
Tsp45I GTSAC 5 cut(s) 526, 554, 571, 1165, 1193
TspDTI ATGAA 3 cut(s) 449, 479, 486
TspGWI ACGGA 2 cut(s) 749, 985
TspRI CASTG 4 cut(s) 352, 580, 609, 1150
Van91I CCANNNNNTGG 4 cut(s) 759, 859, 1102, 1126
Vha464I CTTAAG 1 cut(s) 289
VneI GTGCAC 2 cut(s) 1024, 1184
VpaK11BI GGWCC 5 cut(s) 171, 599, 850, 930, 938
XapI RAATTY 2 cut(s) 101, 395
XceI RCATGY 1 cut(s) 517
XcmI CCANNNNNNNNNTGG 1 cut(s) 60
XmaJI CCTAGG 1 cut(s) 11
XmiI GTMKAC 1 cut(s) 867
XspI CTAG 4 cut(s) 12, 785, 1155, 1207
ZrmI AGTACT 1 cut(s) 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.