Rmu_sc0000810.1_g000019

ribonuclease H protein At1g65750-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000810.1
N/A
Physical Location & Seq
Reverse (-)
86323 .. 86793
471 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
atggacgtggatgtacttagctggaactgtagaggaatctgtaacgatgggacatcaagagctctgaaggatcttatttcacaaaataggcctcagattgtgttcctgtgtgaaacaaaaataagccgaaaggaggactttgacccgttgcagagagcgctgggattcgtgaatgtggagatggtgctcagtgagggtctgtccggcggtcttggactgttatggaatgatgaggtaaatctgagaatgggtactacgtcagcccatcatatggatgcggtgattgttggggaagcgggtactccctcgtggagagtgactggcttctattggtacgccagaacaggggagagggataggtcgtggaagctactgcgtgatcttgctgacctagattcgttatcgtgggttgtagtgggagactttaatgaaatccttaacaatggggagaagattgatggagttgtgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

156

Amino Acids

17.48

Weight (kDa)

4.78

Isoelectric Point (pI)

23.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 6 - 149 2e-13 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 271
AciI CCGC 3 cut(s) 207, 278, 296
AclWI GGATC 1 cut(s) 78
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 4 cut(s) 15, 253, 301, 335
AfeI AGCGCT 1 cut(s) 159
AfiI CCNNNNNNNGG 3 cut(s) 133, 271, 345
AjiI CACGTC 1 cut(s) 7
AluBI AGCT 3 cut(s) 21, 62, 370
AluI AGCT 3 cut(s) 21, 62, 370
Alw21I GWGCWC 2 cut(s) 64, 189
Alw26I GTCTC 1 cut(s) 414
AlwI GGATC 1 cut(s) 78
Aor51HI AGCGCT 1 cut(s) 159
AoxI GGCC 1 cut(s) 89
AspLEI GCGC 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 292
BanII GRGCYC 1 cut(s) 64
BauI CACGAG 1 cut(s) 307
Bbv12I GWGCWC 2 cut(s) 64, 189
BccI CCATC 4 cut(s) 41, 175, 273, 452
BcoDI GTCTC 1 cut(s) 414
BfaI CTAG 1 cut(s) 392
BfmI CTRYAG 1 cut(s) 28
BfoI RGCGCY 1 cut(s) 161
BmgBI CACGTC 1 cut(s) 7
BmsI GCATC 1 cut(s) 265
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 271, 345
Bse1I ACTGG 1 cut(s) 325
BseGI GGATG 2 cut(s) 16, 280
BseLI CCNNNNNNNGG 3 cut(s) 133, 271, 345
BseMII CTCAG 3 cut(s) 107, 202, 233
BseNI ACTGG 1 cut(s) 325
BseYI CCCAGC 1 cut(s) 160
BshFI GGCC 1 cut(s) 91
BsiHKAI GWGCWC 2 cut(s) 64, 189
BsiSI CCGG 1 cut(s) 204
BslFI GGGAC 1 cut(s) 64
BslI CCNNNNNNNGG 3 cut(s) 133, 271, 345
BsmAI GTCTC 1 cut(s) 414
BsmFI GGGAC 1 cut(s) 64
BsnI GGCC 1 cut(s) 91
Bsp1286I GDGCHC 2 cut(s) 64, 189
Bsp143I GATC 2 cut(s) 70, 379
BspACI CCGC 3 cut(s) 207, 278, 296
BspANI GGCC 1 cut(s) 91
BspCNI CTCAG 3 cut(s) 106, 201, 234
BspPI GGATC 1 cut(s) 78
BsrI ACTGG 1 cut(s) 325
BssMI GATC 2 cut(s) 70, 379
BssSI CACGAG 1 cut(s) 307
Bst2BI CACGAG 1 cut(s) 307
Bst4CI ACNGT 2 cut(s) 29, 219
BstDEI CTNAG 4 cut(s) 17, 93, 188, 242
BstF5I GGATG 2 cut(s) 16, 280
BstH2I RGCGCY 1 cut(s) 161
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 2 cut(s) 73, 382
BstMAI GTCTC 1 cut(s) 414
BstMBI GATC 2 cut(s) 70, 379
BstMWI GCNNNNNNNGC 1 cut(s) 157
BstSFI CTRYAG 1 cut(s) 28
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BsuRI GGCC 1 cut(s) 91
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 2 cut(s) 16, 280
BtsIMutI CAGTG 1 cut(s) 196
CfoI GCGC 1 cut(s) 160
Csp6I GTAC 4 cut(s) 14, 252, 300, 334
CviJI RGCY 7 cut(s) 21, 62, 91, 126, 263, 324, 370
CviKI_1 RGCY 7 cut(s) 21, 62, 91, 126, 263, 324, 370
CviQI GTAC 4 cut(s) 14, 252, 300, 334
DdeI CTNAG 4 cut(s) 17, 93, 188, 242
DpnI GATC 2 cut(s) 72, 381
DpnII GATC 2 cut(s) 70, 379
Ecl136II GAGCTC 1 cut(s) 62
Eco147I AGGCCT 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 64
Eco47III AGCGCT 1 cut(s) 159
Eco53kI GAGCTC 1 cut(s) 62
Eco57I CTGAAG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 62
EcoT38I GRGCYC 1 cut(s) 64
FaiI YATR 3 cut(s) 223, 270, 272
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FaqI GGGAC 1 cut(s) 64
FauI CCCGC 1 cut(s) 289
FauNDI CATATG 1 cut(s) 270
FokI GGATG 2 cut(s) 23, 287
FriOI GRGCYC 1 cut(s) 64
FspBI CTAG 1 cut(s) 392
GlaI GCGC 1 cut(s) 159
GsaI CCCAGC 1 cut(s) 164
HaeII RGCGCY 1 cut(s) 161
HaeIII GGCC 1 cut(s) 91
HapII CCGG 1 cut(s) 204
HhaI GCGC 1 cut(s) 160
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HinfI GANTC 3 cut(s) 36, 165, 395
HpaII CCGG 1 cut(s) 204
HphI GGTGA 1 cut(s) 292
Hpy188I TCNGA 3 cut(s) 66, 96, 243
Hpy188III TCNNGA 2 cut(s) 57, 169
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 2 cut(s) 29, 219
HpyCH4IV ACGT 2 cut(s) 6, 257
HpyCH4V TGCA 1 cut(s) 151
HpyF10VI GCNNNNNNNGC 1 cut(s) 157
HpyF3I CTNAG 4 cut(s) 17, 93, 188, 242
HpySE526I ACGT 2 cut(s) 6, 257
HspAI GCGC 1 cut(s) 158
Kzo9I GATC 2 cut(s) 70, 379
LpnPI CCDG 7 cut(s) 7, 119, 146, 217, 306, 330, 352
LweI GCATC 1 cut(s) 265
MaeI CTAG 1 cut(s) 392
MaeII ACGT 2 cut(s) 6, 257
MaeIII GTNAC 2 cut(s) 41, 316
MalI GATC 2 cut(s) 72, 381
MboI GATC 2 cut(s) 70, 379
MboII GAAGA 1 cut(s) 463
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 2 cut(s) 64, 189
MnlI CCTC 7 cut(s) 26, 102, 127, 187, 226, 316, 345
MseI TTAA 2 cut(s) 426, 438
MslI CAYNNNNRTG 1 cut(s) 273
MspI CCGG 1 cut(s) 204
MwoI GCNNNNNNNGC 1 cut(s) 157
NdeI CATATG 1 cut(s) 270
NdeII GATC 2 cut(s) 70, 379
NmuCI GTSAC 1 cut(s) 316
PceI AGGCCT 1 cut(s) 91
PfeI GAWTC 3 cut(s) 36, 165, 395
PflMI CCANNNNNTGG 1 cut(s) 271
Psp124BI GAGCTC 1 cut(s) 64
PspFI CCCAGC 1 cut(s) 160
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 4 cut(s) 15, 253, 301, 335
RsaNI GTAC 4 cut(s) 14, 252, 300, 334
RseI CAYNNNNRTG 1 cut(s) 273
SacI GAGCTC 1 cut(s) 64
SaqAI TTAA 2 cut(s) 426, 438
Sau3AI GATC 2 cut(s) 70, 379
SduI GDGCHC 2 cut(s) 64, 189
SetI ASST 8 cut(s) 9, 23, 64, 237, 260, 362, 372, 393
SfaNI GCATC 1 cut(s) 265
SfcI CTRYAG 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 273
SseBI AGGCCT 1 cut(s) 91
SsiI CCGC 3 cut(s) 207, 278, 296
SspMI CTAG 1 cut(s) 392
SstI GAGCTC 1 cut(s) 64
StuI AGGCCT 1 cut(s) 91
TaaI ACNGT 2 cut(s) 29, 219
TaiI ACGT 2 cut(s) 9, 260
TatI WGTACW 1 cut(s) 13
TfiI GAWTC 3 cut(s) 36, 165, 395
Tru1I TTAA 2 cut(s) 426, 438
Tru9I TTAA 2 cut(s) 426, 438
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 1 cut(s) 316
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 1 cut(s) 444
TspRI CASTG 1 cut(s) 196
Van91I CCANNNNNTGG 1 cut(s) 271
XspI CTAG 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.