Rmu_sc0000730.1_g000004

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000730.1
N/A
Physical Location & Seq
Reverse (-)
23068 .. 23517
450 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 450 bp
atgaatggtaaaccggtgctaaaagctggggtgcagtggaagattggtgatgaaaaacatgtgaacacatggaaggattggtggattcctgatcgtccacaatttttaattcgcaggcatataagcacaaagtttgaaacggtggcagacttgatcaatgaggacactagagcttgggtatctttgtccaatggggagcctttcaaggaattgtggaaaagactttggaaggccaaagtacttgacaatgtacacatttgcatttggagagcttgtaacaactttttgcgtacaagagagaggttacttacaaaaggctataatggagacactagctgcaggctttgctctcaccgacttgaagactctgctcacctattttgtaagtgccctattgctatgcacatattgtttgttcccatttcaatttacagaatagtcttctactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

149

Amino Acids

17.71

Weight (kDa)

9.48

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 57 - 135 6.5e-15 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 3 cut(s) 240, 252, 292
AflIII ACRYGT 1 cut(s) 58
AgeI ACCGGT 1 cut(s) 13
AgsI TTSAA 4 cut(s) 137, 205, 362, 426
AluBI AGCT 4 cut(s) 26, 173, 272, 336
AluI AGCT 4 cut(s) 26, 173, 272, 336
Alw26I GTCTC 1 cut(s) 321
AoxI GGCC 1 cut(s) 231
ApeKI GCWGC 1 cut(s) 336
AsiGI ACCGGT 1 cut(s) 13
AsuHPI GGTGA 3 cut(s) 59, 344, 365
BaeGI GKGCMC 1 cut(s) 392
BbsI GAAGAC 2 cut(s) 369, 433
BbvI GCAGC 1 cut(s) 323
BclI TGATCA 1 cut(s) 153
BcoDI GTCTC 1 cut(s) 321
BfaI CTAG 2 cut(s) 168, 333
BfmI CTRYAG 1 cut(s) 337
BisI GCNGC 1 cut(s) 337
BlsI GCNGC 1 cut(s) 338
BmcAI AGTACT 1 cut(s) 240
BmiI GGNNCC 1 cut(s) 198
BpiI GAAGAC 2 cut(s) 369, 433
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bse118I RCCGGY 1 cut(s) 13
BseSI GKGCMC 1 cut(s) 392
BseXI GCAGC 1 cut(s) 323
BseYI CCCAGC 1 cut(s) 26
BsgI GTGCAG 1 cut(s) 53
BshFI GGCC 1 cut(s) 233
BshTI ACCGGT 1 cut(s) 13
BsiSI CCGG 1 cut(s) 14
BsmAI GTCTC 1 cut(s) 321
BsnI GGCC 1 cut(s) 233
Bsp1286I GDGCHC 1 cut(s) 392
Bsp1407I TGTACA 1 cut(s) 250
Bsp143I GATC 2 cut(s) 91, 153
BspANI GGCC 1 cut(s) 233
BspLI GGNNCC 1 cut(s) 198
BspMAI CTGCAG 1 cut(s) 341
BsrFI RCCGGY 1 cut(s) 13
BsrGI TGTACA 1 cut(s) 250
BssAI RCCGGY 1 cut(s) 13
BssMI GATC 2 cut(s) 91, 153
Bst4CI ACNGT 1 cut(s) 142
BstAPI GCANNNNNTGC 1 cut(s) 345
BstAUI TGTACA 1 cut(s) 250
BstC8I GCNNGC 2 cut(s) 116, 341
BstKTI GATC 2 cut(s) 94, 156
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 2 cut(s) 91, 153
BstMWI GCNNNNNNNGC 1 cut(s) 345
BstNSI RCATGY 1 cut(s) 62
BstSFI CTRYAG 1 cut(s) 337
BstSLI GKGCMC 1 cut(s) 392
BstV1I GCAGC 1 cut(s) 323
BstV2I GAAGAC 2 cut(s) 369, 433
BsuRI GGCC 1 cut(s) 233
BtsI GCAGTG 1 cut(s) 41
BtsIMutI CAGTG 1 cut(s) 41
Cac8I GCNNGC 2 cut(s) 116, 341
Cfr10I RCCGGY 1 cut(s) 13
Csp6I GTAC 3 cut(s) 239, 251, 291
CspAI ACCGGT 1 cut(s) 13
CviAII CATG 2 cut(s) 59, 69
CviJI RGCY 8 cut(s) 26, 173, 199, 233, 272, 318, 336, 343
CviKI_1 RGCY 8 cut(s) 26, 173, 199, 233, 272, 318, 336, 343
CviQI GTAC 3 cut(s) 239, 251, 291
DpnI GATC 2 cut(s) 93, 155
DpnII GATC 2 cut(s) 91, 153
FaeI CATG 2 cut(s) 62, 72
FaiI YATR 7 cut(s) 60, 70, 120, 122, 321, 401, 407
FatI CATG 2 cut(s) 58, 68
FbaI TGATCA 1 cut(s) 153
Fnu4HI GCNGC 1 cut(s) 337
Fsp4HI GCNGC 1 cut(s) 337
FspBI CTAG 2 cut(s) 168, 333
GluI GCNGC 1 cut(s) 337
GsaI CCCAGC 1 cut(s) 30
HaeIII GGCC 1 cut(s) 233
HapII CCGG 1 cut(s) 14
Hin1II CATG 2 cut(s) 62, 72
HinfI GANTC 2 cut(s) 85, 365
HpaII CCGG 1 cut(s) 14
HphI GGTGA 3 cut(s) 59, 344, 365
Hpy166II GTNNAC 4 cut(s) 11, 64, 98, 253
Hpy188III TCNNGA 1 cut(s) 89
Hpy8I GTNNAC 4 cut(s) 11, 64, 98, 253
HpyAV CCTTC 2 cut(s) 67, 223
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4V TGCA 4 cut(s) 34, 261, 339, 403
HpyF10VI GCNNNNNNNGC 1 cut(s) 345
Hsp92II CATG 2 cut(s) 62, 72
Ksp22I TGATCA 1 cut(s) 153
Kzo9I GATC 2 cut(s) 91, 153
LmnI GCTCC 1 cut(s) 196
LpnPI CCDG 5 cut(s) 12, 27, 100, 102, 325
Lsp1109I GCAGC 1 cut(s) 323
MaeI CTAG 2 cut(s) 168, 333
MaeIII GTNAC 2 cut(s) 275, 303
MalI GATC 2 cut(s) 93, 155
MboI GATC 2 cut(s) 91, 153
MboII GAAGA 3 cut(s) 52, 374, 433
MhlI GDGCHC 1 cut(s) 392
MluCI AATT 4 cut(s) 101, 108, 209, 426
MlyI GAGTC 1 cut(s) 359
MnlI CCTC 2 cut(s) 154, 294
MseI TTAA 1 cut(s) 107
MspI CCGG 1 cut(s) 14
MwoI GCNNNNNNNGC 1 cut(s) 345
NdeII GATC 2 cut(s) 91, 153
NlaIII CATG 2 cut(s) 62, 72
NlaIV GGNNCC 1 cut(s) 198
NspI RCATGY 1 cut(s) 62
PciI ACATGT 1 cut(s) 58
PfeI GAWTC 1 cut(s) 85
PinAI ACCGGT 1 cut(s) 13
PkrI GCNGC 1 cut(s) 338
PleI GAGTC 1 cut(s) 359
PpsI GAGTC 1 cut(s) 359
PscI ACATGT 1 cut(s) 58
PspFI CCCAGC 1 cut(s) 26
PspN4I GGNNCC 1 cut(s) 198
PstI CTGCAG 1 cut(s) 341
RsaI GTAC 3 cut(s) 240, 252, 292
RsaNI GTAC 3 cut(s) 239, 251, 291
SaqAI TTAA 1 cut(s) 107
SatI GCNGC 1 cut(s) 337
Sau3AI GATC 2 cut(s) 91, 153
ScaI AGTACT 1 cut(s) 240
SchI GAGTC 1 cut(s) 359
SduI GDGCHC 1 cut(s) 392
SetI ASST 6 cut(s) 28, 175, 274, 305, 338, 378
SfcI CTRYAG 1 cut(s) 337
Sse9I AATT 4 cut(s) 101, 108, 209, 426
SspMI CTAG 2 cut(s) 168, 333
TaaI ACNGT 1 cut(s) 142
TasI AATT 4 cut(s) 101, 108, 209, 426
TatI WGTACW 2 cut(s) 238, 250
TfiI GAWTC 1 cut(s) 85
Tru1I TTAA 1 cut(s) 107
Tru9I TTAA 1 cut(s) 107
TscAI CASTG 1 cut(s) 41
TseI GCWGC 1 cut(s) 336
TspDTI ATGAA 2 cut(s) 17, 66
TspRI CASTG 1 cut(s) 41
XceI RCATGY 1 cut(s) 62
XspI CTAG 2 cut(s) 168, 333
ZrmI AGTACT 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.