Rmu_sc0000827.1_g000023

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000827.1
N/A
Physical Location & Seq
Reverse (-)
107074 .. 108089
1016 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 345 bp
ctggtcagtaccgtcaccgatttgacagccgtcgacgccgccgtgaaggccatggagcttcaggtccaggccatcaaggacctggttcgtgacgaaaccctcgccatccccaaagcaaaaaaaactaatcgatgcatcgctgaagcagcaaaggaaattgaaggacatgtcaatttatgcgcccgtttatttccagatagctctcagcaaaacactggttctgggaccttcaagcttgacgaggatcctgcaccaccaccaacactgcctaaggagaaaaagggtcgttccacgccaccattgtggtttataagtgctgaagagctggattccttgtcaccgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

114

Amino Acids

12.31

Weight (kDa)

5.14

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 4 - 113 5e-14 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 311
AccI GTMKAC 1 cut(s) 33
AciI CCGC 1 cut(s) 39
AclWI GGATC 2 cut(s) 239, 252
AcuI CTGAAG 3 cut(s) 44, 162, 339
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 1 cut(s) 10
AflIII ACRYGT 1 cut(s) 166
AgsI TTSAA 2 cut(s) 161, 232
AjnI CCWGG 2 cut(s) 66, 81
AleI CACNNNNGTG 1 cut(s) 301
AluBI AGCT 4 cut(s) 58, 201, 235, 325
AluI AGCT 4 cut(s) 58, 201, 235, 325
AlwI GGATC 2 cut(s) 239, 252
AoxI GGCC 2 cut(s) 48, 69
ApeKI GCWGC 1 cut(s) 146
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 3 cut(s) 64, 79, 225
AsuHPI GGTGA 2 cut(s) 7, 330
AvaII GGWCC 3 cut(s) 64, 79, 225
AxyI CCTNAGG 1 cut(s) 270
BamHI GGATCC 1 cut(s) 244
BbvI GCAGC 1 cut(s) 158
BccI CCATC 2 cut(s) 80, 113
BceAI ACGGC 2 cut(s) 14, 26
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 2 cut(s) 68, 83
BglI GCCNNNNNGGC 1 cut(s) 47
BisI GCNGC 2 cut(s) 39, 147
BlsI GCNGC 2 cut(s) 40, 148
Bme1390I CCNGG 2 cut(s) 68, 83
Bme18I GGWCC 3 cut(s) 64, 79, 225
BmgT120I GGNCC 3 cut(s) 64, 79, 225
BmiI GGNNCC 2 cut(s) 226, 246
BmrFI CCNGG 2 cut(s) 68, 83
BmsI GCATC 2 cut(s) 122, 144
BoxI GACNNNNGTC 1 cut(s) 29
Bsa29I ATCGAT 1 cut(s) 130
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 51
Bse1I ACTGG 1 cut(s) 220
Bse21I CCTNAGG 1 cut(s) 270
BseBI CCWGG 2 cut(s) 68, 83
BseCI ATCGAT 1 cut(s) 130
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 1 cut(s) 105
BseMII CTCAG 1 cut(s) 218
BseNI ACTGG 1 cut(s) 220
BseXI GCAGC 1 cut(s) 158
BsgI GTGCAG 1 cut(s) 234
BshFI GGCC 2 cut(s) 50, 71
BshVI ATCGAT 1 cut(s) 130
BslFI GGGAC 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 2 cut(s) 50, 71
Bsp143I GATC 1 cut(s) 244
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 1 cut(s) 39
BspANI GGCC 2 cut(s) 50, 71
BspCNI CTCAG 1 cut(s) 217
BspDI ATCGAT 1 cut(s) 130
BspLI GGNNCC 2 cut(s) 226, 246
BspPI GGATC 2 cut(s) 239, 252
BspQI GCTCTTC 1 cut(s) 315
BsrI ACTGG 1 cut(s) 220
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 1 cut(s) 244
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 51
Bst2UI CCWGG 2 cut(s) 68, 83
Bst4CI ACNGT 2 cut(s) 13, 342
Bst6I CTCTTC 1 cut(s) 315
BstACI GRCGYC 1 cut(s) 36
BstDEI CTNAG 2 cut(s) 204, 270
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 1 cut(s) 105
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 1 cut(s) 247
BstMBI GATC 1 cut(s) 244
BstMWI GCNNNNNNNGC 3 cut(s) 35, 47, 146
BstNI CCWGG 2 cut(s) 68, 83
BstNSI RCATGY 1 cut(s) 170
BstPAI GACNNNNGTC 1 cut(s) 29
BstSCI CCNGG 2 cut(s) 66, 81
BstV1I GCAGC 1 cut(s) 158
BstX2I RGATCY 1 cut(s) 244
BstXI CCANNNNNNTGG 1 cut(s) 303
BstYI RGATCY 1 cut(s) 244
Bsu15I ATCGAT 1 cut(s) 130
Bsu36I CCTNAGG 1 cut(s) 270
BsuRI GGCC 2 cut(s) 50, 71
BsuTUI ATCGAT 1 cut(s) 130
BtgI CCRYGG 1 cut(s) 51
BtgZI GCGATG 1 cut(s) 121
BtsCI GGATG 1 cut(s) 105
BtsI GCAGTG 1 cut(s) 263
BtsIMutI CAGTG 2 cut(s) 213, 263
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 3 cut(s) 64, 79, 225
ClaI ATCGAT 1 cut(s) 130
CseI GACGC 1 cut(s) 44
CsiI ACCWGGT 1 cut(s) 81
Csp6I GTAC 1 cut(s) 9
CviAII CATG 2 cut(s) 52, 167
CviJI RGCY 7 cut(s) 29, 50, 58, 71, 201, 235, 325
CviKI_1 RGCY 7 cut(s) 29, 50, 58, 71, 201, 235, 325
CviQI GTAC 1 cut(s) 9
DdeI CTNAG 2 cut(s) 204, 270
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Eco130I CCWWGG 1 cut(s) 51
Eco47I GGWCC 3 cut(s) 64, 79, 225
Eco57I CTGAAG 3 cut(s) 44, 162, 339
Eco81I CCTNAGG 1 cut(s) 270
EcoO109I RGGNCCY 2 cut(s) 79, 225
EcoRII CCWGG 2 cut(s) 66, 81
EcoT14I CCWWGG 1 cut(s) 51
EcoT22I ATGCAT 1 cut(s) 137
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 2 cut(s) 55, 170
FaiI YATR 4 cut(s) 53, 168, 178, 311
FaqI GGGAC 1 cut(s) 238
FatI CATG 2 cut(s) 51, 166
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 2 cut(s) 39, 147
FokI GGATG 1 cut(s) 92
Fsp4HI GCNGC 2 cut(s) 39, 147
GlaI GCGC 1 cut(s) 181
GluI GCNGC 2 cut(s) 39, 147
HaeIII GGCC 2 cut(s) 50, 71
HgaI GACGC 1 cut(s) 44
HhaI GCGC 1 cut(s) 182
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 2 cut(s) 55, 170
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 1 cut(s) 329
HphI GGTGA 2 cut(s) 7, 330
Hpy166II GTNNAC 1 cut(s) 34
Hpy188III TCNNGA 2 cut(s) 89, 194
Hpy8I GTNNAC 1 cut(s) 34
Hpy99I CGWCG 2 cut(s) 35, 38
HpyAV CCTTC 3 cut(s) 40, 155, 238
HpyCH4III ACNGT 2 cut(s) 13, 342
HpyCH4V TGCA 2 cut(s) 135, 251
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 47, 146
HpyF3I CTNAG 2 cut(s) 204, 270
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 2 cut(s) 55, 170
HspAI GCGC 1 cut(s) 180
Kzo9I GATC 1 cut(s) 244
LguI GCTCTTC 1 cut(s) 315
LmnI GCTCC 1 cut(s) 55
Lsp1109I GCAGC 1 cut(s) 158
LweI GCATC 2 cut(s) 122, 144
MabI ACCWGGT 1 cut(s) 81
MaeIII GTNAC 3 cut(s) 13, 89, 336
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 1 cut(s) 332
MflI RGATCY 1 cut(s) 244
MluCI AATT 2 cut(s) 156, 172
MnlI CCTC 2 cut(s) 110, 235
Mph1103I ATGCAT 1 cut(s) 137
MslI CAYNNNNRTG 1 cut(s) 301
MspR9I CCNGG 2 cut(s) 68, 83
MvaI CCWGG 2 cut(s) 68, 83
MwoI GCNNNNNNNGC 3 cut(s) 35, 47, 146
NcoI CCATGG 1 cut(s) 51
NdeII GATC 1 cut(s) 244
NlaIII CATG 2 cut(s) 55, 170
NlaIV GGNNCC 2 cut(s) 226, 246
NmuCI GTSAC 3 cut(s) 13, 89, 336
NsiI ATGCAT 1 cut(s) 137
NspI RCATGY 1 cut(s) 170
OliI CACNNNNGTG 1 cut(s) 301
PciI ACATGT 1 cut(s) 166
PciSI GCTCTTC 1 cut(s) 315
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PfeI GAWTC 1 cut(s) 329
PkrI GCNGC 2 cut(s) 40, 148
PpuMI RGGWCCY 2 cut(s) 79, 225
PscI ACATGT 1 cut(s) 166
PshAI GACNNNNGTC 1 cut(s) 29
PsiI TTATAA 1 cut(s) 311
Psp5II RGGWCCY 2 cut(s) 79, 225
Psp6I CCWGG 2 cut(s) 66, 81
PspGI CCWGG 2 cut(s) 66, 81
PspN4I GGNNCC 2 cut(s) 226, 246
PspPI GGNCC 3 cut(s) 64, 79, 225
PspPPI RGGWCCY 2 cut(s) 79, 225
PsuI RGATCY 1 cut(s) 244
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
RseI CAYNNNNRTG 1 cut(s) 301
SalI GTCGAC 1 cut(s) 32
SapI GCTCTTC 1 cut(s) 315
SatI GCNGC 2 cut(s) 39, 147
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 3 cut(s) 64, 79, 225
ScrFI CCNGG 2 cut(s) 68, 83
SetI ASST 7 cut(s) 60, 66, 84, 203, 230, 237, 327
SexAI ACCWGGT 1 cut(s) 81
SfaNI GCATC 2 cut(s) 122, 144
SgrDI CGTCGACG 1 cut(s) 32
SinI GGWCC 3 cut(s) 64, 79, 225
SmiMI CAYNNNNRTG 1 cut(s) 301
Sse9I AATT 2 cut(s) 156, 172
SsiI CCGC 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 66, 81
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 2 cut(s) 13, 342
TaqI TCGA 2 cut(s) 33, 130
TasI AATT 2 cut(s) 156, 172
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 1 cut(s) 329
TscAI CASTG 2 cut(s) 220, 270
TseFI GTSAC 3 cut(s) 13, 89, 336
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 3 cut(s) 13, 89, 336
TspRI CASTG 2 cut(s) 220, 270
VpaK11BI GGWCC 3 cut(s) 64, 79, 225
XceI RCATGY 1 cut(s) 170
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 33
Zsp2I ATGCAT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.