The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
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No orthologs found for this gene in the current database.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 506 |
| AatII | GACGTC | 3 cut(s) | 90, 393, 2021 |
| AccII | CGCG | 2 cut(s) | 639, 896 |
| AclWI | GGATC | 6 cut(s) | 60, 88, 185, 257, 577, 1763 |
| AcoI | YGGCCR | 3 cut(s) | 98, 228, 1878 |
| AcsI | RAATTY | 1 cut(s) | 1861 |
| AcuI | CTGAAG | 3 cut(s) | 1733, 1803, 1841 |
| AcyI | GRCGYC | 5 cut(s) | 87, 155, 390, 642, 2018 |
| AfaI | GTAC | 4 cut(s) | 632, 1028, 2153, 2191 |
| AfeI | AGCGCT | 1 cut(s) | 292 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 116, 496, 565, 1846 |
| AflII | CTTAAG | 1 cut(s) | 1214 |
| AgsI | TTSAA | 7 cut(s) | 670, 1366, 1568, 1638, 1676, 2138, 2449 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 119, 154 |
| AjnI | CCWGG | 2 cut(s) | 1333, 2175 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1853, 1885 |
| Alw21I | GWGCWC | 4 cut(s) | 726, 1263, 1481, 2164 |
| Alw26I | GTCTC | 2 cut(s) | 1975, 2009 |
| AlwI | GGATC | 6 cut(s) | 60, 88, 185, 257, 577, 1763 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 802, 1429 |
| Ama87I | CYCGRG | 2 cut(s) | 533, 558 |
| Aor51HI | AGCGCT | 1 cut(s) | 292 |
| AoxI | GGCC | 9 cut(s) | 98, 228, 348, 675, 837, 1266, 1524, 1878, 2461 |
| ApeKI | GCWGC | 5 cut(s) | 80, 319, 958, 1136, 2297 |
| ApoI | RAATTY | 1 cut(s) | 1861 |
| AseI | ATTAAT | 1 cut(s) | 1206 |
| AspLEI | GCGC | 6 cut(s) | 223, 293, 761, 814, 896, 898 |
| AspS9I | GGNCC | 6 cut(s) | 325, 349, 499, 1266, 1486, 2462 |
| AsuC2I | CCSGG | 1 cut(s) | 575 |
| AsuHPI | GGTGA | 6 cut(s) | 167, 460, 803, 1025, 2353, 2434 |
| AvaI | CYCGRG | 2 cut(s) | 533, 558 |
| AvaII | GGWCC | 3 cut(s) | 325, 499, 1486 |
| BaeGI | GKGCMC | 3 cut(s) | 35, 61, 344 |
| BalI | TGGCCA | 1 cut(s) | 1880 |
| BanII | GRGCYC | 1 cut(s) | 623 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1010, 1042 |
| BauI | CACGAG | 1 cut(s) | 1097 |
| BbsI | GAAGAC | 1 cut(s) | 1307 |
| Bbv12I | GWGCWC | 4 cut(s) | 726, 1263, 1481, 2164 |
| BbvCI | CCTCAGC | 3 cut(s) | 13, 287, 1528 |
| BbvI | GCAGC | 5 cut(s) | 67, 306, 970, 1148, 2284 |
| BccI | CCATC | 8 cut(s) | 237, 961, 1492, 1497, 1709, 1879, 2272, 2363 |
| BceAI | ACGGC | 5 cut(s) | 89, 457, 676, 737, 1253 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 642, 676 |
| BciT130I | CCWGG | 2 cut(s) | 1335, 2177 |
| BciVI | GTATCC | 3 cut(s) | 1507, 1606, 2378 |
| BcnI | CCSGG | 1 cut(s) | 575 |
| BcoDI | GTCTC | 2 cut(s) | 1975, 2009 |
| BfaI | CTAG | 1 cut(s) | 1928 |
| BfoI | RGCGCY | 1 cut(s) | 294 |
| BfrI | CTTAAG | 1 cut(s) | 1214 |
| BfuI | GTATCC | 3 cut(s) | 1507, 1606, 2378 |
| BisI | GCNGC | 9 cut(s) | 45, 81, 101, 320, 640, 959, 1137, 2298, 2461 |
| BlpI | GCTNAGC | 2 cut(s) | 954, 1395 |
| BlsI | GCNGC | 9 cut(s) | 46, 82, 102, 321, 641, 960, 1138, 2299, 2462 |
| BmcAI | AGTACT | 1 cut(s) | 2191 |
| Bme1390I | CCNGG | 3 cut(s) | 575, 1335, 2177 |
| Bme18I | GGWCC | 3 cut(s) | 325, 499, 1486 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 119, 154 |
| BmeT110I | CYCGRG | 2 cut(s) | 533, 558 |
| BmgT120I | GGNCC | 6 cut(s) | 325, 349, 499, 1266, 1486, 2462 |
| BmrFI | CCNGG | 3 cut(s) | 575, 1335, 2177 |
| BmsI | GCATC | 5 cut(s) | 799, 1123, 1416, 1714, 2323 |
| BpiI | GAAGAC | 1 cut(s) | 1307 |
| BpmI | CTGGAG | 5 cut(s) | 599, 740, 947, 1719, 1917 |
| Bpu10I | CCTNAGC | 4 cut(s) | 13, 287, 1262, 1528 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 954, 1395 |
| BpuEI | CTTGAG | 4 cut(s) | 1421, 1676, 2051, 2394 |
| BpuMI | CCSGG | 1 cut(s) | 575 |
| Bsa29I | ATCGAT | 1 cut(s) | 825 |
| BsaBI | GATNNNNATC | 2 cut(s) | 587, 1518 |
| BsaHI | GRCGYC | 5 cut(s) | 87, 155, 390, 642, 2018 |
| BsaI | GGTCTC | 1 cut(s) | 1975 |
| BsaJI | CCNNGG | 6 cut(s) | 495, 559, 637, 1334, 1845, 2175 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1692, 1722, 1915, 1945 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 116, 496, 565, 1846 |
| Bse118I | RCCGGY | 2 cut(s) | 346, 663 |
| Bse1I | ACTGG | 3 cut(s) | 1065, 1702, 2277 |
| Bse3DI | GCAATG | 2 cut(s) | 201, 1046 |
| Bse8I | GATNNNNATC | 2 cut(s) | 587, 1518 |
| BseBI | CCWGG | 2 cut(s) | 1335, 2177 |
| BseCI | ATCGAT | 1 cut(s) | 825 |
| BseDI | CCNNGG | 6 cut(s) | 495, 559, 637, 1334, 1845, 2175 |
| BseGI | GGATG | 8 cut(s) | 633, 814, 1261, 1489, 1543, 1720, 2058, 2338 |
| BseJI | GATNNNNATC | 2 cut(s) | 587, 1518 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 116, 496, 565, 1846 |
| BseMI | GCAATG | 2 cut(s) | 201, 1046 |
| BseNI | ACTGG | 3 cut(s) | 1065, 1702, 2277 |
| BsePI | GCGCGC | 1 cut(s) | 894 |
| BseRI | GAGGAG | 3 cut(s) | 512, 515, 1963 |
| BseSI | GKGCMC | 3 cut(s) | 35, 61, 344 |
| BseX3I | CGGCCG | 1 cut(s) | 98 |
| BseXI | GCAGC | 5 cut(s) | 67, 306, 970, 1148, 2284 |
| BseYI | CCCAGC | 1 cut(s) | 456 |
| BsgI | GTGCAG | 1 cut(s) | 1473 |
| Bsh1236I | CGCG | 2 cut(s) | 639, 896 |
| Bsh1285I | CGRYCG | 2 cut(s) | 101, 829 |
| BshFI | GGCC | 9 cut(s) | 100, 230, 350, 677, 839, 1268, 1526, 1880, 2463 |
| BshVI | ATCGAT | 1 cut(s) | 825 |
| BsiEI | CGRYCG | 2 cut(s) | 101, 829 |
| BsiHKAI | GWGCWC | 4 cut(s) | 726, 1263, 1481, 2164 |
| BsiHKCI | CYCGRG | 2 cut(s) | 533, 558 |
| BsiSI | CCGG | 7 cut(s) | 97, 124, 227, 347, 575, 664, 815 |
| BslFI | GGGAC | 2 cut(s) | 18, 2447 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 116, 496, 565, 1846 |
| BsmAI | GTCTC | 2 cut(s) | 1975, 2009 |
| BsmBI | CGTCTC | 1 cut(s) | 2009 |
| BsmFI | GGGAC | 2 cut(s) | 18, 2447 |
| BsmI | GAATGC | 2 cut(s) | 223, 1743 |
| BsnI | GGCC | 9 cut(s) | 100, 230, 350, 677, 839, 1268, 1526, 1880, 2463 |
| Bso31I | GGTCTC | 1 cut(s) | 1975 |
| BsoBI | CYCGRG | 2 cut(s) | 533, 558 |
| Bsp1286I | GDGCHC | 8 cut(s) | 35, 61, 344, 623, 726, 1263, 1481, 2164 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 954, 1395 |
| BspANI | GGCC | 9 cut(s) | 100, 230, 350, 677, 839, 1268, 1526, 1880, 2463 |
| BspDI | ATCGAT | 1 cut(s) | 825 |
| BspFNI | CGCG | 2 cut(s) | 639, 896 |
| BspHI | TCATGA | 1 cut(s) | 426 |
| BspPI | GGATC | 6 cut(s) | 60, 88, 185, 257, 577, 1763 |
| BspQI | GCTCTTC | 1 cut(s) | 467 |
| BspTI | CTTAAG | 1 cut(s) | 1214 |
| BspTNI | GGTCTC | 1 cut(s) | 1975 |
| BsrDI | GCAATG | 2 cut(s) | 201, 1046 |
| BsrFI | RCCGGY | 2 cut(s) | 346, 663 |
| BsrI | ACTGG | 3 cut(s) | 1065, 1702, 2277 |
| BssAI | RCCGGY | 2 cut(s) | 346, 663 |
| BssECI | CCNNGG | 6 cut(s) | 495, 559, 637, 1334, 1845, 2175 |
| BssHII | GCGCGC | 1 cut(s) | 894 |
| BssNI | GRCGYC | 5 cut(s) | 87, 155, 390, 642, 2018 |
| BssSI | CACGAG | 1 cut(s) | 1097 |
| BssT1I | CCWWGG | 1 cut(s) | 1845 |
| Bst2BI | CACGAG | 1 cut(s) | 1097 |
| Bst2UI | CCWGG | 2 cut(s) | 1335, 2177 |
| Bst4CI | ACNGT | 7 cut(s) | 508, 1151, 1483, 1798, 1831, 2041, 2194 |
| Bst6I | CTCTTC | 2 cut(s) | 68, 467 |
| BstACI | GRCGYC | 5 cut(s) | 87, 155, 390, 642, 2018 |
| BstAFI | CTTAAG | 1 cut(s) | 1214 |
| BstC8I | GCNNGC | 9 cut(s) | 78, 208, 317, 344, 348, 896, 1437, 1477, 1652 |
| BstDSI | CCRYGG | 1 cut(s) | 637 |
| BstF5I | GGATG | 8 cut(s) | 633, 814, 1261, 1489, 1543, 1720, 2058, 2338 |
| BstFNI | CGCG | 2 cut(s) | 639, 896 |
| BstH2I | RGCGCY | 1 cut(s) | 294 |
| BstHHI | GCGC | 6 cut(s) | 223, 293, 761, 814, 896, 898 |
| BstMAI | GTCTC | 2 cut(s) | 1975, 2009 |
| BstMCI | CGRYCG | 2 cut(s) | 101, 829 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 227, 288, 758, 793, 1121 |
| BstNI | CCWGG | 2 cut(s) | 1335, 2177 |
| BstSCI | CCNGG | 3 cut(s) | 573, 1333, 2175 |
| BstSLI | GKGCMC | 3 cut(s) | 35, 61, 344 |
| BstUI | CGCG | 2 cut(s) | 639, 896 |
| BstV1I | GCAGC | 5 cut(s) | 67, 306, 970, 1148, 2284 |
| BstV2I | GAAGAC | 1 cut(s) | 1307 |
| BstX2I | RGATCY | 2 cut(s) | 93, 249 |
| BstYI | RGATCY | 2 cut(s) | 93, 249 |
| BstZI | CGGCCG | 1 cut(s) | 98 |
| Bsu15I | ATCGAT | 1 cut(s) | 825 |
| BsuI | GTATCC | 3 cut(s) | 1507, 1606, 2378 |
| BsuRI | GGCC | 9 cut(s) | 100, 230, 350, 677, 839, 1268, 1526, 1880, 2463 |
| BsuTUI | ATCGAT | 1 cut(s) | 825 |
| BtgI | CCRYGG | 1 cut(s) | 637 |
| BtsCI | GGATG | 8 cut(s) | 633, 814, 1261, 1489, 1543, 1720, 2058, 2338 |
| BtsI | GCAGTG | 2 cut(s) | 277, 1119 |
| BtsIMutI | CAGTG | 5 cut(s) | 277, 1072, 1119, 2270, 2420 |
| Cac8I | GCNNGC | 9 cut(s) | 78, 208, 317, 344, 348, 896, 1437, 1477, 1652 |
| CaiI | CAGNNNCTG | 2 cut(s) | 802, 1429 |
| CciI | TCATGA | 1 cut(s) | 426 |
| CfoI | GCGC | 6 cut(s) | 223, 293, 761, 814, 896, 898 |
| Cfr10I | RCCGGY | 2 cut(s) | 346, 663 |
| Cfr13I | GGNCC | 6 cut(s) | 325, 349, 499, 1266, 1486, 2462 |
| Cfr42I | CCGCGG | 1 cut(s) | 640 |
| ClaI | ATCGAT | 1 cut(s) | 825 |
| CseI | GACGC | 4 cut(s) | 144, 194, 631, 1898 |
| Csp6I | GTAC | 4 cut(s) | 631, 1027, 2152, 2190 |
| CviQI | GTAC | 4 cut(s) | 631, 1027, 2152, 2190 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 506 |
| DriI | GACNNNNNGTC | 2 cut(s) | 119, 154 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 506 |
| EaeI | YGGCCR | 3 cut(s) | 98, 228, 1878 |
| EagI | CGGCCG | 1 cut(s) | 98 |
| Eam1104I | CTCTTC | 2 cut(s) | 68, 467 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 119, 154 |
| EarI | CTCTTC | 2 cut(s) | 68, 467 |
| EciI | GGCGGA | 1 cut(s) | 121 |
| EclXI | CGGCCG | 1 cut(s) | 98 |
| Eco130I | CCWWGG | 1 cut(s) | 1845 |
| Eco24I | GRGCYC | 1 cut(s) | 623 |
| Eco31I | GGTCTC | 1 cut(s) | 1975 |
| Eco47I | GGWCC | 3 cut(s) | 325, 499, 1486 |
| Eco47III | AGCGCT | 1 cut(s) | 292 |
| Eco52I | CGGCCG | 1 cut(s) | 98 |
| Eco57I | CTGAAG | 3 cut(s) | 1733, 1803, 1841 |
| Eco88I | CYCGRG | 2 cut(s) | 533, 558 |
| EcoRII | CCWGG | 2 cut(s) | 1333, 2175 |
| EcoT14I | CCWWGG | 1 cut(s) | 1845 |
| EcoT38I | GRGCYC | 1 cut(s) | 623 |
| ErhI | CCWWGG | 1 cut(s) | 1845 |
| Esp3I | CGTCTC | 1 cut(s) | 2009 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 60, 92, 2429, 2461 |
| FaqI | GGGAC | 2 cut(s) | 18, 2447 |
| FauI | CCCGC | 1 cut(s) | 215 |
| Fnu4HI | GCNGC | 9 cut(s) | 45, 81, 101, 320, 640, 959, 1137, 2298, 2461 |
| FokI | GGATG | 8 cut(s) | 620, 821, 1268, 1476, 1550, 1727, 2045, 2345 |
| FriOI | GRGCYC | 1 cut(s) | 623 |
| Fsp4HI | GCNGC | 9 cut(s) | 45, 81, 101, 320, 640, 959, 1137, 2298, 2461 |
| FspBI | CTAG | 1 cut(s) | 1928 |
| GlaI | GCGC | 6 cut(s) | 222, 292, 760, 813, 895, 897 |
| GluI | GCNGC | 9 cut(s) | 45, 81, 101, 320, 640, 959, 1137, 2298, 2461 |
| GsaI | CCCAGC | 1 cut(s) | 460 |
| GsuI | CTGGAG | 5 cut(s) | 599, 740, 947, 1719, 1917 |
| HaeII | RGCGCY | 1 cut(s) | 294 |
| HaeIII | GGCC | 9 cut(s) | 100, 230, 350, 677, 839, 1268, 1526, 1880, 2463 |
| HapII | CCGG | 7 cut(s) | 97, 124, 227, 347, 575, 664, 815 |
| HgaI | GACGC | 4 cut(s) | 144, 194, 631, 1898 |
| HhaI | GCGC | 6 cut(s) | 223, 293, 761, 814, 896, 898 |
| Hin1I | GRCGYC | 5 cut(s) | 87, 155, 390, 642, 2018 |
| Hin6I | GCGC | 6 cut(s) | 221, 291, 759, 812, 894, 896 |
| HinP1I | GCGC | 6 cut(s) | 221, 291, 759, 812, 894, 896 |
| HindIII | AAGCTT | 1 cut(s) | 515 |
| HpaII | CCGG | 7 cut(s) | 97, 124, 227, 347, 575, 664, 815 |
| HphI | GGTGA | 6 cut(s) | 167, 460, 803, 1025, 2353, 2434 |
| Hpy166II | GTNNAC | 3 cut(s) | 328, 924, 1078 |
| Hpy188I | TCNGA | 8 cut(s) | 496, 653, 844, 1860, 1917, 2118, 2220, 2393 |
| Hpy8I | GTNNAC | 3 cut(s) | 328, 924, 1078 |
| HpyCH4III | ACNGT | 7 cut(s) | 508, 1151, 1483, 1798, 1831, 2041, 2194 |
| HpyCH4IV | ACGT | 6 cut(s) | 87, 390, 483, 1690, 2018, 2154 |
| HpyCH4V | TGCA | 8 cut(s) | 315, 1124, 1136, 1435, 1454, 1727, 2312, 2420 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 227, 288, 758, 793, 1121 |
| HpySE526I | ACGT | 6 cut(s) | 87, 390, 483, 1690, 2018, 2154 |
| Hsp92I | GRCGYC | 5 cut(s) | 87, 155, 390, 642, 2018 |
| HspAI | GCGC | 6 cut(s) | 221, 291, 759, 812, 894, 896 |
| KroI | GCCGGC | 1 cut(s) | 346 |
| KroNI | GCCGGC | 1 cut(s) | 348 |
| KspI | CCGCGG | 1 cut(s) | 640 |
| LguI | GCTCTTC | 1 cut(s) | 467 |
| LmnI | GCTCC | 5 cut(s) | 287, 654, 721, 966, 1936 |
| Lsp1109I | GCAGC | 5 cut(s) | 67, 306, 970, 1148, 2284 |
| LweI | GCATC | 5 cut(s) | 799, 1123, 1416, 1714, 2323 |
| MaeI | CTAG | 1 cut(s) | 1928 |
| MaeII | ACGT | 6 cut(s) | 87, 390, 483, 1690, 2018, 2154 |
| MaeIII | GTNAC | 5 cut(s) | 643, 1161, 1792, 1825, 2000 |
| MflI | RGATCY | 2 cut(s) | 93, 249 |
| MhlI | GDGCHC | 8 cut(s) | 35, 61, 344, 623, 726, 1263, 1481, 2164 |
| MlsI | TGGCCA | 1 cut(s) | 1880 |
| MluCI | AATT | 7 cut(s) | 450, 889, 980, 1125, 1596, 1861, 2394 |
| MluNI | TGGCCA | 1 cut(s) | 1880 |
| MlyI | GAGTC | 8 cut(s) | 129, 1285, 1297, 1378, 2251, 2298, 2401, 2434 |
| MmeI | TCCRAC | 3 cut(s) | 957, 1590, 1641 |
| Mox20I | TGGCCA | 1 cut(s) | 1880 |
| MroNI | GCCGGC | 1 cut(s) | 346 |
| MscI | TGGCCA | 1 cut(s) | 1880 |
| MslI | CAYNNNNRTG | 1 cut(s) | 770 |
| Msp20I | TGGCCA | 1 cut(s) | 1880 |
| MspA1I | CMGCKG | 2 cut(s) | 17, 639 |
| MspCI | CTTAAG | 1 cut(s) | 1214 |
| MspI | CCGG | 7 cut(s) | 97, 124, 227, 347, 575, 664, 815 |
| MspR9I | CCNGG | 3 cut(s) | 575, 1335, 2177 |
| Mva1269I | GAATGC | 2 cut(s) | 223, 1743 |
| MvaI | CCWGG | 2 cut(s) | 1335, 2177 |
| MvnI | CGCG | 2 cut(s) | 639, 896 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 227, 288, 758, 793, 1121 |
| NaeI | GCCGGC | 1 cut(s) | 348 |
| NciI | CCSGG | 1 cut(s) | 575 |
| NgoMIV | GCCGGC | 1 cut(s) | 346 |
| NmeAIII | GCCGAG | 2 cut(s) | 160, 703 |
| NmuCI | GTSAC | 2 cut(s) | 643, 1161 |
| PaeR7I | CTCGAG | 1 cut(s) | 533 |
| PagI | TCATGA | 1 cut(s) | 426 |
| PauI | GCGCGC | 1 cut(s) | 894 |
| PciSI | GCTCTTC | 1 cut(s) | 467 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 710, 1827 |
| PctI | GAATGC | 2 cut(s) | 223, 1743 |
| PdiI | GCCGGC | 1 cut(s) | 348 |
| PfeI | GAWTC | 8 cut(s) | 554, 654, 697, 706, 715, 1761, 1937, 2221 |
| PfoI | TCCNGGA | 1 cut(s) | 573 |
| PkrI | GCNGC | 9 cut(s) | 46, 82, 102, 321, 641, 960, 1138, 2299, 2462 |
| Ple19I | CGATCG | 1 cut(s) | 829 |
| PleI | GAGTC | 8 cut(s) | 128, 1284, 1297, 1378, 2251, 2297, 2401, 2433 |
| PpsI | GAGTC | 8 cut(s) | 128, 1284, 1297, 1378, 2251, 2297, 2401, 2433 |
| PshBI | ATTAAT | 1 cut(s) | 1206 |
| Psp6I | CCWGG | 2 cut(s) | 1333, 2175 |
| PspFI | CCCAGC | 1 cut(s) | 456 |
| PspGI | CCWGG | 2 cut(s) | 1333, 2175 |
| PspPI | GGNCC | 6 cut(s) | 325, 349, 499, 1266, 1486, 2462 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1400, 1432 |
| PstNI | CAGNNNCTG | 2 cut(s) | 802, 1429 |
| PsuI | RGATCY | 2 cut(s) | 93, 249 |
| PteI | GCGCGC | 1 cut(s) | 894 |
| PvuI | CGATCG | 1 cut(s) | 829 |
| PvuII | CAGCTG | 1 cut(s) | 17 |
| RsaI | GTAC | 4 cut(s) | 632, 1028, 2153, 2191 |
| RsaNI | GTAC | 4 cut(s) | 631, 1027, 2152, 2190 |
| RseI | CAYNNNNRTG | 1 cut(s) | 770 |
| SacII | CCGCGG | 1 cut(s) | 640 |
| SapI | GCTCTTC | 1 cut(s) | 467 |
| SatI | GCNGC | 9 cut(s) | 45, 81, 101, 320, 640, 959, 1137, 2298, 2461 |
| Sau96I | GGNCC | 6 cut(s) | 325, 349, 499, 1266, 1486, 2462 |
| ScaI | AGTACT | 1 cut(s) | 2191 |
| SchI | GAGTC | 8 cut(s) | 129, 1285, 1297, 1378, 2251, 2298, 2401, 2434 |
| ScrFI | CCNGG | 3 cut(s) | 575, 1335, 2177 |
| SduI | GDGCHC | 8 cut(s) | 35, 61, 344, 623, 726, 1263, 1481, 2164 |
| SfaNI | GCATC | 5 cut(s) | 799, 1123, 1416, 1714, 2323 |
| Sfr274I | CTCGAG | 1 cut(s) | 533 |
| Sfr303I | CCGCGG | 1 cut(s) | 640 |
| SgrBI | CCGCGG | 1 cut(s) | 640 |
| SinI | GGWCC | 3 cut(s) | 325, 499, 1486 |
| SlaI | CTCGAG | 1 cut(s) | 533 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 770 |
| SmlI | CTYRAG | 6 cut(s) | 533, 1214, 1400, 1655, 2030, 2409 |
| SmoI | CTYRAG | 6 cut(s) | 533, 1214, 1400, 1655, 2030, 2409 |
| Sse9I | AATT | 7 cut(s) | 450, 889, 980, 1125, 1596, 1861, 2394 |
| SspI | AATATT | 1 cut(s) | 1342 |
| SspMI | CTAG | 1 cut(s) | 1928 |
| StyD4I | CCNGG | 3 cut(s) | 573, 1333, 2175 |
| StyI | CCWWGG | 1 cut(s) | 1845 |
| TaaI | ACNGT | 7 cut(s) | 508, 1151, 1483, 1798, 1831, 2041, 2194 |
| TaiI | ACGT | 6 cut(s) | 90, 393, 486, 1693, 2021, 2157 |
| TasI | AATT | 7 cut(s) | 450, 889, 980, 1125, 1596, 1861, 2394 |
| TatI | WGTACW | 3 cut(s) | 630, 1026, 2189 |
| TauI | GCSGC | 4 cut(s) | 47, 103, 642, 2463 |
| TfiI | GAWTC | 8 cut(s) | 554, 654, 697, 706, 715, 1761, 1937, 2221 |
| TscAI | CASTG | 5 cut(s) | 284, 1072, 1126, 2277, 2427 |
| TseFI | GTSAC | 2 cut(s) | 643, 1161 |
| TseI | GCWGC | 5 cut(s) | 80, 319, 958, 1136, 2297 |
| Tsp45I | GTSAC | 2 cut(s) | 643, 1161 |
| TspDTI | ATGAA | 6 cut(s) | 259, 428, 454, 1437, 2085, 2261 |
| TspGWI | ACGGA | 4 cut(s) | 65, 690, 760, 1645 |
| TspRI | CASTG | 5 cut(s) | 284, 1072, 1126, 2277, 2427 |
| Vha464I | CTTAAG | 1 cut(s) | 1214 |
| VpaK11BI | GGWCC | 3 cut(s) | 325, 499, 1486 |
| VspI | ATTAAT | 1 cut(s) | 1206 |
| XapI | RAATTY | 1 cut(s) | 1861 |
| XhoI | CTCGAG | 1 cut(s) | 533 |
| XspI | CTAG | 1 cut(s) | 1928 |
| ZraI | GACGTC | 3 cut(s) | 88, 391, 2019 |
| ZrmI | AGTACT | 1 cut(s) | 2191 |