Rmu_sc0001158.1_g000002

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001158.1
N/A
Physical Location & Seq
Reverse (-)
13001 .. 17596
4596 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1419 bp
atggtggttctcgtctggcatgccctagcgtcagcgtgggcctttggctcggctagtgtgtggggattgctgccggacgggttttgggaggctctatggtggtcgaggtggagtgctagagcagcatggctatttcttctagatggcggctacgacaattctacagtttcatgcttgacaatgggcaaggaagcatgctgggtgatgggtcactatggcagtggagtatgcaaggtttgcttggtgtcctattggtggtctagatcttttgggggggaagacggtgccattttctgttcggcgatgatggttattaactatcccatggtagttgtttgggacaagtatgtagaatctggcaacacccttttggatagcgatgtcagtctccaccgtcacaagcattcagctcagtcactcattaccagagagaaccagaggacgaggatgagctgcggagaaagcaaggttgtgtgtgtgacaggagcatctgggttcatagcatcatggctagtcaagctcttattgcaacgaggttatactgtcaaagccactgttcgggacccaaatgatcgaaagaaaacagagcacctactctcatttgatggagcaaaggaaaggcttcatttgttcaaagcagacttgttagaagaaggttcttttgaccctgtcgttgatgggtgtgaaggtgtttttcatacagcatcccctgtcctactctcatctactaatccgcaggcagaattaattgagcctgctttgaagggaacgcttaatgtccttggatcgtgtgtgaaggtccagtctatcaaaagggtggttataacatcctctatggcagcagttagatttaatggaaaacctcttactgctgatgtaataatcgatgaatcttggttttcagatcctgctttttgtgaaaaagaaaagctttggtatatgctttcaaagacgttagctgaggaagctgcttggaagtttgcaaaagagaaaggaattgatattattacaataaatccgggatgggtgatcggccctcccttacagccaactctgaacttaactgtggaactagttctgaaactcgtaaatgggaccgaaaagtttcccaacgacacctacagatttgttgatgttagagatgttgctaatgcacatattctagccttggaaaacccatctgctagtggacgttattgtttagttggaagcgtaaaacactgttcagaggttgtgaaaatgttgcacgagatcttccctgctctcaatcttccagataaatgtgaagatgtcaagcctttcacaccaacttaccaggtatccaaggaaagaacccaaactttgggtgtaaaatatactccgcttgaagtgtctctgaaggatactgttgaaagtttgaaggacaagaacttcttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

472

Amino Acids

52.53

Weight (kDa)

6.43

Isoelectric Point (pI)

31.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 155 - 241 7.7e-08 NmrA-like family
Epimerase PF01370 158 - 396 9.3e-24 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 160 - 392 3.4e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 160 - 349 8.3e-12 Male sterility protein
GDP_Man_Dehyd PF16363 160 - 406 3.1e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 162 - 284 5.4e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 824
AccB1I GGYRCC 1 cut(s) 284
AccB7I CCANNNNNTGG 1 cut(s) 1342
AciI CCGC 4 cut(s) 147, 456, 734, 1361
AclWI GGATC 2 cut(s) 793, 899
AcuI CTGAAG 1 cut(s) 1397
AfiI CCNNNNNNNGG 3 cut(s) 255, 558, 1342
AgsI TTSAA 6 cut(s) 634, 763, 948, 1367, 1391, 1399
AhlI ACTAGT 1 cut(s) 1072
AjnI CCWGG 1 cut(s) 1314
AluBI AGCT 6 cut(s) 410, 453, 520, 931, 959, 968
AluI AGCT 6 cut(s) 410, 453, 520, 931, 959, 968
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 2 cut(s) 392, 1377
AlwI GGATC 2 cut(s) 793, 899
AlwNI CAGNNNCTG 1 cut(s) 908
AoxI GGCC 2 cut(s) 39, 1033
ApeKI GCWGC 5 cut(s) 70, 122, 453, 839, 968
AseI ATTAAT 1 cut(s) 746
Asp700I GAANNNNTTC 4 cut(s) 621, 1074, 1104, 1412
AspS9I GGNCC 5 cut(s) 39, 562, 799, 1034, 1095
AsuC2I CCSGG 1 cut(s) 1020
AsuHPI GGTGA 2 cut(s) 214, 1039
AvaII GGWCC 3 cut(s) 562, 799, 1095
BanI GGYRCC 1 cut(s) 284
BauI CACGAG 1 cut(s) 1247
BbsI GAAGAC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 591
BbvCI CCTCAGC 1 cut(s) 960
BbvI GCAGC 5 cut(s) 57, 134, 440, 851, 955
BccI CCATC 7 cut(s) 137, 199, 301, 599, 671, 1017, 1186
BciT130I CCWGG 1 cut(s) 1316
BciVI GTATCC 2 cut(s) 1330, 1375
BcnI CCSGG 1 cut(s) 1020
BcoDI GTCTC 2 cut(s) 392, 1377
BcuI ACTAGT 1 cut(s) 1072
BfaI CTAG 9 cut(s) 26, 54, 117, 140, 261, 512, 1073, 1163, 1185
BfmI CTRYAG 2 cut(s) 162, 1120
BfuI GTATCC 2 cut(s) 1330, 1375
BglII AGATCT 2 cut(s) 263, 1251
BisI GCNGC 6 cut(s) 71, 123, 148, 454, 840, 969
BlsI GCNGC 6 cut(s) 72, 124, 149, 455, 841, 970
Bme1390I CCNGG 2 cut(s) 1020, 1316
Bme18I GGWCC 3 cut(s) 562, 799, 1095
BmgT120I GGNCC 5 cut(s) 39, 562, 799, 1034, 1095
BmiI GGNNCC 4 cut(s) 286, 563, 564, 1096
BmrFI CCNGG 2 cut(s) 1020, 1316
BmsI GCATC 3 cut(s) 497, 512, 713
BpiI GAAGAC 1 cut(s) 285
Bpu10I CCTNAGC 1 cut(s) 960
BpuMI CCSGG 1 cut(s) 1020
Bsa29I ATCGAT 1 cut(s) 885
BsaJI CCNNGG 4 cut(s) 324, 781, 1167, 1323
Bsc4I CCNNNNNNNGG 3 cut(s) 255, 558, 1342
Bse1I ACTGG 1 cut(s) 802
BseBI CCWGG 1 cut(s) 1316
BseCI ATCGAT 1 cut(s) 885
BseDI CCNNGG 4 cut(s) 324, 781, 1167, 1323
BseGI GGATG 4 cut(s) 453, 704, 827, 1028
BseLI CCNNNNNNNGG 3 cut(s) 255, 558, 1342
BseMII CTCAG 2 cut(s) 425, 951
BseNI ACTGG 1 cut(s) 802
BseXI GCAGC 5 cut(s) 57, 134, 440, 851, 955
BseYI CCCAGC 1 cut(s) 198
BshFI GGCC 2 cut(s) 41, 1035
BshNI GGYRCC 1 cut(s) 284
BshVI ATCGAT 1 cut(s) 885
BsiHKAI GWGCWC 1 cut(s) 591
BsiSI CCGG 2 cut(s) 74, 1019
BslFI GGGAC 3 cut(s) 353, 575, 1108
BslI CCNNNNNNNGG 3 cut(s) 255, 558, 1342
BsmAI GTCTC 2 cut(s) 392, 1377
BsmFI GGGAC 3 cut(s) 353, 575, 1108
BsmI GAATGC 1 cut(s) 403
BsnI GGCC 2 cut(s) 41, 1035
Bsp1286I GDGCHC 1 cut(s) 591
Bsp143I GATC 6 cut(s) 263, 571, 785, 904, 1029, 1251
Bsp19I CCATGG 1 cut(s) 324
BspACI CCGC 4 cut(s) 147, 456, 734, 1361
BspANI GGCC 2 cut(s) 41, 1035
BspCNI CTCAG 2 cut(s) 424, 952
BspDI ATCGAT 1 cut(s) 885
BspLI GGNNCC 4 cut(s) 286, 563, 564, 1096
BspPI GGATC 2 cut(s) 793, 899
BspT107I GGYRCC 1 cut(s) 284
BsrI ACTGG 1 cut(s) 802
BssECI CCNNGG 4 cut(s) 324, 781, 1167, 1323
BssMI GATC 6 cut(s) 263, 571, 785, 904, 1029, 1251
BssSI CACGAG 1 cut(s) 1247
BssT1I CCWWGG 4 cut(s) 324, 781, 1167, 1323
Bst2BI CACGAG 1 cut(s) 1247
Bst2UI CCWGG 1 cut(s) 1316
Bst4CI ACNGT 8 cut(s) 166, 284, 395, 544, 556, 1066, 1223, 1387
BstAPI GCANNNNNTGC 1 cut(s) 237
BstC8I GCNNGC 4 cut(s) 21, 196, 738, 756
BstDEI CTNAG 2 cut(s) 411, 960
BstDSI CCRYGG 1 cut(s) 324
BstF5I GGATG 4 cut(s) 453, 704, 827, 1028
BstKTI GATC 6 cut(s) 266, 574, 788, 907, 1032, 1254
BstMAI GTCTC 2 cut(s) 392, 1377
BstMBI GATC 6 cut(s) 263, 571, 785, 904, 1029, 1251
BstMWI GCNNNNNNNGC 6 cut(s) 122, 237, 462, 517, 526, 965
BstNI CCWGG 1 cut(s) 1316
BstNSI RCATGY 2 cut(s) 23, 198
BstSCI CCNGG 2 cut(s) 1018, 1314
BstSFI CTRYAG 2 cut(s) 162, 1120
BstV1I GCAGC 5 cut(s) 57, 134, 440, 851, 955
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 3 cut(s) 263, 904, 1251
BstYI RGATCY 3 cut(s) 263, 904, 1251
Bsu15I ATCGAT 1 cut(s) 885
BsuI GTATCC 2 cut(s) 1330, 1375
BsuRI GGCC 2 cut(s) 41, 1035
BsuTUI ATCGAT 1 cut(s) 885
BtgI CCRYGG 1 cut(s) 324
BtgZI GCGATG 2 cut(s) 317, 393
BtsCI GGATG 4 cut(s) 453, 704, 827, 1028
BtsI GCAGTG 1 cut(s) 226
BtsIMutI CAGTG 3 cut(s) 226, 552, 1219
Cac8I GCNNGC 4 cut(s) 21, 196, 738, 756
CaiI CAGNNNCTG 1 cut(s) 908
Cfr13I GGNCC 5 cut(s) 39, 562, 799, 1034, 1095
ClaI ATCGAT 1 cut(s) 885
CseI GACGC 1 cut(s) 18
CsiI ACCWGGT 1 cut(s) 1314
CviAII CATG 6 cut(s) 20, 126, 171, 195, 325, 507
DdeI CTNAG 2 cut(s) 411, 960
DpnI GATC 6 cut(s) 265, 573, 787, 906, 1031, 1253
DpnII GATC 6 cut(s) 263, 571, 785, 904, 1029, 1251
Eco130I CCWWGG 4 cut(s) 324, 781, 1167, 1323
Eco47I GGWCC 3 cut(s) 562, 799, 1095
Eco57I CTGAAG 1 cut(s) 1397
EcoO109I RGGNCCY 1 cut(s) 562
EcoRII CCWGG 1 cut(s) 1314
EcoT14I CCWWGG 4 cut(s) 324, 781, 1167, 1323
ErhI CCWWGG 4 cut(s) 324, 781, 1167, 1323
FaeI CATG 6 cut(s) 23, 129, 174, 198, 328, 510
FalI AAGNNNNNCTT 5 cut(s) 224, 256, 915, 947, 1397
FaqI GGGAC 3 cut(s) 353, 575, 1108
FatI CATG 6 cut(s) 19, 125, 170, 194, 324, 506
Fnu4HI GCNGC 6 cut(s) 71, 123, 148, 454, 840, 969
FokI GGATG 4 cut(s) 460, 691, 814, 1035
Fsp4HI GCNGC 6 cut(s) 71, 123, 148, 454, 840, 969
FspBI CTAG 9 cut(s) 26, 54, 117, 140, 261, 512, 1073, 1163, 1185
GluI GCNGC 6 cut(s) 71, 123, 148, 454, 840, 969
GsaI CCCAGC 1 cut(s) 202
HaeIII GGCC 2 cut(s) 41, 1035
HapII CCGG 2 cut(s) 74, 1019
HgaI GACGC 1 cut(s) 18
Hin1II CATG 6 cut(s) 23, 129, 174, 198, 328, 510
HindIII AAGCTT 1 cut(s) 929
HinfI GANTC 2 cut(s) 353, 890
HpaII CCGG 2 cut(s) 74, 1019
HphI GGTGA 2 cut(s) 214, 1039
Hpy166II GTNNAC 1 cut(s) 1190
Hpy188I TCNGA 5 cut(s) 904, 1056, 1080, 1228, 1377
Hpy188III TCNNGA 4 cut(s) 140, 261, 560, 1274
Hpy8I GTNNAC 1 cut(s) 1190
HpyAV CCTTC 6 cut(s) 647, 680, 757, 790, 1372, 1393
HpyCH4III ACNGT 8 cut(s) 166, 284, 395, 544, 556, 1066, 1223, 1387
HpyCH4IV ACGT 2 cut(s) 953, 1192
HpyCH4V TGCA 5 cut(s) 231, 529, 983, 1154, 1246
HpyF10VI GCNNNNNNNGC 6 cut(s) 122, 237, 462, 517, 526, 965
HpyF3I CTNAG 2 cut(s) 411, 960
HpySE526I ACGT 2 cut(s) 953, 1192
Hsp92II CATG 6 cut(s) 23, 129, 174, 198, 328, 510
KflI GGGWCCC 1 cut(s) 562
Kzo9I GATC 6 cut(s) 263, 571, 785, 904, 1029, 1251
LmnI GCTCC 2 cut(s) 485, 608
Lsp1109I GCAGC 5 cut(s) 57, 134, 440, 851, 955
LweI GCATC 3 cut(s) 497, 512, 713
MabI ACCWGGT 1 cut(s) 1314
MaeI CTAG 9 cut(s) 26, 54, 117, 140, 261, 512, 1073, 1163, 1185
MaeII ACGT 2 cut(s) 953, 1192
MaeIII GTNAC 4 cut(s) 209, 395, 414, 478
MalI GATC 6 cut(s) 265, 573, 787, 906, 1031, 1253
MboI GATC 6 cut(s) 263, 571, 785, 904, 1029, 1251
MboII GAAGA 7 cut(s) 128, 290, 662, 1246, 1262, 1298, 1405
MflI RGATCY 3 cut(s) 263, 904, 1251
MhlI GDGCHC 1 cut(s) 591
MluCI AATT 4 cut(s) 157, 743, 747, 996
MmeI TCCRAC 1 cut(s) 1186
MroXI GAANNNNTTC 4 cut(s) 621, 1074, 1104, 1412
MseI TTAA 5 cut(s) 315, 746, 774, 852, 1061
MspI CCGG 2 cut(s) 74, 1019
MspR9I CCNGG 2 cut(s) 1020, 1316
Mva1269I GAATGC 1 cut(s) 403
MvaI CCWGG 1 cut(s) 1316
MwoI GCNNNNNNNGC 6 cut(s) 122, 237, 462, 517, 526, 965
NciI CCSGG 1 cut(s) 1020
NcoI CCATGG 1 cut(s) 324
NdeII GATC 6 cut(s) 263, 571, 785, 904, 1029, 1251
NlaIII CATG 6 cut(s) 23, 129, 174, 198, 328, 510
NlaIV GGNNCC 4 cut(s) 286, 563, 564, 1096
NmeAIII GCCGAG 1 cut(s) 29
NmuCI GTSAC 4 cut(s) 209, 395, 414, 478
NspI RCATGY 2 cut(s) 23, 198
PaeI GCATGC 2 cut(s) 23, 198
PctI GAATGC 1 cut(s) 403
PdmI GAANNNNTTC 4 cut(s) 621, 1074, 1104, 1412
PfeI GAWTC 2 cut(s) 353, 890
PflFI GACNNNGTC 1 cut(s) 668
PflMI CCANNNNNTGG 1 cut(s) 1342
PfoI TCCNGGA 1 cut(s) 1018
PkrI GCNGC 6 cut(s) 72, 124, 149, 455, 841, 970
PpuMI RGGWCCY 1 cut(s) 562
PshBI ATTAAT 1 cut(s) 746
PsiI TTATAA 1 cut(s) 824
Psp5II RGGWCCY 1 cut(s) 562
Psp6I CCWGG 1 cut(s) 1314
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 1 cut(s) 1314
PspN4I GGNNCC 4 cut(s) 286, 563, 564, 1096
PspPI GGNCC 5 cut(s) 39, 562, 799, 1034, 1095
PspPPI RGGWCCY 1 cut(s) 562
PstNI CAGNNNCTG 1 cut(s) 908
PsuI RGATCY 3 cut(s) 263, 904, 1251
PsyI GACNNNGTC 1 cut(s) 668
SaqAI TTAA 5 cut(s) 315, 746, 774, 852, 1061
SatI GCNGC 6 cut(s) 71, 123, 148, 454, 840, 969
Sau3AI GATC 6 cut(s) 263, 571, 785, 904, 1029, 1251
Sau96I GGNCC 5 cut(s) 39, 562, 799, 1034, 1095
ScrFI CCNGG 2 cut(s) 1020, 1316
SduI GDGCHC 1 cut(s) 591
SexAI ACCWGGT 1 cut(s) 1314
SfaNI GCATC 3 cut(s) 497, 512, 713
SfcI CTRYAG 2 cut(s) 162, 1120
SinI GGWCC 3 cut(s) 562, 799, 1095
SpeI ACTAGT 1 cut(s) 1072
SphI GCATGC 2 cut(s) 23, 198
Sse9I AATT 4 cut(s) 157, 743, 747, 996
SsiI CCGC 4 cut(s) 147, 456, 734, 1361
SspMI CTAG 9 cut(s) 26, 54, 117, 140, 261, 512, 1073, 1163, 1185
StyD4I CCNGG 2 cut(s) 1018, 1314
StyI CCWWGG 4 cut(s) 324, 781, 1167, 1323
TaaI ACNGT 8 cut(s) 166, 284, 395, 544, 556, 1066, 1223, 1387
TaiI ACGT 2 cut(s) 956, 1195
TaqI TCGA 3 cut(s) 104, 574, 885
TaqII GACCGA 1 cut(s) 1112
TasI AATT 4 cut(s) 157, 743, 747, 996
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 2 cut(s) 353, 890
Tru1I TTAA 5 cut(s) 315, 746, 774, 852, 1061
Tru9I TTAA 5 cut(s) 315, 746, 774, 852, 1061
TscAI CASTG 3 cut(s) 226, 559, 1226
TseFI GTSAC 4 cut(s) 209, 395, 414, 478
TseI GCWGC 5 cut(s) 70, 122, 453, 839, 968
Tsp45I GTSAC 4 cut(s) 209, 395, 414, 478
TspDTI ATGAA 5 cut(s) 159, 487, 614, 686, 903
TspRI CASTG 3 cut(s) 226, 559, 1226
Tth111I GACNNNGTC 1 cut(s) 668
Van91I CCANNNNNTGG 1 cut(s) 1342
VpaK11BI GGWCC 3 cut(s) 562, 799, 1095
VspI ATTAAT 1 cut(s) 746
XbaI TCTAGA 2 cut(s) 139, 260
XceI RCATGY 2 cut(s) 23, 198
XmnI GAANNNNTTC 4 cut(s) 621, 1074, 1104, 1412
XspI CTAG 9 cut(s) 26, 54, 117, 140, 261, 512, 1073, 1163, 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.