Rmu_sc0001164.1_g000020

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Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001164.1
N/A
Physical Location & Seq
Reverse (-)
89262 .. 91030
1769 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 465 bp
atgccactgacccaataccaacccccaaatctcgtccatgacccttggcccttctgctccatcccgtcgcaatctgaaacggcatcgttcacccacgcgcacatcgactggaaggagacgctgactgcgcacatgtccaaggctgacgtgtcgggtctgcggaaggacgaggtcaaggtggaggtggaggacggcaagatccttcaggtcatcggagacagcagcaggttcaagaggcggttcaggctgccggagaatgcgagagttgagcacgtccaggcggcgatggagaacggcgtcctcaacgtcacggtgcccaaggaggaggccaagaagaagcaggatgaggcgatgacgagtgcagtagcagaactggacaagtatgagtacaatgagtgcaatagcgggattggaggagtgcagttaggcgatgacgagtgcagtagcagaattggacgagtatga

Protein Analysis

154

Amino Acids

17.23

Weight (kDa)

5.38

Isoelectric Point (pI)

51.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 36 - 115 4e-15 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 129
Acc36I ACCTGC 1 cut(s) 216
AccB1I GGYRCC 1 cut(s) 313
AccII CGCG 1 cut(s) 98
AciI CCGC 4 cut(s) 160, 238, 281, 405
AclWI GGATC 1 cut(s) 193
AcuI CTGAAG 1 cut(s) 188
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 1 cut(s) 389
AflIII ACRYGT 2 cut(s) 132, 147
AgsI TTSAA 1 cut(s) 232
AjiI CACGTC 2 cut(s) 148, 274
AjnI CCWGG 1 cut(s) 276
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 2 cut(s) 110, 210
AlwI GGATC 1 cut(s) 193
AoxI GGCC 2 cut(s) 47, 327
ApeKI GCWGC 2 cut(s) 222, 247
AspLEI GCGC 2 cut(s) 100, 130
AspS9I GGNCC 1 cut(s) 48
AsuHPI GGTGA 1 cut(s) 82
BaeGI GKGCMC 1 cut(s) 318
BanI GGYRCC 1 cut(s) 313
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 2 cut(s) 234, 234
BccI CCATC 2 cut(s) 68, 280
BceAI ACGGC 3 cut(s) 96, 208, 310
BciT130I CCWGG 1 cut(s) 278
BcoDI GTCTC 2 cut(s) 110, 210
BfuAI ACCTGC 1 cut(s) 216
BisI GCNGC 3 cut(s) 223, 248, 282
BlsI GCNGC 3 cut(s) 224, 249, 283
Bme1390I CCNGG 1 cut(s) 278
BmgBI CACGTC 2 cut(s) 148, 274
BmgT120I GGNCC 1 cut(s) 48
BmiI GGNNCC 1 cut(s) 315
BmrFI CCNGG 1 cut(s) 278
BmsI GCATC 1 cut(s) 92
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 3 cut(s) 44, 138, 318
Bse1I ACTGG 2 cut(s) 113, 378
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 3 cut(s) 44, 138, 318
BseGI GGATG 2 cut(s) 60, 349
BseNI ACTGG 2 cut(s) 113, 378
BseRI GAGGAG 2 cut(s) 338, 429
BseSI GKGCMC 1 cut(s) 318
BseXI GCAGC 2 cut(s) 234, 234
BsgI GTGCAG 3 cut(s) 381, 440, 460
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 2 cut(s) 49, 329
BshNI GGYRCC 1 cut(s) 313
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 1 cut(s) 251
BsmAI GTCTC 2 cut(s) 110, 210
BsmBI CGTCTC 1 cut(s) 110
BsmI GAATGC 1 cut(s) 262
BsnI GGCC 2 cut(s) 49, 329
Bsp1286I GDGCHC 2 cut(s) 273, 318
Bsp143I GATC 1 cut(s) 198
BspACI CCGC 4 cut(s) 160, 238, 281, 405
BspANI GGCC 2 cut(s) 49, 329
BspFNI CGCG 1 cut(s) 98
BspLI GGNNCC 1 cut(s) 315
BspMI ACCTGC 1 cut(s) 216
BspPI GGATC 1 cut(s) 193
BspT107I GGYRCC 1 cut(s) 313
BsrI ACTGG 2 cut(s) 113, 378
BssECI CCNNGG 3 cut(s) 44, 138, 318
BssMI GATC 1 cut(s) 198
BssNI GRCGYC 1 cut(s) 297
BssT1I CCWWGG 3 cut(s) 44, 138, 318
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 1 cut(s) 313
BstACI GRCGYC 1 cut(s) 297
BstF5I GGATG 2 cut(s) 60, 349
BstFNI CGCG 1 cut(s) 98
BstHHI GCGC 2 cut(s) 100, 130
BstKTI GATC 1 cut(s) 201
BstMAI GTCTC 2 cut(s) 110, 210
BstMBI GATC 1 cut(s) 198
BstMWI GCNNNNNNNGC 2 cut(s) 127, 244
BstNI CCWGG 1 cut(s) 278
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 1 cut(s) 276
BstSLI GKGCMC 1 cut(s) 318
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 2 cut(s) 234, 234
BstX2I RGATCY 1 cut(s) 198
BstYI RGATCY 1 cut(s) 198
BsuRI GGCC 2 cut(s) 49, 329
BtgZI GCGATG 3 cut(s) 299, 365, 444
BtrI CACGTC 2 cut(s) 148, 274
BtsCI GGATG 2 cut(s) 60, 349
BtsIMutI CAGTG 1 cut(s) 5
BveI ACCTGC 1 cut(s) 216
CfoI GCGC 2 cut(s) 100, 130
Cfr13I GGNCC 1 cut(s) 48
CseI GACGC 2 cut(s) 127, 286
Csp6I GTAC 1 cut(s) 388
CviAII CATG 2 cut(s) 38, 133
CviJI RGCY 4 cut(s) 49, 143, 247, 329
CviKI_1 RGCY 4 cut(s) 49, 143, 247, 329
CviQI GTAC 1 cut(s) 388
DpnI GATC 1 cut(s) 200
DpnII GATC 1 cut(s) 198
Eco130I CCWWGG 3 cut(s) 44, 138, 318
Eco57I CTGAAG 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 3 cut(s) 44, 138, 318
ErhI CCWWGG 3 cut(s) 44, 138, 318
Esp3I CGTCTC 1 cut(s) 110
FaeI CATG 2 cut(s) 41, 136
FaiI YATR 4 cut(s) 39, 134, 384, 463
FatI CATG 2 cut(s) 37, 132
FauI CCCGC 1 cut(s) 398
Fnu4HI GCNGC 3 cut(s) 223, 248, 282
FokI GGATG 2 cut(s) 47, 356
Fsp4HI GCNGC 3 cut(s) 223, 248, 282
FspI TGCGCA 1 cut(s) 129
GlaI GCGC 2 cut(s) 99, 129
GluI GCNGC 3 cut(s) 223, 248, 282
HaeIII GGCC 2 cut(s) 49, 329
HapII CCGG 1 cut(s) 251
HgaI GACGC 2 cut(s) 127, 286
HhaI GCGC 2 cut(s) 100, 130
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 2 cut(s) 41, 136
Hin6I GCGC 2 cut(s) 98, 128
HinP1I GCGC 2 cut(s) 98, 128
HpaII CCGG 1 cut(s) 251
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 1 cut(s) 90
Hpy188I TCNGA 2 cut(s) 76, 215
Hpy188III TCNNGA 1 cut(s) 232
Hpy8I GTNNAC 1 cut(s) 90
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 4 cut(s) 61, 106, 157, 212
HpyCH4III ACNGT 1 cut(s) 313
HpyCH4IV ACGT 3 cut(s) 147, 273, 306
HpyCH4V TGCA 4 cut(s) 362, 399, 421, 441
HpyF10VI GCNNNNNNNGC 2 cut(s) 127, 244
HpySE526I ACGT 3 cut(s) 147, 273, 306
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 2 cut(s) 41, 136
HspAI GCGC 2 cut(s) 98, 128
Kzo9I GATC 1 cut(s) 198
LmnI GCTCC 1 cut(s) 62
LpnPI CCDG 9 cut(s) 94, 191, 211, 229, 263, 264, 290, 326, 359
Lsp1109I GCAGC 2 cut(s) 234, 234
LweI GCATC 1 cut(s) 92
MaeII ACGT 3 cut(s) 147, 273, 306
MaeIII GTNAC 1 cut(s) 307
MalI GATC 1 cut(s) 200
MboI GATC 1 cut(s) 198
MboII GAAGA 1 cut(s) 346
MflI RGATCY 1 cut(s) 198
MhlI GDGCHC 2 cut(s) 273, 318
MluCI AATT 1 cut(s) 450
MnlI CCTC 9 cut(s) 163, 175, 181, 228, 311, 316, 319, 340, 407
MspI CCGG 1 cut(s) 251
MspR9I CCNGG 1 cut(s) 278
Mva1269I GAATGC 1 cut(s) 262
MvaI CCWGG 1 cut(s) 278
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 2 cut(s) 127, 244
NdeII GATC 1 cut(s) 198
NlaIII CATG 2 cut(s) 41, 136
NlaIV GGNNCC 1 cut(s) 315
NmuCI GTSAC 1 cut(s) 307
NsbI TGCGCA 1 cut(s) 129
NspI RCATGY 1 cut(s) 136
PciI ACATGT 1 cut(s) 132
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 1 cut(s) 262
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 3 cut(s) 224, 249, 283
PscI ACATGT 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 1 cut(s) 315
PspPI GGNCC 1 cut(s) 48
PsuI RGATCY 1 cut(s) 198
PsyI GACNNNGTC 1 cut(s) 170
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
SatI GCNGC 3 cut(s) 223, 248, 282
Sau3AI GATC 1 cut(s) 198
Sau96I GGNCC 1 cut(s) 48
ScrFI CCNGG 1 cut(s) 278
SduI GDGCHC 2 cut(s) 273, 318
SetI ASST 8 cut(s) 150, 174, 180, 186, 210, 230, 276, 309
SfaNI GCATC 1 cut(s) 92
Sse9I AATT 1 cut(s) 450
SsiI CCGC 4 cut(s) 160, 238, 281, 405
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 3 cut(s) 44, 138, 318
TaaI ACNGT 1 cut(s) 313
TaiI ACGT 3 cut(s) 150, 276, 309
TaqI TCGA 1 cut(s) 105
TasI AATT 1 cut(s) 450
TatI WGTACW 1 cut(s) 387
TauI GCSGC 1 cut(s) 284
TscAI CASTG 1 cut(s) 12
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 2 cut(s) 222, 247
Tsp45I GTSAC 1 cut(s) 307
TspRI CASTG 1 cut(s) 12
Tth111I GACNNNGTC 1 cut(s) 170
XceI RCATGY 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.