Rmu_sc0001228.1_g000002

Belongs to the glycosyl hydrolase 13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001228.1
N/A
Physical Location & Seq
Forward (+)
19708 .. 21710
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 501 bp
ctgaccaagtcgctgcaaagtgtcgattgcagttcatatagacaccaaagtcgtcgtcgctgcgcaaaggagaatgcaaaccatctcgcccgatcgaaccaccagaaacgcctagcaccgtcttcgagccagaatcgctactgcccaccaccgcagtatgtctccgagccagcattgaccaagtcagatccaaatcacaccattcaaaacgcggctccgcgaacatggccaaaccacctgcgcgttcctccatctgagagcatagaagatattgttatagaccacagatgtgcagagaagaaagatgagagaggaatattgtgcatttttgaaggaggaacacccgatgatcgccttgattggggcccgtctcttgtttgcagcgacgacacagagttttctgatggcaaggggaaccctgatactggaaatagatggggtggggtacctgacattgatcacaaaaacacaaggtccagaatgagctctcggattggatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

166

Amino Acids

18.63

Weight (kDa)

8.28

Isoelectric Point (pI)

63.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 246
AasI GACNNNNNNGTC 1 cut(s) 48
Acc16I TGCGCA 1 cut(s) 64
Acc36I ACCTGC 1 cut(s) 246
Acc65I GGTACC 1 cut(s) 445
AccB1I GGYRCC 1 cut(s) 445
AccII CGCG 3 cut(s) 212, 220, 243
AciI CCGC 3 cut(s) 152, 212, 218
AclWI GGATC 1 cut(s) 182
AcoI YGGCCR 1 cut(s) 227
AfaI GTAC 1 cut(s) 447
AfiI CCNNNNNNNGG 2 cut(s) 361, 425
AgsI TTSAA 2 cut(s) 206, 332
AleI CACNNNNGTG 1 cut(s) 288
AluBI AGCT 1 cut(s) 486
AluI AGCT 1 cut(s) 486
Alw21I GWGCWC 1 cut(s) 488
Alw26I GTCTC 2 cut(s) 166, 375
AlwI GGATC 1 cut(s) 182
AoxI GGCC 2 cut(s) 227, 364
ApaI GGGCCC 1 cut(s) 368
ApeKI GCWGC 3 cut(s) 13, 60, 381
ArsI GACNNNNNNTTYG 2 cut(s) 40, 72
Asp718I GGTACC 1 cut(s) 445
AspLEI GCGC 2 cut(s) 65, 243
AspS9I GGNCC 3 cut(s) 364, 365, 474
AvaII GGWCC 1 cut(s) 474
BaeGI GKGCMC 1 cut(s) 368
BalI TGGCCA 1 cut(s) 229
BanI GGYRCC 1 cut(s) 445
BanII GRGCYC 2 cut(s) 368, 488
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 488
BbvI GCAGC 2 cut(s) 47, 393
BccI CCATC 4 cut(s) 90, 259, 398, 429
BcgI CGANNNNNNTGC 2 cut(s) 105, 139
BclI TGATCA 1 cut(s) 457
BcoDI GTCTC 2 cut(s) 166, 375
BfaI CTAG 1 cut(s) 113
BfuAI ACCTGC 1 cut(s) 246
BisI GCNGC 4 cut(s) 14, 61, 213, 382
BlsI GCNGC 4 cut(s) 15, 62, 214, 383
Bme18I GGWCC 1 cut(s) 474
BmgT120I GGNCC 3 cut(s) 364, 365, 474
BmiI GGNNCC 5 cut(s) 216, 365, 366, 416, 447
BpiI GAAGAC 1 cut(s) 114
BsaBI GATNNNNATC 1 cut(s) 192
Bsc4I CCNNNNNNNGG 2 cut(s) 361, 425
Bse1I ACTGG 1 cut(s) 430
Bse8I GATNNNNATC 1 cut(s) 192
BseJI GATNNNNATC 1 cut(s) 192
BseLI CCNNNNNNNGG 2 cut(s) 361, 425
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 430
BseSI GKGCMC 1 cut(s) 368
BseXI GCAGC 2 cut(s) 47, 393
BsgI GTGCAG 1 cut(s) 312
Bsh1236I CGCG 3 cut(s) 212, 220, 243
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 2 cut(s) 229, 366
BshNI GGYRCC 1 cut(s) 445
BsiEI CGRYCG 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 488
BslI CCNNNNNNNGG 2 cut(s) 361, 425
BsmAI GTCTC 2 cut(s) 166, 375
BsmBI CGTCTC 1 cut(s) 375
BsmI GAATGC 1 cut(s) 79
BsnI GGCC 2 cut(s) 229, 366
Bsp120I GGGCCC 1 cut(s) 364
Bsp1286I GDGCHC 2 cut(s) 368, 488
Bsp143I GATC 4 cut(s) 92, 187, 349, 457
BspACI CCGC 3 cut(s) 152, 212, 218
BspANI GGCC 2 cut(s) 229, 366
BspCNI CTCAG 1 cut(s) 247
BspFNI CGCG 3 cut(s) 212, 220, 243
BspLI GGNNCC 5 cut(s) 216, 365, 366, 416, 447
BspMI ACCTGC 1 cut(s) 246
BspPI GGATC 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 445
BsrI ACTGG 1 cut(s) 430
BssMI GATC 4 cut(s) 92, 187, 349, 457
Bst4CI ACNGT 1 cut(s) 120
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 1 cut(s) 255
BstFNI CGCG 3 cut(s) 212, 220, 243
BstHHI GCGC 2 cut(s) 65, 243
BstKTI GATC 4 cut(s) 95, 190, 352, 460
BstMAI GTCTC 2 cut(s) 166, 375
BstMBI GATC 4 cut(s) 92, 187, 349, 457
BstMCI CGRYCG 1 cut(s) 95
BstMWI GCNNNNNNNGC 2 cut(s) 135, 226
BstSLI GKGCMC 1 cut(s) 368
BstUI CGCG 3 cut(s) 212, 220, 243
BstV1I GCAGC 2 cut(s) 47, 393
BstV2I GAAGAC 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 187
BstYI RGATCY 1 cut(s) 187
BsuRI GGCC 2 cut(s) 229, 366
BveI ACCTGC 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 171
CfoI GCGC 2 cut(s) 65, 243
Cfr13I GGNCC 3 cut(s) 364, 365, 474
Csp6I GTAC 1 cut(s) 446
CviAII CATG 1 cut(s) 225
CviJI RGCY 6 cut(s) 129, 169, 215, 229, 366, 486
CviKI_1 RGCY 6 cut(s) 129, 169, 215, 229, 366, 486
CviQI GTAC 1 cut(s) 446
DdeI CTNAG 1 cut(s) 255
DpnI GATC 4 cut(s) 94, 189, 351, 459
DpnII GATC 4 cut(s) 92, 187, 349, 457
DrdI GACNNNNNNGTC 1 cut(s) 48
DseDI GACNNNNNNGTC 1 cut(s) 48
EaeI YGGCCR 1 cut(s) 227
Ecl136II GAGCTC 1 cut(s) 486
Eco24I GRGCYC 2 cut(s) 368, 488
Eco47I GGWCC 1 cut(s) 474
Eco53kI GAGCTC 1 cut(s) 486
EcoICRI GAGCTC 1 cut(s) 486
EcoO109I RGGNCCY 1 cut(s) 364
EcoT38I GRGCYC 2 cut(s) 368, 488
Esp3I CGTCTC 1 cut(s) 375
FaeI CATG 1 cut(s) 228
FaiI YATR 6 cut(s) 37, 39, 159, 226, 263, 278
FatI CATG 1 cut(s) 224
FbaI TGATCA 1 cut(s) 457
Fnu4HI GCNGC 4 cut(s) 14, 61, 213, 382
FriOI GRGCYC 2 cut(s) 368, 488
Fsp4HI GCNGC 4 cut(s) 14, 61, 213, 382
FspBI CTAG 1 cut(s) 113
FspI TGCGCA 1 cut(s) 64
GlaI GCGC 2 cut(s) 64, 242
GluI GCNGC 4 cut(s) 14, 61, 213, 382
HaeIII GGCC 2 cut(s) 229, 366
HhaI GCGC 2 cut(s) 65, 243
Hin1II CATG 1 cut(s) 228
Hin6I GCGC 2 cut(s) 63, 241
HinP1I GCGC 2 cut(s) 63, 241
HinfI GANTC 1 cut(s) 133
Hpy188I TCNGA 5 cut(s) 166, 187, 256, 403, 492
Hpy188III TCNNGA 1 cut(s) 477
Hpy99I CGWCG 3 cut(s) 57, 60, 389
HpyAV CCTTC 1 cut(s) 326
HpyCH4III ACNGT 1 cut(s) 120
HpyCH4V TGCA 6 cut(s) 16, 30, 77, 293, 324, 381
HpyF10VI GCNNNNNNNGC 2 cut(s) 135, 226
HpyF3I CTNAG 1 cut(s) 255
Hsp92II CATG 1 cut(s) 228
HspAI GCGC 2 cut(s) 63, 241
KpnI GGTACC 1 cut(s) 449
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 4 cut(s) 92, 187, 349, 457
LmnI GCTCC 1 cut(s) 220
LpnPI CCDG 8 cut(s) 116, 143, 183, 251, 411, 432, 462, 490
Lsp1109I GCAGC 2 cut(s) 47, 393
MaeI CTAG 1 cut(s) 113
MalI GATC 4 cut(s) 94, 189, 351, 459
MboI GATC 4 cut(s) 92, 187, 349, 457
MboII GAAGA 3 cut(s) 114, 278, 310
MflI RGATCY 1 cut(s) 187
MhlI GDGCHC 2 cut(s) 368, 488
MlsI TGGCCA 1 cut(s) 229
MluNI TGGCCA 1 cut(s) 229
MnlI CCTC 3 cut(s) 258, 305, 329
Mox20I TGGCCA 1 cut(s) 229
MscI TGGCCA 1 cut(s) 229
MslI CAYNNNNRTG 1 cut(s) 288
Msp20I TGGCCA 1 cut(s) 229
Mva1269I GAATGC 1 cut(s) 79
MvnI CGCG 3 cut(s) 212, 220, 243
MwoI GCNNNNNNNGC 2 cut(s) 135, 226
NdeII GATC 4 cut(s) 92, 187, 349, 457
NlaIII CATG 1 cut(s) 228
NlaIV GGNNCC 5 cut(s) 216, 365, 366, 416, 447
NsbI TGCGCA 1 cut(s) 64
OliI CACNNNNGTG 1 cut(s) 288
PaqCI CACCTGC 1 cut(s) 246
PctI GAATGC 1 cut(s) 79
PfeI GAWTC 1 cut(s) 133
PflFI GACNNNGTC 2 cut(s) 7, 181
PkrI GCNGC 4 cut(s) 15, 62, 214, 383
Ple19I CGATCG 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 488
PspN4I GGNNCC 5 cut(s) 216, 365, 366, 416, 447
PspOMI GGGCCC 1 cut(s) 364
PspPI GGNCC 3 cut(s) 364, 365, 474
PsuI RGATCY 1 cut(s) 187
PsyI GACNNNGTC 2 cut(s) 7, 181
PvuI CGATCG 1 cut(s) 95
RsaI GTAC 1 cut(s) 447
RsaNI GTAC 1 cut(s) 446
RseI CAYNNNNRTG 1 cut(s) 288
SacI GAGCTC 1 cut(s) 488
SatI GCNGC 4 cut(s) 14, 61, 213, 382
Sau3AI GATC 4 cut(s) 92, 187, 349, 457
Sau96I GGNCC 3 cut(s) 364, 365, 474
SduI GDGCHC 2 cut(s) 368, 488
SetI ASST 4 cut(s) 240, 451, 476, 488
SinI GGWCC 1 cut(s) 474
SmiMI CAYNNNNRTG 1 cut(s) 288
SsiI CCGC 3 cut(s) 152, 212, 218
SspI AATATT 1 cut(s) 318
SspMI CTAG 1 cut(s) 113
SstI GAGCTC 1 cut(s) 488
TaaI ACNGT 1 cut(s) 120
TaqI TCGA 3 cut(s) 24, 95, 125
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 1 cut(s) 133
TseI GCWGC 3 cut(s) 13, 60, 381
TspDTI ATGAA 1 cut(s) 24
Tth111I GACNNNGTC 2 cut(s) 7, 181
VpaK11BI GGWCC 1 cut(s) 474
XspI CTAG 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.