Rmu_sc0000962.1_g000072

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000962.1
N/A
Physical Location & Seq
Forward (+)
297464 .. 300646
3183 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 957 bp
atgcgtgagaccctttctcgcatacttagcgccgacaacggcgcttcacagccagggaagcccttgaggctttctcaacaccaagcgctcaagcgcttgtttccagtggagtccaccgccggcgaatctgctcatggatttcgatacggctttgggattcgggcactatggtggtggtttcgatgcttctttgcagatctaacattgatagtgctgactgggatattatggttttcctcgaccatggaagtttgcggcgtgatgctggctgcggtagtgcgcggtggtgcagtcgctgcccggacttggctgttacggatggcggtgaggtggagaggtgactcaacaagtcggggttggctctcagttttggtgcacgatggtgacactgacgggccaacgacgcgacgtagtgatgggctttgggtgcccatagaggttgagaggcccacggtaatgggtgtggactcttgggtgcagtcatgggcccattcttttttctcaagctattgggtttgggcttgggtggaatggatgagggccaatgcagcatctggtatggctgtgcacgatgagtgcaaattgaagtttctggagctcaagacaaagaggacctaccgatcaatagtgtacaagattgaggagaagcaaaagcaagttattgtggaagcaactggggatccaacacaaacctatgagaatttcacagatagccttccttctgatgaatgccgttatgctgtttttgatttcgattttcttacaccagagggtgtcccaaaaagcaggatattcttcattgcctggtctcctgatacatcaagggtgagaaacaagatgatctatgcaagctcaaaggacagattcaagagagagcttgatggaattcagatcgagttgcaggcaactgatccatctgagattggccttgatgttattaagagccgtgccagctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

318

Amino Acids

36.27

Weight (kDa)

8.62

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 186 - 317 1e-37 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 427
AccII CGCG 2 cut(s) 282, 406
AciI CCGC 5 cut(s) 117, 255, 272, 282, 323
AclWI GGATC 3 cut(s) 674, 687, 905
AcsI RAATTY 2 cut(s) 700, 885
AfaI GTAC 1 cut(s) 632
AfeI AGCGCT 2 cut(s) 87, 95
AfiI CCNNNNNNNGG 1 cut(s) 306
AgsI TTSAA 2 cut(s) 586, 868
AjnI CCWGG 2 cut(s) 52, 803
AleI CACNNNNGTG 2 cut(s) 169, 381
AluBI AGCT 5 cut(s) 507, 598, 852, 877, 954
AluI AGCT 5 cut(s) 507, 598, 852, 877, 954
Alw21I GWGCWC 3 cut(s) 378, 570, 600
Alw26I GTCTC 2 cut(s) 2, 813
Alw44I GTGCAC 2 cut(s) 374, 566
AlwI GGATC 3 cut(s) 674, 687, 905
AlwNI CAGNNNCTG 2 cut(s) 296, 554
Aor51HI AGCGCT 2 cut(s) 87, 95
AoxI GGCC 5 cut(s) 395, 446, 486, 540, 925
ApaI GGGCCC 1 cut(s) 490
ApaLI GTGCAC 2 cut(s) 374, 566
ApeKI GCWGC 3 cut(s) 269, 296, 548
ApoI RAATTY 2 cut(s) 700, 885
AspLEI GCGC 5 cut(s) 32, 44, 88, 96, 282
AspS9I GGNCC 6 cut(s) 395, 447, 486, 487, 540, 612
AsuC2I CCSGG 1 cut(s) 301
AsuHPI GGTGA 4 cut(s) 337, 350, 395, 838
AvaII GGWCC 1 cut(s) 612
BaeGI GKGCMC 5 cut(s) 166, 378, 432, 490, 570
BamHI GGATCC 1 cut(s) 679
BanI GGYRCC 1 cut(s) 427
BanII GRGCYC 2 cut(s) 490, 600
Bbv12I GWGCWC 3 cut(s) 378, 570, 600
BbvI GCAGC 3 cut(s) 256, 283, 560
BccI CCATC 5 cut(s) 313, 374, 410, 875, 922
BceAI ACGGC 4 cut(s) 55, 163, 717, 930
BciT130I CCWGG 2 cut(s) 54, 805
BcnI CCSGG 1 cut(s) 301
BcoDI GTCTC 2 cut(s) 2, 813
BfoI RGCGCY 4 cut(s) 33, 45, 89, 97
BglI GCCNNNNNGGC 1 cut(s) 67
BglII AGATCT 1 cut(s) 196
BisI GCNGC 4 cut(s) 256, 270, 297, 549
BlsI GCNGC 4 cut(s) 257, 271, 298, 550
Bme1390I CCNGG 3 cut(s) 54, 301, 805
Bme18I GGWCC 1 cut(s) 612
BmgT120I GGNCC 6 cut(s) 395, 447, 486, 487, 540, 612
BmiI GGNNCC 3 cut(s) 429, 488, 681
BmrFI CCNGG 3 cut(s) 54, 301, 805
BmrI ACTGGG 2 cut(s) 228, 684
BmsI GCATC 3 cut(s) 173, 252, 560
BmuI ACTGGG 2 cut(s) 228, 684
BplI GAGNNNNNCTC 2 cut(s) 58, 90
BpmI CTGGAG 1 cut(s) 614
BpuEI CTTGAG 4 cut(s) 74, 85, 487, 584
BpuMI CCSGG 1 cut(s) 301
BsaI GGTCTC 2 cut(s) 2, 813
BsaJI CCNNGG 3 cut(s) 53, 243, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 306
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 3 cut(s) 104, 223, 679
Bse3DI GCAATG 1 cut(s) 798
BseBI CCWGG 2 cut(s) 54, 805
BseDI CCNNGG 3 cut(s) 53, 243, 450
BseGI GGATG 2 cut(s) 324, 540
BseLI CCNNNNNNNGG 1 cut(s) 306
BseMI GCAATG 1 cut(s) 798
BseMII CTCAG 2 cut(s) 378, 909
BseNI ACTGG 3 cut(s) 104, 223, 679
BseRI GAGGAG 1 cut(s) 656
BseSI GKGCMC 5 cut(s) 166, 378, 432, 490, 570
BseXI GCAGC 3 cut(s) 256, 283, 560
BsgI GTGCAG 2 cut(s) 309, 497
Bsh1236I CGCG 2 cut(s) 282, 406
BshFI GGCC 5 cut(s) 397, 448, 488, 542, 927
BshNI GGYRCC 1 cut(s) 427
BsiHKAI GWGCWC 3 cut(s) 378, 570, 600
BsiSI CCGG 2 cut(s) 120, 301
BslFI GGGAC 1 cut(s) 761
BslI CCNNNNNNNGG 1 cut(s) 306
BsmAI GTCTC 2 cut(s) 2, 813
BsmFI GGGAC 1 cut(s) 761
BsmI GAATGC 1 cut(s) 734
BsnI GGCC 5 cut(s) 397, 448, 488, 542, 927
Bso31I GGTCTC 2 cut(s) 2, 813
Bsp120I GGGCCC 1 cut(s) 486
Bsp1286I GDGCHC 6 cut(s) 166, 378, 432, 490, 570, 600
Bsp1407I TGTACA 1 cut(s) 630
Bsp143I GATC 6 cut(s) 196, 620, 679, 840, 891, 910
Bsp19I CCATGG 1 cut(s) 243
BspACI CCGC 5 cut(s) 117, 255, 272, 282, 323
BspANI GGCC 5 cut(s) 397, 448, 488, 542, 927
BspCNI CTCAG 2 cut(s) 377, 910
BspFNI CGCG 2 cut(s) 282, 406
BspLI GGNNCC 3 cut(s) 429, 488, 681
BspPI GGATC 3 cut(s) 674, 687, 905
BspT107I GGYRCC 1 cut(s) 427
BspTNI GGTCTC 2 cut(s) 2, 813
BsrDI GCAATG 1 cut(s) 798
BsrFI RCCGGY 1 cut(s) 119
BsrGI TGTACA 1 cut(s) 630
BsrI ACTGG 3 cut(s) 104, 223, 679
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 53, 243, 450
BssMI GATC 6 cut(s) 196, 620, 679, 840, 891, 910
BssT1I CCWWGG 1 cut(s) 243
Bst2UI CCWGG 2 cut(s) 54, 805
Bst4CI ACNGT 1 cut(s) 454
BstAPI GCANNNNNTGC 1 cut(s) 296
BstAUI TGTACA 1 cut(s) 630
BstC8I GCNNGC 5 cut(s) 121, 267, 850, 903, 952
BstDEI CTNAG 3 cut(s) 26, 364, 918
BstDSI CCRYGG 2 cut(s) 243, 450
BstF5I GGATG 2 cut(s) 324, 540
BstFNI CGCG 2 cut(s) 282, 406
BstH2I RGCGCY 4 cut(s) 33, 45, 89, 97
BstHHI GCGC 5 cut(s) 32, 44, 88, 96, 282
BstKTI GATC 6 cut(s) 199, 623, 682, 843, 894, 913
BstMAI GTCTC 2 cut(s) 2, 813
BstMBI GATC 6 cut(s) 196, 620, 679, 840, 891, 910
BstMWI GCNNNNNNNGC 8 cut(s) 27, 58, 67, 296, 403, 427, 548, 951
BstNI CCWGG 2 cut(s) 54, 805
BstSCI CCNGG 3 cut(s) 52, 299, 803
BstSLI GKGCMC 5 cut(s) 166, 378, 432, 490, 570
BstUI CGCG 2 cut(s) 282, 406
BstV1I GCAGC 3 cut(s) 256, 283, 560
BstX2I RGATCY 2 cut(s) 196, 679
BstXI CCANNNNNNTGG 1 cut(s) 457
BstYI RGATCY 2 cut(s) 196, 679
BsuRI GGCC 5 cut(s) 397, 448, 488, 542, 927
BtgI CCRYGG 2 cut(s) 243, 450
BtsCI GGATG 2 cut(s) 324, 540
BtsIMutI CAGTG 2 cut(s) 111, 387
Cac8I GCNNGC 5 cut(s) 121, 267, 850, 903, 952
CaiI CAGNNNCTG 2 cut(s) 296, 554
CfoI GCGC 5 cut(s) 32, 44, 88, 96, 282
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 6 cut(s) 395, 447, 486, 487, 540, 612
CseI GACGC 1 cut(s) 412
Csp6I GTAC 1 cut(s) 631
CviAII CATG 3 cut(s) 134, 244, 483
CviQI GTAC 1 cut(s) 631
DdeI CTNAG 3 cut(s) 26, 364, 918
DpnI GATC 6 cut(s) 198, 622, 681, 842, 893, 912
DpnII GATC 6 cut(s) 196, 620, 679, 840, 891, 910
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 1 cut(s) 243
Eco24I GRGCYC 2 cut(s) 490, 600
Eco31I GGTCTC 2 cut(s) 2, 813
Eco47I GGWCC 1 cut(s) 612
Eco47III AGCGCT 2 cut(s) 87, 95
Eco53kI GAGCTC 1 cut(s) 598
EcoICRI GAGCTC 1 cut(s) 598
EcoO109I RGGNCCY 1 cut(s) 612
EcoRI GAATTC 1 cut(s) 885
EcoRII CCWGG 2 cut(s) 52, 803
EcoT14I CCWWGG 1 cut(s) 243
EcoT38I GRGCYC 2 cut(s) 490, 600
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 3 cut(s) 137, 247, 486
FaqI GGGAC 1 cut(s) 761
FatI CATG 3 cut(s) 133, 243, 482
Fnu4HI GCNGC 4 cut(s) 256, 270, 297, 549
FokI GGATG 2 cut(s) 331, 547
FriOI GRGCYC 2 cut(s) 490, 600
Fsp4HI GCNGC 4 cut(s) 256, 270, 297, 549
GlaI GCGC 5 cut(s) 31, 43, 87, 95, 281
GluI GCNGC 4 cut(s) 256, 270, 297, 549
GsuI CTGGAG 1 cut(s) 614
HaeII RGCGCY 4 cut(s) 33, 45, 89, 97
HaeIII GGCC 5 cut(s) 397, 448, 488, 542, 927
HapII CCGG 2 cut(s) 120, 301
HgaI GACGC 1 cut(s) 412
HhaI GCGC 5 cut(s) 32, 44, 88, 96, 282
Hin1II CATG 3 cut(s) 137, 247, 486
Hin6I GCGC 5 cut(s) 30, 42, 86, 94, 280
HinP1I GCGC 5 cut(s) 30, 42, 86, 94, 280
HinfI GANTC 6 cut(s) 110, 125, 157, 341, 467, 864
HpaII CCGG 2 cut(s) 120, 301
HphI GGTGA 4 cut(s) 337, 350, 395, 838
Hpy166II GTNNAC 5 cut(s) 114, 376, 466, 568, 631
Hpy188I TCNGA 3 cut(s) 724, 891, 919
Hpy188III TCNNGA 4 cut(s) 593, 601, 812, 868
Hpy8I GTNNAC 5 cut(s) 114, 376, 466, 568, 631
Hpy99I CGWCG 2 cut(s) 406, 411
HpyAV CCTTC 2 cut(s) 725, 729
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4IV ACGT 1 cut(s) 409
HpyCH4V TGCA 9 cut(s) 194, 290, 376, 478, 548, 568, 579, 848, 901
HpyF10VI GCNNNNNNNGC 8 cut(s) 27, 58, 67, 296, 403, 427, 548, 951
HpyF3I CTNAG 3 cut(s) 26, 364, 918
HpySE526I ACGT 1 cut(s) 409
Hsp92II CATG 3 cut(s) 137, 247, 486
HspAI GCGC 5 cut(s) 30, 42, 86, 94, 280
KroI GCCGGC 1 cut(s) 119
KroNI GCCGGC 1 cut(s) 121
Kzo9I GATC 6 cut(s) 196, 620, 679, 840, 891, 910
LmnI GCTCC 1 cut(s) 595
Lsp1109I GCAGC 3 cut(s) 256, 283, 560
LweI GCATC 3 cut(s) 173, 252, 560
MaeII ACGT 1 cut(s) 409
MaeIII GTNAC 3 cut(s) 312, 338, 383
MalI GATC 6 cut(s) 198, 622, 681, 842, 893, 912
MboI GATC 6 cut(s) 196, 620, 679, 840, 891, 910
MboII GAAGA 1 cut(s) 787
MflI RGATCY 2 cut(s) 196, 679
MhlI GDGCHC 6 cut(s) 166, 378, 432, 490, 570, 600
MluCI AATT 3 cut(s) 581, 700, 885
MlyI GAGTC 3 cut(s) 119, 335, 461
MmeI TCCRAC 1 cut(s) 707
MreI CGCCGGCG 1 cut(s) 119
MroNI GCCGGC 1 cut(s) 119
MseI TTAA 1 cut(s) 939
MslI CAYNNNNRTG 3 cut(s) 169, 381, 455
MspI CCGG 2 cut(s) 120, 301
MspR9I CCNGG 3 cut(s) 54, 301, 805
Mva1269I GAATGC 1 cut(s) 734
MvaI CCWGG 2 cut(s) 54, 805
MvnI CGCG 2 cut(s) 282, 406
MwoI GCNNNNNNNGC 8 cut(s) 27, 58, 67, 296, 403, 427, 548, 951
NaeI GCCGGC 1 cut(s) 121
NciI CCSGG 1 cut(s) 301
NcoI CCATGG 1 cut(s) 243
NdeII GATC 6 cut(s) 196, 620, 679, 840, 891, 910
NgoMIV GCCGGC 1 cut(s) 119
NlaIII CATG 3 cut(s) 137, 247, 486
NlaIV GGNNCC 3 cut(s) 429, 488, 681
NmuCI GTSAC 2 cut(s) 338, 383
OliI CACNNNNGTG 2 cut(s) 169, 381
PctI GAATGC 1 cut(s) 734
PdiI GCCGGC 1 cut(s) 121
PfeI GAWTC 3 cut(s) 125, 157, 864
PkrI GCNGC 4 cut(s) 257, 271, 298, 550
PleI GAGTC 3 cut(s) 118, 335, 461
PpsI GAGTC 3 cut(s) 118, 335, 461
PpuMI RGGWCCY 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 600
Psp5II RGGWCCY 1 cut(s) 612
Psp6I CCWGG 2 cut(s) 52, 803
PspGI CCWGG 2 cut(s) 52, 803
PspN4I GGNNCC 3 cut(s) 429, 488, 681
PspOMI GGGCCC 1 cut(s) 486
PspPI GGNCC 6 cut(s) 395, 447, 486, 487, 540, 612
PspPPI RGGWCCY 1 cut(s) 612
PstNI CAGNNNCTG 2 cut(s) 296, 554
PsuI RGATCY 2 cut(s) 196, 679
RsaI GTAC 1 cut(s) 632
RsaNI GTAC 1 cut(s) 631
RseI CAYNNNNRTG 3 cut(s) 169, 381, 455
SacI GAGCTC 1 cut(s) 600
SaqAI TTAA 1 cut(s) 939
SatI GCNGC 4 cut(s) 256, 270, 297, 549
Sau3AI GATC 6 cut(s) 196, 620, 679, 840, 891, 910
Sau96I GGNCC 6 cut(s) 395, 447, 486, 487, 540, 612
SchI GAGTC 3 cut(s) 119, 335, 461
ScrFI CCNGG 3 cut(s) 54, 301, 805
SduI GDGCHC 6 cut(s) 166, 378, 432, 490, 570, 600
SfaNI GCATC 3 cut(s) 173, 252, 560
SgrAI CRCCGGYG 1 cut(s) 119
SinI GGWCC 1 cut(s) 612
SmiMI CAYNNNNRTG 3 cut(s) 169, 381, 455
SmlI CTYRAG 4 cut(s) 64, 89, 502, 599
SmoI CTYRAG 4 cut(s) 64, 89, 502, 599
Sse9I AATT 3 cut(s) 581, 700, 885
SsiI CCGC 5 cut(s) 117, 255, 272, 282, 323
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 3 cut(s) 52, 299, 803
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 1 cut(s) 454
TaiI ACGT 1 cut(s) 412
TaqI TCGA 5 cut(s) 142, 181, 239, 753, 894
TasI AATT 3 cut(s) 581, 700, 885
TatI WGTACW 1 cut(s) 630
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 3 cut(s) 125, 157, 864
Tru1I TTAA 1 cut(s) 939
Tru9I TTAA 1 cut(s) 939
TscAI CASTG 2 cut(s) 111, 394
TseFI GTSAC 2 cut(s) 338, 383
TseI GCWGC 3 cut(s) 269, 296, 548
Tsp45I GTSAC 2 cut(s) 338, 383
TspDTI ATGAA 2 cut(s) 741, 787
TspGWI ACGGA 1 cut(s) 331
TspRI CASTG 2 cut(s) 111, 394
VneI GTGCAC 2 cut(s) 374, 566
VpaK11BI GGWCC 1 cut(s) 612
XapI RAATTY 2 cut(s) 700, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.