Rmu_sc0001355.1_g000001

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001355.1
N/A
Physical Location & Seq
Forward (+)
1 .. 2937
2937 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1120 bp
caacttctcttgtggagggttgcctccagtagcttctttacagaagcatgagacttccccaggtcagaatggagatctcaggtctccgaagagaccctgcacagaatataggggtgtattactggagcgaaattcctttacttctccagtgaaggatcgtctcctggccttaagtaaccttaaatcaaagctgccaccgccacctctacaatctgcatttgcaagtccaacacgaccaaatccagcaggtggaggggaaacatgtgcagaaactggagttagtattttctttaataagatcatcaagctggctgctgtaagaattaatggtatggtcgaaaggttgcaattgtctcagcaaatcagggagaatgtttatcgcctcttccaacaaatacttgttcagcggacatctctattttttaaccgccatattgaccaaattatcctttgttgtttttacggggttgccaagatttctcaactaagtcttacatttagggaaatcatacacaactacaggaagcaaccacaatgcaaaccacaagtttttcgaagtgtctttgttgactggtcatcagctagacgcaatgggcggcctggacaggaacacgtagatatcattagattttacaatgaagtattcattccttctgtcaagaccctgcttgttgagcttgggccagctgttacaactacaagagccaatgtagttcctgaagctaacaacaacaatgatgctcaatgtccaggatcacctaaagtatccacctttccaagtctccctgatatgtctccaaagaaagtatctgcagcacacaatgtttatgtatctccattgcgatcatcaaagatggatgccttaatctcacatagctcaaagagctattatgcttgtgttggggagagtactcatgcgtatcagagcccttcaaaagatctgacagccattaacaaccgcttgaatggcacacgaaaacgcacactcgactttgataatgtggatgtaggggttggtttggttagtgattctatggtggccaatagcttgtaccttcaaaatgggagttgtgcatcctcatcgggtggaccattgaaaaccgagcaacctgacttgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

372

Amino Acids

40.95

Weight (kDa)

9.51

Isoelectric Point (pI)

59.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RB_B PF01857 93 - 220 3.9e-44 Retinoblastoma-associated protein B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 237
Acc36I ACCTGC 1 cut(s) 237
AccB7I CCANNNNNTGG 1 cut(s) 249
AciI CCGC 5 cut(s) 198, 407, 428, 596, 959
AclWI GGATC 2 cut(s) 163, 761
AcoI YGGCCR 1 cut(s) 1039
AcsI RAATTY 1 cut(s) 131
AcuI CTGAAG 1 cut(s) 739
AfaI GTAC 2 cut(s) 911, 1053
AfiI CCNNNNNNNGG 1 cut(s) 249
AflII CTTAAG 1 cut(s) 170
AflIII ACRYGT 2 cut(s) 261, 611
AgsI TTSAA 4 cut(s) 934, 965, 1059, 1097
AjnI CCWGG 4 cut(s) 59, 163, 599, 749
Alw26I GTCTC 7 cut(s) 45, 86, 88, 165, 358, 786, 799
AlwI GGATC 2 cut(s) 163, 761
AlwNI CAGNNNCTG 1 cut(s) 273
AoxI GGCC 4 cut(s) 166, 597, 681, 1039
ApeKI GCWGC 3 cut(s) 191, 312, 813
ApoI RAATTY 1 cut(s) 131
AseI ATTAAT 1 cut(s) 325
AspS9I GGNCC 2 cut(s) 681, 1089
AsuHPI GGTGA 1 cut(s) 748
AsuII TTCGAA 1 cut(s) 554
AvaII GGWCC 1 cut(s) 1089
BalI TGGCCA 1 cut(s) 1041
BanII GRGCYC 1 cut(s) 930
BbvI GCAGC 3 cut(s) 178, 299, 825
BccI CCATC 1 cut(s) 848
BcgI CGANNNNNNTGC 2 cut(s) 1063, 1097
BciT130I CCWGG 4 cut(s) 61, 165, 601, 751
BciVI GTATCC 1 cut(s) 776
BcoDI GTCTC 7 cut(s) 45, 86, 88, 165, 358, 786, 799
BfaI CTAG 1 cut(s) 583
BfmI CTRYAG 2 cut(s) 518, 811
BfrI CTTAAG 1 cut(s) 170
BfuAI ACCTGC 1 cut(s) 237
BfuI GTATCC 1 cut(s) 776
BglII AGATCT 2 cut(s) 74, 938
BisI GCNGC 4 cut(s) 192, 313, 597, 814
BlsI GCNGC 4 cut(s) 193, 314, 598, 815
BmcAI AGTACT 1 cut(s) 911
Bme1390I CCNGG 4 cut(s) 61, 165, 601, 751
Bme18I GGWCC 1 cut(s) 1089
BmgT120I GGNCC 2 cut(s) 681, 1089
BmrFI CCNGG 4 cut(s) 61, 165, 601, 751
BmsI GCATC 3 cut(s) 728, 848, 1083
BpmI CTGGAG 4 cut(s) 10, 130, 144, 295
Bpu14I TTCGAA 1 cut(s) 554
BsaAI YACGTR 1 cut(s) 614
BsaI GGTCTC 2 cut(s) 86, 88
BsaJI CCNNGG 1 cut(s) 59
Bsc4I CCNNNNNNNGG 1 cut(s) 249
Bse1I ACTGG 5 cut(s) 27, 127, 147, 278, 576
Bse3DI GCAATG 2 cut(s) 596, 837
BseBI CCWGG 4 cut(s) 61, 165, 601, 751
BseDI CCNNGG 1 cut(s) 59
BseGI GGATG 3 cut(s) 863, 1010, 1074
BseLI CCNNNNNNNGG 1 cut(s) 249
BseMI GCAATG 2 cut(s) 596, 837
BseMII CTCAG 2 cut(s) 92, 369
BseNI ACTGG 5 cut(s) 27, 127, 147, 278, 576
BseXI GCAGC 3 cut(s) 178, 299, 825
BsgI GTGCAG 2 cut(s) 83, 286
BshFI GGCC 4 cut(s) 168, 599, 683, 1041
BslI CCNNNNNNNGG 1 cut(s) 249
BsmAI GTCTC 7 cut(s) 45, 86, 88, 165, 358, 786, 799
BsmBI CGTCTC 1 cut(s) 165
BsnI GGCC 4 cut(s) 168, 599, 683, 1041
Bso31I GGTCTC 2 cut(s) 86, 88
Bsp119I TTCGAA 1 cut(s) 554
Bsp1286I GDGCHC 1 cut(s) 930
Bsp143I GATC 6 cut(s) 74, 155, 298, 753, 843, 938
BspACI CCGC 5 cut(s) 198, 407, 428, 596, 959
BspANI GGCC 4 cut(s) 168, 599, 683, 1041
BspCNI CTCAG 2 cut(s) 91, 368
BspMAI CTGCAG 1 cut(s) 815
BspMI ACCTGC 1 cut(s) 237
BspPI GGATC 2 cut(s) 163, 761
BspT104I TTCGAA 1 cut(s) 554
BspTI CTTAAG 1 cut(s) 170
BspTNI GGTCTC 2 cut(s) 86, 88
BsrDI GCAATG 2 cut(s) 596, 837
BsrI ACTGG 5 cut(s) 27, 127, 147, 278, 576
BssECI CCNNGG 1 cut(s) 59
BssMI GATC 6 cut(s) 74, 155, 298, 753, 843, 938
Bst2UI CCWGG 4 cut(s) 61, 165, 601, 751
Bst6I CTCTTC 2 cut(s) 84, 390
BstAFI CTTAAG 1 cut(s) 170
BstBAI YACGTR 1 cut(s) 614
BstBI TTCGAA 1 cut(s) 554
BstC8I GCNNGC 2 cut(s) 310, 685
BstDEI CTNAG 3 cut(s) 78, 355, 486
BstF5I GGATG 3 cut(s) 863, 1010, 1074
BstKTI GATC 6 cut(s) 77, 158, 301, 756, 846, 941
BstMAI GTCTC 7 cut(s) 45, 86, 88, 165, 358, 786, 799
BstMBI GATC 6 cut(s) 74, 155, 298, 753, 843, 938
BstMWI GCNNNNNNNGC 4 cut(s) 197, 674, 883, 967
BstNI CCWGG 4 cut(s) 61, 165, 601, 751
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 4 cut(s) 59, 163, 599, 749
BstSFI CTRYAG 2 cut(s) 518, 811
BstV1I GCAGC 3 cut(s) 178, 299, 825
BstX2I RGATCY 2 cut(s) 74, 938
BstYI RGATCY 2 cut(s) 74, 938
BsuI GTATCC 1 cut(s) 776
BsuRI GGCC 4 cut(s) 168, 599, 683, 1041
BtsCI GGATG 3 cut(s) 863, 1010, 1074
BtsIMutI CAGTG 1 cut(s) 154
BveI ACCTGC 1 cut(s) 237
Cac8I GCNNGC 2 cut(s) 310, 685
CaiI CAGNNNCTG 1 cut(s) 273
Cfr13I GGNCC 2 cut(s) 681, 1089
CseI GACGC 1 cut(s) 595
Csp6I GTAC 2 cut(s) 910, 1052
CviAII CATG 3 cut(s) 48, 262, 915
CviQI GTAC 2 cut(s) 910, 1052
DdeI CTNAG 3 cut(s) 78, 355, 486
DpnI GATC 6 cut(s) 76, 157, 300, 755, 845, 940
DpnII GATC 6 cut(s) 74, 155, 298, 753, 843, 938
EaeI YGGCCR 1 cut(s) 1039
Eam1104I CTCTTC 2 cut(s) 84, 390
EarI CTCTTC 2 cut(s) 84, 390
Eco24I GRGCYC 1 cut(s) 930
Eco31I GGTCTC 2 cut(s) 86, 88
Eco32I GATATC 1 cut(s) 620
Eco47I GGWCC 1 cut(s) 1089
Eco57I CTGAAG 1 cut(s) 739
EcoRII CCWGG 4 cut(s) 59, 163, 599, 749
EcoRV GATATC 1 cut(s) 620
EcoT38I GRGCYC 1 cut(s) 930
Esp3I CGTCTC 1 cut(s) 165
FaeI CATG 3 cut(s) 51, 265, 918
FatI CATG 3 cut(s) 47, 261, 914
Fnu4HI GCNGC 4 cut(s) 192, 313, 597, 814
FokI GGATG 3 cut(s) 870, 1017, 1061
FriOI GRGCYC 1 cut(s) 930
Fsp4HI GCNGC 4 cut(s) 192, 313, 597, 814
FspBI CTAG 1 cut(s) 583
GluI GCNGC 4 cut(s) 192, 313, 597, 814
GsuI CTGGAG 4 cut(s) 10, 130, 144, 295
HaeIII GGCC 4 cut(s) 168, 599, 683, 1041
HgaI GACGC 1 cut(s) 595
Hin1II CATG 3 cut(s) 51, 265, 918
HincII GTYRAC 1 cut(s) 569
HindII GTYRAC 1 cut(s) 569
HinfI GANTC 1 cut(s) 1029
HphI GGTGA 1 cut(s) 748
Hpy166II GTNNAC 2 cut(s) 569, 1089
Hpy188I TCNGA 4 cut(s) 67, 88, 925, 943
Hpy188III TCNNGA 2 cut(s) 659, 717
Hpy8I GTNNAC 2 cut(s) 569, 1089
HpyAV CCTTC 4 cut(s) 146, 661, 940, 1065
HpyCH4IV ACGT 1 cut(s) 613
HpyCH4V TGCA 8 cut(s) 100, 216, 222, 267, 347, 538, 813, 1074
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 674, 883, 967
HpyF3I CTNAG 3 cut(s) 78, 355, 486
HpySE526I ACGT 1 cut(s) 613
Hsp92II CATG 3 cut(s) 51, 265, 918
Kzo9I GATC 6 cut(s) 74, 155, 298, 753, 843, 938
LmnI GCTCC 1 cut(s) 125
Lsp1109I GCAGC 3 cut(s) 178, 299, 825
LweI GCATC 3 cut(s) 728, 848, 1083
MaeI CTAG 1 cut(s) 583
MaeII ACGT 1 cut(s) 613
MaeIII GTNAC 2 cut(s) 174, 689
MalI GATC 6 cut(s) 76, 157, 300, 755, 845, 940
MboI GATC 6 cut(s) 74, 155, 298, 753, 843, 938
MboII GAAGA 2 cut(s) 101, 377
MfeI CAATTG 1 cut(s) 348
MflI RGATCY 2 cut(s) 74, 938
MhlI GDGCHC 1 cut(s) 930
MlsI TGGCCA 1 cut(s) 1041
MluCI AATT 4 cut(s) 131, 322, 348, 442
MluNI TGGCCA 1 cut(s) 1041
MmeI TCCRAC 2 cut(s) 252, 413
MnlI CCTC 6 cut(s) 9, 34, 214, 246, 393, 1088
Mox20I TGGCCA 1 cut(s) 1041
MscI TGGCCA 1 cut(s) 1041
MseI TTAA 7 cut(s) 171, 181, 292, 325, 424, 864, 952
Msp20I TGGCCA 1 cut(s) 1041
MspA1I CMGCKG 2 cut(s) 407, 687
MspCI CTTAAG 1 cut(s) 170
MspR9I CCNGG 4 cut(s) 61, 165, 601, 751
MunI CAATTG 1 cut(s) 348
MvaI CCWGG 4 cut(s) 61, 165, 601, 751
MwoI GCNNNNNNNGC 4 cut(s) 197, 674, 883, 967
NdeII GATC 6 cut(s) 74, 155, 298, 753, 843, 938
NlaIII CATG 3 cut(s) 51, 265, 918
NspI RCATGY 1 cut(s) 265
NspV TTCGAA 1 cut(s) 554
PaqCI CACCTGC 1 cut(s) 237
PciI ACATGT 1 cut(s) 261
PfeI GAWTC 1 cut(s) 1029
PflMI CCANNNNNTGG 1 cut(s) 249
PfoI TCCNGGA 1 cut(s) 749
PkrI GCNGC 4 cut(s) 193, 314, 598, 815
Ppu21I YACGTR 1 cut(s) 614
PscI ACATGT 1 cut(s) 261
PshBI ATTAAT 1 cut(s) 325
Psp6I CCWGG 4 cut(s) 59, 163, 599, 749
PspGI CCWGG 4 cut(s) 59, 163, 599, 749
PspPI GGNCC 2 cut(s) 681, 1089
PstI CTGCAG 1 cut(s) 815
PstNI CAGNNNCTG 1 cut(s) 273
PsuI RGATCY 2 cut(s) 74, 938
PvuII CAGCTG 1 cut(s) 687
RsaI GTAC 2 cut(s) 911, 1053
RsaNI GTAC 2 cut(s) 910, 1052
SaqAI TTAA 7 cut(s) 171, 181, 292, 325, 424, 864, 952
SatI GCNGC 4 cut(s) 192, 313, 597, 814
Sau3AI GATC 6 cut(s) 74, 155, 298, 753, 843, 938
Sau96I GGNCC 2 cut(s) 681, 1089
ScaI AGTACT 1 cut(s) 911
ScrFI CCNGG 4 cut(s) 61, 165, 601, 751
SduI GDGCHC 1 cut(s) 930
SfaNI GCATC 3 cut(s) 728, 848, 1083
SfcI CTRYAG 2 cut(s) 518, 811
SfuI TTCGAA 1 cut(s) 554
SinI GGWCC 1 cut(s) 1089
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 4 cut(s) 131, 322, 348, 442
SsiI CCGC 5 cut(s) 198, 407, 428, 596, 959
SspMI CTAG 1 cut(s) 583
StyD4I CCNGG 4 cut(s) 59, 163, 599, 749
TaiI ACGT 1 cut(s) 616
TaqI TCGA 3 cut(s) 337, 554, 988
TasI AATT 4 cut(s) 131, 322, 348, 442
TatI WGTACW 1 cut(s) 909
TauI GCSGC 1 cut(s) 599
TfiI GAWTC 1 cut(s) 1029
Tru1I TTAA 7 cut(s) 171, 181, 292, 325, 424, 864, 952
Tru9I TTAA 7 cut(s) 171, 181, 292, 325, 424, 864, 952
TscAI CASTG 1 cut(s) 154
TseI GCWGC 3 cut(s) 191, 312, 813
TspDTI ATGAA 2 cut(s) 635, 652
TspRI CASTG 1 cut(s) 154
Van91I CCANNNNNTGG 1 cut(s) 249
Vha464I CTTAAG 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 1089
VspI ATTAAT 1 cut(s) 325
XapI RAATTY 1 cut(s) 131
XceI RCATGY 1 cut(s) 265
XspI CTAG 1 cut(s) 583
ZrmI AGTACT 1 cut(s) 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.