Rmu_sc0001043.1_g000007

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001043.1
N/A
Physical Location & Seq
Forward (+)
21331 .. 22113
783 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 648 bp
atgggatccgaatatgcacaggtgcaccatctatttcccatcattttttcgctgatgattcttttctttttttccaaggctgaacaagaggagtgtgttgtgttgaaagatattttcaagcaatatgaagttgcctcaggtcagctcatcaattatcagaagagctctgtttcctttagtaagaatgtgaggatgagcaagaaacacgagcttgcgtctatcatggttgtcatgcgagttgacaaacatgaaaaataccttggcctccctacggagataaactactccatggctgaagctttcaattttctaacagagagggagatgcataggttaatggataatttttggtggggtgataaatcgaatgcaaagaagatccattggtgtgcttgggagaaactctgtgttccaaagaatgaaggtggcctagggtttcggaatatgaagctctttaaccaagggcttctggcgaagcaaggttggagaatcttacaaaatcctacgtcccttcactacaccaaaactgggcttttaaggcttgccatgtgcgtcgtaaaagcctatttacggcgcacaaaaacaggctataaatgctcgcgtcgtaaaagacatatctcattcacggcgcgcttttgtgagccataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

215

Amino Acids

25.28

Weight (kDa)

9.72

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 601, 631
AclWI GGATC 2 cut(s) 13, 373
AcuI CTGAAG 1 cut(s) 315
AfiI CCNNNNNNNGG 3 cut(s) 271, 528, 570
AgsI TTSAA 3 cut(s) 106, 118, 304
AjuI GAANNNNNNNTTGG 2 cut(s) 243, 275
AluBI AGCT 5 cut(s) 145, 165, 211, 299, 451
AluI AGCT 5 cut(s) 145, 165, 211, 299, 451
Alw21I GWGCWC 2 cut(s) 27, 167
Alw44I GTGCAC 1 cut(s) 23
AlwI GGATC 2 cut(s) 13, 373
AoxI GGCC 2 cut(s) 262, 427
ApaLI GTGCAC 1 cut(s) 23
AspA2I CCTAGG 1 cut(s) 430
AspLEI GCGC 3 cut(s) 576, 631, 633
AsuHPI GGTGA 1 cut(s) 368
AvrII CCTAGG 1 cut(s) 430
AxyI CCTNAGG 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 27
BamHI GGATCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 167
BauI CACGAG 1 cut(s) 206
Bbv12I GWGCWC 2 cut(s) 27, 167
BccI CCATC 2 cut(s) 36, 47
BceAI ACGGC 2 cut(s) 587, 642
BfaI CTAG 1 cut(s) 431
BlnI CCTAGG 1 cut(s) 430
BmiI GGNNCC 1 cut(s) 7
BmrI ACTGGG 1 cut(s) 537
BmsI GCATC 1 cut(s) 315
BmuI ACTGGG 1 cut(s) 537
BsaJI CCNNGG 5 cut(s) 75, 259, 288, 430, 460
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 528, 570
Bse1I ACTGG 1 cut(s) 532
Bse21I CCTNAGG 1 cut(s) 136
BseDI CCNNGG 5 cut(s) 75, 259, 288, 430, 460
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 3 cut(s) 271, 528, 570
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 1 cut(s) 532
BsePI GCGCGC 1 cut(s) 629
BseRI GAGGAG 1 cut(s) 104
BseSI GKGCMC 1 cut(s) 27
Bsh1236I CGCG 2 cut(s) 601, 631
BshFI GGCC 2 cut(s) 264, 429
BsiHKAI GWGCWC 2 cut(s) 27, 167
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 3 cut(s) 271, 528, 570
BsmFI GGGAC 1 cut(s) 493
BsmI GAATGC 1 cut(s) 373
BsnI GGCC 2 cut(s) 264, 429
Bsp1286I GDGCHC 2 cut(s) 27, 167
Bsp143I GATC 2 cut(s) 5, 378
Bsp19I CCATGG 1 cut(s) 288
BspANI GGCC 2 cut(s) 264, 429
BspCNI CTCAG 1 cut(s) 149
BspFNI CGCG 2 cut(s) 601, 631
BspLI GGNNCC 1 cut(s) 7
BspPI GGATC 2 cut(s) 13, 373
BspQI GCTCTTC 1 cut(s) 155
BsrI ACTGG 1 cut(s) 532
BssECI CCNNGG 5 cut(s) 75, 259, 288, 430, 460
BssHII GCGCGC 1 cut(s) 629
BssMI GATC 2 cut(s) 5, 378
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 5 cut(s) 75, 259, 288, 430, 460
Bst2BI CACGAG 1 cut(s) 206
Bst6I CTCTTC 1 cut(s) 155
BstC8I GCNNGC 4 cut(s) 213, 543, 599, 631
BstDEI CTNAG 1 cut(s) 136
BstDSI CCRYGG 1 cut(s) 288
BstF5I GGATG 1 cut(s) 198
BstFNI CGCG 2 cut(s) 601, 631
BstHHI GCGC 3 cut(s) 576, 631, 633
BstKTI GATC 2 cut(s) 8, 381
BstMBI GATC 2 cut(s) 5, 378
BstMWI GCNNNNNNNGC 2 cut(s) 538, 594
BstSLI GKGCMC 1 cut(s) 27
BstUI CGCG 2 cut(s) 601, 631
BstX2I RGATCY 2 cut(s) 5, 378
BstYI RGATCY 2 cut(s) 5, 378
Bsu36I CCTNAGG 1 cut(s) 136
BsuRI GGCC 2 cut(s) 264, 429
BtgI CCRYGG 1 cut(s) 288
BtsCI GGATG 1 cut(s) 198
Cac8I GCNNGC 4 cut(s) 213, 543, 599, 631
CfoI GCGC 3 cut(s) 576, 631, 633
CseI GACGC 3 cut(s) 204, 541, 590
CviAII CATG 5 cut(s) 223, 232, 248, 289, 547
DdeI CTNAG 1 cut(s) 136
DpnI GATC 2 cut(s) 7, 380
DpnII GATC 2 cut(s) 5, 378
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
Ecl136II GAGCTC 1 cut(s) 165
Eco130I CCWWGG 5 cut(s) 75, 259, 288, 430, 460
Eco24I GRGCYC 1 cut(s) 167
Eco53kI GAGCTC 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 315
Eco81I CCTNAGG 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 165
EcoT14I CCWWGG 5 cut(s) 75, 259, 288, 430, 460
EcoT22I ATGCAT 1 cut(s) 330
EcoT38I GRGCYC 1 cut(s) 167
ErhI CCWWGG 5 cut(s) 75, 259, 288, 430, 460
FaeI CATG 5 cut(s) 226, 235, 251, 292, 550
FaqI GGGAC 1 cut(s) 493
FatI CATG 5 cut(s) 222, 231, 247, 288, 546
FokI GGATG 1 cut(s) 205
FriOI GRGCYC 1 cut(s) 167
FspBI CTAG 1 cut(s) 431
GlaI GCGC 3 cut(s) 575, 630, 632
HaeIII GGCC 2 cut(s) 264, 429
HgaI GACGC 3 cut(s) 204, 541, 590
HhaI GCGC 3 cut(s) 576, 631, 633
Hin1II CATG 5 cut(s) 226, 235, 251, 292, 550
Hin6I GCGC 3 cut(s) 574, 629, 631
HinP1I GCGC 3 cut(s) 574, 629, 631
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HindIII AAGCTT 1 cut(s) 297
HinfI GANTC 2 cut(s) 58, 489
HphI GGTGA 1 cut(s) 368
Hpy166II GTNNAC 2 cut(s) 25, 241
Hpy188I TCNGA 3 cut(s) 10, 159, 441
Hpy8I GTNNAC 2 cut(s) 25, 241
Hpy99I CGWCG 2 cut(s) 557, 606
HpyAV CCTTC 2 cut(s) 416, 521
HpyCH4IV ACGT 1 cut(s) 506
HpyCH4V TGCA 4 cut(s) 17, 25, 328, 371
HpyF10VI GCNNNNNNNGC 2 cut(s) 538, 594
HpyF3I CTNAG 1 cut(s) 136
HpySE526I ACGT 1 cut(s) 506
Hsp92II CATG 5 cut(s) 226, 235, 251, 292, 550
HspAI GCGC 3 cut(s) 574, 629, 631
Kzo9I GATC 2 cut(s) 5, 378
LguI GCTCTTC 1 cut(s) 155
LpnPI CCDG 5 cut(s) 5, 123, 455, 513, 570
LweI GCATC 1 cut(s) 315
MaeI CTAG 1 cut(s) 431
MaeII ACGT 1 cut(s) 506
MalI GATC 2 cut(s) 7, 380
MboI GATC 2 cut(s) 5, 378
MboII GAAGA 2 cut(s) 172, 388
MflI RGATCY 2 cut(s) 5, 378
MhlI GDGCHC 2 cut(s) 27, 167
MluCI AATT 3 cut(s) 151, 304, 343
MmeI TCCRAC 1 cut(s) 464
MnlI CCTC 5 cut(s) 82, 145, 183, 275, 312
Mph1103I ATGCAT 1 cut(s) 330
MseI TTAA 3 cut(s) 335, 456, 536
MslI CAYNNNNRTG 1 cut(s) 387
Mva1269I GAATGC 1 cut(s) 373
MvnI CGCG 2 cut(s) 601, 631
MwoI GCNNNNNNNGC 2 cut(s) 538, 594
NcoI CCATGG 1 cut(s) 288
NdeII GATC 2 cut(s) 5, 378
NlaIII CATG 5 cut(s) 226, 235, 251, 292, 550
NlaIV GGNNCC 1 cut(s) 7
NsiI ATGCAT 1 cut(s) 330
PauI GCGCGC 1 cut(s) 629
PciSI GCTCTTC 1 cut(s) 155
PctI GAATGC 1 cut(s) 373
PfeI GAWTC 2 cut(s) 58, 489
Psp124BI GAGCTC 1 cut(s) 167
PspN4I GGNNCC 1 cut(s) 7
PsuI RGATCY 2 cut(s) 5, 378
PteI GCGCGC 1 cut(s) 629
RseI CAYNNNNRTG 1 cut(s) 387
SacI GAGCTC 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 155
SaqAI TTAA 3 cut(s) 335, 456, 536
Sau3AI GATC 2 cut(s) 5, 378
SduI GDGCHC 2 cut(s) 27, 167
SfaNI GCATC 1 cut(s) 315
SmiMI CAYNNNNRTG 1 cut(s) 387
Sse9I AATT 3 cut(s) 151, 304, 343
SspMI CTAG 1 cut(s) 431
SstI GAGCTC 1 cut(s) 167
StyI CCWWGG 5 cut(s) 75, 259, 288, 430, 460
TaiI ACGT 1 cut(s) 509
TaqI TCGA 1 cut(s) 365
TasI AATT 3 cut(s) 151, 304, 343
TfiI GAWTC 2 cut(s) 58, 489
Tru1I TTAA 3 cut(s) 335, 456, 536
Tru9I TTAA 3 cut(s) 335, 456, 536
TspDTI ATGAA 4 cut(s) 141, 264, 435, 461
TspGWI ACGGA 1 cut(s) 287
VneI GTGCAC 1 cut(s) 23
XmaJI CCTAGG 1 cut(s) 430
XspI CTAG 1 cut(s) 431
Zsp2I ATGCAT 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.