Rmu_sc0000776.1_g000035

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000776.1
N/A
Physical Location & Seq
Forward (+)
161884 .. 165538
3655 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 861 bp
atggcatcgtggcatcgtttcaagcgaccgacacgtctttctaagcccccttcaatcagttacattctagcgggcctgatttcagggcctggggggcaatgtttgagcccaaaagcaattttggcagaatcctttagtgggttcgagccagcgggccgatacctgcggctcaaaaataaggctacgcgggtttggtttatagtttcgctcatcccggttaagcggcgagataaaggacctctctcaaatcaatcaatcccagcgattacaaagcctcccaggagcgaaccttcaaccttgttgaaacccggtgaccgcccgccagtcctagtctcctcgcgagccgactgtcagcctcaccagatcaacctggcgaccaaaccacaaccaaagtccttatccgtaaggcaaacgtcctttcccggaaggcaagaaaagaaccctgtgacggggttggtgctctcctcgtccacagcgcttgaagaagaagtcaggtcacgggactaccccaacgactgcaccccgcggctcgatactgtggtggaatcttgtgaagttagtggttgctacaaggttgcattggggccaagtgttgcaagagaaagctcgagttccagacaccctgtttgttataatgcttatggagtgttcaaggacaaggacatgtatgagagaattttcggggatgaggagaagctcaagaatttcttgaggtggagagttcaggtccttgaaaagggtatcaacatcttgcattttaatccaggagggatcaagtccatcatccaagtcactgatctcaaggacatgcccaaaagagagctcagggttgcttcaaataagatcatttcgctgttttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

286

Amino Acids

31.9

Weight (kDa)

9.88

Isoelectric Point (pI)

54.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
AasI GACNNNNNNGTC 1 cut(s) 33
Acc36I ACCTGC 1 cut(s) 171
AccII CGCG 3 cut(s) 187, 340, 526
AciI CCGC 9 cut(s) 71, 152, 166, 187, 223, 316, 320, 524, 526
AclWI GGATC 1 cut(s) 779
AcsI RAATTY 2 cut(s) 675, 703
AfeI AGCGCT 1 cut(s) 477
AfiI CCNNNNNNNGG 4 cut(s) 138, 448, 449, 736
AflIII ACRYGT 2 cut(s) 32, 663
AgsI TTSAA 8 cut(s) 22, 54, 294, 304, 482, 652, 734, 837
AjiI CACGTC 1 cut(s) 35
AjnI CCWGG 4 cut(s) 88, 278, 369, 763
AluBI AGCT 3 cut(s) 606, 697, 823
AluI AGCT 3 cut(s) 606, 697, 823
Alw21I GWGCWC 2 cut(s) 462, 825
Alw26I GTCTC 1 cut(s) 337
AlwI GGATC 1 cut(s) 779
AlwNI CAGNNNCTG 1 cut(s) 89
Ama87I CYCGRG 1 cut(s) 607
Aor51HI AGCGCT 1 cut(s) 477
AoxI GGCC 4 cut(s) 73, 86, 154, 584
ApoI RAATTY 2 cut(s) 675, 703
ArsI GACNNNNNNTTYG 2 cut(s) 609, 641
AspLEI GCGC 1 cut(s) 478
AspS9I GGNCC 6 cut(s) 73, 86, 154, 236, 584, 727
AsuC2I CCSGG 3 cut(s) 215, 309, 423
AsuHPI GGTGA 2 cut(s) 323, 350
AvaI CYCGRG 1 cut(s) 607
AvaII GGWCC 2 cut(s) 236, 727
BaeI ACNNNNGTAYC 2 cut(s) 525, 558
BanII GRGCYC 2 cut(s) 110, 825
Bbv12I GWGCWC 2 cut(s) 462, 825
BccI CCATC 1 cut(s) 788
BciT130I CCWGG 4 cut(s) 90, 280, 371, 765
BcnI CCSGG 3 cut(s) 215, 309, 423
BcoDI GTCTC 1 cut(s) 337
BfaI CTAG 2 cut(s) 68, 329
BfoI RGCGCY 1 cut(s) 479
BfuAI ACCTGC 1 cut(s) 171
BglI GCCNNNNNGGC 1 cut(s) 94
BisI GCNGC 3 cut(s) 167, 224, 527
BlsI GCNGC 3 cut(s) 168, 225, 528
Bme1390I CCNGG 7 cut(s) 90, 215, 280, 309, 371, 423, 765
Bme18I GGWCC 2 cut(s) 236, 727
BmeT110I CYCGRG 1 cut(s) 607
BmgBI CACGTC 1 cut(s) 35
BmgT120I GGNCC 6 cut(s) 73, 86, 154, 236, 584, 727
BmiI GGNNCC 1 cut(s) 585
BmrFI CCNGG 7 cut(s) 90, 215, 280, 309, 371, 423, 765
BmsI GCATC 2 cut(s) 14, 22
Bpu10I CCTNAGC 1 cut(s) 824
BpuEI CTTGAG 3 cut(s) 683, 730, 785
BpuMI CCSGG 3 cut(s) 215, 309, 423
BsaJI CCNNGG 3 cut(s) 89, 278, 524
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 448, 449, 736
Bse1I ACTGG 1 cut(s) 323
Bse3DI GCAATG 1 cut(s) 104
BseBI CCWGG 4 cut(s) 90, 280, 371, 765
BseDI CCNNGG 3 cut(s) 89, 278, 524
BseGI GGATG 3 cut(s) 210, 691, 783
BseLI CCNNNNNNNGG 4 cut(s) 138, 448, 449, 736
BseMI GCAATG 1 cut(s) 104
BseMII CTCAG 1 cut(s) 838
BseNI ACTGG 1 cut(s) 323
BseRI GAGGAG 3 cut(s) 325, 454, 704
BseYI CCCAGC 1 cut(s) 259
BsgI GTGCAG 1 cut(s) 502
Bsh1236I CGCG 3 cut(s) 187, 340, 526
Bsh1285I CGRYCG 1 cut(s) 29
BshFI GGCC 4 cut(s) 75, 88, 156, 586
BsiEI CGRYCG 1 cut(s) 29
BsiHKAI GWGCWC 2 cut(s) 462, 825
BsiHKCI CYCGRG 1 cut(s) 607
BsiSI CCGG 3 cut(s) 215, 309, 423
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 4 cut(s) 138, 448, 449, 736
BsmAI GTCTC 1 cut(s) 337
BsmFI GGGAC 1 cut(s) 515
BsnI GGCC 4 cut(s) 75, 88, 156, 586
BsoBI CYCGRG 1 cut(s) 607
Bsp1286I GDGCHC 3 cut(s) 110, 462, 825
Bsp143I GATC 4 cut(s) 363, 771, 796, 843
Bsp68I TCGCGA 1 cut(s) 340
BspACI CCGC 9 cut(s) 71, 152, 166, 187, 223, 316, 320, 524, 526
BspANI GGCC 4 cut(s) 75, 88, 156, 586
BspCNI CTCAG 1 cut(s) 837
BspFNI CGCG 3 cut(s) 187, 340, 526
BspLI GGNNCC 1 cut(s) 585
BspMI ACCTGC 1 cut(s) 171
BspPI GGATC 1 cut(s) 779
BsrDI GCAATG 1 cut(s) 104
BsrI ACTGG 1 cut(s) 323
BssECI CCNNGG 3 cut(s) 89, 278, 524
BssMI GATC 4 cut(s) 363, 771, 796, 843
Bst2UI CCWGG 4 cut(s) 90, 280, 371, 765
Bst4CI ACNGT 2 cut(s) 350, 538
BstC8I GCNNGC 5 cut(s) 73, 150, 154, 320, 342
BstDEI CTNAG 2 cut(s) 42, 824
BstDSI CCRYGG 1 cut(s) 524
BstEII GGTNACC 1 cut(s) 311
BstENI CCTNNNNNAGG 1 cut(s) 734
BstF5I GGATG 3 cut(s) 210, 691, 783
BstFNI CGCG 3 cut(s) 187, 340, 526
BstH2I RGCGCY 1 cut(s) 479
BstHHI GCGC 1 cut(s) 478
BstKTI GATC 4 cut(s) 366, 774, 799, 846
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 4 cut(s) 363, 771, 796, 843
BstMCI CGRYCG 1 cut(s) 29
BstMWI GCNNNNNNNGC 2 cut(s) 94, 122
BstNI CCWGG 4 cut(s) 90, 280, 371, 765
BstNSI RCATGY 2 cut(s) 667, 811
BstPI GGTNACC 1 cut(s) 311
BstSCI CCNGG 7 cut(s) 88, 213, 278, 307, 369, 421, 763
BstUI CGCG 3 cut(s) 187, 340, 526
BsuRI GGCC 4 cut(s) 75, 88, 156, 586
BtgI CCRYGG 1 cut(s) 524
BtrI CACGTC 1 cut(s) 35
BtsCI GGATG 3 cut(s) 210, 691, 783
BtsIMutI CAGTG 1 cut(s) 792
BtuMI TCGCGA 1 cut(s) 340
BveI ACCTGC 1 cut(s) 171
Cac8I GCNNGC 5 cut(s) 73, 150, 154, 320, 342
CaiI CAGNNNCTG 1 cut(s) 89
CfoI GCGC 1 cut(s) 478
Cfr13I GGNCC 6 cut(s) 73, 86, 154, 236, 584, 727
Cfr42I CCGCGG 1 cut(s) 527
CspCI CAANNNNNGTGG 2 cut(s) 460, 495
CviAII CATG 2 cut(s) 664, 808
DdeI CTNAG 2 cut(s) 42, 824
DpnI GATC 4 cut(s) 365, 773, 798, 845
DpnII GATC 4 cut(s) 363, 771, 796, 843
DrdI GACNNNNNNGTC 1 cut(s) 33
DseDI GACNNNNNNGTC 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 823
Eco24I GRGCYC 2 cut(s) 110, 825
Eco47I GGWCC 2 cut(s) 236, 727
Eco47III AGCGCT 1 cut(s) 477
Eco53kI GAGCTC 1 cut(s) 823
Eco88I CYCGRG 1 cut(s) 607
Eco91I GGTNACC 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 823
EcoNI CCTNNNNNAGG 1 cut(s) 734
EcoO109I RGGNCCY 3 cut(s) 86, 236, 727
EcoO65I GGTNACC 1 cut(s) 311
EcoRII CCWGG 4 cut(s) 88, 278, 369, 763
EcoT38I GRGCYC 2 cut(s) 110, 825
FaeI CATG 2 cut(s) 667, 811
FaiI YATR 6 cut(s) 200, 633, 642, 665, 669, 809
FalI AAGNNNNNCTT 2 cut(s) 692, 724
FaqI GGGAC 1 cut(s) 515
FatI CATG 2 cut(s) 663, 807
FauI CCCGC 5 cut(s) 64, 145, 180, 327, 531
Fnu4HI GCNGC 3 cut(s) 167, 224, 527
FokI GGATG 3 cut(s) 197, 698, 770
FriOI GRGCYC 2 cut(s) 110, 825
Fsp4HI GCNGC 3 cut(s) 167, 224, 527
FspBI CTAG 2 cut(s) 68, 329
GlaI GCGC 1 cut(s) 477
GluI GCNGC 3 cut(s) 167, 224, 527
GsaI CCCAGC 1 cut(s) 263
HaeII RGCGCY 1 cut(s) 479
HaeIII GGCC 4 cut(s) 75, 88, 156, 586
HapII CCGG 3 cut(s) 215, 309, 423
HhaI GCGC 1 cut(s) 478
Hin1II CATG 2 cut(s) 667, 811
Hin6I GCGC 1 cut(s) 476
HinP1I GCGC 1 cut(s) 476
HinfI GANTC 2 cut(s) 128, 545
HpaII CCGG 3 cut(s) 215, 309, 423
HphI GGTGA 2 cut(s) 323, 350
Hpy166II GTNNAC 1 cut(s) 471
Hpy188III TCNNGA 4 cut(s) 339, 615, 700, 709
Hpy8I GTNNAC 1 cut(s) 471
HpyAV CCTTC 3 cut(s) 60, 300, 420
HpyCH4III ACNGT 2 cut(s) 350, 538
HpyCH4IV ACGT 2 cut(s) 34, 413
HpyCH4V TGCA 4 cut(s) 519, 578, 596, 754
HpyF10VI GCNNNNNNNGC 2 cut(s) 94, 122
HpyF3I CTNAG 2 cut(s) 42, 824
HpySE526I ACGT 2 cut(s) 34, 413
Hsp92II CATG 2 cut(s) 667, 811
HspAI GCGC 1 cut(s) 476
KspI CCGCGG 1 cut(s) 527
Kzo9I GATC 4 cut(s) 363, 771, 796, 843
LmnI GCTCC 1 cut(s) 282
LweI GCATC 2 cut(s) 14, 22
MaeI CTAG 2 cut(s) 68, 329
MaeII ACGT 2 cut(s) 34, 413
MaeIII GTNAC 5 cut(s) 59, 311, 445, 495, 790
MalI GATC 4 cut(s) 365, 773, 798, 845
MboI GATC 4 cut(s) 363, 771, 796, 843
MboII GAAGA 2 cut(s) 494, 497
MhlI GDGCHC 3 cut(s) 110, 462, 825
MluCI AATT 3 cut(s) 117, 675, 703
MnlI CCTC 8 cut(s) 249, 285, 346, 366, 475, 682, 705, 761
MseI TTAA 2 cut(s) 219, 759
MspA1I CMGCKG 2 cut(s) 152, 526
MspI CCGG 3 cut(s) 215, 309, 423
MspR9I CCNGG 7 cut(s) 90, 215, 280, 309, 371, 423, 765
MvaI CCWGG 4 cut(s) 90, 280, 371, 765
MvnI CGCG 3 cut(s) 187, 340, 526
MwoI GCNNNNNNNGC 2 cut(s) 94, 122
NciI CCSGG 3 cut(s) 215, 309, 423
NdeII GATC 4 cut(s) 363, 771, 796, 843
NlaIII CATG 2 cut(s) 667, 811
NlaIV GGNNCC 1 cut(s) 585
NmuCI GTSAC 4 cut(s) 311, 445, 495, 790
NruI TCGCGA 1 cut(s) 340
NspI RCATGY 2 cut(s) 667, 811
PaeR7I CTCGAG 1 cut(s) 607
PciI ACATGT 1 cut(s) 663
PcsI WCGNNNNNNNCGW 1 cut(s) 22
PfeI GAWTC 2 cut(s) 128, 545
PfoI TCCNGGA 2 cut(s) 421, 763
PkrI GCNGC 3 cut(s) 168, 225, 528
PpuMI RGGWCCY 2 cut(s) 236, 727
PscI ACATGT 1 cut(s) 663
PsiI TTATAA 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 825
Psp5II RGGWCCY 2 cut(s) 236, 727
Psp6I CCWGG 4 cut(s) 88, 278, 369, 763
PspEI GGTNACC 1 cut(s) 311
PspFI CCCAGC 1 cut(s) 259
PspGI CCWGG 4 cut(s) 88, 278, 369, 763
PspN4I GGNNCC 1 cut(s) 585
PspPI GGNCC 6 cut(s) 73, 86, 154, 236, 584, 727
PspPPI RGGWCCY 2 cut(s) 236, 727
PspXI VCTCGAGB 1 cut(s) 607
PstNI CAGNNNCTG 1 cut(s) 89
RruI TCGCGA 1 cut(s) 340
SacI GAGCTC 1 cut(s) 825
SacII CCGCGG 1 cut(s) 527
SaqAI TTAA 2 cut(s) 219, 759
SatI GCNGC 3 cut(s) 167, 224, 527
Sau3AI GATC 4 cut(s) 363, 771, 796, 843
Sau96I GGNCC 6 cut(s) 73, 86, 154, 236, 584, 727
ScrFI CCNGG 7 cut(s) 90, 215, 280, 309, 371, 423, 765
SduI GDGCHC 3 cut(s) 110, 462, 825
SfaNI GCATC 2 cut(s) 14, 22
Sfr274I CTCGAG 1 cut(s) 607
Sfr303I CCGCGG 1 cut(s) 527
SgrBI CCGCGG 1 cut(s) 527
SinI GGWCC 2 cut(s) 236, 727
SlaI CTCGAG 1 cut(s) 607
SmlI CTYRAG 4 cut(s) 607, 698, 709, 800
SmoI CTYRAG 4 cut(s) 607, 698, 709, 800
Sse9I AATT 3 cut(s) 117, 675, 703
SsiI CCGC 9 cut(s) 71, 152, 166, 187, 223, 316, 320, 524, 526
SspMI CTAG 2 cut(s) 68, 329
SstI GAGCTC 1 cut(s) 825
StyD4I CCNGG 7 cut(s) 88, 213, 278, 307, 369, 421, 763
TaaI ACNGT 2 cut(s) 350, 538
TaiI ACGT 2 cut(s) 37, 416
TaqI TCGA 3 cut(s) 144, 531, 608
TaqII GACCGA 1 cut(s) 43
TasI AATT 3 cut(s) 117, 675, 703
TauI GCSGC 3 cut(s) 169, 226, 529
TfiI GAWTC 2 cut(s) 128, 545
Tru1I TTAA 2 cut(s) 219, 759
Tru9I TTAA 2 cut(s) 219, 759
TscAI CASTG 1 cut(s) 799
TseFI GTSAC 4 cut(s) 311, 445, 495, 790
Tsp45I GTSAC 4 cut(s) 311, 445, 495, 790
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 1 cut(s) 799
VpaK11BI GGWCC 2 cut(s) 236, 727
XagI CCTNNNNNAGG 1 cut(s) 734
XapI RAATTY 2 cut(s) 675, 703
XceI RCATGY 2 cut(s) 667, 811
XhoI CTCGAG 1 cut(s) 607
XspI CTAG 2 cut(s) 68, 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.