Rmu_sc0001070.1_g000003

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001070.1
N/A
Physical Location & Seq
Reverse (-)
9063 .. 11057
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 594 bp
atggccaacgttggcttgtttgcgcagcagggatgcttcggtggcagcaggctggaacgtagggctgcaggaagggggaagcaggggctgctgtgcaaggtgcatgaagcgattgcactagaagagtctcaagaattcccggtgggaattgtcattcaagaaattcgccatggctttttacttggtggtcgacttctaagaccagcaatggttattgtctctatgggcctggcagggtgggttcggttcggtaccggacattcaagaccaataccatctaccgtaccagacactcttggtacacaaaataggctgccattaccggccacaaaagtcggtataccaacttctcggtatcggttggtatcggttggagctcctaaacctcttgaattgttgagattggagcaacacctgaaaattagtaattacaataagagattttctgggtttgaagctttgaatcaaatcgaacttcaagccgtcgatgcctcaaatcgaatcgaatcgaagagttctgggtttgaagctttgaatcgaattgaacttcaagccttcgaggcctcgaatgaaatcgaagagttctgggtttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

197

Amino Acids

21.68

Weight (kDa)

9.34

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 33 - 74 4.8e-10 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 24
Acc65I GGTACC 1 cut(s) 251
AccB1I GGYRCC 1 cut(s) 251
AccI GTMKAC 2 cut(s) 190, 340
AclI AACGTT 1 cut(s) 9
AcoI YGGCCR 2 cut(s) 3, 324
AcsI RAATTY 2 cut(s) 134, 162
AfaI GTAC 3 cut(s) 253, 285, 301
AjnI CCWGG 1 cut(s) 228
AluBI AGCT 3 cut(s) 377, 458, 530
AluI AGCT 3 cut(s) 377, 458, 530
Alw21I GWGCWC 1 cut(s) 379
Alw26I GTCTC 2 cut(s) 132, 223
AlwNI CAGNNNCTG 1 cut(s) 88
AoxI GGCC 4 cut(s) 3, 226, 324, 561
ApeKI GCWGC 5 cut(s) 25, 45, 65, 88, 313
ApoI RAATTY 2 cut(s) 134, 162
Asp718I GGTACC 1 cut(s) 251
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 1 cut(s) 226
AsuC2I CCSGG 1 cut(s) 140
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 1 cut(s) 379
BarI GAAGNNNNNNTAC 2 cut(s) 331, 363
Bbv12I GWGCWC 1 cut(s) 379
BbvI GCAGC 5 cut(s) 37, 52, 57, 75, 300
BccI CCATC 1 cut(s) 283
BceAI ACGGC 1 cut(s) 467
BciT130I CCWGG 1 cut(s) 230
BcnI CCSGG 1 cut(s) 140
BcoDI GTCTC 2 cut(s) 132, 223
BfaI CTAG 1 cut(s) 119
BfmI CTRYAG 1 cut(s) 66
BglI GCCNNNNNGGC 1 cut(s) 560
BisI GCNGC 5 cut(s) 26, 46, 66, 89, 314
BlsI GCNGC 5 cut(s) 27, 47, 67, 90, 315
Bme1390I CCNGG 2 cut(s) 140, 230
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 2 cut(s) 140, 230
BmsI GCATC 2 cut(s) 23, 478
BpuEI CTTGAG 1 cut(s) 114
BpuMI CCSGG 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 169
BsaWI WCCGGW 1 cut(s) 254
Bse118I RCCGGY 1 cut(s) 322
Bse3DI GCAATG 1 cut(s) 213
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 1 cut(s) 169
BseGI GGATG 1 cut(s) 38
BseMI GCAATG 1 cut(s) 213
BseXI GCAGC 5 cut(s) 37, 52, 57, 75, 300
BshFI GGCC 4 cut(s) 5, 228, 326, 563
BshNI GGYRCC 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 379
BsiSI CCGG 3 cut(s) 140, 255, 323
BsmAI GTCTC 2 cut(s) 132, 223
BsnI GGCC 4 cut(s) 5, 228, 326, 563
Bsp1286I GDGCHC 1 cut(s) 379
Bsp19I CCATGG 1 cut(s) 169
BspANI GGCC 4 cut(s) 5, 228, 326, 563
BspLI GGNNCC 1 cut(s) 253
BspMAI CTGCAG 1 cut(s) 70
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 213
BsrFI RCCGGY 1 cut(s) 322
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 1 cut(s) 169
BssNAI GTATAC 1 cut(s) 341
BssT1I CCWWGG 1 cut(s) 169
Bst1107I GTATAC 1 cut(s) 341
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 283
Bst6I CTCTTC 3 cut(s) 117, 506, 573
BstAPI GCANNNNNTGC 1 cut(s) 88
BstC8I GCNNGC 1 cut(s) 50
BstDEI CTNAG 1 cut(s) 197
BstDSI CCRYGG 1 cut(s) 169
BstF5I GGATG 1 cut(s) 38
BstHHI GCGC 1 cut(s) 25
BstMAI GTCTC 2 cut(s) 132, 223
BstMWI GCNNNNNNNGC 4 cut(s) 42, 88, 488, 560
BstNI CCWGG 1 cut(s) 230
BstSCI CCNGG 2 cut(s) 138, 228
BstSFI CTRYAG 1 cut(s) 66
BstV1I GCAGC 5 cut(s) 37, 52, 57, 75, 300
BstZ17I GTATAC 1 cut(s) 341
BsuRI GGCC 4 cut(s) 5, 228, 326, 563
BtgI CCRYGG 1 cut(s) 169
BtsCI GGATG 1 cut(s) 38
Cac8I GCNNGC 1 cut(s) 50
CaiI CAGNNNCTG 1 cut(s) 88
CfoI GCGC 1 cut(s) 25
Cfr10I RCCGGY 1 cut(s) 322
Cfr13I GGNCC 1 cut(s) 226
Csp6I GTAC 3 cut(s) 252, 284, 300
CviAII CATG 2 cut(s) 104, 170
CviQI GTAC 3 cut(s) 252, 284, 300
DdeI CTNAG 1 cut(s) 197
EaeI YGGCCR 2 cut(s) 3, 324
Eam1104I CTCTTC 3 cut(s) 117, 506, 573
EarI CTCTTC 3 cut(s) 117, 506, 573
Ecl136II GAGCTC 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 169
Eco147I AGGCCT 1 cut(s) 563
Eco24I GRGCYC 1 cut(s) 379
Eco53kI GAGCTC 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 377
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 2 cut(s) 107, 173
FaiI YATR 4 cut(s) 105, 171, 224, 341
FatI CATG 2 cut(s) 103, 169
FblI GTMKAC 2 cut(s) 190, 340
Fnu4HI GCNGC 5 cut(s) 26, 46, 66, 89, 314
FokI GGATG 1 cut(s) 45
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 5 cut(s) 26, 46, 66, 89, 314
FspBI CTAG 1 cut(s) 119
FspI TGCGCA 1 cut(s) 24
GlaI GCGC 1 cut(s) 24
GluI GCNGC 5 cut(s) 26, 46, 66, 89, 314
HaeIII GGCC 4 cut(s) 5, 228, 326, 563
HapII CCGG 3 cut(s) 140, 255, 323
HhaI GCGC 1 cut(s) 25
Hin1II CATG 2 cut(s) 107, 173
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HincII GTYRAC 1 cut(s) 191
HindII GTYRAC 1 cut(s) 191
HindIII AAGCTT 2 cut(s) 456, 528
HinfI GANTC 5 cut(s) 125, 463, 501, 506, 535
HpaII CCGG 3 cut(s) 140, 255, 323
Hpy166II GTNNAC 3 cut(s) 191, 302, 341
Hpy188III TCNNGA 4 cut(s) 131, 158, 264, 389
Hpy8I GTNNAC 3 cut(s) 191, 302, 341
Hpy99I CGWCG 1 cut(s) 488
HpyAV CCTTC 2 cut(s) 66, 565
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 2 cut(s) 9, 58
HpyCH4V TGCA 4 cut(s) 68, 96, 103, 116
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 88, 488, 560
HpyF3I CTNAG 1 cut(s) 197
HpySE526I ACGT 2 cut(s) 9, 58
Hsp92II CATG 2 cut(s) 107, 173
HspAI GCGC 1 cut(s) 23
KpnI GGTACC 1 cut(s) 255
LmnI GCTCC 3 cut(s) 374, 382, 406
Lsp1109I GCAGC 5 cut(s) 37, 52, 57, 75, 300
LweI GCATC 2 cut(s) 23, 478
MaeI CTAG 1 cut(s) 119
MaeII ACGT 2 cut(s) 9, 58
MboII GAAGA 3 cut(s) 134, 523, 590
MhlI GDGCHC 1 cut(s) 379
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 134, 147, 162, 392, 420, 427, 540
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 352
MnlI CCTC 4 cut(s) 396, 502, 553, 574
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 140, 255, 323
MspR9I CCNGG 2 cut(s) 140, 230
MvaI CCWGG 1 cut(s) 230
MwoI GCNNNNNNNGC 4 cut(s) 42, 88, 488, 560
NciI CCSGG 1 cut(s) 140
NcoI CCATGG 1 cut(s) 169
NlaIII CATG 2 cut(s) 107, 173
NlaIV GGNNCC 1 cut(s) 253
NsbI TGCGCA 1 cut(s) 24
PceI AGGCCT 1 cut(s) 563
PfeI GAWTC 4 cut(s) 463, 501, 506, 535
PkrI GCNGC 5 cut(s) 27, 47, 67, 90, 315
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
Psp124BI GAGCTC 1 cut(s) 379
Psp1406I AACGTT 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 1 cut(s) 253
PspPI GGNCC 1 cut(s) 226
PstI CTGCAG 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 88
RsaI GTAC 3 cut(s) 253, 285, 301
RsaNI GTAC 3 cut(s) 252, 284, 300
SacI GAGCTC 1 cut(s) 379
SalI GTCGAC 1 cut(s) 189
SatI GCNGC 5 cut(s) 26, 46, 66, 89, 314
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 2 cut(s) 140, 230
SduI GDGCHC 1 cut(s) 379
SetI ASST 8 cut(s) 12, 61, 102, 379, 388, 417, 460, 532
SfaNI GCATC 2 cut(s) 23, 478
SfcI CTRYAG 1 cut(s) 66
SmlI CTYRAG 1 cut(s) 129
SmoI CTYRAG 1 cut(s) 129
Sse9I AATT 7 cut(s) 134, 147, 162, 392, 420, 427, 540
SseBI AGGCCT 1 cut(s) 563
SspMI CTAG 1 cut(s) 119
SstI GAGCTC 1 cut(s) 379
StuI AGGCCT 1 cut(s) 563
StyD4I CCNGG 2 cut(s) 138, 228
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 2 cut(s) 12, 61
TasI AATT 7 cut(s) 134, 147, 162, 392, 420, 427, 540
TfiI GAWTC 4 cut(s) 463, 501, 506, 535
TseI GCWGC 5 cut(s) 25, 45, 65, 88, 313
TspDTI ATGAA 2 cut(s) 120, 585
XapI RAATTY 2 cut(s) 134, 162
XmiI GTMKAC 2 cut(s) 190, 340
XspI CTAG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.