Rmu_sc0000935.1_g000002

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000935.1
N/A
Physical Location & Seq
Forward (+)
3516 .. 5667
2152 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 513 bp
atgtcaggccctcagtgctgctcgaacccaccgaccctgaacccaaacagtggatccggccatgttcagaagcttggtggtctcgactcctatctcactggctctcctgactccaagcttgccattgttcttgcctctgacgcttttgggtatgatgctccaaacctgaggaagctcgcagataaagttgcagctgctggcttctttgttgtggtccctgacttcttttatggaaacccttatgtacctccagctgatggatcccatggcggtatacctacatggctgaagggtcatggaccggagaagggatttgaagatgttaaagtagttattgaagttctaaaaagtaaaggtgtttctgcaatcggtgctgcaggcttctgttggggagttgaacttgcaaagggtgactttatccaagctgctgttctggctcatccttcatttgtcactgtggatgatatcaagggtaagagttctctattcaataatcaagaaatatctgtctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

170

Amino Acids

17.68

Weight (kDa)

5.73

Isoelectric Point (pI)

22.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 29 - 152 3.3e-13 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 50, 257
AccI GTMKAC 1 cut(s) 274
AciI CCGC 1 cut(s) 270
AclWI GGATC 4 cut(s) 48, 61, 255, 268
AcoI YGGCCR 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 308
AfaI GTAC 1 cut(s) 246
AfiI CCNNNNNNNGG 3 cut(s) 50, 257, 308
AgsI TTSAA 4 cut(s) 317, 338, 398, 490
AjuI GAANNNNNNNTTGG 2 cut(s) 414, 446
AluBI AGCT 6 cut(s) 73, 118, 175, 194, 254, 425
AluI AGCT 6 cut(s) 73, 118, 175, 194, 254, 425
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 4 cut(s) 48, 61, 255, 268
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 2 cut(s) 7, 58
ApeKI GCWGC 5 cut(s) 18, 191, 194, 374, 425
AspS9I GGNCC 3 cut(s) 8, 214, 299
AsuHPI GGTGA 1 cut(s) 422
AvaII GGWCC 2 cut(s) 214, 299
AxyI CCTNAGG 1 cut(s) 167
BamHI GGATCC 2 cut(s) 53, 260
BbvI GCAGC 5 cut(s) 5, 181, 203, 361, 412
BccI CCATC 1 cut(s) 251
BcoDI GTCTC 1 cut(s) 86
BfmI CTRYAG 1 cut(s) 375
BisI GCNGC 5 cut(s) 19, 192, 195, 375, 426
BlsI GCNGC 5 cut(s) 20, 193, 196, 376, 427
Bme18I GGWCC 2 cut(s) 214, 299
BmgT120I GGNCC 3 cut(s) 8, 214, 299
BmiI GGNNCC 3 cut(s) 55, 216, 262
BmsI GCATC 1 cut(s) 145
BpmI CTGGAG 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 265
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 88, 118
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 257, 308
Bse1I ACTGG 1 cut(s) 103
Bse21I CCTNAGG 1 cut(s) 167
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 2 cut(s) 439, 466
BseLI CCNNNNNNNGG 3 cut(s) 50, 257, 308
BseMII CTCAG 2 cut(s) 26, 158
BseNI ACTGG 1 cut(s) 103
BseXI GCAGC 5 cut(s) 5, 181, 203, 361, 412
BshFI GGCC 2 cut(s) 9, 60
BsiSI CCGG 2 cut(s) 57, 302
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 3 cut(s) 50, 257, 308
BsmAI GTCTC 1 cut(s) 86
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 2 cut(s) 9, 60
Bso31I GGTCTC 1 cut(s) 86
Bsp143I GATC 2 cut(s) 53, 260
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 1 cut(s) 270
BspANI GGCC 2 cut(s) 9, 60
BspCNI CTCAG 2 cut(s) 25, 159
BspLI GGNNCC 3 cut(s) 55, 216, 262
BspMAI CTGCAG 1 cut(s) 379
BspPI GGATC 4 cut(s) 48, 61, 255, 268
BspTNI GGTCTC 1 cut(s) 86
BsrI ACTGG 1 cut(s) 103
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 2 cut(s) 53, 260
BssNAI GTATAC 1 cut(s) 275
BssT1I CCWWGG 1 cut(s) 265
Bst1107I GTATAC 1 cut(s) 275
Bst4CI ACNGT 2 cut(s) 50, 457
BstAPI GCANNNNNTGC 1 cut(s) 371
BstC8I GCNNGC 4 cut(s) 120, 177, 199, 379
BstDEI CTNAG 2 cut(s) 12, 167
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 2 cut(s) 439, 466
BstKTI GATC 2 cut(s) 56, 263
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 2 cut(s) 53, 260
BstMWI GCNNNNNNNGC 4 cut(s) 15, 140, 371, 434
BstSFI CTRYAG 1 cut(s) 375
BstV1I GCAGC 5 cut(s) 5, 181, 203, 361, 412
BstX2I RGATCY 2 cut(s) 53, 260
BstYI RGATCY 2 cut(s) 53, 260
BstZ17I GTATAC 1 cut(s) 275
Bsu36I CCTNAGG 1 cut(s) 167
BsuRI GGCC 2 cut(s) 9, 60
BtgI CCRYGG 1 cut(s) 265
BtsCI GGATG 2 cut(s) 439, 466
BtsIMutI CAGTG 4 cut(s) 20, 55, 96, 453
Cac8I GCNNGC 4 cut(s) 120, 177, 199, 379
CaiI CAGNNNCTG 1 cut(s) 197
Cfr13I GGNCC 3 cut(s) 8, 214, 299
CseI GACGC 1 cut(s) 149
Csp6I GTAC 1 cut(s) 245
CviAII CATG 4 cut(s) 62, 266, 282, 296
CviQI GTAC 1 cut(s) 245
DdeI CTNAG 2 cut(s) 12, 167
DpnI GATC 2 cut(s) 55, 262
DpnII GATC 2 cut(s) 53, 260
EaeI YGGCCR 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 265
Eco31I GGTCTC 1 cut(s) 86
Eco32I GATATC 1 cut(s) 466
Eco47I GGWCC 2 cut(s) 214, 299
Eco57I CTGAAG 1 cut(s) 308
Eco81I CCTNAGG 1 cut(s) 167
EcoO109I RGGNCCY 1 cut(s) 8
EcoRV GATATC 1 cut(s) 466
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 4 cut(s) 65, 269, 285, 299
FaiI YATR 8 cut(s) 63, 153, 231, 243, 267, 275, 283, 297
FalI AAGNNNNNCTT 2 cut(s) 398, 430
FaqI GGGAC 1 cut(s) 200
FatI CATG 4 cut(s) 61, 265, 281, 295
FblI GTMKAC 1 cut(s) 274
Fnu4HI GCNGC 5 cut(s) 19, 192, 195, 375, 426
FokI GGATG 2 cut(s) 426, 473
Fsp4HI GCNGC 5 cut(s) 19, 192, 195, 375, 426
GluI GCNGC 5 cut(s) 19, 192, 195, 375, 426
GsuI CTGGAG 1 cut(s) 234
HaeIII GGCC 2 cut(s) 9, 60
HapII CCGG 2 cut(s) 57, 302
HgaI GACGC 1 cut(s) 149
Hin1II CATG 4 cut(s) 65, 269, 285, 299
HindIII AAGCTT 2 cut(s) 71, 116
HinfI GANTC 2 cut(s) 86, 110
HpaII CCGG 2 cut(s) 57, 302
HphI GGTGA 1 cut(s) 422
Hpy166II GTNNAC 1 cut(s) 275
Hpy188I TCNGA 3 cut(s) 69, 139, 512
Hpy188III TCNNGA 3 cut(s) 83, 107, 497
Hpy8I GTNNAC 1 cut(s) 275
HpyAV CCTTC 3 cut(s) 283, 301, 453
HpyCH4III ACNGT 2 cut(s) 50, 457
HpyCH4V TGCA 4 cut(s) 191, 365, 377, 404
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 140, 371, 434
HpyF3I CTNAG 2 cut(s) 12, 167
Hsp92II CATG 4 cut(s) 65, 269, 285, 299
Kzo9I GATC 2 cut(s) 53, 260
LmnI GCTCC 1 cut(s) 163
Lsp1109I GCAGC 5 cut(s) 5, 181, 203, 361, 412
LweI GCATC 1 cut(s) 145
MaeIII GTNAC 2 cut(s) 410, 451
MalI GATC 2 cut(s) 55, 262
MboI GATC 2 cut(s) 53, 260
MboII GAAGA 1 cut(s) 329
MflI RGATCY 2 cut(s) 53, 260
MlyI GAGTC 2 cut(s) 80, 104
MnlI CCTC 4 cut(s) 21, 145, 162, 258
MseI TTAA 1 cut(s) 324
MspA1I CMGCKG 2 cut(s) 194, 254
MspI CCGG 2 cut(s) 57, 302
MwoI GCNNNNNNNGC 4 cut(s) 15, 140, 371, 434
NcoI CCATGG 1 cut(s) 265
NdeII GATC 2 cut(s) 53, 260
NlaIII CATG 4 cut(s) 65, 269, 285, 299
NlaIV GGNNCC 3 cut(s) 55, 216, 262
NmuCI GTSAC 2 cut(s) 410, 451
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PflMI CCANNNNNTGG 2 cut(s) 50, 257
PkrI GCNGC 5 cut(s) 20, 193, 196, 376, 427
PleI GAGTC 2 cut(s) 80, 104
PpsI GAGTC 2 cut(s) 80, 104
PspN4I GGNNCC 3 cut(s) 55, 216, 262
PspPI GGNCC 3 cut(s) 8, 214, 299
PstI CTGCAG 1 cut(s) 379
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 2 cut(s) 53, 260
PvuII CAGCTG 2 cut(s) 194, 254
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 5 cut(s) 19, 192, 195, 375, 426
Sau3AI GATC 2 cut(s) 53, 260
Sau96I GGNCC 3 cut(s) 8, 214, 299
SchI GAGTC 2 cut(s) 80, 104
SfaNI GCATC 1 cut(s) 145
SfcI CTRYAG 1 cut(s) 375
SinI GGWCC 2 cut(s) 214, 299
SsiI CCGC 1 cut(s) 270
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 2 cut(s) 50, 457
TaqI TCGA 2 cut(s) 23, 84
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 4 cut(s) 20, 55, 103, 460
TseFI GTSAC 2 cut(s) 410, 451
TseI GCWGC 5 cut(s) 18, 191, 194, 374, 425
Tsp45I GTSAC 2 cut(s) 410, 451
TspDTI ATGAA 1 cut(s) 435
TspRI CASTG 4 cut(s) 20, 55, 103, 460
Van91I CCANNNNNTGG 2 cut(s) 50, 257
VpaK11BI GGWCC 2 cut(s) 214, 299
XmiI GTMKAC 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.