Rmu_sc0001193.1_g000005

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001193.1
N/A
Physical Location & Seq
Reverse (-)
23830 .. 27319
3490 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 660 bp
atgtcgggtcggggcaagggcggtaagggtttgggcaaaggcggagccaagcgccacaggaaggtcctccgcgacaacatccagggcatcaccaagccggccatccgccgtctggctcgccgtggcggcgtcaagcgtatcagtggcctcatctacgaggagacacgtggcgtcctcaagatctttctggagaacgtcatccgcgacgccgtgacgtacaccgagcacgccaggaggaagacggtgacggccatggatgtggtgtacgcgctcaagaggcagggcaggactctctacgggttcggcggtcgggtcggaattgacagccctatgtctagggttagtcacaagattttggttgtgatccatgaccttcaaatcccaggaaccaccgccgcccaccttgaagctttcacctctaggagtccagaaccgaaggcgctgcccccaaaactggccggaattaacctgaaccgatactgcaccaccaaaccgctgcatactgcagttaacccagcccagcaacccaaagcttcggcccaaaaggaaagaaaaaccctatgcccagaagcacagctggatctcggcccaaagtccaaagcccagaagcccaacagccagaagcccactgacgtcagccctgcgtcatcagccacgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
CENP-T_C

Protein Analysis

219

Amino Acids

23.8

Weight (kDa)

10.74

Isoelectric Point (pI)

50.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CENP-T_C PF15511 44 - 96 1.7e-07 Centromere kinetochore component CENP-T histone fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 636
AccII CGCG 3 cut(s) 72, 204, 269
AclWI GGATC 2 cut(s) 358, 588
AcoI YGGCCR 3 cut(s) 99, 249, 456
AcvI CACGTG 1 cut(s) 167
AcyI GRCGYC 4 cut(s) 129, 171, 207, 633
AfaI GTAC 2 cut(s) 218, 266
AfiI CCNNNNNNNGG 3 cut(s) 61, 112, 454
AflIII ACRYGT 1 cut(s) 164
AgsI TTSAA 2 cut(s) 377, 407
AjnI CCWGG 3 cut(s) 81, 230, 382
AluBI AGCT 3 cut(s) 410, 533, 577
AluI AGCT 3 cut(s) 410, 533, 577
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 1 cut(s) 155
AlwI GGATC 2 cut(s) 358, 588
AoxI GGCC 6 cut(s) 99, 145, 249, 456, 537, 586
ApeKI GCWGC 2 cut(s) 442, 496
AspLEI GCGC 3 cut(s) 54, 271, 442
AspS9I GGNCC 3 cut(s) 64, 538, 587
AsuHPI GGTGA 3 cut(s) 82, 256, 406
AvaII GGWCC 1 cut(s) 64
BbrPI CACGTG 1 cut(s) 167
BbsI GAAGAC 1 cut(s) 245
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 2 cut(s) 429, 483
BccI CCATC 1 cut(s) 110
BceAI ACGGC 4 cut(s) 93, 105, 194, 264
BcgI CGANNNNNNTGC 2 cut(s) 424, 458
BciT130I CCWGG 3 cut(s) 83, 232, 384
BcoDI GTCTC 1 cut(s) 155
BfaI CTAG 2 cut(s) 336, 420
BfmI CTRYAG 1 cut(s) 504
BfoI RGCGCY 2 cut(s) 55, 443
BglI GCCNNNNNGGC 1 cut(s) 126
BglII AGATCT 1 cut(s) 180
BisI GCNGC 4 cut(s) 127, 396, 443, 497
BlsI GCNGC 4 cut(s) 128, 397, 444, 498
Bme1390I CCNGG 3 cut(s) 83, 232, 384
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 3 cut(s) 64, 538, 587
BmiI GGNNCC 2 cut(s) 46, 388
BmrFI CCNGG 3 cut(s) 83, 232, 384
BmsI GCATC 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 245
BpmI CTGGAG 1 cut(s) 209
BpuEI CTTGAG 2 cut(s) 161, 257
BsaAI YACGTR 2 cut(s) 167, 657
BsaHI GRCGYC 4 cut(s) 129, 171, 207, 633
BsaJI CCNNGG 4 cut(s) 82, 121, 252, 382
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 112, 454
Bse118I RCCGGY 1 cut(s) 97
Bse1I ACTGG 1 cut(s) 459
BseBI CCWGG 3 cut(s) 83, 232, 384
BseDI CCNNGG 4 cut(s) 82, 121, 252, 382
BseGI GGATG 4 cut(s) 78, 102, 198, 262
BseLI CCNNNNNNNGG 3 cut(s) 61, 112, 454
BseNI ACTGG 1 cut(s) 459
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 2 cut(s) 429, 483
BseYI CCCAGC 2 cut(s) 514, 519
BsgI GTGCAG 1 cut(s) 466
Bsh1236I CGCG 3 cut(s) 72, 204, 269
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 6 cut(s) 101, 147, 251, 458, 539, 588
BsiEI CGRYCG 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 228
BsiSI CCGG 2 cut(s) 98, 459
BslI CCNNNNNNNGG 3 cut(s) 61, 112, 454
BsmAI GTCTC 1 cut(s) 155
BsnI GGCC 6 cut(s) 101, 147, 251, 458, 539, 588
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 3 cut(s) 180, 363, 580
Bsp19I CCATGG 1 cut(s) 252
BspANI GGCC 6 cut(s) 101, 147, 251, 458, 539, 588
BspFNI CGCG 3 cut(s) 72, 204, 269
BspLI GGNNCC 2 cut(s) 46, 388
BspMAI CTGCAG 1 cut(s) 508
BspPI GGATC 2 cut(s) 358, 588
BsrFI RCCGGY 1 cut(s) 97
BsrI ACTGG 1 cut(s) 459
BssAI RCCGGY 1 cut(s) 97
BssECI CCNNGG 4 cut(s) 82, 121, 252, 382
BssMI GATC 3 cut(s) 180, 363, 580
BssNI GRCGYC 4 cut(s) 129, 171, 207, 633
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 3 cut(s) 83, 232, 384
Bst4CI ACNGT 1 cut(s) 244
BstACI GRCGYC 4 cut(s) 129, 171, 207, 633
BstBAI YACGTR 2 cut(s) 167, 657
BstC8I GCNNGC 3 cut(s) 99, 118, 228
BstDSI CCRYGG 2 cut(s) 121, 252
BstF5I GGATG 4 cut(s) 78, 102, 198, 262
BstFNI CGCG 3 cut(s) 72, 204, 269
BstH2I RGCGCY 2 cut(s) 55, 443
BstHHI GCGC 3 cut(s) 54, 271, 442
BstKTI GATC 3 cut(s) 183, 366, 583
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 3 cut(s) 180, 363, 580
BstMCI CGRYCG 1 cut(s) 310
BstMWI GCNNNNNNNGC 3 cut(s) 126, 277, 650
BstNI CCWGG 3 cut(s) 83, 232, 384
BstSCI CCNGG 3 cut(s) 81, 230, 382
BstSFI CTRYAG 1 cut(s) 504
BstUI CGCG 3 cut(s) 72, 204, 269
BstV1I GCAGC 2 cut(s) 429, 483
BstV2I GAAGAC 1 cut(s) 245
BstX2I RGATCY 2 cut(s) 180, 580
BstXI CCANNNNNNTGG 1 cut(s) 259
BstYI RGATCY 2 cut(s) 180, 580
BsuRI GGCC 6 cut(s) 101, 147, 251, 458, 539, 588
BtgI CCRYGG 2 cut(s) 121, 252
BtsCI GGATG 4 cut(s) 78, 102, 198, 262
BtsIMutI CAGTG 2 cut(s) 148, 627
Cac8I GCNNGC 3 cut(s) 99, 118, 228
CfoI GCGC 3 cut(s) 54, 271, 442
Cfr10I RCCGGY 1 cut(s) 97
Cfr13I GGNCC 3 cut(s) 64, 538, 587
CseI GACGC 4 cut(s) 118, 160, 215, 633
Csp6I GTAC 2 cut(s) 217, 265
CviAII CATG 2 cut(s) 253, 368
CviQI GTAC 2 cut(s) 217, 265
DpnI GATC 3 cut(s) 182, 365, 582
DpnII GATC 3 cut(s) 180, 363, 580
EaeI YGGCCR 3 cut(s) 99, 249, 456
EciI GGCGGA 2 cut(s) 57, 95
Eco130I CCWWGG 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 64
Eco72I CACGTG 1 cut(s) 167
EcoO109I RGGNCCY 1 cut(s) 64
EcoRII CCWGG 3 cut(s) 81, 230, 382
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 2 cut(s) 256, 371
FaiI YATR 5 cut(s) 254, 332, 369, 501, 562
FatI CATG 2 cut(s) 252, 367
Fnu4HI GCNGC 4 cut(s) 127, 396, 443, 497
FokI GGATG 4 cut(s) 65, 89, 185, 269
Fsp4HI GCNGC 4 cut(s) 127, 396, 443, 497
FspBI CTAG 2 cut(s) 336, 420
GlaI GCGC 3 cut(s) 53, 270, 441
GluI GCNGC 4 cut(s) 127, 396, 443, 497
GsaI CCCAGC 2 cut(s) 518, 523
GsuI CTGGAG 1 cut(s) 209
HaeII RGCGCY 2 cut(s) 55, 443
HaeIII GGCC 6 cut(s) 101, 147, 251, 458, 539, 588
HapII CCGG 2 cut(s) 98, 459
HgaI GACGC 4 cut(s) 118, 160, 215, 633
HhaI GCGC 3 cut(s) 54, 271, 442
Hin1I GRCGYC 4 cut(s) 129, 171, 207, 633
Hin1II CATG 2 cut(s) 256, 371
Hin6I GCGC 3 cut(s) 52, 269, 440
HinP1I GCGC 3 cut(s) 52, 269, 440
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 2 cut(s) 408, 531
HinfI GANTC 2 cut(s) 289, 424
HpaI GTTAAC 1 cut(s) 511
HpaII CCGG 2 cut(s) 98, 459
HphI GGTGA 3 cut(s) 82, 256, 406
Hpy166II GTNNAC 3 cut(s) 219, 265, 511
Hpy188I TCNGA 1 cut(s) 317
Hpy188III TCNNGA 4 cut(s) 178, 188, 274, 428
Hpy8I GTNNAC 3 cut(s) 219, 265, 511
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 3 cut(s) 55, 383, 430
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4IV ACGT 5 cut(s) 166, 195, 215, 633, 656
HpyCH4V TGCA 3 cut(s) 483, 499, 506
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 277, 650
HpySE526I ACGT 5 cut(s) 166, 195, 215, 633, 656
Hsp92I GRCGYC 4 cut(s) 129, 171, 207, 633
Hsp92II CATG 2 cut(s) 256, 371
HspAI GCGC 3 cut(s) 52, 269, 440
KroI GCCGGC 1 cut(s) 97
KroNI GCCGGC 1 cut(s) 99
KspAI GTTAAC 1 cut(s) 511
Kzo9I GATC 3 cut(s) 180, 363, 580
LmnI GCTCC 1 cut(s) 44
Lsp1109I GCAGC 2 cut(s) 429, 483
LweI GCATC 1 cut(s) 96
MaeI CTAG 2 cut(s) 336, 420
MaeII ACGT 5 cut(s) 166, 195, 215, 633, 656
MaeIII GTNAC 3 cut(s) 211, 244, 344
MalI GATC 3 cut(s) 182, 365, 582
MboI GATC 3 cut(s) 180, 363, 580
MboII GAAGA 1 cut(s) 250
MflI RGATCY 2 cut(s) 180, 580
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 2 cut(s) 318, 462
MlyI GAGTC 2 cut(s) 283, 433
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 7 cut(s) 77, 151, 158, 185, 228, 270, 427
MroNI GCCGGC 1 cut(s) 97
MseI TTAA 2 cut(s) 465, 510
MslI CAYNNNNRTG 1 cut(s) 257
MspA1I CMGCKG 2 cut(s) 496, 577
MspI CCGG 2 cut(s) 98, 459
MspR9I CCNGG 3 cut(s) 83, 232, 384
MvaI CCWGG 3 cut(s) 83, 232, 384
MvnI CGCG 3 cut(s) 72, 204, 269
MwoI GCNNNNNNNGC 3 cut(s) 126, 277, 650
NaeI GCCGGC 1 cut(s) 99
NcoI CCATGG 1 cut(s) 252
NdeII GATC 3 cut(s) 180, 363, 580
NgoMIV GCCGGC 1 cut(s) 97
NlaIII CATG 2 cut(s) 256, 371
NlaIV GGNNCC 2 cut(s) 46, 388
NmeAIII GCCGAG 1 cut(s) 564
NmuCI GTSAC 3 cut(s) 211, 244, 344
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PdiI GCCGGC 1 cut(s) 99
PkrI GCNGC 4 cut(s) 128, 397, 444, 498
PleI GAGTC 2 cut(s) 283, 432
PmaCI CACGTG 1 cut(s) 167
PmlI CACGTG 1 cut(s) 167
PpsI GAGTC 2 cut(s) 283, 432
Ppu21I YACGTR 2 cut(s) 167, 657
PpuMI RGGWCCY 1 cut(s) 64
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 3 cut(s) 81, 230, 382
PspCI CACGTG 1 cut(s) 167
PspFI CCCAGC 2 cut(s) 514, 519
PspGI CCWGG 3 cut(s) 81, 230, 382
PspN4I GGNNCC 2 cut(s) 46, 388
PspPI GGNCC 3 cut(s) 64, 538, 587
PspPPI RGGWCCY 1 cut(s) 64
PstI CTGCAG 1 cut(s) 508
PsuI RGATCY 2 cut(s) 180, 580
PvuII CAGCTG 1 cut(s) 577
RsaI GTAC 2 cut(s) 218, 266
RsaNI GTAC 2 cut(s) 217, 265
RseI CAYNNNNRTG 1 cut(s) 257
SaqAI TTAA 2 cut(s) 465, 510
SatI GCNGC 4 cut(s) 127, 396, 443, 497
Sau3AI GATC 3 cut(s) 180, 363, 580
Sau96I GGNCC 3 cut(s) 64, 538, 587
SchI GAGTC 2 cut(s) 283, 433
ScrFI CCNGG 3 cut(s) 83, 232, 384
SduI GDGCHC 1 cut(s) 228
SfaNI GCATC 1 cut(s) 96
SfcI CTRYAG 1 cut(s) 504
SinI GGWCC 1 cut(s) 64
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 2 cut(s) 176, 272
SmoI CTYRAG 2 cut(s) 176, 272
Sse9I AATT 2 cut(s) 318, 462
SspMI CTAG 2 cut(s) 336, 420
StyD4I CCNGG 3 cut(s) 81, 230, 382
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 244
TaiI ACGT 5 cut(s) 169, 198, 218, 636, 659
TasI AATT 2 cut(s) 318, 462
TauI GCSGC 2 cut(s) 129, 398
Tru1I TTAA 2 cut(s) 465, 510
Tru9I TTAA 2 cut(s) 465, 510
TscAI CASTG 2 cut(s) 148, 634
TseFI GTSAC 3 cut(s) 211, 244, 344
TseI GCWGC 2 cut(s) 442, 496
Tsp45I GTSAC 3 cut(s) 211, 244, 344
TspRI CASTG 2 cut(s) 148, 634
VpaK11BI GGWCC 1 cut(s) 64
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XspI CTAG 2 cut(s) 336, 420
ZraI GACGTC 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.