Rmu_sc0001359.1_g000015

Transcription elongation factor B polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001359.1
N/A
Physical Location & Seq
Reverse (-)
79297 .. 82283
2987 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 798 bp
atgttggctctaaacttcaagtttcttgatcaggtctttccccactgcaccaaagaccagttgattcacatcaagaatagcacaaaaggtacagacctgactccgatcaccaataagctctggaataagttctttgagacagagttcgatggcaaagccactgatgagcttttttcagaagctgctgtggcttccttagataagcagaagctgaagaatgcagcttccagaagccagaagccacggaaacactgtagcggtagttactgtagattaatgaaaaatccaaaatgccgaaaattccccttccaagccaaaacaatttcagctgaaccattcgttaaccctacgctcatatcttcctcgtcactgttccccatctctctccctcatcacacccgcatcgatctacccgtctcgctgctctctcaccgctgcatcctcgctgcttcctcaccgccgcatcgtcactgcaccccatctccgttagtcatcagagaccgtagccccgacgtcgatctacccatctatacaaactccgcatcaatctacccatctctctgtcccttgtactgttggttcactgctctcttcctcccccgtctcccagatctgcagattccgtcgagctccttgctgagtgaagaaagagaatgtgatgggtcaagtggtgggatagagatggatgatgatgtagaagaagaagaatatcatggtcatggtgcacaagaaatggaaacaataagaaatagcattactcaaagtttgatgagtgcgcgtaataacgtgaatatttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Elongin_A

Protein Analysis

265

Amino Acids

29.76

Weight (kDa)

6.79

Isoelectric Point (pI)

58.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 516
AccII CGCG 1 cut(s) 778
AciI CCGC 6 cut(s) 258, 400, 433, 458, 461, 540
AcsI RAATTY 1 cut(s) 299
AcuI CTGAAG 1 cut(s) 233
AcyI GRCGYC 1 cut(s) 513
AfaI GTAC 2 cut(s) 91, 572
AgsI TTSAA 1 cut(s) 19
AluBI AGCT 7 cut(s) 118, 169, 182, 211, 224, 329, 630
AluI AGCT 7 cut(s) 118, 169, 182, 211, 224, 329, 630
Alw21I GWGCWC 2 cut(s) 632, 727
Alw26I GTCTC 4 cut(s) 131, 421, 492, 608
Alw44I GTGCAC 1 cut(s) 723
AlwNI CAGNNNCTG 2 cut(s) 182, 211
ApaLI GTGCAC 1 cut(s) 723
ApeKI GCWGC 5 cut(s) 182, 221, 421, 435, 446
ApoI RAATTY 1 cut(s) 299
AseI ATTAAT 1 cut(s) 275
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 1 cut(s) 778
AsuHPI GGTGA 3 cut(s) 100, 422, 447
BaeGI GKGCMC 1 cut(s) 727
BanII GRGCYC 1 cut(s) 632
Bbv12I GWGCWC 2 cut(s) 632, 727
BbvI GCAGC 5 cut(s) 169, 233, 408, 422, 433
BccI CCATC 7 cut(s) 143, 386, 487, 533, 562, 653, 676
BcgI CGANNNNNNTGC 2 cut(s) 616, 650
BclI TGATCA 1 cut(s) 28
BcoDI GTCTC 4 cut(s) 131, 421, 492, 608
BfmI CTRYAG 3 cut(s) 253, 268, 614
BglII AGATCT 1 cut(s) 610
BisI GCNGC 6 cut(s) 183, 222, 422, 436, 447, 461
BlsI GCNGC 6 cut(s) 184, 223, 423, 437, 448, 462
BmsI GCATC 4 cut(s) 411, 447, 472, 551
Bsa29I ATCGAT 1 cut(s) 405
BsaBI GATNNNNATC 1 cut(s) 68
BsaHI GRCGYC 1 cut(s) 513
BsaI GGTCTC 1 cut(s) 492
BsaJI CCNNGG 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 466, 496
Bse1I ACTGG 1 cut(s) 58
Bse8I GATNNNNATC 1 cut(s) 68
BseCI ATCGAT 1 cut(s) 405
BseDI CCNNGG 1 cut(s) 242
BseGI GGATG 2 cut(s) 438, 691
BseJI GATNNNNATC 1 cut(s) 68
BseMII CTCAG 1 cut(s) 629
BseNI ACTGG 1 cut(s) 58
BseSI GKGCMC 1 cut(s) 727
BseXI GCAGC 5 cut(s) 169, 233, 408, 422, 433
BsgI GTGCAG 2 cut(s) 31, 457
Bsh1236I CGCG 1 cut(s) 778
BshVI ATCGAT 1 cut(s) 405
BsiHKAI GWGCWC 2 cut(s) 632, 727
BslFI GGGAC 1 cut(s) 549
BsmAI GTCTC 4 cut(s) 131, 421, 492, 608
BsmBI CGTCTC 2 cut(s) 421, 608
BsmFI GGGAC 1 cut(s) 549
BsmI GAATGC 1 cut(s) 223
Bso31I GGTCTC 1 cut(s) 492
Bsp1286I GDGCHC 2 cut(s) 632, 727
Bsp143I GATC 5 cut(s) 28, 105, 406, 517, 610
BspACI CCGC 6 cut(s) 258, 400, 433, 458, 461, 540
BspCNI CTCAG 1 cut(s) 630
BspDI ATCGAT 1 cut(s) 405
BspFNI CGCG 1 cut(s) 778
BspMAI CTGCAG 1 cut(s) 618
BspTNI GGTCTC 1 cut(s) 492
BsrI ACTGG 1 cut(s) 58
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 5 cut(s) 28, 105, 406, 517, 610
BssNI GRCGYC 1 cut(s) 513
Bst4CI ACNGT 5 cut(s) 254, 269, 372, 503, 575
Bst6I CTCTTC 1 cut(s) 596
BstACI GRCGYC 1 cut(s) 513
BstDEI CTNAG 2 cut(s) 196, 638
BstDSI CCRYGG 1 cut(s) 242
BstF5I GGATG 2 cut(s) 438, 691
BstFNI CGCG 1 cut(s) 778
BstHHI GCGC 1 cut(s) 778
BstKTI GATC 5 cut(s) 31, 108, 409, 520, 613
BstMAI GTCTC 4 cut(s) 131, 421, 492, 608
BstMBI GATC 5 cut(s) 28, 105, 406, 517, 610
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstSFI CTRYAG 3 cut(s) 253, 268, 614
BstSLI GKGCMC 1 cut(s) 727
BstUI CGCG 1 cut(s) 778
BstV1I GCAGC 5 cut(s) 169, 233, 408, 422, 433
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
Bsu15I ATCGAT 1 cut(s) 405
BsuTUI ATCGAT 1 cut(s) 405
BtgI CCRYGG 1 cut(s) 242
BtsCI GGATG 2 cut(s) 438, 691
BtsI GCAGTG 3 cut(s) 43, 469, 582
BtsIMutI CAGTG 6 cut(s) 43, 159, 250, 368, 469, 582
CaiI CAGNNNCTG 2 cut(s) 182, 211
CfoI GCGC 1 cut(s) 778
ClaI ATCGAT 1 cut(s) 405
Csp6I GTAC 2 cut(s) 90, 571
CviAII CATG 2 cut(s) 713, 719
CviQI GTAC 2 cut(s) 90, 571
DdeI CTNAG 2 cut(s) 196, 638
DpnI GATC 5 cut(s) 30, 107, 408, 519, 612
DpnII GATC 5 cut(s) 28, 105, 406, 517, 610
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
Ecl136II GAGCTC 1 cut(s) 630
Eco24I GRGCYC 1 cut(s) 632
Eco31I GGTCTC 1 cut(s) 492
Eco53kI GAGCTC 1 cut(s) 630
Eco57I CTGAAG 1 cut(s) 233
EcoICRI GAGCTC 1 cut(s) 630
EcoT38I GRGCYC 1 cut(s) 632
Esp3I CGTCTC 2 cut(s) 421, 608
FaeI CATG 2 cut(s) 716, 722
FaiI YATR 4 cut(s) 356, 531, 714, 720
FaqI GGGAC 1 cut(s) 549
FatI CATG 2 cut(s) 712, 718
FauI CCCGC 1 cut(s) 407
FbaI TGATCA 1 cut(s) 28
Fnu4HI GCNGC 6 cut(s) 183, 222, 422, 436, 447, 461
FokI GGATG 2 cut(s) 425, 698
FriOI GRGCYC 1 cut(s) 632
Fsp4HI GCNGC 6 cut(s) 183, 222, 422, 436, 447, 461
GlaI GCGC 1 cut(s) 777
GluI GCNGC 6 cut(s) 183, 222, 422, 436, 447, 461
HhaI GCGC 1 cut(s) 778
Hin1I GRCGYC 1 cut(s) 513
Hin1II CATG 2 cut(s) 716, 722
Hin6I GCGC 1 cut(s) 776
HinP1I GCGC 1 cut(s) 776
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 3 cut(s) 64, 100, 619
HpaI GTTAAC 1 cut(s) 343
HphI GGTGA 3 cut(s) 100, 422, 447
Hpy166II GTNNAC 3 cut(s) 343, 582, 725
Hpy188I TCNGA 3 cut(s) 105, 178, 497
Hpy188III TCNNGA 4 cut(s) 26, 73, 121, 228
Hpy8I GTNNAC 3 cut(s) 343, 582, 725
Hpy99I CGWCG 3 cut(s) 515, 518, 628
HpyAV CCTTC 1 cut(s) 316
HpyCH4III ACNGT 5 cut(s) 254, 269, 372, 503, 575
HpyCH4IV ACGT 2 cut(s) 513, 786
HpyCH4V TGCA 6 cut(s) 48, 221, 438, 474, 616, 725
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 2 cut(s) 196, 638
HpySE526I ACGT 2 cut(s) 513, 786
Hsp92I GRCGYC 1 cut(s) 513
Hsp92II CATG 2 cut(s) 716, 722
HspAI GCGC 1 cut(s) 776
Ksp22I TGATCA 1 cut(s) 28
KspAI GTTAAC 1 cut(s) 343
Kzo9I GATC 5 cut(s) 28, 105, 406, 517, 610
LmnI GCTCC 1 cut(s) 635
LpnPI CCDG 7 cut(s) 17, 71, 106, 110, 241, 248, 621
Lsp1109I GCAGC 5 cut(s) 169, 233, 408, 422, 433
LweI GCATC 4 cut(s) 411, 447, 472, 551
MaeII ACGT 2 cut(s) 513, 786
MaeIII GTNAC 3 cut(s) 263, 366, 467
MalI GATC 5 cut(s) 30, 107, 408, 519, 612
MboI GATC 5 cut(s) 28, 105, 406, 517, 610
MboII GAAGA 7 cut(s) 226, 351, 583, 656, 710, 713, 716
MflI RGATCY 1 cut(s) 610
MhlI GDGCHC 2 cut(s) 632, 727
MluCI AATT 2 cut(s) 299, 321
MlyI GAGTC 1 cut(s) 94
MnlI CCTC 5 cut(s) 373, 399, 452, 463, 605
MroXI GAANNNNTTC 1 cut(s) 128
MseI TTAA 2 cut(s) 275, 342
MslI CAYNNNNRTG 1 cut(s) 717
MspA1I CMGCKG 2 cut(s) 329, 435
Mva1269I GAATGC 1 cut(s) 223
MvnI CGCG 1 cut(s) 778
MwoI GCNNNNNNNGC 1 cut(s) 188
NdeII GATC 5 cut(s) 28, 105, 406, 517, 610
NlaIII CATG 2 cut(s) 716, 722
NmuCI GTSAC 2 cut(s) 366, 467
PcsI WCGNNNNNNNCGW 1 cut(s) 411
PctI GAATGC 1 cut(s) 223
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 64, 619
PkrI GCNGC 6 cut(s) 184, 223, 423, 437, 448, 462
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
PshBI ATTAAT 1 cut(s) 275
Psp124BI GAGCTC 1 cut(s) 632
PsrI GAACNNNNNNTAC 2 cut(s) 563, 595
PstI CTGCAG 1 cut(s) 618
PstNI CAGNNNCTG 2 cut(s) 182, 211
PsuI RGATCY 1 cut(s) 610
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 2 cut(s) 91, 572
RsaNI GTAC 2 cut(s) 90, 571
RseI CAYNNNNRTG 1 cut(s) 717
SacI GAGCTC 1 cut(s) 632
SaqAI TTAA 2 cut(s) 275, 342
SatI GCNGC 6 cut(s) 183, 222, 422, 436, 447, 461
Sau3AI GATC 5 cut(s) 28, 105, 406, 517, 610
SchI GAGTC 1 cut(s) 94
SduI GDGCHC 2 cut(s) 632, 727
SfaNI GCATC 4 cut(s) 411, 447, 472, 551
SfcI CTRYAG 3 cut(s) 253, 268, 614
SmiMI CAYNNNNRTG 1 cut(s) 717
Sse9I AATT 2 cut(s) 299, 321
SsiI CCGC 6 cut(s) 258, 400, 433, 458, 461, 540
SspI AATATT 1 cut(s) 793
SstI GAGCTC 1 cut(s) 632
TaaI ACNGT 5 cut(s) 254, 269, 372, 503, 575
TaiI ACGT 2 cut(s) 516, 789
TaqI TCGA 4 cut(s) 147, 405, 516, 626
TasI AATT 2 cut(s) 299, 321
TatI WGTACW 1 cut(s) 570
TauI GCSGC 1 cut(s) 463
TfiI GAWTC 2 cut(s) 64, 619
Tru1I TTAA 2 cut(s) 275, 342
Tru9I TTAA 2 cut(s) 275, 342
TscAI CASTG 6 cut(s) 50, 166, 257, 375, 476, 589
TseFI GTSAC 2 cut(s) 366, 467
TseI GCWGC 5 cut(s) 182, 221, 421, 435, 446
Tsp45I GTSAC 2 cut(s) 366, 467
TspDTI ATGAA 1 cut(s) 293
TspGWI ACGGA 3 cut(s) 259, 474, 612
TspRI CASTG 6 cut(s) 50, 166, 257, 375, 476, 589
VneI GTGCAC 1 cut(s) 723
VspI ATTAAT 1 cut(s) 275
XapI RAATTY 1 cut(s) 299
XmnI GAANNNNTTC 1 cut(s) 128
ZraI GACGTC 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.