Rmu_sc0001181.1_g000005

tryptophan synthase beta chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001181.1
N/A
Physical Location & Seq
Reverse (-)
27726 .. 32335
4610 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2934 bp
atggctactcactgtgttaatttatgtgcaagttcatttgatactaagccttcttcatcaggatcacactccagagttgagaagaaatggcttggatctattgcacttgatagaaagccgaagaatttgaagctttcaaatggctgtagagcaagagcagccctcaatgctgattcaataccaatcgaaatccctcgtcagtggtacaacctgattgcagatcttcctataaaacctcctccttcattgcatcccaagacttttgaaccagtaaaacctgaagatctgtcccctctatttcctgatgagttgattaaacaggaagcaagtggtgaaagatacatagatatcccagaagaagttcgagagatatacaagctttggcgtccaacccctctcattagggcaaagaggttggagaagcttcttgacacgcctgccagaatttattacaagtatgaaggtgttagccctgctggatcacacaaacccaacactgcagttcctcaagtgtattataatgcacaacaaggcatcaagaaggttgtgacagaaacaggagctggtcaatggggatgttctttggcttttgcatccagcttattcggtcttgaatgtgaggtatggcaagtgcgtgcttcgtatgatcagaaaccatatcgcaaattgatgatgcaaacttggggggcaaaggtacacccgtctccatcgaacataactgaatcaggtagaaaaatccttctgatggatccatcgaccccgggaagtttaggaatagctatttctgaagctgtggaggttgcagctatgaatgctgacactaaatactgtctgggaagtgttctcaatcatgttttgctccaccagactgtaataggtgaagagtgtctgaaacaaatggaagcgataggtgaaaccccggacttgatcatagggtgtactggtggaggatccaactttgcagggctaagtttcccattcattagagaaaagctcgatgggaaaatgagccctgtaattagagcagttgagccttctgcttgtccttcattaactaaaggtgtatacagatacgattatggtgacacagcagggatgactccactaatgaagatgcatacacttgggcatgacttcattccggacccaattcatgccggaggattgcgttatcatggaatggctccattgatttctcacgtttatgaactcggtttcatggaagcaatttcaattccccagactgagtgcttcaaaggtgctatacaatttgctaggtccgaaggattgattccagcgccagagccaacccatgccatcgctgccaccattagggaggctctgaagtgtaaagagaccggagaagcaaaagttattctcactgcaatgtgtggacatggccatttcgatttgccagcttatgacaattatctgcaaggaaatctggttgacctgtcattcgaggatgagaagctacaagcagcacttgcattgattgagaagaaatggcttggatctattgcacttgctagaaagccgaagaatttgaagctttcaaatggctgtagagcaagagcagccctcaatgctgattcaataccaatcgaaatccctcgtcagtggtacaacctgattgcagatcttcctataaaacctcctccttcattgcatcccaagacttttgaaccagtaaaacctgaagatctgtcccctctatttcctgatgagttgattaaacaggaagcaagtggtgaaagatacatagatatcccagaagaagttcgagagatatacaagctttggcgtccaacccctctcattagggcaaagaggttggagaagcttcttgacacgcctgccagaatttattacaagtatgaaggtgttagccctgctggatcacacaaacccaacactgcagttcctcaagtgtattataatgcacaacaaggcatcaagaaggttgtgacagaaacaggagctggtcaatggggatgttctttggcttttgcatccagcttattcggtcttgaatgtgaggtatggcaagtgcgtgcttcgtatgatcagaaaccatatcgcaaattgatgatgcaaacttggggggcaaaggtacacccgtctccatcgaacataactgaatcaggtagaaaaatccttctgatggatccatcgaccccaggaagtttaggaatagctatttctgaagctgtggaggttgcagctatgaatgctgacactaaatactgtctgggaagtgttctcaatcatgttttgctccaccagactgtaataggtgaagagtgtctgaaacaaatggaagcgataggtgaaaccccggacttgatcatagggtgtactggtggaggatccaactttgcagggctaagtttcccattcattagagaaaagctcgatgggaaaatgagccctgtaattagagcagttgagccttctgcttgtccttcattaactaaaggtgtatacagatacgattatggtgacacagcagggatgactccactaatgaagatgcatacacttgggcatgacttcattccggacccaattcatgccggaggattgcgttatcatggaatggctccattgatttctcacgtttatgaactcggtttcatggaagcaatttcaattccccagactgagtgcttcaaaggtgctatacaatttgctaggtccgaaggattgattccagcgccagagccaacccatgccatcgctgccaccattagggaggctctgaagtgtaaagagaccggagaagcaaaagttattctcactgcaatgtgtggacatggccatttcgatttgccagcttatgacaattatctgcaaggaaatctggttgacctgtcattcgaggatgagaagctacaagcagcactgggtagtgttcctaacgtagggagctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00400 ko01100 ko01110 ko01130 ko01230 map00260 map00400 map01100 map01110 map01130 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

977

Amino Acids

107.16

Weight (kDa)

6.57

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 127 - 465 7.5e-38 Pyridoxal-phosphate dependent enzyme
PALP PF00291 600 - 938 7.5e-38 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 519, 1938
AccI GTMKAC 2 cut(s) 1065, 2484
AccIII TCCGGA 2 cut(s) 1141, 2560
AcoI YGGCCR 2 cut(s) 1399, 2818
AcsI RAATTY 4 cut(s) 124, 444, 1543, 1863
AcuI CTGAAG 6 cut(s) 300, 807, 1364, 1719, 2226, 2783
AcyI GRCGYC 2 cut(s) 385, 1804
AfaI GTAC 6 cut(s) 206, 696, 940, 1625, 2115, 2359
AfiI CCNNNNNNNGG 5 cut(s) 745, 1332, 2164, 2751, 2924
AjnI CCWGG 1 cut(s) 2179
Alw26I GTCTC 4 cut(s) 708, 1349, 2127, 2768
AlwNI CAGNNNCTG 2 cut(s) 563, 1982
Ama87I CYCGRG 1 cut(s) 760
Aor13HI TCCGGA 2 cut(s) 1141, 2560
AoxI GGCC 2 cut(s) 1399, 2818
ApeKI GCWGC 8 cut(s) 158, 803, 1322, 1481, 1577, 2222, 2741, 2900
ApoI RAATTY 4 cut(s) 124, 444, 1543, 1863
ArsI GACNNNNNNTTYG 4 cut(s) 1442, 1474, 2861, 2893
Asp700I GAANNNNTTC 2 cut(s) 360, 1779
AspLEI GCGC 2 cut(s) 1300, 2719
AspS9I GGNCC 4 cut(s) 1144, 1278, 2563, 2697
AsuC2I CCSGG 4 cut(s) 761, 762, 920, 2339
AsuHPI GGTGA 8 cut(s) 344, 890, 923, 1094, 1763, 2309, 2342, 2513
AvaI CYCGRG 1 cut(s) 760
AvaII GGWCC 4 cut(s) 1144, 1278, 2563, 2697
BaeI ACNNNNGTAYC 4 cut(s) 686, 719, 2105, 2138
BalI TGGCCA 2 cut(s) 1401, 2820
BamHI GGATCC 4 cut(s) 748, 950, 2167, 2369
BanII GRGCYC 2 cut(s) 1013, 2432
BbvI GCAGC 8 cut(s) 170, 815, 1309, 1493, 1589, 2234, 2728, 2912
BciT130I CCWGG 1 cut(s) 2181
BclI TGATCA 4 cut(s) 646, 927, 2065, 2346
BcnI CCSGG 4 cut(s) 761, 762, 920, 2339
BcoDI GTCTC 4 cut(s) 708, 1349, 2127, 2768
BfaI CTAG 3 cut(s) 1275, 1530, 2694
BfmI CTRYAG 4 cut(s) 145, 498, 1564, 1917
BfoI RGCGCY 2 cut(s) 1301, 2720
BglII AGATCT 4 cut(s) 220, 283, 1639, 1702
BisI GCNGC 8 cut(s) 159, 804, 1323, 1482, 1578, 2223, 2742, 2901
BlsI GCNGC 8 cut(s) 160, 805, 1324, 1483, 1579, 2224, 2743, 2902
Bme1390I CCNGG 5 cut(s) 761, 762, 920, 2181, 2339
Bme18I GGWCC 4 cut(s) 1144, 1278, 2563, 2697
BmeT110I CYCGRG 1 cut(s) 760
BmgT120I GGNCC 4 cut(s) 1144, 1278, 2563, 2697
BmiI GGNNCC 8 cut(s) 750, 952, 1146, 1185, 2169, 2371, 2565, 2604
BmrFI CCNGG 5 cut(s) 761, 762, 920, 2181, 2339
BmrI ACTGGG 1 cut(s) 2915
BmuI ACTGGG 1 cut(s) 2915
BplI GAGNNNNNCTC 8 cut(s) 147, 179, 978, 1010, 1566, 1598, 2397, 2429
BpmI CTGGAG 1 cut(s) 55
BpuEI CTTGAG 2 cut(s) 492, 1911
BpuMI CCSGG 4 cut(s) 761, 762, 920, 2339
BsaHI GRCGYC 2 cut(s) 385, 1804
BsaI GGTCTC 2 cut(s) 1349, 2768
BsaJI CCNNGG 5 cut(s) 759, 760, 918, 2179, 2337
BsaWI WCCGGW 4 cut(s) 1141, 1358, 2560, 2777
Bsc4I CCNNNNNNNGG 5 cut(s) 745, 1332, 2164, 2751, 2924
Bse1I ACTGG 5 cut(s) 269, 946, 1688, 2365, 2910
Bse3DI GCAATG 4 cut(s) 245, 1392, 1664, 2811
BseAI TCCGGA 2 cut(s) 1141, 2560
BseBI CCWGG 1 cut(s) 2181
BseDI CCNNGG 5 cut(s) 759, 760, 918, 2179, 2337
BseLI CCNNNNNNNGG 5 cut(s) 745, 1332, 2164, 2751, 2924
BseMI GCAATG 4 cut(s) 245, 1392, 1664, 2811
BseMII CTCAG 2 cut(s) 1236, 2655
BseNI ACTGG 5 cut(s) 269, 946, 1688, 2365, 2910
BseRI GAGGAG 2 cut(s) 228, 1647
BseXI GCAGC 8 cut(s) 170, 815, 1309, 1493, 1589, 2234, 2728, 2912
BshFI GGCC 2 cut(s) 1401, 2820
BsiHKCI CYCGRG 1 cut(s) 760
BsiSI CCGG 9 cut(s) 761, 920, 1142, 1158, 1359, 2339, 2561, 2577, 2778
BslFI GGGAC 2 cut(s) 274, 1693
BslI CCNNNNNNNGG 5 cut(s) 745, 1332, 2164, 2751, 2924
BsmAI GTCTC 4 cut(s) 708, 1349, 2127, 2768
BsmBI CGTCTC 2 cut(s) 708, 2127
BsmFI GGGAC 2 cut(s) 274, 1693
BsmI GAATGC 2 cut(s) 817, 2236
BsnI GGCC 2 cut(s) 1401, 2820
Bso31I GGTCTC 2 cut(s) 1349, 2768
BsoBI CYCGRG 1 cut(s) 760
Bsp1286I GDGCHC 2 cut(s) 1013, 2432
Bsp13I TCCGGA 2 cut(s) 1141, 2560
BspANI GGCC 2 cut(s) 1401, 2820
BspCNI CTCAG 2 cut(s) 1237, 2656
BspEI TCCGGA 2 cut(s) 1141, 2560
BspLI GGNNCC 8 cut(s) 750, 952, 1146, 1185, 2169, 2371, 2565, 2604
BspMAI CTGCAG 2 cut(s) 502, 1921
BspTNI GGTCTC 2 cut(s) 1349, 2768
BsrDI GCAATG 4 cut(s) 245, 1392, 1664, 2811
BsrI ACTGG 5 cut(s) 269, 946, 1688, 2365, 2910
BssECI CCNNGG 5 cut(s) 759, 760, 918, 2179, 2337
BssNAI GTATAC 2 cut(s) 1066, 2485
BssNI GRCGYC 2 cut(s) 385, 1804
Bst1107I GTATAC 2 cut(s) 1066, 2485
Bst2UI CCWGG 1 cut(s) 2181
Bst4CI ACNGT 5 cut(s) 14, 830, 871, 2249, 2290
Bst6I CTCTTC 2 cut(s) 876, 2295
BstACI GRCGYC 2 cut(s) 385, 1804
BstAPI GCANNNNNTGC 1 cut(s) 1487
BstC8I GCNNGC 6 cut(s) 438, 636, 1416, 1857, 2055, 2835
BstDEI CTNAG 5 cut(s) 45, 968, 1245, 2387, 2664
BstENI CCTNNNNNAGG 1 cut(s) 2922
BstH2I RGCGCY 2 cut(s) 1301, 2720
BstHHI GCGC 2 cut(s) 1300, 2719
BstMAI GTCTC 4 cut(s) 708, 1349, 2127, 2768
BstMWI GCNNNNNNNGC 9 cut(s) 158, 167, 812, 1322, 1487, 1577, 1586, 2231, 2741
BstNI CCWGG 1 cut(s) 2181
BstSCI CCNGG 5 cut(s) 759, 760, 918, 2179, 2337
BstSFI CTRYAG 4 cut(s) 145, 498, 1564, 1917
BstV1I GCAGC 8 cut(s) 170, 815, 1309, 1493, 1589, 2234, 2728, 2912
BstZ17I GTATAC 2 cut(s) 1066, 2485
BsuRI GGCC 2 cut(s) 1401, 2820
BtgZI GCGATG 2 cut(s) 1303, 2722
BtsI GCAGTG 4 cut(s) 495, 1380, 1914, 2799
BtsIMutI CAGTG 8 cut(s) 10, 206, 495, 1380, 1625, 1914, 2799, 2903
Cac8I GCNNGC 6 cut(s) 438, 636, 1416, 1857, 2055, 2835
CaiI CAGNNNCTG 2 cut(s) 563, 1982
CfoI GCGC 2 cut(s) 1300, 2719
Cfr13I GGNCC 4 cut(s) 1144, 1278, 2563, 2697
Cfr9I CCCGGG 1 cut(s) 760
CseI GACGC 2 cut(s) 374, 1793
Csp6I GTAC 6 cut(s) 205, 695, 939, 1624, 2114, 2358
CviQI GTAC 6 cut(s) 205, 695, 939, 1624, 2114, 2358
DdeI CTNAG 5 cut(s) 45, 968, 1245, 2387, 2664
EaeI YGGCCR 2 cut(s) 1399, 2818
Eam1104I CTCTTC 2 cut(s) 876, 2295
EarI CTCTTC 2 cut(s) 876, 2295
Eco24I GRGCYC 2 cut(s) 1013, 2432
Eco31I GGTCTC 2 cut(s) 1349, 2768
Eco32I GATATC 2 cut(s) 349, 1768
Eco47I GGWCC 4 cut(s) 1144, 1278, 2563, 2697
Eco57I CTGAAG 6 cut(s) 300, 807, 1364, 1719, 2226, 2783
Eco88I CYCGRG 1 cut(s) 760
EcoNI CCTNNNNNAGG 1 cut(s) 2922
EcoRII CCWGG 1 cut(s) 2179
EcoRV GATATC 2 cut(s) 349, 1768
EcoT22I ATGCAT 2 cut(s) 1119, 2538
EcoT38I GRGCYC 2 cut(s) 1013, 2432
Esp3I CGTCTC 2 cut(s) 708, 2127
FalI AAGNNNNNCTT 2 cut(s) 1470, 1502
FaqI GGGAC 2 cut(s) 274, 1693
FbaI TGATCA 4 cut(s) 646, 927, 2065, 2346
FblI GTMKAC 2 cut(s) 1065, 2484
Fnu4HI GCNGC 8 cut(s) 159, 804, 1323, 1482, 1578, 2223, 2742, 2901
FriOI GRGCYC 2 cut(s) 1013, 2432
Fsp4HI GCNGC 8 cut(s) 159, 804, 1323, 1482, 1578, 2223, 2742, 2901
FspBI CTAG 3 cut(s) 1275, 1530, 2694
GlaI GCGC 2 cut(s) 1299, 2718
GluI GCNGC 8 cut(s) 159, 804, 1323, 1482, 1578, 2223, 2742, 2901
GsuI CTGGAG 1 cut(s) 55
HaeII RGCGCY 2 cut(s) 1301, 2720
HaeIII GGCC 2 cut(s) 1401, 2820
HapII CCGG 9 cut(s) 761, 920, 1142, 1158, 1359, 2339, 2561, 2577, 2778
HgaI GACGC 2 cut(s) 374, 1793
HhaI GCGC 2 cut(s) 1300, 2719
Hin1I GRCGYC 2 cut(s) 385, 1804
Hin6I GCGC 2 cut(s) 1298, 2717
HinP1I GCGC 2 cut(s) 1298, 2717
HincII GTYRAC 2 cut(s) 1450, 2869
HindII GTYRAC 2 cut(s) 1450, 2869
HindIII AAGCTT 6 cut(s) 131, 377, 422, 1550, 1796, 1841
HinfI GANTC 8 cut(s) 173, 722, 1099, 1291, 1592, 2141, 2518, 2710
HpaII CCGG 9 cut(s) 761, 920, 1142, 1158, 1359, 2339, 2561, 2577, 2778
HphI GGTGA 8 cut(s) 344, 890, 923, 1094, 1763, 2309, 2342, 2513
HpyCH4III ACNGT 5 cut(s) 14, 830, 871, 2249, 2290
HpyCH4IV ACGT 3 cut(s) 1200, 2619, 2922
HpyF10VI GCNNNNNNNGC 9 cut(s) 158, 167, 812, 1322, 1487, 1577, 1586, 2231, 2741
HpyF3I CTNAG 5 cut(s) 45, 968, 1245, 2387, 2664
HpySE526I ACGT 3 cut(s) 1200, 2619, 2922
Hsp92I GRCGYC 2 cut(s) 385, 1804
HspAI GCGC 2 cut(s) 1298, 2717
Kpn2I TCCGGA 2 cut(s) 1141, 2560
Ksp22I TGATCA 4 cut(s) 646, 927, 2065, 2346
LmnI GCTCC 7 cut(s) 560, 864, 1189, 1979, 2283, 2608, 2928
Lsp1109I GCAGC 8 cut(s) 170, 815, 1309, 1493, 1589, 2234, 2728, 2912
MaeI CTAG 3 cut(s) 1275, 1530, 2694
MaeII ACGT 3 cut(s) 1200, 2619, 2922
MaeIII GTNAC 4 cut(s) 547, 1082, 1966, 2501
MhlI GDGCHC 2 cut(s) 1013, 2432
MlsI TGGCCA 2 cut(s) 1401, 2820
MluNI TGGCCA 2 cut(s) 1401, 2820
MlyI GAGTC 2 cut(s) 1093, 2512
MmeI TCCRAC 6 cut(s) 396, 413, 978, 1815, 1832, 2397
Mox20I TGGCCA 2 cut(s) 1401, 2820
Mph1103I ATGCAT 2 cut(s) 1119, 2538
MroI TCCGGA 2 cut(s) 1141, 2560
MroXI GAANNNNTTC 2 cut(s) 360, 1779
MscI TGGCCA 2 cut(s) 1401, 2820
MseI TTAA 5 cut(s) 18, 315, 1052, 1734, 2471
MslI CAYNNNNRTG 4 cut(s) 1203, 1385, 2622, 2804
Msp20I TGGCCA 2 cut(s) 1401, 2820
MspI CCGG 9 cut(s) 761, 920, 1142, 1158, 1359, 2339, 2561, 2577, 2778
MspR9I CCNGG 5 cut(s) 761, 762, 920, 2181, 2339
Mva1269I GAATGC 2 cut(s) 817, 2236
MvaI CCWGG 1 cut(s) 2181
MwoI GCNNNNNNNGC 9 cut(s) 158, 167, 812, 1322, 1487, 1577, 1586, 2231, 2741
NciI CCSGG 4 cut(s) 761, 762, 920, 2339
NlaIV GGNNCC 8 cut(s) 750, 952, 1146, 1185, 2169, 2371, 2565, 2604
NmuCI GTSAC 4 cut(s) 547, 1082, 1966, 2501
NsiI ATGCAT 2 cut(s) 1119, 2538
PctI GAATGC 2 cut(s) 817, 2236
PdmI GAANNNNTTC 2 cut(s) 360, 1779
PfeI GAWTC 6 cut(s) 173, 722, 1291, 1592, 2141, 2710
PkrI GCNGC 8 cut(s) 160, 805, 1324, 1483, 1579, 2224, 2743, 2902
PleI GAGTC 2 cut(s) 1093, 2512
PpsI GAGTC 2 cut(s) 1093, 2512
PsiI TTATAA 2 cut(s) 519, 1938
Psp6I CCWGG 1 cut(s) 2179
PspGI CCWGG 1 cut(s) 2179
PspN4I GGNNCC 8 cut(s) 750, 952, 1146, 1185, 2169, 2371, 2565, 2604
PspPI GGNCC 4 cut(s) 1144, 1278, 2563, 2697
PstI CTGCAG 2 cut(s) 502, 1921
PstNI CAGNNNCTG 2 cut(s) 563, 1982
RsaI GTAC 6 cut(s) 206, 696, 940, 1625, 2115, 2359
RsaNI GTAC 6 cut(s) 205, 695, 939, 1624, 2114, 2358
RseI CAYNNNNRTG 4 cut(s) 1203, 1385, 2622, 2804
SaqAI TTAA 5 cut(s) 18, 315, 1052, 1734, 2471
SatI GCNGC 8 cut(s) 159, 804, 1323, 1482, 1578, 2223, 2742, 2901
Sau96I GGNCC 4 cut(s) 1144, 1278, 2563, 2697
SchI GAGTC 2 cut(s) 1093, 2512
ScrFI CCNGG 5 cut(s) 761, 762, 920, 2181, 2339
SduI GDGCHC 2 cut(s) 1013, 2432
SfcI CTRYAG 4 cut(s) 145, 498, 1564, 1917
SinI GGWCC 4 cut(s) 1144, 1278, 2563, 2697
SmaI CCCGGG 1 cut(s) 762
SmiMI CAYNNNNRTG 4 cut(s) 1203, 1385, 2622, 2804
SmlI CTYRAG 2 cut(s) 507, 1926
SmoI CTYRAG 2 cut(s) 507, 1926
SspMI CTAG 3 cut(s) 1275, 1530, 2694
StyD4I CCNGG 5 cut(s) 759, 760, 918, 2179, 2337
TaaI ACNGT 5 cut(s) 14, 830, 871, 2249, 2290
TaiI ACGT 3 cut(s) 1203, 2622, 2925
TaqII GACCGA 2 cut(s) 596, 2015
TatI WGTACW 2 cut(s) 938, 2357
TfiI GAWTC 6 cut(s) 173, 722, 1291, 1592, 2141, 2710
Tru1I TTAA 5 cut(s) 18, 315, 1052, 1734, 2471
Tru9I TTAA 5 cut(s) 18, 315, 1052, 1734, 2471
TscAI CASTG 8 cut(s) 17, 206, 502, 1387, 1625, 1921, 2806, 2910
TseFI GTSAC 4 cut(s) 547, 1082, 1966, 2501
TseI GCWGC 8 cut(s) 158, 803, 1322, 1481, 1577, 2222, 2741, 2900
Tsp45I GTSAC 4 cut(s) 547, 1082, 1966, 2501
TspMI CCCGGG 1 cut(s) 760
TspRI CASTG 8 cut(s) 17, 206, 502, 1387, 1625, 1921, 2806, 2910
VpaK11BI GGWCC 4 cut(s) 1144, 1278, 2563, 2697
XagI CCTNNNNNAGG 1 cut(s) 2922
XapI RAATTY 4 cut(s) 124, 444, 1543, 1863
XmaI CCCGGG 1 cut(s) 760
XmiI GTMKAC 2 cut(s) 1065, 2484
XmnI GAANNNNTTC 2 cut(s) 360, 1779
XspI CTAG 3 cut(s) 1275, 1530, 2694
Zsp2I ATGCAT 2 cut(s) 1119, 2538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.