Rmu_sc0001250.1_g000045

Actin-depolymerizing factor 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001250.1
N/A
Physical Location & Seq
Forward (+)
222862 .. 229482
6621 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 843 bp
atgggtggacgcgaacgcaggggcgctgggtgcagggctgtaggcagcgaggctaggctgaagctgatggaggccgtgactggggtttggagggagaaccgctggcagaggcagcagcgacgagatcgatgtcgaggtgcgcggtggagcgatctggaaaggatttccagcggttggggatcacaggtggttggggtgcttcgtcgaaagcttcccagattggctgcaacatggagtcgtggtggtgctgttacatgcgtctattgtgctgaggaggtggagattgggcttttccttggctcctatgggctgcttttgatttccagcggttggggatcacaggtggtcggggtgcttcgtcgaaagcttcccagattggcagcaacatggagtcgtggtggtgctgttacatgcgtctattgtgctgaggaggccaatgcagcttctggaatggctgtgcacgatgactgcaagctgaggttcttggagctaaaggcaaagaggagctatcgtttcattttgttcaagattgagcaacagcaaattgtggttgatacgctcggggaacccaatgcaagttacgacgatttcacctccagttttcctgctgatgagtgtcgttatgctgtctatgatttcgatttcactaccgaagagaactgccagaaaagcaagattttcttcatcgcatggtcaccggatacatcaaaggtgaggatgaagatggtgtatgcaagttccaaggacagattcaaaagagaattggacggcatttcatttgaattgcaagcaacagatccaagtgagatgagcttggacatagtgaaagggcgagcctgctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

280

Amino Acids

31.57

Weight (kDa)

9.18

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 150 - 278 4.7e-40 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 174, 330
AccII CGCG 2 cut(s) 12, 142
AciI CCGC 4 cut(s) 100, 142, 171, 327
AclWI GGATC 3 cut(s) 187, 343, 791
AcuI CTGAAG 1 cut(s) 80
AfiI CCNNNNNNNGG 3 cut(s) 81, 174, 330
AgsI TTSAA 3 cut(s) 526, 754, 782
AjuI GAANNNNNNNTTGG 2 cut(s) 734, 766
AluBI AGCT 8 cut(s) 64, 211, 367, 443, 475, 490, 507, 813
AluI AGCT 8 cut(s) 64, 211, 367, 443, 475, 490, 507, 813
Alw21I GWGCWC 1 cut(s) 462
Alw44I GTGCAC 1 cut(s) 458
AlwI GGATC 3 cut(s) 187, 343, 791
AlwNI CAGNNNCTG 1 cut(s) 446
Ama87I CYCGRG 1 cut(s) 560
AoxI GGCC 2 cut(s) 72, 432
ApaLI GTGCAC 1 cut(s) 458
ApeKI GCWGC 7 cut(s) 45, 112, 115, 224, 310, 380, 440
AspLEI GCGC 2 cut(s) 26, 142
AsuHPI GGTGA 3 cut(s) 583, 687, 724
AvaI CYCGRG 1 cut(s) 560
BaeGI GKGCMC 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 462
BbvCI CCTCAGC 3 cut(s) 270, 426, 476
BbvI GCAGC 7 cut(s) 57, 124, 127, 211, 297, 392, 452
BccI CCATC 2 cut(s) 61, 718
BceAI ACGGC 2 cut(s) 59, 784
BciVI GTATCC 1 cut(s) 694
BfaI CTAG 1 cut(s) 54
BfmI CTRYAG 1 cut(s) 39
BfoI RGCGCY 1 cut(s) 27
BfuI GTATCC 1 cut(s) 694
BisI GCNGC 7 cut(s) 46, 113, 116, 225, 311, 381, 441
BlsI GCNGC 7 cut(s) 47, 114, 117, 226, 312, 382, 442
BmeT110I CYCGRG 1 cut(s) 560
BmiI GGNNCC 2 cut(s) 301, 567
BmrI ACTGGG 1 cut(s) 90
BmuI ACTGGG 1 cut(s) 90
BpmI CTGGAG 1 cut(s) 580
Bpu10I CCTNAGC 3 cut(s) 270, 426, 476
Bsa29I ATCGAT 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 295, 741
BsaWI WCCGGW 1 cut(s) 697
BsaXI ACNNNNNCTCC 6 cut(s) 226, 256, 382, 412, 496, 526
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 174, 330
Bse1I ACTGG 2 cut(s) 85, 597
BseCI ATCGAT 1 cut(s) 127
BseDI CCNNGG 2 cut(s) 295, 741
BseGI GGATG 1 cut(s) 723
BseLI CCNNNNNNNGG 3 cut(s) 81, 174, 330
BseMII CTCAG 3 cut(s) 261, 417, 467
BseNI ACTGG 2 cut(s) 85, 597
BseRI GAGGAG 3 cut(s) 287, 443, 517
BseSI GKGCMC 1 cut(s) 462
BseXI GCAGC 7 cut(s) 57, 124, 127, 211, 297, 392, 452
BseYI CCCAGC 1 cut(s) 26
BsgI GTGCAG 1 cut(s) 52
Bsh1236I CGCG 2 cut(s) 12, 142
BshFI GGCC 2 cut(s) 74, 434
BshVI ATCGAT 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 462
BsiHKCI CYCGRG 1 cut(s) 560
BsiSI CCGG 1 cut(s) 698
BslI CCNNNNNNNGG 3 cut(s) 81, 174, 330
BsnI GGCC 2 cut(s) 74, 434
BsoBI CYCGRG 1 cut(s) 560
Bsp1286I GDGCHC 1 cut(s) 462
Bsp143I GATC 5 cut(s) 124, 151, 179, 335, 796
BspACI CCGC 4 cut(s) 100, 142, 171, 327
BspANI GGCC 2 cut(s) 74, 434
BspCNI CTCAG 3 cut(s) 262, 418, 468
BspDI ATCGAT 1 cut(s) 127
BspFNI CGCG 2 cut(s) 12, 142
BspLI GGNNCC 2 cut(s) 301, 567
BspPI GGATC 3 cut(s) 187, 343, 791
BsrI ACTGG 2 cut(s) 85, 597
BssECI CCNNGG 2 cut(s) 295, 741
BssMI GATC 5 cut(s) 124, 151, 179, 335, 796
BssT1I CCWWGG 2 cut(s) 295, 741
Bst6I CTCTTC 1 cut(s) 648
BstC8I GCNNGC 5 cut(s) 104, 473, 789, 834, 838
BstDEI CTNAG 3 cut(s) 270, 426, 476
BstEII GGTNACC 1 cut(s) 693
BstF5I GGATG 1 cut(s) 723
BstFNI CGCG 2 cut(s) 12, 142
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 2 cut(s) 26, 142
BstKTI GATC 5 cut(s) 127, 154, 182, 338, 799
BstMBI GATC 5 cut(s) 124, 151, 179, 335, 796
BstMWI GCNNNNNNNGC 5 cut(s) 30, 112, 431, 440, 669
BstNSI RCATGY 2 cut(s) 258, 414
BstPI GGTNACC 1 cut(s) 693
BstSFI CTRYAG 1 cut(s) 39
BstSLI GKGCMC 1 cut(s) 462
BstUI CGCG 2 cut(s) 12, 142
BstV1I GCAGC 7 cut(s) 57, 124, 127, 211, 297, 392, 452
BstX2I RGATCY 1 cut(s) 796
BstYI RGATCY 1 cut(s) 796
Bsu15I ATCGAT 1 cut(s) 127
BsuI GTATCC 1 cut(s) 694
BsuRI GGCC 2 cut(s) 74, 434
BsuTUI ATCGAT 1 cut(s) 127
BtgZI GCGATG 1 cut(s) 670
BtsCI GGATG 1 cut(s) 723
Cac8I GCNNGC 5 cut(s) 104, 473, 789, 834, 838
CaiI CAGNNNCTG 1 cut(s) 446
CfoI GCGC 2 cut(s) 26, 142
ClaI ATCGAT 1 cut(s) 127
CseI GACGC 3 cut(s) 18, 247, 403
CviAII CATG 5 cut(s) 231, 255, 387, 411, 690
DdeI CTNAG 3 cut(s) 270, 426, 476
DpnI GATC 5 cut(s) 126, 153, 181, 337, 798
DpnII GATC 5 cut(s) 124, 151, 179, 335, 796
Eam1104I CTCTTC 1 cut(s) 648
EarI CTCTTC 1 cut(s) 648
Eco130I CCWWGG 2 cut(s) 295, 741
Eco57I CTGAAG 1 cut(s) 80
Eco88I CYCGRG 1 cut(s) 560
Eco91I GGTNACC 1 cut(s) 693
EcoO65I GGTNACC 1 cut(s) 693
EcoT14I CCWWGG 2 cut(s) 295, 741
ErhI CCWWGG 2 cut(s) 295, 741
FaeI CATG 5 cut(s) 234, 258, 390, 414, 693
FalI AAGNNNNNCTT 2 cut(s) 665, 697
FatI CATG 5 cut(s) 230, 254, 386, 410, 689
Fnu4HI GCNGC 7 cut(s) 46, 113, 116, 225, 311, 381, 441
FokI GGATG 1 cut(s) 730
Fsp4HI GCNGC 7 cut(s) 46, 113, 116, 225, 311, 381, 441
FspBI CTAG 1 cut(s) 54
GlaI GCGC 2 cut(s) 25, 141
GluI GCNGC 7 cut(s) 46, 113, 116, 225, 311, 381, 441
GsaI CCCAGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 580
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 2 cut(s) 74, 434
HapII CCGG 1 cut(s) 698
HgaI GACGC 3 cut(s) 18, 247, 403
HhaI GCGC 2 cut(s) 26, 142
Hin1II CATG 5 cut(s) 234, 258, 390, 414, 693
Hin6I GCGC 2 cut(s) 24, 140
HinP1I GCGC 2 cut(s) 24, 140
HindIII AAGCTT 2 cut(s) 209, 365
HinfI GANTC 3 cut(s) 235, 391, 750
HpaII CCGG 1 cut(s) 698
HphI GGTGA 3 cut(s) 583, 687, 724
Hpy166II GTNNAC 2 cut(s) 8, 460
Hpy188III TCNNGA 3 cut(s) 155, 447, 526
Hpy8I GTNNAC 2 cut(s) 8, 460
Hpy99I CGWCG 4 cut(s) 123, 207, 363, 587
HpyCH4V TGCA 8 cut(s) 33, 227, 440, 460, 471, 575, 734, 787
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 112, 431, 440, 669
HpyF3I CTNAG 3 cut(s) 270, 426, 476
Hsp92II CATG 5 cut(s) 234, 258, 390, 414, 693
HspAI GCGC 2 cut(s) 24, 140
Kzo9I GATC 5 cut(s) 124, 151, 179, 335, 796
LmnI GCTCC 4 cut(s) 147, 305, 487, 504
Lsp1109I GCAGC 7 cut(s) 57, 124, 127, 211, 297, 392, 452
MaeI CTAG 1 cut(s) 54
MaeIII GTNAC 5 cut(s) 76, 250, 406, 578, 693
MalI GATC 5 cut(s) 126, 153, 181, 337, 798
MboI GATC 5 cut(s) 124, 151, 179, 335, 796
MboII GAAGA 3 cut(s) 665, 673, 733
MflI RGATCY 1 cut(s) 796
MhlI GDGCHC 1 cut(s) 462
MluCI AATT 3 cut(s) 543, 761, 782
MlyI GAGTC 2 cut(s) 244, 400
MspA1I CMGCKG 3 cut(s) 102, 171, 327
MspI CCGG 1 cut(s) 698
MvnI CGCG 2 cut(s) 12, 142
MwoI GCNNNNNNNGC 5 cut(s) 30, 112, 431, 440, 669
NdeII GATC 5 cut(s) 124, 151, 179, 335, 796
NlaIII CATG 5 cut(s) 234, 258, 390, 414, 693
NlaIV GGNNCC 2 cut(s) 301, 567
NmuCI GTSAC 2 cut(s) 76, 693
NspI RCATGY 2 cut(s) 258, 414
PfeI GAWTC 1 cut(s) 750
PflMI CCANNNNNTGG 2 cut(s) 174, 330
PkrI GCNGC 7 cut(s) 47, 114, 117, 226, 312, 382, 442
PleI GAGTC 2 cut(s) 243, 399
PpsI GAGTC 2 cut(s) 243, 399
PspEI GGTNACC 1 cut(s) 693
PspFI CCCAGC 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 301, 567
PstNI CAGNNNCTG 1 cut(s) 446
PsuI RGATCY 1 cut(s) 796
SatI GCNGC 7 cut(s) 46, 113, 116, 225, 311, 381, 441
Sau3AI GATC 5 cut(s) 124, 151, 179, 335, 796
SchI GAGTC 2 cut(s) 244, 400
SduI GDGCHC 1 cut(s) 462
SfcI CTRYAG 1 cut(s) 39
Sse9I AATT 3 cut(s) 543, 761, 782
SsiI CCGC 4 cut(s) 100, 142, 171, 327
SspMI CTAG 1 cut(s) 54
StyI CCWWGG 2 cut(s) 295, 741
TaqI TCGA 5 cut(s) 127, 133, 205, 361, 639
TasI AATT 3 cut(s) 543, 761, 782
TfiI GAWTC 1 cut(s) 750
TseFI GTSAC 2 cut(s) 76, 693
TseI GCWGC 7 cut(s) 45, 112, 115, 224, 310, 380, 440
Tsp45I GTSAC 2 cut(s) 76, 693
TspDTI ATGAA 4 cut(s) 505, 673, 734, 765
Van91I CCANNNNNTGG 2 cut(s) 174, 330
VneI GTGCAC 1 cut(s) 458
XceI RCATGY 2 cut(s) 258, 414
XspI CTAG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.