Rmu_sc0000706.1_g000009

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000706.1
N/A
Physical Location & Seq
Reverse (-)
36432 .. 39429
2998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000706.1_g000009.1.cds

Sequence Viewer

Length: 732 bp
atggagtgtagaaacacactggggcaaatagtcttcgccgtgttctttgatgttgatccttcagatgtcaggagacagagtggtacttttgcacaggcactcgatcggaaacataaagatatagatgaagatgtaaggtgcaaatttggagagcagctcgtacagaagctgcagatttgtctggctgggatcttcaaaacacttccgatggacttgcatgtagctgaccaccaagttggagtagactctcgcgtggaagatattagttcttatttaggtgttggaatattgcatgatgttcgcatggttggaatttggggtatgggtgaaattgggaaaactacaattgccaaggccctttacaacaaacttcgccataactttgaagcttcaagtttcctcgccaacattagggaaactgccaaccaacacaatggactttggtggcgcgatttgggtcgtaagaggtgcagcaaggttgtgaacgaggtggccatttcgagtctctggcgatttcttatttttcggccacttccagccgcaccaccacccatttcttggagttctcttcatcttgggacgagccatggcagtgggcaatggcaattttgccagattacgccaaatatggtggttgaccggcggagcgtggggcccaagcgtagccgcctgtggtggtgcatgaaggcgcgtccggttgttcctggtggctggtgtgggtctgttgggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

243

Amino Acids

27.38

Weight (kDa)

9.42

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 558
AccI GTMKAC 1 cut(s) 243
AccII CGCG 3 cut(s) 252, 450, 691
AciI CCGC 3 cut(s) 540, 643, 667
AclWI GGATC 2 cut(s) 50, 197
AcoI YGGCCR 2 cut(s) 492, 527
AcsI RAATTY 2 cut(s) 143, 312
AcuI CTGAAG 1 cut(s) 45
AfaI GTAC 2 cut(s) 85, 162
AfiI CCNNNNNNNGG 2 cut(s) 411, 558
AgsI TTSAA 3 cut(s) 196, 386, 393
AjnI CCWGG 1 cut(s) 703
AluBI AGCT 4 cut(s) 157, 169, 224, 389
AluI AGCT 4 cut(s) 157, 169, 224, 389
Alw26I GTCTC 2 cut(s) 67, 509
AlwI GGATC 2 cut(s) 50, 197
AlwNI CAGNNNCTG 1 cut(s) 169
AoxI GGCC 4 cut(s) 354, 492, 527, 653
ApaI GGGCCC 1 cut(s) 657
ApeKI GCWGC 3 cut(s) 154, 169, 471
ApoI RAATTY 2 cut(s) 143, 312
AspLEI GCGC 2 cut(s) 450, 691
AspS9I GGNCC 3 cut(s) 355, 653, 654
AsuHPI GGTGA 1 cut(s) 338
BaeGI GKGCMC 1 cut(s) 657
BalI TGGCCA 1 cut(s) 494
BanII GRGCYC 1 cut(s) 657
BbsI GAAGAC 1 cut(s) 25
BbvI GCAGC 3 cut(s) 156, 166, 483
BccI CCATC 1 cut(s) 202
BceAI ACGGC 1 cut(s) 23
BcgI CGANNNNNNTGC 4 cut(s) 196, 230, 281, 315
BciT130I CCWGG 1 cut(s) 705
BcoDI GTCTC 2 cut(s) 67, 509
BfmI CTRYAG 1 cut(s) 170
BisI GCNGC 5 cut(s) 155, 170, 472, 540, 667
BlsI GCNGC 5 cut(s) 156, 171, 473, 541, 668
Bme1390I CCNGG 1 cut(s) 705
BmgT120I GGNCC 3 cut(s) 355, 653, 654
BmiI GGNNCC 2 cut(s) 654, 655
BmrFI CCNGG 1 cut(s) 705
BmrI ACTGGG 1 cut(s) 29
BmuI ACTGGG 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 25
BplI GAGNNNNNCTC 2 cut(s) 141, 173
BsaBI GATNNNNATC 1 cut(s) 54
BsaJI CCNNGG 2 cut(s) 351, 586
BsaWI WCCGGW 1 cut(s) 694
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 2 cut(s) 411, 558
Bse118I RCCGGY 1 cut(s) 639
Bse1I ACTGG 1 cut(s) 24
Bse3DI GCAATG 1 cut(s) 605
Bse8I GATNNNNATC 1 cut(s) 54
BseBI CCWGG 1 cut(s) 705
BseDI CCNNGG 2 cut(s) 351, 586
BseJI GATNNNNATC 1 cut(s) 54
BseLI CCNNNNNNNGG 2 cut(s) 411, 558
BseMI GCAATG 1 cut(s) 605
BseNI ACTGG 1 cut(s) 24
BseSI GKGCMC 1 cut(s) 657
BseXI GCAGC 3 cut(s) 156, 166, 483
BseYI CCCAGC 1 cut(s) 185
BsgI GTGCAG 1 cut(s) 490
Bsh1236I CGCG 3 cut(s) 252, 450, 691
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 4 cut(s) 356, 494, 529, 655
BsiEI CGRYCG 1 cut(s) 106
BsiSI CCGG 2 cut(s) 640, 695
BslFI GGGAC 1 cut(s) 592
BslI CCNNNNNNNGG 2 cut(s) 411, 558
BsmAI GTCTC 2 cut(s) 67, 509
BsmFI GGGAC 1 cut(s) 592
BsnI GGCC 4 cut(s) 356, 494, 529, 655
Bsp120I GGGCCC 1 cut(s) 653
Bsp1286I GDGCHC 1 cut(s) 657
Bsp143I GATC 3 cut(s) 55, 103, 189
Bsp19I CCATGG 1 cut(s) 586
BspACI CCGC 3 cut(s) 540, 643, 667
BspANI GGCC 4 cut(s) 356, 494, 529, 655
BspFNI CGCG 3 cut(s) 252, 450, 691
BspLI GGNNCC 2 cut(s) 654, 655
BspMAI CTGCAG 1 cut(s) 174
BspPI GGATC 2 cut(s) 50, 197
BsrDI GCAATG 1 cut(s) 605
BsrFI RCCGGY 1 cut(s) 639
BsrI ACTGG 1 cut(s) 24
BssAI RCCGGY 1 cut(s) 639
BssECI CCNNGG 2 cut(s) 351, 586
BssMI GATC 3 cut(s) 55, 103, 189
BssT1I CCWWGG 2 cut(s) 351, 586
Bst2UI CCWGG 1 cut(s) 705
Bst6I CTCTTC 1 cut(s) 573
BstDSI CCRYGG 1 cut(s) 586
BstFNI CGCG 3 cut(s) 252, 450, 691
BstHHI GCGC 2 cut(s) 450, 691
BstKTI GATC 3 cut(s) 58, 106, 192
BstMAI GTCTC 2 cut(s) 67, 509
BstMBI GATC 3 cut(s) 55, 103, 189
BstMCI CGRYCG 1 cut(s) 106
BstNI CCWGG 1 cut(s) 705
BstNSI RCATGY 1 cut(s) 221
BstSCI CCNGG 1 cut(s) 703
BstSFI CTRYAG 1 cut(s) 170
BstSLI GKGCMC 1 cut(s) 657
BstUI CGCG 3 cut(s) 252, 450, 691
BstV1I GCAGC 3 cut(s) 156, 166, 483
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 189
BstXI CCANNNNNNTGG 3 cut(s) 236, 434, 593
BstYI RGATCY 1 cut(s) 189
BsuRI GGCC 4 cut(s) 356, 494, 529, 655
BtgI CCRYGG 1 cut(s) 586
BtsI GCAGTG 1 cut(s) 598
BtsIMutI CAGTG 2 cut(s) 17, 598
CaiI CAGNNNCTG 1 cut(s) 169
CfoI GCGC 2 cut(s) 450, 691
Cfr10I RCCGGY 1 cut(s) 639
Cfr13I GGNCC 3 cut(s) 355, 653, 654
CseI GACGC 1 cut(s) 680
Csp6I GTAC 2 cut(s) 84, 161
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
CviAII CATG 5 cut(s) 218, 293, 304, 587, 682
CviQI GTAC 2 cut(s) 84, 161
DpnI GATC 3 cut(s) 57, 105, 191
DpnII GATC 3 cut(s) 55, 103, 189
EaeI YGGCCR 2 cut(s) 492, 527
Eam1104I CTCTTC 1 cut(s) 573
EarI CTCTTC 1 cut(s) 573
EciI GGCGGA 1 cut(s) 658
Eco130I CCWWGG 2 cut(s) 351, 586
Eco24I GRGCYC 1 cut(s) 657
Eco57I CTGAAG 1 cut(s) 45
EcoO109I RGGNCCY 2 cut(s) 355, 653
EcoRII CCWGG 1 cut(s) 703
EcoT14I CCWWGG 2 cut(s) 351, 586
EcoT38I GRGCYC 1 cut(s) 657
ErhI CCWWGG 2 cut(s) 351, 586
FaeI CATG 5 cut(s) 221, 296, 307, 590, 685
FaqI GGGAC 1 cut(s) 592
FatI CATG 5 cut(s) 217, 292, 303, 586, 681
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 5 cut(s) 155, 170, 472, 540, 667
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 5 cut(s) 155, 170, 472, 540, 667
GlaI GCGC 2 cut(s) 449, 690
GluI GCNGC 5 cut(s) 155, 170, 472, 540, 667
GsaI CCCAGC 1 cut(s) 189
HaeIII GGCC 4 cut(s) 356, 494, 529, 655
HapII CCGG 2 cut(s) 640, 695
HgaI GACGC 1 cut(s) 680
HhaI GCGC 2 cut(s) 450, 691
Hin1II CATG 5 cut(s) 221, 296, 307, 590, 685
Hin6I GCGC 2 cut(s) 448, 689
HinP1I GCGC 2 cut(s) 448, 689
HincII GTYRAC 1 cut(s) 637
HindII GTYRAC 1 cut(s) 637
HindIII AAGCTT 1 cut(s) 387
HinfI GANTC 2 cut(s) 245, 502
HpaII CCGG 2 cut(s) 640, 695
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 3 cut(s) 244, 484, 637
Hpy188I TCNGA 3 cut(s) 64, 108, 207
Hpy188III TCNNGA 1 cut(s) 70
Hpy8I GTNNAC 3 cut(s) 244, 484, 637
HpyAV CCTTC 2 cut(s) 69, 679
HpyCH4V TGCA 7 cut(s) 92, 141, 172, 217, 292, 471, 681
Hsp92II CATG 5 cut(s) 221, 296, 307, 590, 685
HspAI GCGC 2 cut(s) 448, 689
Kzo9I GATC 3 cut(s) 55, 103, 189
LmnI GCTCC 1 cut(s) 645
Lsp1109I GCAGC 3 cut(s) 156, 166, 483
MalI GATC 3 cut(s) 57, 105, 191
MboI GATC 3 cut(s) 55, 103, 189
MboII GAAGA 5 cut(s) 25, 140, 184, 269, 560
MfeI CAATTG 1 cut(s) 345
MflI RGATCY 1 cut(s) 189
MhlI GDGCHC 1 cut(s) 657
MlsI TGGCCA 1 cut(s) 494
MluCI AATT 5 cut(s) 143, 312, 330, 345, 605
MluNI TGGCCA 1 cut(s) 494
MlyI GAGTC 2 cut(s) 239, 511
MmeI TCCRAC 3 cut(s) 217, 262, 289
MnlI CCTC 3 cut(s) 410, 459, 481
Mox20I TGGCCA 1 cut(s) 494
MscI TGGCCA 1 cut(s) 494
MslI CAYNNNNRTG 1 cut(s) 591
Msp20I TGGCCA 1 cut(s) 494
MspI CCGG 2 cut(s) 640, 695
MspR9I CCNGG 1 cut(s) 705
MunI CAATTG 1 cut(s) 345
MvaI CCWGG 1 cut(s) 705
MvnI CGCG 3 cut(s) 252, 450, 691
NcoI CCATGG 1 cut(s) 586
NdeII GATC 3 cut(s) 55, 103, 189
NlaIII CATG 5 cut(s) 221, 296, 307, 590, 685
NlaIV GGNNCC 2 cut(s) 654, 655
NspI RCATGY 1 cut(s) 221
PflMI CCANNNNNTGG 1 cut(s) 558
PkrI GCNGC 5 cut(s) 156, 171, 473, 541, 668
Ple19I CGATCG 1 cut(s) 106
PleI GAGTC 2 cut(s) 239, 510
PpsI GAGTC 2 cut(s) 239, 510
Psp6I CCWGG 1 cut(s) 703
PspFI CCCAGC 1 cut(s) 185
PspGI CCWGG 1 cut(s) 703
PspN4I GGNNCC 2 cut(s) 654, 655
PspOMI GGGCCC 1 cut(s) 653
PspPI GGNCC 3 cut(s) 355, 653, 654
PstI CTGCAG 1 cut(s) 174
PstNI CAGNNNCTG 1 cut(s) 169
PsuI RGATCY 1 cut(s) 189
PvuI CGATCG 1 cut(s) 106
RsaI GTAC 2 cut(s) 85, 162
RsaNI GTAC 2 cut(s) 84, 161
RseI CAYNNNNRTG 1 cut(s) 591
SatI GCNGC 5 cut(s) 155, 170, 472, 540, 667
Sau3AI GATC 3 cut(s) 55, 103, 189
Sau96I GGNCC 3 cut(s) 355, 653, 654
SchI GAGTC 2 cut(s) 239, 511
ScrFI CCNGG 1 cut(s) 705
SduI GDGCHC 1 cut(s) 657
SetI ASST 9 cut(s) 140, 159, 171, 226, 280, 391, 470, 480, 492
SfcI CTRYAG 1 cut(s) 170
SmiMI CAYNNNNRTG 1 cut(s) 591
Sse9I AATT 5 cut(s) 143, 312, 330, 345, 605
SsiI CCGC 3 cut(s) 540, 643, 667
SspI AATATT 1 cut(s) 288
StyD4I CCNGG 1 cut(s) 703
StyI CCWWGG 2 cut(s) 351, 586
TaqI TCGA 2 cut(s) 102, 500
TasI AATT 5 cut(s) 143, 312, 330, 345, 605
TauI GCSGC 2 cut(s) 542, 669
TscAI CASTG 2 cut(s) 24, 598
TseI GCWGC 3 cut(s) 154, 169, 471
TspDTI ATGAA 3 cut(s) 141, 560, 698
TspRI CASTG 2 cut(s) 24, 598
Van91I CCANNNNNTGG 1 cut(s) 558
XapI RAATTY 2 cut(s) 143, 312
XceI RCATGY 1 cut(s) 221
XcmI CCANNNNNNNNNTGG 1 cut(s) 555
XmiI GTMKAC 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.