Rmu_sc0000690.1_g000047

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000690.1
N/A
Physical Location & Seq
Forward (+)
144623 .. 147119
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 588 bp
atgcatcttcaatttatttccttgcagggccttgttgtgccagttatccgcaatgctgataagatgaattgtggtgagatagagaaggagatcaattcgcttgcaaagaaggcaaatgaggaacgtctttcaattgatgatatggctggaggttcatttacaatatccaatggtggattttatggaagccttataagtaccctcatcatcaaccctcaggtactattggctaacgaaccacgatgcctacaggaggtacctgaggcagaatacttcaacatatatgatgctctcagattaacgagaaagcatccagacaaggtgaaacggatcgatcagggatccgaactggagagagatccaaatgacggattcgaatttgaagctacaggaatcggggtattggattggtgggttgtgatgatcgaagaactccccgaaattggacttgaatatcttaaccccgctgacataatcgaagaaaaatcggcggggatggtggtgactgcggtggtggcgccgttgggaacgaatgcttctcatggtggccggccggtgttcacggtggctgggaatatgtgtgaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

195

Amino Acids

21.45

Weight (kDa)

4.6

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 9 - 73 8.5e-20 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 194
Acc65I GGTACC 1 cut(s) 256
AccB1I GGYRCC 2 cut(s) 256, 517
AciI CCGC 4 cut(s) 49, 465, 491, 509
AclWI GGATC 4 cut(s) 336, 338, 349, 353
AcoI YGGCCR 2 cut(s) 547, 551
AcsI RAATTY 1 cut(s) 377
AcyI GRCGYC 1 cut(s) 518
AfaI GTAC 3 cut(s) 199, 222, 258
AfiI CCNNNNNNNGG 3 cut(s) 253, 368, 443
AgsI TTSAA 5 cut(s) 11, 132, 277, 383, 452
AluBI AGCT 1 cut(s) 386
AluI AGCT 1 cut(s) 386
AlwI GGATC 4 cut(s) 336, 338, 349, 353
AoxI GGCC 3 cut(s) 28, 547, 551
ApoI RAATTY 1 cut(s) 377
Asp718I GGTACC 1 cut(s) 256
AspLEI GCGC 1 cut(s) 520
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 3 cut(s) 86, 334, 514
AsuII TTCGAA 1 cut(s) 375
AxyI CCTNAGG 2 cut(s) 216, 261
BamHI GGATCC 1 cut(s) 341
BanI GGYRCC 2 cut(s) 256, 517
BccI CCATC 1 cut(s) 490
BceAI ACGGC 1 cut(s) 505
BfmI CTRYAG 2 cut(s) 248, 387
BfoI RGCGCY 1 cut(s) 521
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 3 cut(s) 258, 343, 519
BmsI GCATC 4 cut(s) 13, 233, 277, 319
BpmI CTGGAG 2 cut(s) 168, 371
Bpu14I TTCGAA 1 cut(s) 375
Bsa29I ATCGAT 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 518
Bsc4I CCNNNNNNNGG 3 cut(s) 253, 368, 443
Bse118I RCCGGY 2 cut(s) 549, 553
Bse1I ACTGG 2 cut(s) 41, 354
Bse21I CCTNAGG 2 cut(s) 216, 261
Bse3DI GCAATG 1 cut(s) 58
BseCI ATCGAT 1 cut(s) 333
BseGI GGATG 2 cut(s) 310, 501
BseLI CCNNNNNNNGG 3 cut(s) 253, 368, 443
BseMI GCAATG 1 cut(s) 58
BseMII CTCAG 3 cut(s) 230, 252, 307
BseNI ACTGG 2 cut(s) 41, 354
BseX3I CGGCCG 1 cut(s) 551
BseYI CCCAGC 1 cut(s) 569
Bsh1285I CGRYCG 1 cut(s) 554
BshFI GGCC 3 cut(s) 30, 549, 553
BshNI GGYRCC 2 cut(s) 256, 517
BshVI ATCGAT 1 cut(s) 333
BsiEI CGRYCG 1 cut(s) 554
BsiSI CCGG 2 cut(s) 550, 554
BslI CCNNNNNNNGG 3 cut(s) 253, 368, 443
BsmI GAATGC 1 cut(s) 538
BsnI GGCC 3 cut(s) 30, 549, 553
Bsp119I TTCGAA 1 cut(s) 375
Bsp143I GATC 6 cut(s) 90, 330, 334, 341, 358, 423
BspACI CCGC 4 cut(s) 49, 465, 491, 509
BspANI GGCC 3 cut(s) 30, 549, 553
BspCNI CTCAG 3 cut(s) 229, 253, 306
BspDI ATCGAT 1 cut(s) 333
BspLI GGNNCC 3 cut(s) 258, 343, 519
BspPI GGATC 4 cut(s) 336, 338, 349, 353
BspT104I TTCGAA 1 cut(s) 375
BspT107I GGYRCC 2 cut(s) 256, 517
BsrDI GCAATG 1 cut(s) 58
BsrFI RCCGGY 2 cut(s) 549, 553
BsrI ACTGG 2 cut(s) 41, 354
BssAI RCCGGY 2 cut(s) 549, 553
BssMI GATC 6 cut(s) 90, 330, 334, 341, 358, 423
BssNI GRCGYC 1 cut(s) 518
Bst4CI ACNGT 1 cut(s) 565
BstACI GRCGYC 1 cut(s) 518
BstBI TTCGAA 1 cut(s) 375
BstC8I GCNNGC 2 cut(s) 102, 551
BstDEI CTNAG 3 cut(s) 216, 261, 293
BstENI CCTNNNNNAGG 1 cut(s) 251
BstF5I GGATG 2 cut(s) 310, 501
BstH2I RGCGCY 1 cut(s) 521
BstHHI GCGC 1 cut(s) 520
BstKTI GATC 6 cut(s) 93, 333, 337, 344, 361, 426
BstMBI GATC 6 cut(s) 90, 330, 334, 341, 358, 423
BstMCI CGRYCG 1 cut(s) 554
BstMWI GCNNNNNNNGC 2 cut(s) 110, 515
BstSFI CTRYAG 2 cut(s) 248, 387
BstX2I RGATCY 2 cut(s) 341, 358
BstYI RGATCY 2 cut(s) 341, 358
BstZI CGGCCG 1 cut(s) 551
Bsu15I ATCGAT 1 cut(s) 333
Bsu36I CCTNAGG 2 cut(s) 216, 261
BsuRI GGCC 3 cut(s) 30, 549, 553
BsuTUI ATCGAT 1 cut(s) 333
BtsCI GGATG 2 cut(s) 310, 501
Cac8I GCNNGC 2 cut(s) 102, 551
CfoI GCGC 1 cut(s) 520
Cfr10I RCCGGY 2 cut(s) 549, 553
Cfr13I GGNCC 1 cut(s) 28
ClaI ATCGAT 1 cut(s) 333
Csp6I GTAC 3 cut(s) 198, 221, 257
CviAII CATG 1 cut(s) 542
CviJI RGCY 8 cut(s) 30, 146, 189, 230, 386, 549, 553, 569
CviKI_1 RGCY 8 cut(s) 30, 146, 189, 230, 386, 549, 553, 569
CviQI GTAC 3 cut(s) 198, 221, 257
DdeI CTNAG 3 cut(s) 216, 261, 293
DinI GGCGCC 1 cut(s) 519
DpnI GATC 6 cut(s) 92, 332, 336, 343, 360, 425
DpnII GATC 6 cut(s) 90, 330, 334, 341, 358, 423
EaeI YGGCCR 2 cut(s) 547, 551
EagI CGGCCG 1 cut(s) 551
EclXI CGGCCG 1 cut(s) 551
Eco52I CGGCCG 1 cut(s) 551
Eco81I CCTNAGG 2 cut(s) 216, 261
EcoNI CCTNNNNNAGG 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 28
EcoT22I ATGCAT 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 519
EheI GGCGCC 1 cut(s) 519
FaeI CATG 1 cut(s) 545
FaiI YATR 9 cut(s) 143, 183, 194, 281, 283, 285, 473, 543, 578
FatI CATG 1 cut(s) 541
FauI CCCGC 2 cut(s) 472, 484
FokI GGATG 2 cut(s) 297, 508
FseI GGCCGGCC 1 cut(s) 553
GlaI GCGC 1 cut(s) 519
GsaI CCCAGC 1 cut(s) 573
GsuI CTGGAG 2 cut(s) 168, 371
HaeII RGCGCY 1 cut(s) 521
HaeIII GGCC 3 cut(s) 30, 549, 553
HapII CCGG 2 cut(s) 550, 554
HhaI GCGC 1 cut(s) 520
Hin1I GRCGYC 1 cut(s) 518
Hin1II CATG 1 cut(s) 545
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HinfI GANTC 2 cut(s) 372, 393
HpaII CCGG 2 cut(s) 550, 554
HphI GGTGA 3 cut(s) 86, 334, 514
Hpy166II GTNNAC 1 cut(s) 561
Hpy188I TCNGA 2 cut(s) 296, 346
Hpy188III TCNNGA 1 cut(s) 314
Hpy8I GTNNAC 1 cut(s) 561
HpyAV CCTTC 2 cut(s) 79, 103
HpyCH4III ACNGT 1 cut(s) 565
HpyCH4IV ACGT 1 cut(s) 124
HpyCH4V TGCA 3 cut(s) 4, 25, 104
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 515
HpyF3I CTNAG 3 cut(s) 216, 261, 293
HpySE526I ACGT 1 cut(s) 124
Hsp92I GRCGYC 1 cut(s) 518
Hsp92II CATG 1 cut(s) 545
HspAI GCGC 1 cut(s) 518
KasI GGCGCC 1 cut(s) 517
KpnI GGTACC 1 cut(s) 260
KroI GCCGGC 1 cut(s) 549
KroNI GCCGGC 1 cut(s) 551
Kzo9I GATC 6 cut(s) 90, 330, 334, 341, 358, 423
LweI GCATC 4 cut(s) 13, 233, 277, 319
MaeII ACGT 1 cut(s) 124
MaeIII GTNAC 1 cut(s) 502
MalI GATC 6 cut(s) 92, 332, 336, 343, 360, 425
MboI GATC 6 cut(s) 90, 330, 334, 341, 358, 423
MboII GAAGA 2 cut(s) 440, 491
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 2 cut(s) 341, 358
MluCI AATT 6 cut(s) 11, 67, 94, 132, 377, 441
Mly113I GGCGCC 1 cut(s) 518
MnlI CCTC 6 cut(s) 112, 143, 212, 225, 247, 256
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 549
MseI TTAA 2 cut(s) 299, 459
MspA1I CMGCKG 1 cut(s) 467
MspI CCGG 2 cut(s) 550, 554
MunI CAATTG 1 cut(s) 132
Mva1269I GAATGC 1 cut(s) 538
MwoI GCNNNNNNNGC 2 cut(s) 110, 515
NaeI GCCGGC 1 cut(s) 551
NarI GGCGCC 1 cut(s) 518
NdeII GATC 6 cut(s) 90, 330, 334, 341, 358, 423
NgoMIV GCCGGC 1 cut(s) 549
NlaIII CATG 1 cut(s) 545
NlaIV GGNNCC 3 cut(s) 258, 343, 519
NmuCI GTSAC 1 cut(s) 502
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 375
PctI GAATGC 1 cut(s) 538
PdiI GCCGGC 1 cut(s) 551
PfeI GAWTC 2 cut(s) 372, 393
PluTI GGCGCC 1 cut(s) 521
PsiI TTATAA 1 cut(s) 194
PspFI CCCAGC 1 cut(s) 569
PspN4I GGNNCC 3 cut(s) 258, 343, 519
PspPI GGNCC 1 cut(s) 28
PsuI RGATCY 2 cut(s) 341, 358
RigI GGCCGGCC 1 cut(s) 553
RsaI GTAC 3 cut(s) 199, 222, 258
RsaNI GTAC 3 cut(s) 198, 221, 257
SaqAI TTAA 2 cut(s) 299, 459
Sau3AI GATC 6 cut(s) 90, 330, 334, 341, 358, 423
Sau96I GGNCC 1 cut(s) 28
SetI ASST 7 cut(s) 127, 154, 222, 258, 262, 324, 388
SfaNI GCATC 4 cut(s) 13, 233, 277, 319
SfcI CTRYAG 2 cut(s) 248, 387
SfoI GGCGCC 1 cut(s) 519
SfuI TTCGAA 1 cut(s) 375
Sse9I AATT 6 cut(s) 11, 67, 94, 132, 377, 441
SsiI CCGC 4 cut(s) 49, 465, 491, 509
SspDI GGCGCC 1 cut(s) 517
TaaI ACNGT 1 cut(s) 565
TaiI ACGT 1 cut(s) 127
TaqI TCGA 4 cut(s) 333, 375, 426, 477
TasI AATT 6 cut(s) 11, 67, 94, 132, 377, 441
TfiI GAWTC 2 cut(s) 372, 393
Tru1I TTAA 2 cut(s) 299, 459
Tru9I TTAA 2 cut(s) 299, 459
TseFI GTSAC 1 cut(s) 502
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 2 cut(s) 80, 144
TspGWI ACGGA 2 cut(s) 343, 384
XagI CCTNNNNNAGG 1 cut(s) 251
XapI RAATTY 1 cut(s) 377
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.