Rmu_sc0001350.1_g000027

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001350.1
N/A
Physical Location & Seq
Reverse (-)
160675 .. 161145
471 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
atgttcgccagcatctcaaggccgatggaatcctggatacgattcatgagcctagccaggacgtgctatctgttaagcaaactcaagctttggaagcaaatagctagagcagcctttgaggcaaacaaggcgaaagccatcatcctaatgactcatcatatggatgattcactccagtgcgagtgtatgaatgaagaagaccccagaaggttgtgggtctcactcgacgaaagatttgacaacgtctgtgactccctgcttcttgacctagaagtgagatggcataacctccgcttatgtgatttcaagtcagttcttgactacaactcgaaaacacttcgcattaaatccataatggatttctttggtaaagagatcacagatgcgatgttgattgagaagactctctctaccttccccatttatgcattcatggtcgctaagaactatcgaattgatgttactacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

156

Amino Acids

18.43

Weight (kDa)

7.63

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 35 - 140 4.5e-06 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 292
AgsI TTSAA 1 cut(s) 307
AjiI CACGTC 1 cut(s) 63
AjnI CCWGG 2 cut(s) 32, 56
AleI CACNNNNGTG 1 cut(s) 175
AluBI AGCT 2 cut(s) 88, 104
AluI AGCT 2 cut(s) 88, 104
Alw26I GTCTC 1 cut(s) 223
AoxI GGCC 1 cut(s) 20
ApeKI GCWGC 1 cut(s) 110
ArsI GACNNNNNNTTYG 2 cut(s) 218, 250
BbsI GAAGAC 2 cut(s) 204, 407
BbvI GCAGC 1 cut(s) 122
BccI CCATC 3 cut(s) 19, 146, 273
BciT130I CCWGG 2 cut(s) 34, 58
BciVI GTATCC 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 223
BfaI CTAG 3 cut(s) 53, 105, 269
BfuI GTATCC 1 cut(s) 30
BglI GCCNNNNNGGC 1 cut(s) 119
BisI GCNGC 1 cut(s) 111
BlsI GCNGC 1 cut(s) 112
Bme1390I CCNGG 2 cut(s) 34, 58
BmgBI CACGTC 1 cut(s) 63
BmrFI CCNGG 2 cut(s) 34, 58
BmsI GCATC 2 cut(s) 21, 373
BpiI GAAGAC 2 cut(s) 204, 407
BpmI CTGGAG 1 cut(s) 158
BpuEI CTTGAG 1 cut(s) 68
BsaI GGTCTC 1 cut(s) 223
Bse1I ACTGG 1 cut(s) 175
BseBI CCWGG 2 cut(s) 34, 58
BseGI GGATG 2 cut(s) 141, 169
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 1 cut(s) 122
BshFI GGCC 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 223
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 1 cut(s) 22
Bso31I GGTCTC 1 cut(s) 223
Bsp143I GATC 1 cut(s) 375
BspACI CCGC 1 cut(s) 292
BspANI GGCC 1 cut(s) 22
BspHI TCATGA 1 cut(s) 45
BspTNI GGTCTC 1 cut(s) 223
BsrI ACTGG 1 cut(s) 175
BssMI GATC 1 cut(s) 375
Bst2UI CCWGG 2 cut(s) 34, 58
BstC8I GCNNGC 1 cut(s) 10
BstDEI CTNAG 1 cut(s) 441
BstF5I GGATG 2 cut(s) 141, 169
BstKTI GATC 1 cut(s) 378
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 1 cut(s) 375
BstMWI GCNNNNNNNGC 4 cut(s) 94, 110, 119, 128
BstNI CCWGG 2 cut(s) 34, 58
BstSCI CCNGG 2 cut(s) 32, 56
BstV1I GCAGC 1 cut(s) 122
BstV2I GAAGAC 2 cut(s) 204, 407
BsuI GTATCC 1 cut(s) 30
BsuRI GGCC 1 cut(s) 22
BtgZI GCGATG 1 cut(s) 401
BtrI CACGTC 1 cut(s) 63
BtsCI GGATG 2 cut(s) 141, 169
BtsIMutI CAGTG 1 cut(s) 182
Cac8I GCNNGC 1 cut(s) 10
CciI TCATGA 1 cut(s) 45
CviAII CATG 3 cut(s) 46, 433, 468
CviJI RGCY 7 cut(s) 22, 51, 56, 88, 104, 113, 137
CviKI_1 RGCY 7 cut(s) 22, 51, 56, 88, 104, 113, 137
DdeI CTNAG 1 cut(s) 441
DpnI GATC 1 cut(s) 377
DpnII GATC 1 cut(s) 375
Eco31I GGTCTC 1 cut(s) 223
EcoRII CCWGG 2 cut(s) 32, 56
EcoT22I ATGCAT 1 cut(s) 430
FaeI CATG 3 cut(s) 49, 436, 471
FatI CATG 3 cut(s) 45, 432, 467
FauNDI CATATG 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 111
FokI GGATG 2 cut(s) 128, 176
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 3 cut(s) 53, 105, 269
GluI GCNGC 1 cut(s) 111
GsuI CTGGAG 1 cut(s) 158
HaeIII GGCC 1 cut(s) 22
Hin1II CATG 3 cut(s) 49, 436, 471
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 6 cut(s) 29, 42, 151, 167, 251, 403
Hpy188III TCNNGA 3 cut(s) 46, 263, 317
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 2 cut(s) 201, 424
HpyCH4IV ACGT 2 cut(s) 62, 243
HpyCH4V TGCA 1 cut(s) 428
HpyF10VI GCNNNNNNNGC 4 cut(s) 94, 110, 119, 128
HpyF3I CTNAG 1 cut(s) 441
HpySE526I ACGT 2 cut(s) 62, 243
Hsp92II CATG 3 cut(s) 49, 436, 471
Kzo9I GATC 1 cut(s) 375
LpnPI CCDG 8 cut(s) 19, 22, 43, 46, 70, 188, 217, 269
Lsp1109I GCAGC 1 cut(s) 122
LweI GCATC 2 cut(s) 21, 373
MaeI CTAG 3 cut(s) 53, 105, 269
MaeII ACGT 2 cut(s) 62, 243
MaeIII GTNAC 2 cut(s) 248, 460
MalI GATC 1 cut(s) 377
MboI GATC 1 cut(s) 375
MboII GAAGA 3 cut(s) 206, 209, 412
MluCI AATT 1 cut(s) 453
MlyI GAGTC 3 cut(s) 145, 245, 397
MnlI CCTC 2 cut(s) 112, 299
Mph1103I ATGCAT 1 cut(s) 430
MseI TTAA 2 cut(s) 74, 345
MslI CAYNNNNRTG 3 cut(s) 146, 162, 175
MspR9I CCNGG 2 cut(s) 34, 58
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 2 cut(s) 34, 58
MwoI GCNNNNNNNGC 4 cut(s) 94, 110, 119, 128
NdeI CATATG 1 cut(s) 159
NdeII GATC 1 cut(s) 375
NlaIII CATG 3 cut(s) 49, 436, 471
NmuCI GTSAC 1 cut(s) 248
NsiI ATGCAT 1 cut(s) 430
OliI CACNNNNGTG 1 cut(s) 175
PagI TCATGA 1 cut(s) 45
PctI GAATGC 1 cut(s) 428
PfeI GAWTC 3 cut(s) 29, 42, 167
PflFI GACNNNGTC 1 cut(s) 242
PfoI TCCNGGA 1 cut(s) 32
PkrI GCNGC 1 cut(s) 112
PleI GAGTC 3 cut(s) 145, 245, 397
PpsI GAGTC 3 cut(s) 145, 245, 397
Psp6I CCWGG 2 cut(s) 32, 56
PspGI CCWGG 2 cut(s) 32, 56
PsyI GACNNNGTC 1 cut(s) 242
RseI CAYNNNNRTG 3 cut(s) 146, 162, 175
SaqAI TTAA 2 cut(s) 74, 345
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 1 cut(s) 375
SchI GAGTC 3 cut(s) 145, 245, 397
ScrFI CCNGG 2 cut(s) 34, 58
SetI ASST 8 cut(s) 65, 90, 106, 212, 246, 270, 291, 416
SfaNI GCATC 2 cut(s) 21, 373
SmiMI CAYNNNNRTG 3 cut(s) 146, 162, 175
SmlI CTYRAG 2 cut(s) 16, 83
SmoI CTYRAG 2 cut(s) 16, 83
Sse9I AATT 1 cut(s) 453
SsiI CCGC 1 cut(s) 292
SspMI CTAG 3 cut(s) 53, 105, 269
StyD4I CCNGG 2 cut(s) 32, 56
TaiI ACGT 2 cut(s) 65, 246
TaqI TCGA 3 cut(s) 225, 329, 451
TasI AATT 1 cut(s) 453
TfiI GAWTC 3 cut(s) 29, 42, 167
Tru1I TTAA 2 cut(s) 74, 345
Tru9I TTAA 2 cut(s) 74, 345
TscAI CASTG 1 cut(s) 182
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 1 cut(s) 110
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 4 cut(s) 34, 203, 207, 421
TspRI CASTG 1 cut(s) 182
Tth111I GACNNNGTC 1 cut(s) 242
XspI CTAG 3 cut(s) 53, 105, 269
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.